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Study on the Effects of Morphology, Preservation and Reproductive Cycle of In Vitro Developmental Rate of Cats Oocytes  

전연화 (충남대학교 수의과대학)
이명헌 (수의과학검역원)
김상근 (충남대학교 수의과대학)
Publication Information
Journal of Embryo Transfer / v.18, no.2, 2003 , pp. 109-113 More about this Journal
Abstract
The study was carried out to investigate the effects of morphology, preservation and reproductive cycle of oocytes in vitro maturation of cats oocytes and development of IVM embryos. The results were summarized as follows : 1. Nuclear status of GV and MI of in vitro cultured(24 h) oocytes with and whithout cumulus cells were 74.3% and 25.7%, 28.6% and 11.4%, 77.1% and 5.7%, respectively, The rate of oocytes with cumulus cells was higher than that of denuded oocytes. 2. Nuclear status of GV and MI of in vitro cultured(24 h) oocytes recovered from ovaries collected at different stages of the reproductive cycle(inactive, follicular and luteal) were 88.6% and 6.5%, 60.0% and 11.4%, 77.1% and 5.7%, respectively. 3. Nuclear status of fresh and salts-stored oocytes with and whithout cumulus cells were 74.3%, 25.7% and 37.1%, 11.4% and 57.1%, 13.3%, 17.1%, 3.3%, respectively. The rate of oocytes with cumulus cells(13.3%∼74.3%) was higher than that of denuded oocytes(3.3%∼57.1%).
Keywords
cats; morphology; fresh and salt-stored; reproductive stage;
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1 Fazeli AR, Steenweg W, Bevers MM, de Loos FAM, van den Broek J and Colenbrander B. 1993. Development of a sperm zona pellucida binding assay for bull semen. Vet. Rec., 132:397-410
2 Goodowe KL, Hay M and King WA. 1991. Nuclear maturation of domestic cat ovarian oocytes in vitro. BioI. Reprod., 45:466-470   DOI   ScienceOn
3 Kim SK. 2001. Studies on the viability of frozen removed seminal plasma by saline (RSP-S) and trisbuffer(RSP-T) semen of small species dogs. Korean J. Anim. Reprod., 25(3):269-275
4 Spindler RE and Wildt DE. 1999. Circannual variations in intraovarian oocyte but not epididymal sperm quality in the domestic cat. bioI. Reprod., 61:188-194   DOI   ScienceOn
5 Wood TC, Byers AP, Jennette BE. and Wildt DE. 1995. Influence of protein and hormone supplementation on in vitro maturation and fertilization of domestic cat eggs. J. Reprod. Fertil., 104:315-323   DOI
6 Hay MA, King WA., Gartley CJ, Leibo SP and Goodrowe KL. 1997. Effects of cooling, freezing and glycerol on penetration of oocytes by spermatozoa in dogs. J. Reprod. Fertil., 51:99-108
7 Karja NWK, Otoi T, Murakami M, Fahrudin M. and Suzuki T. 2002. In vitro maturation, fer-tilization and development of domestic cat oocytes recovered form ovaries collected at three stages of the reproductive cycle. Theriogenology, 57(9):2289-2   DOI   ScienceOn
8 Freistedt P, Stojkovic M and Wolf E. 2001. Efficient in vitro production of cat embryos in modified synthetic oviduct fluid medium : effect of season and ovarian status. BioI. Reprod., 65:9-13   DOI   ScienceOn
9 Codde JM and Berger T. 1995. In vivo fertility of rams in relation to sperm zona pellucida binding and sperm zona pellucida penetration of ovine oocytes. Theriogenology, 44:901-906   DOI   ScienceOn
10 Johnston LA, Donoghue AM. O'Brien SJ and Wildt DE. 1991. Influence of temperature and gas atmosphere on in vitro fertilization and embryo development in the domestic cat. J. Reprod. Fertil., 92:377-382   DOI
11 Ivanova M and Mollova M. 1993. Zona- penetration in vitro test for evaluating boar sperm fertility. Theriogenology, 40:397-410   DOI   ScienceOn
12 Goodrowe KL and Hay M. 1993. Characteristics and zona binding ability of fresh and cooled domestic cat epididymal spermatozoa. Theriogenology, 40:967-975   DOI   ScienceOn
13 Farstad W. 2000. Current state in biotechnology in canine and feline reproduction. Anim. Reprod. Sci., 61:375-387   DOI   PUBMED   ScienceOn
14 Maertinez E, Vazquez JM., Matas C, Roca J, Coy P and Gadea J. 1993. Evaluation of boar spermatozoa penetrating capacity using pig oocytes at the germinal vesicle stage. Theriogenology, 44:901-906   DOI   ScienceOn
15 Otoi TM., Murakami A, Ooka NWK, Karja and Suzuki T. 2001. Effects of size and storage temperature on meiotic competence of domestic cat oocytes. Vet. Rec., 148: 350-355
16 Howard JG, Bush M and Wildt DE. 1991. Teratospermia in domestic cats compromises penetration of zona-free hamster ova and cat zonae pellucidae. J. Androl., 12:36-45
17 Hewitt DA and England GCW. 1999. Synthetic oviductal fluid and oviductal cell coculture for canine oocytes maturation in vitro. Anim. Reprod. Sci., 55(1):63-75   DOI   ScienceOn
18 Pope CE, McRae MA, Plair BL, Keller GL and Dresser BL. 1997. In vitro and in vivo development of embryos produced by in vitro maturation and in vitro fertilization of domestic cat follicular oocytes. Gam. Res., 24: 343-356   DOI