대한본초학회지 (The Korea Journal of Herbology)

  • 제27권2호
  • /
  • Pages.37-42
  • /
  • 2012
  • /
  • 1229-1765(pISSN)
  • /
  • 2288-7199(eISSN)
대한본초학회 (The Korea Association of Herbology)
권호 표지
DOI QR코드

DOI QR Code

백자인(柏子仁)이 수컷 생쥐의 생식세포에 미치는 항산화 효과 연구

Study of Antioxidant Effects of Platycladi Semen on Male Reproductive cells

  • 김주성 (경희대학교 한의과대학 처방제형학교실) ;
  • 홍민정 (경희대학교 한의과대학 처방제형학교실) ;
  • 김도림 (경희대학교 한의과대학 처방제형학교실) ;
  • 박은화 (경희대학교 한의과대학 처방제형학교실) ;
  • 장문석 (경희대학교 한의과대학 처방제형학교실) ;
  • 박성규 (경희대학교 한의과대학 처방제형학교실)
  • Kim, Ju-Sung (Dept. of Prescriptionology, College of Oriental Medicine, Kyung Hee University) ;
  • Hong, Min-Jung (Dept. of Prescriptionology, College of Oriental Medicine, Kyung Hee University) ;
  • Kim, Do-Rim (Dept. of Prescriptionology, College of Oriental Medicine, Kyung Hee University) ;
  • Park, Eun-Hwa (Dept. of Prescriptionology, College of Oriental Medicine, Kyung Hee University) ;
  • Chang, Mun-Seog (Dept. of Prescriptionology, College of Oriental Medicine, Kyung Hee University) ;
  • Park, Seong-Kyu (Dept. of Prescriptionology, College of Oriental Medicine, Kyung Hee University)
  • 투고 : 2012.02.09
  • 심사 : 2012.03.16
  • 발행 : 2012.03.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Objectives : The purpose of this study is to examine the antioxidant effects on male mouse reproductive cells of the extract of Platycladi Semen. Methods : The extract was studied for diphenyl-picryl-hydrazyl (DPPH) radical scavenging activity, cell viability by a modified MTT assay, the effects on $H_2O_2$-induced cytotoxicity by MTT assay, lipid peroxidation by malondialdehyde (MDA) formation and super oxide dismutase (SOD), respectively. Results : The results showed that the extract scavenged DPPH radical in a dose-dependent manner by up to 74.87%. The cell viability of the extract was within 72~96% on Leydig cells and GC-2 cells at concentrations of 1, 5, 10, 50 and 100 ug/ml. The hydrogen peroxide-induced cytotoxicity of Leydig cells was protected to 72.09% by the extract at concentration of 100 ${\mu}g/ml$. The hydrogen peroxide-induced lipid peroxidation of MDA formation was decreased to 1.80 and 1.65 nmoles/mg protein by the extract at concentrations of 50 and 100 ${\mu}g/ml$. The extract at all concentrations, SOD activity was not significantly changed. Conclusions : In conclusion, the extract of Platycladi Semen has antioxidant effects on Leydig cells and protect male reproductive system against oxidative stress.

키워드

참고문헌

  1. The whole country a college of Oriental medicine. The joint textbook publish commission compilation. Herbalogy. Seoul:Younglimsa. 2008:534-5.
  2. Gukgajunguiyakgwalliguk <> Pyeonwihoepyeon. Junghwaboncho. Sanghae: Sanghaegwahakgisulchulpansa. 1999:2, 325-7.
  3. Heo J. Donguibogam. Seoul:Namsandang. 2004: 954.
  4. Hwang DY. Daeyeok Jeungmaek Bangyakhappyeon. Seoul:Namsandang. 1987:193, 352.
  5. Yang HO, Suh BY, Han BH. Isolation and characterization of platelet-activating factor receptor binding antagonist from Biota orientalis. Planta Med. 1995;61(1):37-40. https://doi.org/10.1055/s-2006-957995
  6. Yang HO, Kang YH, Suh DY, Kim YC, Han BH. Biological and pharmacological effects of pinusolide, a novel platelet activating factor antagonist. Planta Med. 1995;61(6):519-22. https://doi.org/10.1055/s-2006-959361
  7. Lee JY, Choi SW. Isolation and Purification of Tyrosinase Inhibitors from the Seeds of Thuja orientalis L. Korean J Postharvest Sci Technol. 2000;7(4):409-13.
  8. Berlin J, Witte L. Formation of mono- and diterpenoids by cultured cells of Thuja occidentalis. Phytochemistry. 1988;27(1):127-32. https://doi.org/10.1016/0031-9422(88)80602-2
  9. Kosuge T, Yokota M, Suqiyama K, Saito M, Iwata Y, Nakura M, Yamamoto T. Studies on anticancer principles in Chinese medicines. II. Cytotoxic principles in Biota orientalis (L.) Endl. and Kaempferia galanga L. Chem Pharm Bull.1985; 33(12):5565-67. https://doi.org/10.1248/cpb.33.5565
  10. Kim HG, Shim JS, Ju MS, Cho SH, Oh MS. Protective Effects of Thujae Semen against Neurotoxicity Induced by 6-hydroxydopamine in PC12 Cells. Kor. J. Herbology. 2008;23(3): 19-25.
  11. Guang-Hua Xu, In-Ja Ryoo, Young-Hee Kim, Soo-Jin Choo, Ick-Dong Yoo. Free radical scavenging and antielastase activities of flavonoids from the fruits of Thuja orientalis. Arch Pharm Res. 2009;32(2):275-82. https://doi.org/10.1007/s12272-009-1233-y
  12. Kim MS, Kim BY, Park CS, Yoon BD, Ahn SC, Oh WK, Ahn JS. Inhibitory Effect of Thujae orientalis Semen Extract on Pancreatic Lipase Activity. Journal of Life Science. 2006;16(2): 328-32. https://doi.org/10.5352/JLS.2006.16.2.328
  13. Ikuo Ikeda, Takayuki Oka, Kazunori Koba, Michihiro Sugano, Marcel S.F. Lie Ken Jie. 5c, 11c, 14c-Eicosatrienoic acid and 5c, 11c, 14c, 17c-Eicosatetraenoic acid of Biota orientalis seed oil affect lipid metabolism in the rat. 1992;27(7): 500-4. https://doi.org/10.1007/BF02536130
  14. WHO laboratory manual for the examination of human semen and sperm-cervical mucus interaction. 4th edition. Cambridge university press. Cambridge. England. 1999.
  15. Agarwal A, Saleh RA. Role of oxidants in male infertility: rationale, significance, and treatment. Urol Clin North Am 2002;29:817-27. https://doi.org/10.1016/S0094-0143(02)00081-2
  16. Agarwal A, Saleh RA, Bedaiwy MA. Role of reactive oxygen species in the pathophysiology of human reproduction. Fertil Steril. 2003;79: 829-43. https://doi.org/10.1016/S0015-0282(02)04948-8
  17. Esfandiari N, Sharma RK, Saleh RA, Thomas AJ Jr, Agarwal A. Utility of the nitrobluetetrazolium reduction test for assessment of reactive oxygen species production by seminal leukocytes and spermatozoa. J Androl. 2003;24: 862-70. https://doi.org/10.1002/j.1939-4640.2003.tb03137.x
  18. Agarwal A, Nallella KP, Allamaneni SS, Said TM. Role of antioxidants in treatment of male infertility: an overview of the literature. Reprod Biomed Online. 2004;8:616-27. https://doi.org/10.1016/S1472-6483(10)61641-0
  19. Sanocka D, Kurpisz M. Reactive oxygen species and sperm cells. Reprod Biol Endocrinol. 2004; 2:12. https://doi.org/10.1186/1477-7827-2-12
  20. Du HK. Donguisingyeyeonguhak. Seoul:KyuHee University Press. 1994:368-70.
  21. Chin ST. Seoksilbirok. Beijing:Inminwisaengchulpansa. 1984:162.
  22. Son SM. Bigeupcheongeumyobang. Beijing: Inminwisaengchulpansa. 1982:16.
  23. Yeo YB. Junguichiryonaebunbidaesabyeong. Jeolgang:Jeolganggwahakgisul chulpansa. 1992:120-45.
  24. Shin CH. Seonggineungjangaewa burimjeung. Seoul:Jeontonguihagyeonguso. 1993:110-210.
  25. Oh B. Sinnongbonchogyeong. Seoul:Uiseongdang. 2003:110.
  26. Do HK. Myeonguibyeollok. Beijing: Inminwisaengchulpansa. 1986:19.
  27. Heo J. Gugyeokjeungbodonguibogam. Seoul: Beobinmunhwasa. 1991:954.
  28. Paick JS. Role of Reactive Oxygen Species in Male Infertility. Kor J Androl. 2003;21(1):1-11.
  29. David R. Mechanisitic toxicology. A radical perspective. J Pharm. pharmacol. 1989;41: 505-11. https://doi.org/10.1111/j.2042-7158.1989.tb06516.x
  30. The Korean Urological Association. Textbook of Urology. 3rd. Seoul:Goryeouihak. 2001:508.
  31. Rao B, Soufir JC, Martin M, David G. Lipid peroxidationin human spermatozoa as related to mid-piece abnormality and motility. Gamate Res. 1989;24:127-33. https://doi.org/10.1002/mrd.1120240202

피인용 문헌

  1. Current status on the development of molecular markers for differentiation of the origin of Angelica spp. vol.44, pp.1, 2017, https://doi.org/10.5010/JPB.2017.44.1.012