• 한국산림과학회지
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• 제40권1호
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• pp.43-50
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• 1978

본(本) 연구(硏究)는 천연식용색소(天然食用色素)와 고급주(高級酒)및 천연과실(天然果實) 음료(飮料) 개발(開發)의 중요자원(重要資源)이 되는 Elder berry의 다량증식(多量增殖)과 보급(普及)을 위(爲)하여 각처리별(各處理別)로 삽목실험(揷木實驗)을 실시(實施)한 결과(結果)다음과 같은 결과(結果)를 얻었다. 1. 삽목상(揷木床)은 온실내(溫實內)에 온도(溫度)와 습도(濕度)를 완전자동화(完全自動化) 할때에 100%의 활착(活着)을 높일 수 있었고 처리간(處理間)에는 1%의 높은 유의성(有意性)이 있었다. 2. 품종간(品種間) 비교실험(比較實驗)에 있어서는 평균(平均) 93.8%의 삽목활착율(揷木活着率)을 보였고 품종내(品種內)에는 유의성(有意性)이 없었다. 3. 삽수(揷穗)의 각기관별(各器管別) 실험(實驗)에서는 평균(平均)57.5%의 삽목활착율(揷木活着率)을 냈으며 휴안지(休眼枝) 2 마디를 쓰는 것이 가장 좋았으며 처리간(處理間)에는 1%의 유의성(有意性)을 보였다. 4. 휴안아(休眼芽)의 삽목(揷木)이 가능(可能)하여 17.66%의 활착(活着)이 되었다. 5. 조직(組織)의 분화별(分化別) 및 경화도(硬化度)에 따른 삽목활착(揷木活着)에는 1% 이상의 유의성(有意性)이 있었고 6月 15일경(日頃)의 반숙지(半熟枝)인 경우가 54%로서 가깅 좋은 결과(結果)를 얻었다.

• 한국어병학회지
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• 제31권1호
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• pp.35-39
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• 2018

Atypical furnculosis caused by atypical Aeromonas salmonicida, is an emerging problem of farming of rockfish (Sebastes schlegeli) in Korea. In this study, protection against atypical furunculosis was compared in rockfish vaccinated with A. salmonicida formalin killed cell (FKC) and adjuvant containing FKC. The formalin inactivated A. salmonicida vaccine provided a low protection of 20% and 10% relative percent survival (RPS) at 44 and 58 days post vaccination. However, addition of adjuvant (squalene and aluminum hydroxide) into inactivated A. salmonicida vaccine clearly enhanced the level of protection showing 70% and 50% RPS at 44 and 58 days post vaccination.

• 생물환경조절학회지
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• 제10권3호
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• pp.165-171
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• 2001

겨울철 시설내에서 정전 등으로 인하여 수시간 동안 작물체가 생육한계 온도로 경과시 오이묘에 나타나는 피해와 피해발생의 생리적 요인 및 피해 경감대책을 구명하기 위하여 저온처리 전후의 광환경 조건이 오이 묘의 생존율 및 생육반응에 미치는 영향을 조사하였다. 냉온처리(2$^{\circ}C$, 48시간)에 따른 오이묘의 생존율은 암기말인 07:00시 처리에서 30.6%이었고, 암기 개시기인 18:00시 처리에서는 92.2%로 높았다. 세포의 전해질 누출은 07:00시 처리에서(72시간) 57.1%, 18:00시 처리에서는 25.9%로 낮았다. 누출액중 무기이온 함량은 $K^{+}$ 이온이 가장 많았고, $Na^{+}$, $Mg^{+2}$ , $Ca^{+2}$ 순으로 적었다. 냉온처리전 야간에 광을 조사하면 생존율이 광 무처리에 비해 56% 더 높아졌으며, 광조사는 2시간 처리로 충분한 효과가 있었다. 광 조사에 의하여 생존율이 높아지는 것은 광합성에 의한 당함량의 증가 때문인 것으로 해석 되었다. 냉온처리(3$^{\circ}C$, 48시간) 기간중 광을 조사하면, 생존율이 43.3%로 낮아진 반면, 암조건에두면 92%로 높았다.

• 한국어병학회지
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• 제15권1호
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• pp.25-35
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• 2002

Effects of medicinal herb extract on nonspecific immune responses, hematology and disease resistance against Edwardsiella tarda in olive flounder, Paralichthys olivaceus were evaluated. Wormwood, Artemisia asiatica NAKAI and barrenwort, Epimedium koreanum NAKAI were mixed at a ratio of 7 : 3 (w/w) for 2-herbs extract and wormwood, barrenwort, Korean forsythia, Forsythia koreana NAKAI, chrysanthemum, Chrysanthemum zawadskii var. latilobum KITAMURA, peppermint, Mentha arvensis L. var, piperascens MALINV., great burnet, Snaguisorba afficinalis L., Lizard tail. Saururus chinensis BAILL., mulberry, Morus alba L., and star anise, Illicium varum HOOK, f, at the same weight for 9-herbs extract. Two-herbs of 9-herbs extract were prepared by heating after adding 10㎖ of distilled water per g of the herb mixtures. Fish (10.3$\pm$2.5g) were fed the experimental diets supplemented with the 2-herbs or 9-herbs extract at the different concentrations of 0%, 0.1%, 0.5% and 1% per kg diet for 12 weeks. Lysozyme and bactericidal activities of serum, and hematological characteristics were examined during experimental period. After feeding test period, all experimental groups were challenged with E. tarda. Lysozyme activity from the fish fed the diet supplemented with 0.1% or 0.5% of 2-herbs extract was significantly higher than the control. But there was no difference both in bactericidal activity and hematology among each group. Sixty seven % of relative percent survival values (RPS) in the group fed the diet supplemented with 0.1% of 2-herbs was higher than the other group and the control. These results suggest that supplenmentation of 0.1% of 2-herbs extract to a commercial diet may enhance disease resistance in olive flounder. Although both 0.1% and 0.5% 9-herbs extract did not improve non-specific immune reponses, they could enhance disease resistance of 53% RPS, respectively.

• Journal of Gastric Cancer
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• 제22권3호
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• pp.160-168
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• 2022

This study investigated the trends of gastric cancer in Korea by adding the latest updated gastric cancer data from 2019. Gastric cancer incidence between 1999 and 2019 was reviewed using data from the Korea Central Cancer Registry. The study period was divided into 3 periods: period I (1999-2005), period II (2006-2012), and period III (2013-2019). The incidence, mortality, tumor location, histology, stage, and treatment were analyzed. Between 1999 and 2019, 577,502 patients were newly diagnosed with gastric cancer in Korea, accounting for 33.2% of patients aged ≥ 70 years. The age-standardized incidence rate (per 100,000) significantly decreased from 2011 (43.0) to 2019 (29.6), with an annual percent change of -4.50. Additionally, the age-standardized mortality rate (per 100,000) markedly decreased from 1999 (23.9) to 2019 (6.7). The proportions of patients with cardia and fundus cancers remained consistent. The proportion of localized stage cases increased, while those of regional and distant stages decreased. The rate of surgical treatment increased in localized and regional stages from 2006 to 2019. The overall 5-year relative survival (5YRS) rate of gastric cancer (per 100,000) increased from 55.7% in period I to 77.0% in period III. From 2013 to 2019, the 5YRS rates of patients (per 100,000) who underwent surgical treatment were 100.6% and 70.5% in the localized and regional stages, respectively. The results of this study demonstrated several changes in the epidemiology of gastric cancer in Korea. This study provides information to help understand the current trends in gastric cancer in Korea.

• Asian Pacific Journal of Cancer Prevention
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• 제16권16호
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• pp.7295-7302
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• 2015

Background: Lung cancer has been a major health problem in developed countries for several decades, and has emerged recently as the leading cause of cancer death in many developing countries. The incidence of lung cancer appears to be increasing more rapidly in rural than in urban areas of China. This paper presents the trends of lung cancer incidence and survival derived from a 40-year population-based cancer monitoring program in a rural area, Qidong, China. Materials and Methods: The Qidong cancer registration data of 1972-2011 were used to calculate the crude rate, age-standardized rate by Chinese population (CASR) and by world population (WASR), birth cohort rates, and other descriptive features. Active and passive methods were used to construct the data set, with a deadline of the latest follow-up of April 30, 2012. Results: The total number of lung cancer cases was 15,340, accounting for 16.5% of all sites combined. The crude incidence rate, CASR and WASR of this cancer were 34.1, 15.7 and 25.4 per 100,000, respectively. Males had higher crude rates than females (49.7 vs 19.0). Rapidly increasing trends were found in annual percent change resulting in lung cancer being a number one cancer site after year 2010 in Qidong. Birth cohort analysis showed incidence rates have increased for all age groups over 24 years old. The 5 year observed survival rates were 3.55% in 1973-1977, 3.92 in 1983-1987, 3.69% in 1993-1997, and 6.32% in 2003-2007. Males experienced poorer survival than did females. Conclusions: Lung cancer has become a major cancer-related health problem in this rural area. The rapid increases in incidence likely result from an increased cigarette smoking rate and evolving environmental risk factors. Lung cancer survival, while showing some improvement in prognosis, still remains well below that observed in the developed areas of the world.

• 한국어병학회지
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• 제19권2호
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• pp.165-172
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• 2006

본 연구는 Streptococcus iniae 포르말린 불활화 백신을 냉장 보관한 후 보존 기간별로 넙치에 접종하여 면역 효능 및 안정성을 조사하였다. 그 결과 6, 12, 15개월 보존한 후에도 면역 효능의 감소가 확인되지 않아 백신으로서의 높은 안정성을 확인할 수 있었다. 또한, 1회 접종구에 비해 2회 접종구에서 높은 항체가가 유도되었으며 방어력도 증가하는 것으로 나타났다. 특히, 1회 접종만으로도 낮은 항체가에도 불구하고 60% 이상의 방어 효과가 나타남에 따라 항체 이외의 세포성 면역이 방어력 형성에서 중요한 역할을 하는 것으로 추정되었다.

• 수산해양교육연구
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• 제26권6호
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• pp.1193-1200
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• 2014

Edwardsiella tarda, Streptococcus iniae and S. parauberis are main bacterial pathogens in aquaculture farms of olive flounder, Paralichthys olivaceus. We have discussed the efficacy and safety of 3 type-combined vaccines (A: S. iniae 1mg + S. parauberis 1mg + E. tarda 1mg, B: S. iniae 1mg + S. parauberis 1mg + E. tarda 0.5mg, C: S. iniae 1.5mg + S. parauberis 1.5mg + E. tarda 1mg) through intraperitoneal injections in olive flounder. None of the vaccines gave rise to any signigicant side effects on histopathology and blood chemistry. The antibody titers and lysozyme activities of A type were higher than those of B, C and control. Four weeks after vaccination, RPS (relative percent survival rates) was 62.5~75% (A type), 50~66.7% (B type) and 55.6~62.5% (C type) respectively. As the results, the combined vaccines are possible to prevent edwardsiellosis and streptococcosis, and A type : S. iniae 1mg + S. parauberis 1mg + E. tarda 1mg, is the most effective out of them.

• 한국수산과학회지
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• 제54권5호
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• pp.652-659
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• 2021

Microorganism-derived compounds, such as peptidoglycan, lipoteichoic acid, and β-glucan were supplemented in the scuticociliate Miamiensis avidus (M. avidus) vaccine to verify the specify component contribution to the adjuvant effect. Vaccine was formulated with the inactivated M. avidus antigen (YS2, 4.44×10⁵ cells/fish) in combination with either peptidoglycan (10 ㎍ and 100 ㎍/fish), lipoteichoic acid (5 ㎍ and 50 ㎍/fish), or β-glucan (10 ㎍ and 100 ㎍/fish). Olive flounder injected with peptidoglycan supplemented vaccine (10 ㎍ and 100 ㎍/fish) exhibited significant protection, and the relative percent survival (RPS) was 55% and 65% at 4 weeks post vaccination (wpv), respectively, at the corresponding doses. The vaccine groups with added lipoteichoic acid (5 ㎍ and 50 ㎍/fish) exhibited RPS of 40% and 5%, respectively. Additionally, the group with added β-glucan (100 ㎍/fish) exhibited RPS of 35%, but no effect was observed in the group with added 10 ㎍/fish β-glucan. At 8 wpv, olive flounder injected with peptidoglycan and lipoteichoic acid supplemented vaccines exhibited protection with RPS range of 11/11% and 5/21%, respectively, at the respective doses. M. avidus vaccine containing 10 ㎍ and 100 ㎍/fish of β-glucan exhibited the RPS of 32% and 37%, respectively. Conclusively, peptidoglycan contributed in high protection of the M. avidus vaccine, and thus, it can be used as an effective adjuvant in the M. avidus vaccine.

• 수산해양교육연구
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• 제27권5호
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• pp.1508-1521
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• 2015

Vibrio harveyi is a pathogenic marine bacterium causing systemic symptoms resulting in mass mortalities in fishes and shrimps in aquaculture. Outer membrane proteins(OMPs) are related to the pathogenicity and thus good targets for diagnosis and vaccination for Gram negative bacteria. Recently vaccination strategies using the OMPs have been suggested to control vibriosis in several fish species. In this study, we have isolated V. harveyi from diseased marine fishes from different regions of Korea and investigated genetic variations of four OMP genes including OmpK, OmpU, OmpV and OmpW. Consequently, OmpK and U genes could be divided into 3 subgroups of type I, II, III and type A, B, C, respectively, without any correlation with geographical regions and species while OmpV and W were highly homologous. OmpW gene of V. harveyi FP4138 was fully sequenced and predicted the deduced amino acid sequence to form ${\beta}-barrel$ with hydrophobic channel. Indeed, the immunogenicity of recombinant OmpW produced in Escherichia coli was assessed by vaccinating flounder. As a result, the high antibody response with antibody titer of $4.2{\pm}0.7$ and protection with relative percent survival of 60% against artificial infection of V. harveyi were demonstrated. This result indicates that OmpW is a virulence related factor and it can be a vaccine candidate to prevent a high mortality caused by V. harveyi infection in olive flounder, Paralichthys olivaceus.