This study was carried out to investigate the performance of laying period of two-crossbred of Korean native chickens for producing laying hens. A total of four hundred eighty female 2-crossbred chicks that restored strains and were aboriginal at National Institute of Animal Science. There were four crossbreds (4 replications/crossbred, 30 birds/replication) as A) C strain ${\times}$ Y strain, B) C strain ${\times}$ L strain, C) C strain ${\times}$ G strain, and D) C strain ${\times}$ W strain, respectively. There were no significant difference on age at first egg among crossbreds (P>0.05). Egg weight and body weight of B crossbred at first egg was higher than other crossbreds (P<0.05). Body weight of B crossbred was the highest 20 to 72 weeks (P<0.05), and C and D crossbreds were lower compared to A and B crossbreds (P<0.05). Average feed intake of B crossbred was the highest among crossbreds (P<0.05), and that of A crossbred was higher compared to C and D crossbreds (P<0.05). Weekly feed intake of four crossbreds decreased from 50 weeks. Weekly egg weight of B crossbred was the highest and that of D crossbred was the lowest among crossbreds (P<0.05). Feed conversion ratio of A and B crossbreds was lower than that of C and D crossbreds. Hen-house egg production of C crossbred was the highest among crossbreds until 26 weeks old (P<0.05), but there was no significant difference among crossbreds from at the age of 26 weeks (P>0.05). Hen-day egg production decreased after at the age of 38 weeks. Weekly egg production of A and B crossbreds was higher compared to C and D crossbreds at the age of 68~72 weeks (P<0.05). These results suggested the basic data on the record of laying period of 2-crossbreed Korean Native Chickens for producing laying hens.
This study investigated the effect of different light sources in the poultry house on performance, meat quality, and blood composition of finishing broilers. Two hundred and forty male broilers (1-day-old, 42.2±0.1 g) were divided into three groups and subjected to different light source treatments (incandescent, LED, and fluorescent lamps) from 3 weeks of age (four replications/treatment, 20 birds/replication). After breeding for 6 weeks, the carcass yield and meat quality of broilers with similar body weight (BW; 3.4±0.07 kg) were investigated, and blood components were analyzed. Corn-soybean meal-based feed was provided as starter (CP 22.5%, ME 3,020 kcal/kg), early (CP 18.5%, ME 3,050 kcal/kg), and finishing (CP 18%, ME 3,100 kcal/kg). Performance, carcass yield, meat quality, and blood components were evaluated. BW, BW gain, feed intake, and feed conversion ratio did not show any significant differences among treatments. There was no significant difference on live weight and carcass yield among treatments. There was no significant difference on meat color, shear force, and water holding capacity; however, cooking loss at 17.2% was the highest in the LED treatment (P<0.05). There was no significant difference on blood components except for glucose (blood biochemistry component) among treatments. Glucose was 234.5 mg/dL, 256.9 mg/dL, and 250.1 mg/dL in the three treatments, respectively, with a significant difference between incandescent and LED treatments (P<0.05). These results are used useful as basic data for investigating the effect of lighting in broilers production.
LEE Sang-Min;KIM Dong-Ju;KIM Joong Kun;HHR Sung Bum;LEE Jong Kwan;LIM Han Kyu
Korean Journal of Fisheries and Aquatic Sciences
/
v.33
no.5
/
pp.463-468
/
2000
This study was conducted to investigate the effects of Kluyveromyces fragilis, Candida utilis and brewer's yeast as an additive in formulated diets containing different fish meal levels ($55{\%} and 45{\%}$) on growth and body composition of juvenile Korean rockfish (Sebastes schlegeli). Juvenile rockfish (average weight, 2.6 g) were distributed into 24 tanks with 3 replication groups and fed eight isoproteic ($41{\%}$) and isolipidic ($11{\%}$) diets containing each level ($3{\%}$) of K. frayilis, C. utilis and brewer's yeast as an additive. Survival rate of fish was not affected by dietary fish meal level and yeast kind (P > 0.05). Weight gain of fish fed the control and diets containing C. utilis and brewer's yeast was not influenced by fish meal level (P > 0.05), Weight gain of fish fed the $55{\%}$ fish meal diet containing C. utilis and $45{\%}$ fish meal diets containing K. fragilis and C. utilis was lower than that of fish fed the control diet (P < 0.05). Feed efficiency and protein efficiency ratio of fish fed the control and diet containing brewer's yeast were not affected by fish meal level, but these value of fish fed the diets containing K, fragilis and C. utilis were lower in $45{\%}$ fish meal diets than in $55{\%}$ fish meal diets. Hepatosomatic index and liver lipid content of fish fed the diet containing C. utilis were decreased regardless of fish meal level (P < 0.05), Hemoglobin, hematocrit, red blood cell, plasma total protein and plasma total glucose were not affected by dietary fish meal levels and yeast supplements (P > 0.05). From the above results, it can be concluded that yeast supplement in this formulated diet for growth of juvenile rockfish is not necessary.
Park, Ji Hye;Yun, Jisoo;Heo, Jeong;Hwang, Tae Ho;Kwon, Sang Mo
Journal of Life Science
/
v.26
no.7
/
pp.835-846
/
2016
Chemo-resistance is the biggest issue of effective cancer therapy. ABCG2 is highly correlated with multi-drug resistance, and represent a typical phenotype of multiple cancer stem-like cells. Accumulating evidence recently reported that oncolytic viruses represent a new strategy for multiple aggressive cancers and drug resistant cancers including cancer stem cell-like cells and ABCG2 expressing cells. In this study, we generated an evolutionally engineered vaccinia virus, SLJ-496, for drug-resistant cancer therapy. We first showed that SLJ-496 treatment enhanced tumor affinity using cytopathic effect assay, plaque assay, as well as cell viability assay. Next, we clearly demonstrated that in vitro SLJ-496 treatment represents significant cytotoxic effect in multiple cancers including colorectal cancer cells (HT-29, HCT-116, HCT-8), gastric cancer cells (AGS, NCI-N87, MKN-28), Hepatocellular carcinoma cells (SNU-449, SNU-423, SNU-475, HepG2), as well as mesothelioma cell (NCI-H226, NCI-H28, MSTO-221h). Highly ABCG2 expressing HT-29 cells represent cancer stem like phenotype including stem cell marker expression, and self-renewal bioactivities. Interestingly, we demonstrated that in vitro treatment of SLJ-496 showed significant cytotoxicity effect, as well as viral replication capacity in ABCG2 overexpressing cell. In addition, we also demonstrated the cytotoxic effect of SLJ-496 in Adriamycin-resistant cell lines, SNU-620 and ADR-300. Taken together, these findings provide us a pivotal clue that cancer therapy using SLJ-496 vaccinia virus might be new therapeutic strategy to overcome ABCG2 expressing cancer stem-like cell and multiple chemo-resistance cancer cells.
Journal of The Korean Society of Grassland and Forage Science
/
v.23
no.4
/
pp.255-264
/
2003
A study was conducted to determine the effects of pig slurry application on forage yield and growth of fazing Hanwoo heifer in a mixed pasture. To each of three treatments 1.4ha were alloted a control applied with only chemical fertilizer (N-P-K=150-150-120kg/ha), two pig slurry lots applied with the amount to allow 100% (150 kg/ha) or 150% (225 kg/ha) of N used in the control. A randomized block design was used without replication. Cattle were allowed to graze continuously during the experimental period. Results obtained were as follows: Total dry matter yield was 16,291, 15,632 and 16,320 kg/ha for chemical fertilizer. pig slurry 100% and 150%, respectively. The pasture was dominated by perenial ryegrass during the first gazing season, but by orchard grass and perenial ryegrass (60∼70%) and red clover (20∼30%) during the second grazing season. Average gazing rate per ha was 2.75∼2.76 animal units and daily weight gain of grazing cattle was not different among treatments ranging from 0.563 to 0.580 kg. Total weight gain of grazing cattle per ha during the grazing period was 541, 541 abd 555 kg for chemical fertilizer, pig -slurry 100 and 150%, respectively. RBC, WBC, total protein and albumin etc. concentrations in blood were normal in all treatments.
Journal of The Korean Society of Grassland and Forage Science
/
v.39
no.2
/
pp.81-88
/
2019
This study was carried out to investigate the growth characteristics, yield and feed value of Sorghum${\times}$Sudangrass hybrid for silage according to the drainage depths in the paddy field of lowland. The experimental design was arranged in a randomized block design with four treatments and three replication. The drainage depths of four treatments were 0cm, 20cm, 40cm and 60cm, respectively. Plant length, leaf length and leaf number were not significantly different, but leaf width increased as the higher the drainage depth(p<0.05). The number of dead leaf was higher in the order of $60cm>0cm{\geq}40cm{\geq}20cm$ treatment(p<0.05). Green degree was higher in the order of 20cm > 40cm > 0cm > 60cm treatment(p<0.05). Stem diameter and stem hardness increased significantly as drainage depth increased from 0cm to 60cm(p<0.05). Also, fresh yield, dry matter yield and TDN yield increased as the higher the drainage depth(p<0.05). Crude protein and TDN content were the highest in 40cm treatment(p<0.05). Crude ash was higher in the order of 20cm > 40cm > 0cm > 60cm treatment(p<0.05). ADF and NDF content were the highest in 0cm treatment(p<0.05). Total mineral content was higher in the order of 20cm > 0cm > 40cm > 60cm(p<0.05). Free sugar content(fructose, glucose and sucrose) was the highest in 0cm treatment(p<0.05). Total amino acid(EAA+NEAA) was higher in 40cm than the other treatments(p<0.05). There is a difference in the content of ingredients(crude protein, TDN, mineral, free sugar and amino acid) according to the treatments. But considering dry matter yield and TDN yield, Sorghum${\times}$Sudangrass hybrid cultivation is advantageous to set the drainage depth of about 60cm in the paddy field of lowland.
Woo-Do Lee;Jiseon Son;Hyun-Soo Kim;Hee-Jin Kim;Yeon-Seo Yun;Hwan Ku Kang;Woncheoul Park;Han Ha Chai;Eui-Chul Hong
Korean Journal of Poultry Science
/
v.50
no.2
/
pp.101-108
/
2023
This study was conducted to investigate the appropriate levels of crude protein (CP) and phytase in the diet of broiler chicks in order to reduce nitrogen and phosphorus contents in feces while maintaining performance of broilers. Six hundred forty-eight 1-day-old male broilers (41.9±0.91 g) had a total of 3 × 3 complex factor of 3 levels of CP (22%, 21%, 20%) and 3 levels of phytase (1,000, 800, 500 FTU/kg) in the diet. Divided into 9 treatments, 4 replications per treatment, 18 birds per replication, were completely randomly assigned and reared in a metabolic cage for 7 days. Seven-day-old body weight (BW) and body weight gain (BWG) of broilers were significantly lower at CP 20% treatment (P<0.05), and feed conversion ratio (FCR) was significantly lower at CP 21% and phytase 800 FTU/kg treatment (P<0.05). Nitrogen and phosphorus contents in chicken excreta were significantly lower in CP 20% and phytase 500 FTU/kg treatment, respectively (P<0.05). Interactions between CP and phytase in the feed were shown for nitrogen and phosphorus in feces (P<0.05). In conclusion, considering the broiler performance and excretion contents of nitrogen and phosphorus, it is thought that CP and phytase levels of broiler chicks diet can be reduced by 21% and 800 FTU/kg, respectively.
This study was conducted to investigate the effect of moisture absorbent (MA) application timing for litter management on broiler performance, blood cell characteristics, litter moisture content, incidence of footpad dermatitis (FPD), and economics analysis. Treatment include untreated control (NC), 3-week-old litter treatment (PC), 0-week-old (W1), 0 and 3-week-old (W2), 3-week-old (W3) application of moisture absorbent. Six hundred eighty broilers (1-day-old, 42.0±0.24 g) were divided into 5 treatments (4 replications per treatment, 34 birds per replication) and raised for 5 weeks in a floor (2 m2 per pen). There was no significant difference among treatments in performance, blood cell characteristics, and H/L ratio according to the application period of litter and moisture absorbent. The litter moisture content and the FPD score were significantly decreased in the litter and moisture absorbent treatments at the age of 5 weeks (P<0.05). The FPD score of broilers was lowest in PC treatment compared to NC treatment (P<0.05). The incidence of FPD was lower in PC and W3 treatments compared to other treatments, and that was the highest in NC treatment. As a result of analyzing the economic feasibility, the highest expenditure occurred in PC treatment, and the lowest expenditure occurred in W3 treatment. Income was the highest in W3 treatment, and lowest in NC treatment. The profit was 185,859 won (1,367 won/unit), the highest in the W3 treatment. In conclusion, when MA was applied to the litter of broiler house at the age of 3 weeks, the litter moisture content and FPD were improved.
Journal of The Korean Society of Grassland and Forage Science
/
v.30
no.1
/
pp.15-24
/
2010
This study was conducted to investigate the effects of swine manure (SM) application with additional nitrogen (N) fertilizer on productivity of corn and environmental pollution in com cultivation soil. The experiment was conformed in lysimeter which was constructed with 30 cm diameter, and 100 cm height. Swine manures used in this study were the compost of swine manure fermented with sawdust (SMFWS) and soft rice hulls treated with high temperature and high pressure (SRH), and swine slurry (SS). The application rates of the additional N, as urea, with swine manure were 50 and 100 kgN/ha on each plot. This study was arranged in completely randomized design with three replication. DM yields in SM treatments with mineral N were increased significantly compared to those in SM treatment without mineral N (zero-mineral N) (P<0.05) and increased as the rate of mineral N application increased (P<0.05). DM yields in SMFWS and SS treatments with mineral 100 kgN/ha showed trends similar to those of chemical fertilizer (control) but higher than those of 50 kg N/ha. DM yields tended to be higher in SMFWS and SS treatments than in SRH treatment. Total N contents in SMFWS, SRH and SS treatments with mineral N were increased, compared with SM treatment without mineral N. N contents in SM treatments with mineral N were lower than those of chemical fertilizer treatment, but N content of chemical fertilizer treatment showed trends similar to that of SS treatments with mineral 100 kgN/ha. $NO_3$-N concentration in SM treatments with mineral N were increased significantly, compared to those in SM treatment without mineral N and in chemical fertilizer (P<0.05). $NH_4$-N concentrations in SMFWS and SS treatments with mineral 100 kgN/ha showed trends similar to those of chemical fertilizer, but higher than those of 50 kg N/ha. $PO_4$-P concentration in SM treatments with mineral N were increased significantly, compared to those in SM treatment without mineral N (P<0.05). $PO_4$-P concentration in chemical fertilizer treatment showed trends similar to that of SS treatments with mineral 100 kgN/ha. The concentrations of $NO_3$-N $NH_4$-N and $PO_4$-P increased as the rate of mineral N application increased (P<0.05). The concentrations of $NO_3$-N $NH_4$-N and $PO_4$-P were highly elevated in the concentrated rainy season in the early stage among experimental period. The maximum $NO_3$-N $NH_4$-N and $PO_4$-P concentrations in the leaching water were 3.46 mg/L, 1.11 mg/L and 0.14 mg/L, respectively.
Purpose: To evaluate the efficacy of interferon alpha therapy with or without prednisolone in children with chronic hepatitis B. Methods: Twenty-eight children (22 boys, 6 girls, mean age 130 months) had seropositive results for HBsAg, HBeAg and HBV DNA; 11 had chronic persistent hepatitis and 17 had chronic active hepatitis. The patients were divided into two groups depending upon their inflammatory activity on liver biopsy, pretreatment serum ALT levels and HBV DNA levels. Fourteen children (group 1: chronic active hepatitis, ALT ${\geq}$ 100 IU/L and HBV DNA ${\leq}$ 100 pg/$300\;{\mu}L$) received interferon alpha 2a 5 $MU/m^2$ of body surface three times weekly for 6 months. Fourteen children (group 2: chronic persistent hepatitis or chronic active hepatitis with ALT < 100 IU/L or HBV DNA > 100 pg/$300\;{\mu}L$) received prednisolone in decreasing daily doses of 60 mg/$m^2$, 40 mg/$m^2$, and 20 mg/$m^2$, each for 2 weeks, followed after 2 weeks by interferon alpha 2a on the same schedule. At the end of therapy, 3 end points were analyzed: HBeAg seroconversion, serum ALT normalization rate and clearance of serum HBV DNA. Results: At the end of treatment, HBe antigen-to antibody seroconversion was higher but not more significant in group 1 than group 2 (71.4% vs. 50.0%). Only one patient in group 2 who lost HBeAg, also cleared HBsAg. ALT normalization was similar in both groups (64.3% in group 1 vs. 55.6% in group 2). Clearance of serum HBV DNA was observed in 78.6% of patients in group 1 and 64.3% in group 2, but no significant differences. Complete response was similarly achieved in both groups (57.1% in group 1 vs. 50.0% in group 2). Interferon alpha therapy with prednisolone priming was well tolerated and all children finished therapy. Conclusion: The combined therapy with prednisolone followed by interferon alpha may be safe and effective in inducing a serological and biochemical remission of the disease in approximately 50% of children with chronic hepatitis B and with a high level of viral replication and less active disease. However, a controlled study should be performed to confirm these results.
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