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http://dx.doi.org/10.13000/JFMSE.2015.27.5.1508

Genetic Variations of Outer Membrane Protein Genes of Vibrio harveyi Isolated in Korea and Immunogenicity of OmpW in Olive Flounder, Paralichthys olivaceus  

KIM, Myoung-Sug (National Fisheries Research and Development Institute)
JIN, Ji-Woong (Korea Fisheries Resources Agency)
JUNG, Sung-Hee (National Fisheries Research and Development Institute)
SEO, Jung-Soo (National Fisheries Research and Development Institute)
HONG, Suhee (Gangneung Wonju National University)
Publication Information
Journal of Fisheries and Marine Sciences Education / v.27, no.5, 2015 , pp. 1508-1521 More about this Journal
Abstract
Vibrio harveyi is a pathogenic marine bacterium causing systemic symptoms resulting in mass mortalities in fishes and shrimps in aquaculture. Outer membrane proteins(OMPs) are related to the pathogenicity and thus good targets for diagnosis and vaccination for Gram negative bacteria. Recently vaccination strategies using the OMPs have been suggested to control vibriosis in several fish species. In this study, we have isolated V. harveyi from diseased marine fishes from different regions of Korea and investigated genetic variations of four OMP genes including OmpK, OmpU, OmpV and OmpW. Consequently, OmpK and U genes could be divided into 3 subgroups of type I, II, III and type A, B, C, respectively, without any correlation with geographical regions and species while OmpV and W were highly homologous. OmpW gene of V. harveyi FP4138 was fully sequenced and predicted the deduced amino acid sequence to form ${\beta}-barrel$ with hydrophobic channel. Indeed, the immunogenicity of recombinant OmpW produced in Escherichia coli was assessed by vaccinating flounder. As a result, the high antibody response with antibody titer of $4.2{\pm}0.7$ and protection with relative percent survival of 60% against artificial infection of V. harveyi were demonstrated. This result indicates that OmpW is a virulence related factor and it can be a vaccine candidate to prevent a high mortality caused by V. harveyi infection in olive flounder, Paralichthys olivaceus.
Keywords
Paralichythys olivaceus; Vibrio harveyi; Outer membrane protein; Genetic variation; Immungenicity;
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