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http://dx.doi.org/10.6116/kjh.2012.27.2.37

Study of Antioxidant Effects of Platycladi Semen on Male Reproductive cells  

Kim, Ju-Sung (Dept. of Prescriptionology, College of Oriental Medicine, Kyung Hee University)
Hong, Min-Jung (Dept. of Prescriptionology, College of Oriental Medicine, Kyung Hee University)
Kim, Do-Rim (Dept. of Prescriptionology, College of Oriental Medicine, Kyung Hee University)
Park, Eun-Hwa (Dept. of Prescriptionology, College of Oriental Medicine, Kyung Hee University)
Chang, Mun-Seog (Dept. of Prescriptionology, College of Oriental Medicine, Kyung Hee University)
Park, Seong-Kyu (Dept. of Prescriptionology, College of Oriental Medicine, Kyung Hee University)
Publication Information
The Korea Journal of Herbology / v.27, no.2, 2012 , pp. 37-42 More about this Journal
Abstract
Objectives : The purpose of this study is to examine the antioxidant effects on male mouse reproductive cells of the extract of Platycladi Semen. Methods : The extract was studied for diphenyl-picryl-hydrazyl (DPPH) radical scavenging activity, cell viability by a modified MTT assay, the effects on $H_2O_2$-induced cytotoxicity by MTT assay, lipid peroxidation by malondialdehyde (MDA) formation and super oxide dismutase (SOD), respectively. Results : The results showed that the extract scavenged DPPH radical in a dose-dependent manner by up to 74.87%. The cell viability of the extract was within 72~96% on Leydig cells and GC-2 cells at concentrations of 1, 5, 10, 50 and 100 ug/ml. The hydrogen peroxide-induced cytotoxicity of Leydig cells was protected to 72.09% by the extract at concentration of 100 ${\mu}g/ml$. The hydrogen peroxide-induced lipid peroxidation of MDA formation was decreased to 1.80 and 1.65 nmoles/mg protein by the extract at concentrations of 50 and 100 ${\mu}g/ml$. The extract at all concentrations, SOD activity was not significantly changed. Conclusions : In conclusion, the extract of Platycladi Semen has antioxidant effects on Leydig cells and protect male reproductive system against oxidative stress.
Keywords
Platycladi Semen; diphenyl-picryl-hydrazyl (DPPH); MTT assay; hydrogen peroxide ($H_2O_2$); lipid peroxidation (LPO); Leydig cells;
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Times Cited By KSCI : 1  (Citation Analysis)
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