• Molecules and Cells
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• 제23권3호
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• pp.357-362
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• 2007

There is an increasing demand for high throughput (HTP) methods for gene analysis on a genome-wide scale. However, the current repertoire of HTP detection methodologies allows only a limited range of cellular phenotypes to be studied. We have constructed two HTP-optimized expression vectors generated from the red fluorescent reporter protein (RFP) gene. These vectors produce RFP-tagged target proteins in a multiple expression system using gateway cloning technology (GCT). The RFP tag was fused with the cloned genes, thereby allowing us localize the expressed proteins in mammalian cells. The effectiveness of the vectors was evaluated using an HTP-screening system. Sixty representative human C2 domains were tagged with RFP and overexpressed in HiB5 neuronal progenitor cells, and we studied in detail two C2 domains that promoted the neuronal differentiation of HiB5 cells. Our results show that the two vectors developed in this study are useful for functional gene analysis using an HTP-screening system on a genome-wide scale.

• ALGAE
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• 제36권4호
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• pp.315-326
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• 2021

Ion channels are membrane protein complexes mediating passive ion flux across the cell membranes. Every organism has a certain set of ion channels that define its physiology. Dinoflagellates are ecologically important microorganisms characterized by effective physiological adaptability, which backs up their massive proliferations that often result in harmful blooms (red tides). In this study, we used a bioinformatics approach to identify homologs of known ion channels that belong to 36 ion channel families. We demonstrated that the versatility of the dinoflagellate physiology is underpinned by a high diversity of ion channels including homologs of animal and plant proteins, as well as channels unique to protists. The analysis of 27 transcriptomes allowed reconstructing a consensus ion channel repertoire (channelome) of dinoflagellates including the members of 31 ion channel families: inwardly-rectifying potassium channels, two-pore domain potassium channels, voltage-gated potassium channels (K_v), tandem K_v, cyclic nucleotide-binding domain-containing channels (CNBD), tandem CNBD, eukaryotic ionotropic glutamate receptors, large-conductance calcium-activated potassium channels, intermediate/small-conductance calcium-activated potassium channels, eukaryotic single-domain voltage-gated cation channels, transient receptor potential channels, two-pore domain calcium channels, four-domain voltage-gated cation channels, cation and anion Cys-loop receptors, small-conductivity mechanosensitive channels, large-conductivity mechanosensitive channels, voltage-gated proton channels, inositole-1,4,5-trisphosphate receptors, slow anion channels, aluminum-activated malate transporters and quick anion channels, mitochondrial calcium uniporters, voltage-dependent anion channels, vesicular chloride channels, ionotropic purinergic receptors, animal volage-insensitive cation channels, channelrhodopsins, bestrophins, voltage-gated chloride channels H⁺/Cl^- exchangers, plant calcium-permeable mechanosensitive channels, and trimeric intracellular cation channels. Overall, dinoflagellates represent cells able to respond to physical and chemical stimuli utilizing a wide range of G-protein coupled receptors- and Ca²⁺-dependent signaling pathways. The applied approach not only shed light on the ion channel set in dinoflagellates, but also provided the information on possible molecular mechanisms underlying vital cellular processes dependent on the ion transport.

• 한국콘텐츠학회논문지
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• 제18권12호
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• pp.372-382
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• 2018

본 연구는 고든의 음악 학습 이론을 스즈키 바이올린 교육에 통합하여 어린이들을 위한 바이올린 교수 학습모델을 만드는 것에 그 목적을 두고 있다. 스즈키 교육에 음악 학습 이론의 원리를 적용하는 주된 목적을 학생들의 오디에이션 개발에 두고 있으며, 스즈키 바이올린 교육과 고든의 음악 학습 이론 원리와의 관련성을 토대로, 스즈키 철학과 커리큘럼의 틀 안에서 오디에이션을 어떻게 가르칠 수 있는지 수업예시로 보여 줄 수 있도록 하였다. 이번 연구는 음악 학습 이론을 적용 할 수 있는 방법들 중 한 가지 가능성만을 제시하고 있으며, 스즈키 바이올린 교육에 음악 학습 이론을 체계적으로 적용하기 위해서는 제시된 수업 계획의 효과와 강점에 대한 추가 연구가 필요하며 스즈키 레퍼토리에서 사용 된 조성 및 리듬 패턴에 대한 보다 완벽한 안내서가 필요하다. 악기를 연주하는 학생들의 오디에이션 능력이 함양된다면, 기계적인 움직임이 아닌, 그들이 듣고, 연주하고, 작곡하는 음악에 대한 이해를 더 깊이 할 수 있을 것으로 기대된다.

• 비교문화연구
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• 제25권
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• pp.421-452
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• 2011

This writing is about an effort to try to preserve and transmit the materials related to Jing-Ju(京劇) who is the Chinese traditional theatrical art. The plan to preserve the materials of performing arts is very important because its collection and preservation about them are much more particular than other genres. China got the great results in this field because of China Jing-Ju Yinpeixiang (中國京劇音配像) project. Jing-Ju Yinpeixiang project is a kind of project to restore and preserve the materials which reproduced Jing-Ju repertoire by inserting the performance method of Yinpeixiang (音配像: put together images to sounds) by later actors and actresses of Jing-Ju in recordings of 1950s to 1960s by remained famous ones. Also, this is the large scale culture project that more than 170 institutions and more than 3000 persons participated in during the total 21 years that it first started in 1985 and was completed in the end of 2006. Thanks to this project, China got to have the almost perfect materials which reproduced live performances to add the images of junior actors and actresses who accurately imitated performances of 115 famous actors and actresses including each type of role, schools and great actors from 1907 to the end of 20th century in their voices. This is the only method to restore the materials in this world and very creative. It's been 15 years since TV broadcasting first showed its fruition to Chinese people. The Chinese government gave the positive judgement on this project and prepared to hold the big event which celebrated itself this year. But very little is known of this process and there is no any study in Korea. This writing tried to clarify what Jing-Ju Yinpeixiang project is, what it has meant to Chinese people and what results it has gotten after it's been 15 years since the first show was on TV.

• 농업과학연구
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• 제48권3호
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• pp.643-654
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• 2021

Phytophthora capsici is one of the most destructive hemibiotrophic pathogens; it can cause blight in chili peppers, and secrete various effector proteins to infect the plants. These effectors contain an N-terminal conserved RXLR motif. Here, we generated full-length RXLR effector coding genes using primer pairs, and cloned them into the pGR106 vector for in planta expression. Two of these genes, PcREK6 and PcREK41 (P. capsici RXLR effector from the Korea isolate), were further characterized. PcREK6 and PcREK41 genes showed that they encode effector proteins with a general modular structure, including the N-terminal conserved RXLR-DEER motif and signal peptide sequences. PcREK6 and PcREK41 expressions were strongly induced when the chili pepper plants (Capsicum annuum) were challenged with P. capsici. These results provide molecular evidence to elucidate the virulence or avirulence factors in chili pepper. Our results also showed that two effectors induce hypersensitive response (HR) cell death when expressed in chili leaves. Cell death suppression assays in Nicotiana benthamiana revealed that most effectors could not suppress programmed cell death (PCD) triggered by Bcl-associated X (BAX) or Phytophthora infestans elicitin (INF1). However, PcREK6 fully suppressed PCD triggered by BAX, while PcREK41 partially suppressed PCD triggered by INF1 elicitin. These results suggest that PcREK effectors from P. capsici interact with putative resistance (R) proteins in planta, and different effectors may target different pathways in a plant cell to suppress pattern-triggered immunity (PTI) or effector-triggered immunity (ETI).

• 한국버섯학회지
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• 제19권1호
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• pp.33-40
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• 2021

최근 Flammulina 종들에 대한 유전체 염기서열 분석결과가 보고되었고, 그로 인해 다양한 유전자 정보가 밝혀지고 있다. 본 연구에서는 Flammulina elastica 전체 유전체 서열의 laccase 유전자를 동정하고 구조적 특징 분석을 수행하고자 하였다. 유전체 분석 및 생물정보분석을 통하여 F. elastica 유전체 내 3개의 laccase 유전자(Fe-lac1, Fe-lac2, Fe-lac3)를 확인하였고, 이들 유전자 내에는 10개의 히스티딘 잔기와 1개의 시스테인 잔기를 가지는 구리 이온 결합 영역과 4개의 시스테인 잔기를 가지는 이황화결합 형성 부위가 존재하는 것을 확인하였다. 1,548~1,602 bp의 laccase 유전자에 대한 전장 cDNA 염기서열 분석을 통하여 12~16개의 인트론이 존재하는 것을 확인되었으며, N-말단으로부터 17~22 bp의 사이에 신호펩타이드가 존재하는 것이 확인되었다. 본 연구를 통하여 F. elastica의 laccase 유전자를 최초로 동정하여 구조적 특징을 분석하였고, 이러한 결과는 F. elastica의 바이오매스 분해에 대한 이해를 돕는데 활용될 것으로 사료된다.

• 한국콘텐츠학회논문지
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• 제21권12호
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• pp.132-139
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• 2021

본 코퍼스 기반 연구는 통사론 영역에서 자주 등장하는 학술어휘들을 목록화하고 추출된 단어 목록을 Coxhead(2000)의 학술어휘 목록(AWL) 및 West(1953)의 기본어휘 목록(GSL)과 비교하여 통사론 코퍼스 내의 어휘 분포와 범위를 조사하였다. 이를 위해 영어교육 전공자들이 주로 사용하는 필수 통사론 전공 서적을 546,074 단어 수준의 전문 코퍼스로 구축한 다음 AntWordProfiler 1.4.1로 분석하였다. 빈도를 기준으로 분석한 결과 16회 이상 등장한 학술어휘는 288개(50.5%), 15회 이하 등장한 학술어휘는 218개(38.2%)로 나타났다. AWL과 GSL의 출현 범위는 각각 9.19%와 78.92%로 나타났으며 GSL과 AWL을 포함한 비중은 전체 토큰의 88.11%에 달하였다. AWL이 광범위한 학술 요구를 충족시키는데 중추적인 역할을 할 수 있다는 점을 감안할 때, 본 연구는 학문 문식성과 학업 능력을 향상시키기 위한 방안으로 학문 분야별 학술 어휘목록을 편성할 필요가 있음을 강조하였다.

• Journal of Animal Science and Technology
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• 제63권6호
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• pp.1423-1432
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• 2021

To elucidate the role and mechanism of microbes, we combined culture-dependent and culture-independent approaches to investigate differences in gut bacterial composition between sows and weaned pigs. Under anaerobic conditions, several nonselective and selective media were used for isolation from fecal samples. All isolated bacteria were identified and classified through 16S rRNA sequencing, and the microbiota composition of the fecal samples was analyzed by metagenomics using next generation sequencing (NGS) technology. A total of 278 and 149 colonies were acquired from the sow and weaned pig fecal samples, respectively. Culturomics analysis revealed that diverse bacterial genus and species belonged to Firmicutes, Actinobacteria, Proteobacteria, and Bacteroidetes were isolated from sow and weaned pigs. When comparing culture-dependent and culture-independent analyses, 191 bacterial species and 2 archaeal bacterial species were detected through culture-independent analysis, and a total of 23 bacteria were isolated through a culture-dependent approach, of which 65% were not detected by metagenomics. In conclusion, culturomics and metagenomics should be properly combined to fully understand the intestinal microbiota, and livestock-derived microbial resources should be informed by culturomic approaches to understand and utilize the mechanism of host-microbe interactions.

• 공연문화연구
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• 제41호
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• pp.39-67
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• 2020

그동안 극예술연구회의 제10회 공연작에 대한 연구는 거의 시행되지 않았다. 특히 이서향의 <어머니>는 거의 연구되지 않았으며, 기껏해야 제10회 공연작 중 하나였다는 사실 정도만 기록되곤 했다. <어머니>의 희곡이 남아 있고, 제10회 공연이 상당한 의의를 지닌 정기공연이었음에도 불구하고, 이러한 연구 실태는 대단히 열악한 것이 아닐 수 없다. 그것은 단막극 <어머니>가 드러내는 공연사적 가치와 의미를 정밀하게 고찰하지 못한 결과로 보인다. 이러한 문제를 극복하기 위해서는 <어머니>의 공연 정황을 살펴보고, 당시 극예술연구회의 상황을 조사할 필요가 있다. 그리고 <어머니>의 선정 이유, 실제 무대 공연 현황, 연극적 요소, 무대 디자인, 관련 평가를 두루 살펴보아야 한다. 이 연구는 이러한 <어머니>의 공연 정황을 점검하고 그 의의를 살피는 데에 초점을 두었으며, 그 결과를 통해 극예술연구회의 한 맥락으로서 여성 연극의 흐름에 대해 논구하고자 한다.

• Parasites, Hosts and Diseases
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• 제61권1호
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• pp.24-32
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• 2023

Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1), encoded by the polymorphic var multigene family, is a highly polymorphic antigen that plays a crucial role in the pathology of malaria. The contribution of the genetic diversity of var toward the immune escape of P. falciparum has not yet been fully elucidated. This study aimed to characterize the diversity of var repertoires by screening P. falciparum Duffy-binding-like α domain (PfDBLα) among field isolates from central Myanmar. Genetic analysis revealed that the D-H segments of var in Myanmar populations have an extensive polymorphic repertoire, with high numbers of unique sequence types in each individual. However, var genes from the global population, including Myanmar, shared close genetic lineages regardless of their geographic origins, indicating that they have not undergone rapid evolutionary changes.