DOI QR코드

DOI QR Code

The Efficacy and Safety on Combination Vaccines : Edwardsiella tarda, Streptococcus iniae and S. parauberis, in Olive flounder, Paralichthys olivaceus

넙치, Paralichthys olivaceus에서 Edwardsiella tarda, Streptococcus iniae와 S. parauberis 혼합백신의 효능 및 안전성 연구

  • Received : 2014.07.28
  • Accepted : 2014.10.10
  • Published : 2014.12.31

Abstract

Edwardsiella tarda, Streptococcus iniae and S. parauberis are main bacterial pathogens in aquaculture farms of olive flounder, Paralichthys olivaceus. We have discussed the efficacy and safety of 3 type-combined vaccines (A: S. iniae 1mg + S. parauberis 1mg + E. tarda 1mg, B: S. iniae 1mg + S. parauberis 1mg + E. tarda 0.5mg, C: S. iniae 1.5mg + S. parauberis 1.5mg + E. tarda 1mg) through intraperitoneal injections in olive flounder. None of the vaccines gave rise to any signigicant side effects on histopathology and blood chemistry. The antibody titers and lysozyme activities of A type were higher than those of B, C and control. Four weeks after vaccination, RPS (relative percent survival rates) was 62.5~75% (A type), 50~66.7% (B type) and 55.6~62.5% (C type) respectively. As the results, the combined vaccines are possible to prevent edwardsiellosis and streptococcosis, and A type : S. iniae 1mg + S. parauberis 1mg + E. tarda 1mg, is the most effective out of them.

Keywords

References

  1. Chen, C.-Y. Gregory, A. W. and Paul, R. B.(2004). Comparative blood chemistry and histopathology of tilapia infected with Vibrio vulunificus or Streptococcus iniae or exposed to carbon tetrachloride, gentamicin, or copper sulfate. Aquaculture, 239, 421-443. https://doi.org/10.1016/j.aquaculture.2004.05.033
  2. Cho, Mi Young Kim, Myung Sug Choi, Hye Sung Park, Gyeong Hyun Kim, Jin Woo Park, Mi Seon and Park, Myoung Ae(2008). A statistical study on infectious diseases of cultured olive flounder, Paralichthys olivaceus in Korea, Journal of Fish Pathology, 21, 271-278.
  3. Grinde, B.(1989). Lysozyme from rainbow trout, Salmo gairdneri Richardon, as an antibacterial agent against fish pathogens, J. Fish. dis., 12, 95-104. https://doi.org/10.1111/j.1365-2761.1989.tb00281.x
  4. Jeong, Yong-Uk Kang, Chul-Young Kim, Min-Ju Heo, Moon-Soo Oh, Duck-Chul and Kang, Bong-Ho (2006). Characterization of Streptococcosis occurrence and molecular identification of the pathogens of cultured flounder in Jeju island, The Korean Journal of Microbiology, 42, 199-204.
  5. Kim, Jin Woo Park, Soo-Il and Chun, Seh-Kyu (1992). Purification and antibacterial effect of lysozyme from flounder, Paralichthys olivaceus, Journal of Fish Pathology, 5, 61-76.
  6. Lee, S. and Chong, Y.(2000). Laboratory methods in clinical pathology, Fourth edition, Yonsei University Press, Seoul, Korea, 223-230.
  7. Maita, M. Aoki, H. Yamagata, Y. Satoh, S. Okamoto, N. and Watanabe, T.(1998a). Plasma biochemistry and disease resistance in yellowtail fed a non-fish meal diet, Fish Pathology 33(2), 59-63. https://doi.org/10.3147/jsfp.33.59
  8. Maita, M. Satoh, K.-I. Fukusa, Y. and Lee, H.-K. (1998b). Correlation between plasma component levels of culture fish and resistance to bacterial infection, Fish Pathology 33(3), 129-133. https://doi.org/10.3147/jsfp.33.129
  9. SPSS Inc. SPSS Base 7.5 for Window, SPSS Inc., 444N(1997): Michigan Avenue, Chicago, IL,.
  10. Wedemeyer, G. A. and McLeay, D. J.(1981). Methods of determine the tolerance of fishes to environmental stressors. In Stress in Fish. pp. 247-275, Pickering, A. D. Academic Press, London.
  11. Wedemeyer, G. A., Barton, B. A. and McLeay, D. J.(1990): Stress and acclimation. In Methods for Fsih Biology, pp. 451-489, Scherck, C. B. and Moyle, P. B., Am. Fish. Soc., Bechesda, Maryland.
  12. Yanagisawa, T. and Hashimoto, K.(1984). Plasma albumins in elasmobranchs. Bulletin of the Japanese Society of Scientific Fisheries 50(6), 1083.

Cited by

  1. Effect of Olive Flounder, Paralichthys olivaceus, Immune Genes Stimulation by Molecular Adjuvant in Vitro Culture Condition vol.27, pp.5, 2015, https://doi.org/10.13000/JFMSE.2015.27.5.1470