• Molecules and Cells
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• v.37 no.1
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• pp.81-87
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• 2014

Chronic pressure-overload cardiac hypertrophy is associated with an increased risk of morbidity/mortality, largely due to maladaptive remodeling and dilatation that progresses to dilated cardiomyopathy. Alternative splicing is an important biological mechanism that generates proteomic complexity and diversity. The recent development of next-generation RNA sequencing has improved our understanding of the qualitative signatures associated with alternative splicing in various biological conditions. However, the role of alternative splicing in cardiac hypertrophy is yet unknown. The present study employed RNA-Seq and a bioinformatic approach to detect the RNA splicing regulatory elements involved in alternative splicing during pressure-overload cardiac hypertrophy. We found GC-rich exonic motifs that regulate intron retention in 5' UTRs and AT-rich exonic motifs that are involved in exclusion of the AT-rich elements that cause mRNA instability in 3' UTRs. We also identified motifs in the intronic regions involved in exon exclusion and inclusion, which predicted splicing factors that bind to these motifs. We found, through Western blotting, that the expression levels of three splicing factors, ESRP1, PTB and SF2/ASF, were significantly altered during cardiac hypertrophy. Collectively, the present results suggest that chronic pressure-overload hypertrophy is closely associated with distinct alternative splicing due to altered expression of splicing factors.

• Molecules and Cells
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• v.39 no.5
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• pp.395-402
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• 2016

Identifying Hoxc8 target genes is at the crux of understanding the Hoxc8-mediated regulatory networks underlying its roles during development. However, identification of these genes remains difficult due to intrinsic factors of Hoxc8, such as low DNA binding specificity, context-dependent regulation, and unknown cofactors. Therefore, as an alternative, the present study attempted to test whether the roles of Hoxc8 could be inferred by simply analyzing genes frequently coexpressed with Hoxc8, and whether these genes include putative target genes. Using archived gene expression datasets in which Hoxc8 was differentially expressed, we identified a total of 567 genes that were positively coexpressed with Hoxc8 in at least four out of eight datasets. Among these, 23 genes were coexpressed in six datasets. Gene sets associated with extracellular matrix and cell adhesion were most significantly enriched, followed by gene sets for skeletal system development, morphogenesis, cell motility, and transcriptional regulation. In particular, transcriptional regulators, including paralogs of Hoxc8, known Hox co-factors, and transcriptional remodeling factors were enriched. We randomly selected Adam19, Ptpn13, Prkd1, Tgfbi, and Aldh1a3, and validated their coexpression in mouse embryonic tissues and cell lines following $TGF-{\beta}2$ treatment or ectopic Hoxc8 expression. Except for Aldh1a3, all genes showed concordant expression with that of Hoxc8, suggesting that the coexpressed genes might include direct or indirect target genes. Collectively, we suggest that the coexpressed genes provide a resource for constructing Hoxc8-mediated regulatory networks.

• Journal of Korean Society for Atmospheric Environment
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• v.28 no.4
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• pp.435-445
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• 2012

The aim of this study is to understand the characteristics of volatile oranic compounds (VOCs) and provide information about the present Indoor Air Quality (IAQ) at residential apartments. All samples were collected in 60-min interval using the tenax absorption trap between May, 2011 and February, 2012. And the effects of environmental factors such as temperature, humidity and construction characteristics were analyzed in relation to the measured concentrations. The results of this study showed that the mean concentration of VOCs was lower than the Ministry of the Environment's standards for maintenance of indoor air quality. The correlation analysis showed that ethylbenzene and xylene (r=0.916, p<0.01), toluene and ehtylbenzene (r=0.810, p<0.01), toluene and xylene (r=0.803, p<0.01) and toluene and styrene (r=0.588, p<0.01) were significant. The result of regression analysis was found that the influenece factors associated with the concentration of VOCs were the age and location of the apartment, remodeling, the temperature and the season.

• Journal of The Korea Institute of Healthcare Architecture
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• v.25 no.4
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• pp.27-35
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• 2019

Purpose: As the improvement in quality of medical environment and the spatial consideration for the prevention of infection in the hospital have become increasingly important in the recent years, specific and practical planning for air conditioning room's area has become a major issue accordingly. However, the air conditioning room tends to be discussed focusing on technical factors such as air conditioning systems that are irrelevant to building plans, while discussions of most departments related to the medical functions have actively taken placed. Therefore, this study aims to investigate the factors influencing the planning for the air conditioning room area, and through subsequent analysis of the area of the air conditioning room, to propose a way to improve effective planning for the air conditioning room area. Methods: This study examines the case hospitals that fall into two types: each floor supply system and concentrated supply system, and compares and analyzes the air conditioning room area-related factors and the characteristics of changes in the interior of air conditioning room before and after remodeling through air conditioning floor plan of those case hospitals. Results: The air conditioning room can be classified into the net area of the functional space such as duct passages, and the public area such as aisle space, and of those the public area is an important factor in calculating the area of the air conditioning room. The public area of the air-conditioning room should not be planned considering only the spaces for mobile passage or maintenance activities, but should be planned taking into account internal changes such as expansion and replacement of equipment in the future from the beginning. Implications: When planning a medical facility, it is used as basic data for the planning of the air conditioning room area, which is a significant fixed factor in the initial setting.

• Proceedings of the Korean Institute of Interior Design Conference
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• 2006.05a
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• pp.268-271
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• 2006

The purpose of this study was to identify the influence of living factors on TVOC(Total Volatile Organic Compounds) concentrations for newly-built apartments. The field measurements of TVOC concentrations were made totally 30 times(5 times per one subject house) in six apartment units in which residents recently moved. Those apartments authorized its business approval before May 2004, the Act of Indoor Air auality Management for multi-use facilities. The findings were as follows: The TVOC concentrations of the measured subject apartments ranged from about 1/10 of the recommended standard for multi-use facilities($400{\mu}g/m^3$) to up to 90 times as high as the standard. Since then, the Recommended Standard of Indoor Air Quality Management for newly-built apartment house was announced in December 2005. In accordance with this standard($2390{\mu}g/m^3$) it ranged from about 1/100 to up to 15 times as high as the standard. The subject house whose TVOC concentrations reduced below the recommendation standard in the shortest period had the largest amount of ventilation (all the windows were open for ventilation in the past three months) among ail measured houses. The reason of another house whose TVOC concentrations were much higher than the rest was fronted with new furniture in the room. It's recommended that they should open all the windows for at least three months for ventilation in newly-built houses, and it would be better to avoid remodeling than needs be.

• Fire Science and Engineering
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• v.32 no.4
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• pp.86-94
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• 2018

A sports center fire in Jecheon caused 29 deaths and 40 injuries. This study focused only on the structural factors of the building and equipment to investigate the causes of casualties based on the fire investigation results at the sports center. The structural factors of the building and equipment were a piloti-type parking lot, lack of a fire compartment between the piloti-type parking lot and lobby, lack of an installed sprinkler system, lack of an installed fire door in the main stairs on the $1^{st}$ floor, lack of an installed fire water tank on the rooftop, an installed pocket fire door in the main entrance on the $2^{nd}$ floor, poor fire compartments in an EPS space and a pipe pit and on each floor, a leak in the joint of a drain pipe, plywood installed in the hoistway of the freight elevator and interior wall, illegal remodeling of a closed rooftop structure, which cannot discharge smoke and heat, installed styrofoam insulation in the inside of the parking lot ceiling, an installed tempered glass window, which cannot be opened in the ladies bathroom on the $2^{nd}$ floor, and a finished dryvit exterior wall.

• Korean Journal of Construction Engineering and Management
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• v.20 no.6
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• pp.132-141
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• 2019

1990s, Construction market continued to grow in South Korea exceeding 20% of GDP. In 2015, more than 30 years of old multi-family dwellings amounted to 4.93 million and it continues to increase until 2019. In Particular, the share of Public rental Housing accounts for 90.6% of South Korea's Total aging housing. Before checking the feasibility of Construction Project Management such as Renovation and Reconstruction, Checklists were presented through price impact factor analysis and analysis of housing construction system(Regulation). Based on 32 Price Influencing factors and 20 Indicators based on the housing construction system The Construction project management seems appropriate. As a result, the 22 complexes were found to be suitable for remodeling. This study is meaningful enough to be used for Domestic rental Housing construction and Construction project management and to suggest specificity. Future Research is needed to Quantify the checklist.

• The Journal of the Korean dental association
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• v.54 no.5
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• pp.348-355
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• 2016

Ideal autogenous or allogenic bone graft materials should provide 1) stabilization of blood clot, 2) scaffolds for cellular proliferation and differentiation, 3) release of osteogenic growth factors, 4) appropriate resorption profile for remodeling of new bone. Teeth, especially dentin, mostly contain hydroxyapatite and type I collagen which are similar to bone, and could be valuable graft material. Clinically teeth are used as calcined or demineralized forms. Demineralized form of dentin can be more effective as a graft material. But a conventional decalcification method takes time and long treatment time may give negative effects to various osteogenic proteins in dentin. Author used a new clinical method to prepare autogenous teeth, which could be grafted into the removal defects immediately after extraction using vacuum ultrasonic system. The process could be finished within two hours regardless of the form (powder, chip or block). Teeth were processed to graft materials in block, chip, or powder types immediately after extraction. It took 120 minutes to prepare block types and 40 minutes to prepare powder. Clinical cases did not show any adverse response and the healing was favorable. Rapid preparation of autogenous teeth with the vacuum ultrasonic system could make the immediate one-day extraction and graft possible.

• BMB Reports
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• v.42 no.4
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• pp.227-231
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• 2009

The eukaryotic genome forms a chromatin structure that contains repeating nucleosome structures. Nucleosome packaging is regulated by chromatin remodeling factors such as histone chaperones. The Saccharomyces cerevisiae H3/H4 histone chaperones, CAF-1 and Asf1, regulate DNA replication and chromatin assembly. CAF-1 function is largely restricted to non-transcriptional processes in heterochromatin, whereas Asf1 regulates transcription together with another H3/H4 chaperone, HIR. This study examined the role of the yeast H3/H4 histone chaperones, Asf1, HIR, and CAF-1 in chromatin dynamics during transcription. Unexpectedly, CAF-1 was recruited to the actively transcribed region in a similar way to HIR and Asf1. In addition, the three histone chaperones genetically interacted with Set2-dependent H3 K36 methylation. Similar to histone chaperones, Set2 was required for tolerance to excess histone H3 but not to excess H2A, suggesting that CAF-1, Asf1, HIR, and Set2 function in a related pathway and target chromatin during transcription.

• Clinical and Experimental Pediatrics
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• v.57 no.11
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• pp.472-478
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• 2014

Kawasaki disease (KD), an acute vasculitis that primarily affects young children, is the most common acquired paediatric cardiovascular disease in developed countries. While sequelae of arterial inflammation in the acute phase of KD are well documented, its late effects on vascular health are increasingly unveiled. Late vascular dysfunction is characterized by structural alterations and functional impairment in term of arterial stiffening and endothelial dysfunction and shown to involve both coronary and systemic arteries. Further evidence suggests that continuous low grade inflammation and ongoing active remodeling of coronary arterial lesions occur late after acute illness and may play a role in structural and functional alterations of the arteries. Potential importance of genetic modulation on vascular health late after KD is implicated by associations between mannose binding lectin and inflammatory gene polymorphisms with severity of peripheral arterial stiffening and carotid intima-media thickening. The changes in cholesterol and lipoproteins levels late after KD further appear similar to those proposed to be atherogenic. While data on adverse vascular health are less controversial in patients with persistent or regressed coronary arterial aneurysms, data appear conflicting in individuals with no coronary arterial involvements or only transient coronary ectasia. Notwithstanding, concerns have been raised with regard to predisposition of KD in childhood to accelerated atherosclerosis in adulthood. Until further evidence-based data are available, however, it remains important to assess and monitor cardiovascular risk factors and to promote cardiovascular health in children with a history of KD in the long term.