Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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Effects of Medicinal Herb Extract on Non - specific Immune Responses , Hematology and Disease Resistance on Olive Flounder , Paralichthys olivaceus by Oral Administration

생약재 첨가 사료를 투여한 넙치( Paralichthys olivaceus )의 비특이적 면역반응, 혈액성분 및 항병력 효과

  • Jung, Sung-Hee (Pathology Division, National Fisheries Research & Development Institute) ;
  • Lee, Joo-Seok (Pathology Division, National Fisheries Research & Development Institute) ;
  • Han, Hyoung-Kyun (Koje Marine Hatchery, South Sea Fisheries Research Institute, NFRDI) ;
  • Jun, Chang-Yeong (Koje Marine Hatchery, South Sea Fisheries Research Institute, NFRDI) ;
  • Lee, Hae-Young (Aquaculture Division, National Fisheries Research & Development Institute)
  • 정승희 (국립수산과학원 병리연구과) ;
  • 이주석 (국립수산과학원 병리연구과) ;
  • 한형균 (국립수산과학원 거제수산종묘시험장) ;
  • 전창영 (국립수산과학원 거제수산종묘시험장) ;
  • 이해영 (국립수산과학원 양식생물과)
  • Published : 2002.04.01
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Abstract

Effects of medicinal herb extract on nonspecific immune responses, hematology and disease resistance against Edwardsiella tarda in olive flounder, Paralichthys olivaceus were evaluated. Wormwood, Artemisia asiatica NAKAI and barrenwort, Epimedium koreanum NAKAI were mixed at a ratio of 7 : 3 (w/w) for 2-herbs extract and wormwood, barrenwort, Korean forsythia, Forsythia koreana NAKAI, chrysanthemum, Chrysanthemum zawadskii var. latilobum KITAMURA, peppermint, Mentha arvensis L. var, piperascens MALINV., great burnet, Snaguisorba afficinalis L., Lizard tail. Saururus chinensis BAILL., mulberry, Morus alba L., and star anise, Illicium varum HOOK, f, at the same weight for 9-herbs extract. Two-herbs of 9-herbs extract were prepared by heating after adding 10㎖ of distilled water per g of the herb mixtures. Fish (10.3$\pm$2.5g) were fed the experimental diets supplemented with the 2-herbs or 9-herbs extract at the different concentrations of 0%, 0.1%, 0.5% and 1% per kg diet for 12 weeks. Lysozyme and bactericidal activities of serum, and hematological characteristics were examined during experimental period. After feeding test period, all experimental groups were challenged with E. tarda. Lysozyme activity from the fish fed the diet supplemented with 0.1% or 0.5% of 2-herbs extract was significantly higher than the control. But there was no difference both in bactericidal activity and hematology among each group. Sixty seven % of relative percent survival values (RPS) in the group fed the diet supplemented with 0.1% of 2-herbs was higher than the other group and the control. These results suggest that supplenmentation of 0.1% of 2-herbs extract to a commercial diet may enhance disease resistance in olive flounder. Although both 0.1% and 0.5% 9-herbs extract did not improve non-specific immune reponses, they could enhance disease resistance of 53% RPS, respectively.

Keywords

References

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