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Alternative Immunossays

  • Barnard, G.J.R.;Kim, J.B.;Collins, W.P.
    • Korean Journal of Animal Reproduction
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    • v.9 no.2
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    • pp.133-139
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    • 1985
  • An immunoassay may be defined as an analytical procedure involving the competitive reaction between a limiting concentration of specific antibody and two populations of antigen, one of which is labelled or immobillized. The advent of immunoassay has revolutionised our knowledge of reproductive physiology and the practice of veterinary and clinical medicine. Radioimmunoassay (RIA) was the first of these methods to be developed, which meausred the analyte with good sensitivity, accuracy and precision (1,2). The essential components of RIA are:-(i) a limited concentration of antibodies, (ii) a reference preparation, and (iii) an antigen labelled with a radioisotope (usually tritium or iodine-125). Most procedures invelove isolating the antibody-bound fraction and measuring the amount of labelled antigen. Good facilities are available for scintilltion counting, data reduction nd statistical analysis. RIA is undergoing refinement through:-(i) the introduction of new techniques to separate the antibody-bound and free fractions which minimize the misclassification of labelled antigen into these compartments, and the amount of non-specfic binding. (3), (ii) the development of non-extration for the measurement of haptens (4), (iii) the determination of a, pp.rent free (i.e. non-protein bound) analytes (5), and (iv) the use of monoclonal antibodies(6). In 1968, Miles and Hales introduced in important new type of immunoassay which they termed immunora-diometric assay (IRMA) based on t도 use of isotopically labelled specific antibodies(7) in a move from limited to excess reagent systems. The concept of two-site IRMAs (with a capture antibody on a solid-phase, and a second labelled antibody to a different antigenic determinant of the analyte) has enabled the development of more sensitive and less-time consuming methods for the measurement of protein hormones ovar wide concentration of analyte (8). The increasing use of isotopic methos for diverse a, pp.ications has exposed several problems. For example, the radioactive half-life and radiolysis of the labelled reagent limits assay sensitivity and imposes a time limit on the usefulness of a kit. In addition, the potential health hazards associated with the use and disposal of radioactive cmpounds and the solvents and photofluors necessary for liquid scientillation counting are incompatable with the development of extra-laboratory tests. To date, the most practical alternative labels to radioisotopes, for the measurement of analytes in a concentration > 1 ng/ml, are erythrocytes, polystyrene particiles, gold sols, dyes and enzymes or cofactors with a visual or colorimetric end-point(9). Increased sensitivity to<1 pg/ml may be obtained with fluorescent and chemiluminescent labels, or enzymes with a fluorometric, chemiluminometric or bioluminometric end-point. The sensitivity of any immunoassay or immunometric assay depends on the affinity of the antibody-antigen reaction, the specific activity of the label, the precision with which the reagents are manipulated and the nonspecific background signal (10). The sensitivity of a limited reagent system for the measurement of haptens or proteins is mainly dependent upon the affinity of the antibodies and the smalleest amount of reagent that may be manipulated. Consequently, it is difficult in practice to improve on the sensitivity obtained with iodine-125 as the label. Conversely, with excess reagent systems for the measurement of proteins it is theoretically possible to increase assay sensitivity at least 1000 fold with alternative luminescent labels. To date, a 10-fold improvement has been achieved, and attempts are being made to reduce the influence of other variables on the specific signal from the immunoreaction.

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Measurement and Comparison of Emotions Felt by Each Type of Hanbok (한복 유형별로 느껴지는 감성의 측정과 비교)

  • Eun-Jung Park;Sang-Hoon Jeong;Jong-Hwan Seo
    • Science of Emotion and Sensibility
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    • v.25 no.4
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    • pp.33-44
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    • 2022
  • Rental services have recently come to be provided in which people can experience traditional Korean culture in the form of Hanok villages, allowing everyone can easily rent and wear hanbok, a traditional Korean garment, regardless of gender and age. Users of hanbok rental services share photos of themselves wearing hanbok on social media, contributing to the increasing popularity of hanbok experiences. However, the trend of wearing hanbok has no become established in people's daily lives, apart from the specific places that offer hanbok experiences. To promote hanbok as everyday clothes, hanbok design should be developed to provide wearers with both convenience and emotional satisfaction. Using 28 emotion words that express consumers' emotions toward hanbok that were extracted from previous studies, this study measured consumers' emotions toward different types of hanbok with on a seven-point Likert scale. Emotion words and categories that obtained scores of five points or above, signifying a rating of "felt fairly" or higher in relation to specific hanbok types were extracted. This study also examined differences in the average scores for emotional categories according to hanbok types and gender. The results indicated that only average scores for the "favorable feeling" category showed a significant difference between men and women. Finally, differences in the average scores for emotional categories were examined by classifying hanbok types: traditional (e.g., baenaet-jeogori, saekdong-jeogori, traditional hanbok for adults, and traditional wedding clothes) and modern (e.g., daily hanbok for children, for women, and for men). The results indicated significant differences between traditional and modern hanbok in six emotional categories (i.e., the cheerful, esthetic, harmonious, fresh, favorable, and stable feeling). This study derived analytic results for terms related to emotions that hanbok wearers feel according to types of hanbok. The findings can be used by hanbok retailers and rental services to provide consumers with greater emotional satisfaction.

An Estimation on the Applicability of Hollow FRP Soil Nailing System (중공식 FRP쏘일네일링 시스템의 적용성 평가)

  • Lee, Hyuk-Jin;Koh, Hyung-Seon;Han, Yong-Hee;Kim, Hong-Taek
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.26 no.6C
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    • pp.385-393
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    • 2006
  • Soil nailing is a reinforcement method used for stabilizing excavated walls or slopes. Due to its much advantages such as ease of construction and economical efficiency, use of soil nailing is increased. However, the soil nail has much disadvantages for use in urban area. The soil nail needs to be installed inevitably beyond private land boundary, which causes rent for use. For this reason, removable soil nailing system was developed. However, the removal rate of this system is just about 50¢¦70%. To resolve this problem, the Fiber Reinforced Plastic (FRP) soil nailing system which does not need to be removed and allows for the installation beyond private land, is developed. In this paper, through theoretical and experimental studies in laboratory and field such as prototype tests, pullout tests, we evaluate the stability and behavior characteristics of the FRP soil nailing system. And, numerical analyses using FLAC2D were performed with respect to various soil conditions, where prototype test for excavation wall and pullout tests were carried out. As a result of this study, the FRP soil nailing systems show similar behavior characteristics with those of removable soil nailing system. Finally, considering the serviceability and mechanical stability of FRP soil nailing systems, it is enough to be used as a good alternative of general soil nailing system.

Low-income Female Single-Parent Families' Experience of Economic Burden and Its Improvement by COVID-19 (코로나19(COVID-19)로 인한 저소득 여성한부모가족의 경제적 부담경험과 개선방안)

  • Sung, Jung Hyeun
    • The Journal of the Korea Contents Association
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    • v.22 no.8
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    • pp.527-541
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    • 2022
  • The purpose of this study is to explore the economic hardship experiences of low-income single-parent families by COVID-19 to find ways to improve after COVID-19. To this end, 10 single parents with children under the secondary school age were recruited through snowball sampling method and in-depth interviews with economic activities, income, expenditure, and economic life. As a result of the study, participants suffered from unemployment and the disappearance of extra income activities in the early stages of COVID-19, additional expenses incurred by children's food and increased utility bills. In addition, it was recognized that COVID-19 has come to be more difficult and unequal to single-parent families of low-income women. It was found that the temporary living difficulties were resolved with disaster support funds, and it was helpful in the short term by spending according to priorities such as overdue monthly rent, food expenses, children's academy expenses, and purchasing home appliances. However, it was recognized that disaster support funds were inappropriate for customized disaster support and long-term and selective support was necessary. In the conclusion, to improve economic problems after COVID-19, it proposed measures to improve social welfare policies and practices

Open Innovation in Car-Sharing Industry: Focusing on the Cooperation Case between Gongcar and Rental Car Company (카셰어링 산업의 개방형 혁신: (주)공카와 렌터카 업체간 개방형 혁신 사례를 중심으로)

  • Kiyeon Hwang;Jaehong Park;Youngwoo Sohn;Woosung Nam;Yeonhwa Cho
    • Asia-Pacific Journal of Business Venturing and Entrepreneurship
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    • v.19 no.1
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    • pp.93-105
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    • 2024
  • Car-sharing is a representative model of the sharing economy, and it is a service that rents or uses a car for the necessary time without owning a car. This industry is growing due to various factors such as technological advances, increasing awareness of environmental protection, and increasing demand for solving traffic congestion problems in cities. Accordingly, there is a need for a strategic approach for companies providing car-sharing services to respond quickly to market changes in order to expand market share and differentiate services. Accordingly, this study conducted a case study on open innovation activities between Gongcar and existing rental car companies, focusing on the research question "What effects do open innovation activities between car-sharing companies and existing rental car companies cause?" As a result of the study, it was confirmed that Gongcar have (1) the ability to actively respond to market fluctuations by establishing a flexible vehicle supply chain based on demand, (2) have significantly reduced growth capital expenditure (Growth Capex), and both cafe and rental car companies have (3) performed successful open innovation by improving key KPI indicators and recording financial performance. This study reveals how open innovation acts as a key business growth engine in the car-sharing industry, and its significance is found in that it empirically confirmed the successful implementation conditions of open innovation based on resource dependence theory.

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Spatial and temporal trends in food security during the COVID-19 pandemic in Asia Pacific countries: India, Indonesia, Myanmar, and Vietnam

  • Yunhee Kang;Indira Prihartono;Sanghyo Kim;Subin Kim;Soomin Lee;Randall Spadoni;John McCormack;Erica Wetzler
    • Nutrition Research and Practice
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    • v.18 no.1
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    • pp.149-164
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    • 2024
  • BACKGROUND/OBJECTIVES: The economic recession caused by the coronavirus disease 2019 pandemic disproportionately affected poor and vulnerable populations globally. Better uunderstanding of vulnerability to shocks in food supply and demand in the Asia Pacific region is needed. SUBJECTS/METHODS: Using secondary data from rapid assessment surveys during the pandemic response (n = 10,420 in mid-2020; n = 6,004 in mid-2021) in India, Indonesia, Myanmar, and Vietnam, this study examined the risk factors for reported income reduction or job loss in mid-2021 and the temporal trend in food security status (household food availability, and market availability and affordability of essential items) from mid-2020 to mid-2021. RESULTS: The proportion of job loss/reduced household income was highest in India (60.4%) and lowest in Indonesia (39.0%). Urban residence (odds ratio [OR] range, 2.20-4.11; countries with significant results only), female respondents (OR range, 1.40-1.69), engagement in daily waged labor (OR range, 1.54-1.68), and running a small trade/business (OR range, 1.66-2.71) were significantly associated with income reduction or job loss in three out of 4 countries (all P < 0.05). Food stock availability increased significantly in 2021 compared to 2020 in all four countries (OR range, 1.91-4.45) (all P < 0.05). Availability of all essential items at markets increased in India (OR range, 1.45-3.99) but decreased for basic foods, hygiene items, and medicine in Vietnam (OR range, 0.81-0.86) in 2021 compared to 2020 (all P < 0.05). In 2021, the affordability of all essential items significantly improved in India (OR range, 1.18-3.49) while the affordability of rent, health care, and loans deteriorated in Indonesia (OR range, 0.23-0.71) when compared to 2020 (all P < 0.05). CONCLUSIONS: Long-term social protection programs need to be carefully designed and implemented to address food insecurity among vulnerable groups, considering each country's market conditions, consumer food purchasing behaviors, and financial support capacity.

A Study on Titanium Miniscrew as Orthodontic Anchorage : An experimental investigation in dogs (성견에서 교정적 고정원으로서의 티타늄 미니스크류에 대한 연구)

  • Yoon, Byung-Soo;Choi, Byung-Ho;Lee, Won-You;Kim, Kyoung-Nam;Shim, Hyung-Bo;Park, Jin-Hyung
    • The korean journal of orthodontics
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    • v.31 no.5 s.88
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    • pp.517-523
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    • 2001
  • Titanium miniscrews we being used increasingly as an anchorage for tooth movement, because they ate easy to place and to remove, increase the number of sites available, give minimum strain to patients regarding surgical procedures, and offer uneventful healing alter removal. The use of titanium miniscrews as an orthodontic anchorage has been reported in clinical case reports, but clinicians have experienced screw loosening when using such screws.' To our knowledge, there are no published reports evaluating the stability of miniscrews. Information about the length of miniscrews used in relation to the location is of some importance, as stability will vary depending on bone duality The purpose of this study was to evaluate a variety of Lengths of miniscrews (dimeter: 2mm) which were inserted in maxilla or mandible and to demonstrate in a dog model which miniscrew provides fundamental stability in the jaws. 10 mm long miniscrews in the maxilla and 8mm long: miniscrews in the mandible showed no clinical mobility and retained their position throughout an 8 weeks force (200g) application. The mucosal condition around the screws was healthy in cases in which miniserews were inserted in the alveolar bone between the roots and the head of the screws emerged into the attached gingiva. When the force application was terminated, radiographic analysis revealed neither rent resorption not periodontal pathology around the miniscrews that remained stable during the entire treatment period. This study suggests that if titanium miniscrews with adequate length are properly used depending on the location, they provide sufficient stability for orthodontic anchorage.

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Review of Family Planning / Health Integration Efforts and Evaluation Results in Korea (가족계획과 보건사업의 통합시도 및 평가결과)

  • Bang, Sook
    • Korea journal of population studies
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    • v.10 no.2
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    • pp.58-81
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    • 1987
  • The purpose of this study is to examine major factors that affect patterns of living arrangements of older Korean noncitizens in the United States using the 1990 8% Public Use Microdata Sample(PUMS). In order to do so, I analyzed the effects of four factors including acculturation(English proficiency and Age at migration), economic circumstances(Personal Income and Supplemental Security Income: SSI receipt), health status(Mobility and Personal care limitation), and the ethnic community. The results show that acculturation and economic circumstances play an important role in determining the patterns of living arrangements among older Korean aliens, whereas the health status and the ethnic community do not show consistent effects. Regardless of marital status, the impact of age at migration is positively associated with solitary living of older Korean noncitizens, though, the effect of English proficiency show the opposite direction in accordance with marital status. That is, among nonmarried elders, those who cannot speak English are more likely to live alone. The case is reverse for married elders. The impact of economic feasibility is also positively associated with independent living arrangements. Those who receive SSI are much more likely to live apart from family, and elders whose annual personal income between $5,001 and $10,000 have the greatest propensity of solitary living-a large proportion of their income source is SSI. Individuals who receive SSI are also qualified to collect Medicaid, food stamps, rent subsidies, and other welfare benefits. In a sense, the economic feasibility provided by welfare benefits is the key determinant of independent living of older Korean noncitizens. Therefore, the recent welfare reform which denies legal aliens welfare benefits such as SSI and food stamps will severly affect the present living arrangements of older Korean aliens, and give economic burden to their family member The findings also show that there are significant differences within the elderly Korean Americans in terms of demographics, income, fertility, health status, and patterns of living arrangements by U.S. citizenship status. In particular, after controlling for age at migration as a proxy for acculturation, there is a statistically significant variation in living arrangements between elderly Korean noncitizens and naturalized citizens. For both theoretical and methodological reasons, future research on minority aging needs to investigate the concept of U.S. citizenship status for its impact on patterns of living arrangements among the minority elderly.

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Rice Cultivation and Demographi Development in Korea : 1429-1918 (조선시대(朝鮮時代) 도작농업(稻作農業)의 발전(發展)과 인구증가(人口增加))

  • Lee, Ho Chol
    • Current Research on Agriculture and Life Sciences
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    • v.7
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    • pp.201-219
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    • 1989
  • Rice culture in Korea has a long history ranging over two thousand years. In the agriculture economy of pre-mordern Korea, however, its importantce was not as great as generally assumed. In fact, rice culture reached full development only after the 1920s when the Japanese colonial government carried out its drive to increase rice production in the Korea peninsula. It was not until the mid-1930s that rice became the staple in Korean diet. This can be attributed to two factors : (1) a mountainous topography that provides little irrigated fields and (2) a climate characterized by droughts in spring and heavy precipitation in summer. The present paper attempts to answer some of these questions. Specifically it will focus on these : Did the development of rice culture actually result in population growth? What are the salient features of agricultural develdpment and population grow in traditional Korea? Does the case of Korea conform the prevailing generalization about the agriculture in East Asia? I have discussed the development of rice culture and population growth in the Chos$\breve{o}$n dynasty, focusing on the relation between the rapid spread of transplanting and the rapid growth of population from the seventeenth to the nineteenth century. Here are my conclusions. (1) The spread of transplanting and other technological innovationsc contributed to the rapid growth of population in this period. However, we should also note that the impact of rice culture on population growth was rather limited, for rice culture was not the mainstay of agricultural economy in pre-modern Korea. Indeed we should consider the influence of dry field cropsn population growth. Nevertheless, it is obvious that the proliferation of rice culture was a factor crucial to population growth and regional concentration. (2) How should we characterize the spread of rice culture in the whole period? Evidently rice culture spread from less then 20% of cultivated fields in the fifteenth century to about 36% of them in the early twentieth century. Although rice as a single crop outweighed other crops, rice culture was more then counter-balanced by dry field crops as a whole, due to Korea's unique climate and geography. Thus what we have here in not a typical case of competition between rice culture and day field culture. Besides, the spread of rice culture in the seventeenth and eighteenth centuries accomplished by technological innovations that overcame severe springtime drought, rather than extensive irrigation. Althougt irrigarion facilities did proliferate to some extent, this was achieved by local landlords and peasants rather than the state. This fact contradicts the classical thesis that the productivity of rice culture increased through the state management of irrigation and that this in turn determined the type of society. (3) We should further study other aspects of the transition from the stable population and production struture in the fifteenth and sixteenth centuries to the rapid population growth and excessive density of population thereafter. We should note that there were continuing efforts to reclaim the land in order to solve the severe shortage of land. Changes also took place in the agricultural production relations. The increase in land producrivity developed tenancy based on rent in kind, and this in turn increased the independence of tenants from their landlords. There were changes in family relations-such as the shift to primogeniture as an effort to prevent progressive division of property among multiplying offspring. The rapid population growth also produced a great mass of propertyless farm laborers. These changes had much to do with the disintegration of traditional social institutions and political structure toward the end of the Chos$\breve{o}$n dynasty.

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Effects of Health Perception, Self-efficacy and Social Support of College Students on the Health Promotion Behaviors (대학생의 건강지각, 자기효능감, 사회적 지지가 건강증진행위에 미치는 영향)

  • Woo, Bok Jin;Lee, Hye Kyung
    • Journal of the Korean Applied Science and Technology
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    • v.36 no.4
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    • pp.1290-1302
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    • 2019
  • This study is a descriptive research with a purpose of examining the effects of health perception, self-efficacy and social support of college students on the health promotion behaviors. The subjects of this study have targeted 196 college students, where the data collection was made from September 10, 2018 to October 5, 2018. As a result of analyzing the differences in health promoting behaviors according to general characteristics of college students, there was a statistically significant difference according to the residence type(F=8.56, p<.001), subjective health status(F=7.23, p=.001), university life satisfaction(F=8.50, p<.001), exercise status(t=6.62, p<.001), and number of breakfasts per week(F=14.13, p<.001). For the correlation between college students' health perception, self-efficacy, social support, and health promotion behaviors, health promotion behavior and health perception(r=44, p<.001), self-efficacy(r=.57, p<.001), and social support(r=.49, p<.001) has shown a significant positive correlation between them. As a result of conducting multiple regression analysis by setting general characteristics, health perception, self-efficacy and social support as independent variables in order to analyze the factors affecting health promotion behaviors of college students, The variables that have significant explanatory power on health promoting behaviors(F=34.921, p<.001) were self-efficacy, social support, number of breakfasts per week(more than 5times), whether or not to exercise, number of breakfasts per week(2 to 4times), rent and lodging for residential type, and for grade, seniors. The explanatory power of these factors on health promotion behaviors was a total of 56.9%. Conclusively, In order to Improving health promotion behavior requires the development and intervention of personalized health promotion programs for each generation that consider self-efficacy and social support and emphasize the importance of proper exercise and proper eating habits.