• Asian Pacific Journal of Cancer Prevention
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• 제13권12호
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• pp.6391-6395
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• 2012

Background: Ovarian cancer is ranked as the fifth most common cause of cancer death in women. In Malaysia, it is the fourth most common cancer in females. CA125 has been the tumor marker of choice in ovarian cancer but its diagnostic specificity in early stages is only 50%. Hence, there is a critical need to identify an alternative tumor marker that is capable of detecting detect ovarian cancer at an early stage. HE4 is a new tumor marker proposed for the early diagnosis of ovarian cancer and disease recurrence. Currently, none of the normal ranges of HE4 quoted in the literature are based on data for a multiethnic Asian population. Therefore, the aim of this study was to determine reference intervals for HE4 in an Asian population presenting in University Malaya Medical Centre, a tertiary reference hospital. Materials and Methods: 300 healthy women were recruited comprising 150 premenopausal and 150 postmenopausal women, aged from 20-76 years. All women were subjected to a pelvic ultrasonograph and were confirmed to be free from ovarian pathology on recruitment. Serum HE4 levels were determined by chemiluminescent microparticle immunoassay (CMIA, Abbott Architect). The reference intervals were determined following CLSI guidelines (C28-A2) using a non-parametric method. Results: The upper limits of the $95^{th}$ percentile reference interval (90%CI) for all the women collectively were 64.6 pmol/L, and 58.4 pmol/L for premenopausal) and 69.0 pmol/L for postmenopausal. The concentration of HE4 was noted to increase with age especially in women who were more than 50 years old. We also noted that our proposed reference limit was lower compared to the level given by manufacturer Abbott Architect HE4 kit insert (58.4 vs 70 pmol/L for premenopausal group and 69.0 vs 140 pmol/L in the postmenopausal group). The study also showed a significant difference in HE4 concentrations between ethnic groups (Malays and Indians). The levels of HE4 in Indians appeared higher than in Malays (p<0.05), while no significant differences were noted between the Malays and Chinese ethnic groups. Conclusions: More data are needed to establish a reference interval that will better represent the multiethnic Malaysian population. Probably a larger sampling size of equal representation of the Malay, Chinese, Indians as well as the other native ethnic communities will give us a greater confidence on whether genetics plays a role in reference interval determination.

• 한국임상수의학회지
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• 제30권5호
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• pp.363-365
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• 2013

15살, 중성화 암컷 2.80 kg의 말티즈 개가 불명의 경련 및 허탈로 내원하였다. 내원시, 혈청화학적검사에서 심한 저혈당이 나타났으나 전해질검사, 전혈구검사, 뇨분석 및 방사선과 복부초음파에서는 특이적인 소견이 관찰되지 않았다. 인슐린 수치는 정상이었으며, 프락토스아민은 정상범위보다 약간 낮았다. 저혈당의 교정을 위해 프레드니솔론이 처방되었고, 임상증상은 개선되었다. 처음 내원한지 45주 후에, 저혈당성 경련의 재발로 다시 내원하였고, 이때 낮은 혈당치와 매우 높은 인슐린농도가 측정되었다. 복부 초음파 상에서, 췌장은 실질이 전반적으로 증가된 균질하지 않은 에코를 보였으며, 실질 내에 저에코성의 병변이 관찰되었다. 이러한 소견을 근거로, 이 개는 인슐린종으로 임상적 진단되었다. 더 높은 용량의 프레드니솔론을 하루에 2회씩 투여하였고, 경련 등의 임상증상은 개선되었다. 처음 내원한지 688일 후, 환자는 인슐린종으로 인한 저혈당성 경련의 재발 없이 아직 생존해있다. 이 사례는 말티즈 개에서 인슐린종의 진단과 프레드니솔론 단독으로 인슐린종의 장기간 관리에 대한 좋은 예를 보여준다.

• 한국임상수의학회지
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• 제32권6호
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• pp.481-485
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• 2015

후지 전체의 정렬 평가에 필요한 정렬각도의 정상치를 측정하기 위하여, 정형외과적 질환이 없고 육안 또는 방사선 검사에서 각기형이 없는 것으로 판단되는 소형견 사체(말티즈, 푸들, 시추, 요크셔테리어) 의 후지 60개를 사용하였다. 각각의 후지를 완전히 신장시켜 전면 방사선 사진을 촬영하였으며, 이를 통해 기계적경골대퇴골각도(mechanical tibiofemoral angle, mTFA), 기계적중족경골각도 (mechanical metatarsotibial angle (mMTTA) 기계축-대퇴각도(mechanical axis-femur angle (MAFA), 기계축-중족각도(mechanical axis-metatarsus angle (MAMTA)을 측정하였으며, 무릎관절과 뒷발목관절의 기계축변위(mechanical axis deviation, SMAD or TMAD)의 평균값과 95% 신뢰구간 값을 전체 그리고 품종별로 측정하였다. 소형견 전체의 mTFA, MTTA, MAFA, MAMTA의 95% 신뢰구간 값은 각각 $5.7-7.4^{\circ}$, $-2.2--0.8^{\circ}$, $3.5-4.5^{\circ}$, $1.0-2.0^{\circ}$였으며, SMAD와 TMAD는 2.1-2.7%, 0.5-1.0%로 측정되었다. 본 연구에서 제시한 소형견의 후지정렬 측정법과 정상치는 소형견종의 후지 각기형의 진단 및 평가, 수술 계획에 있어 유용하게 사용될 수 있을 것이라 사료된다.

• BMB Reports
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• 제40권2호
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• pp.226-231
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• 2007

Housekeeping genes are widely used as internal controls in a variety of study types, including real time RT-PCR, microarrays, Northern analysis and RNase protection assays. However, even commonly used housekeeping genes may vary in stability depending on the cell type or disease being studied. Thus, it is necessary to identify additional housekeeping-type genes that show sample-independent stability. Here, we used statistical analysis to examine a large human microarray database, seeking genes that were stably expressed in various tissues, disease states and cell lines. We further selected genes that were expressed at different levels, because reference and target genes should be present in similar copy numbers to achieve reliable quantitative results. Real time RT-PCR amplification of three newly identified reference genes, CGI-119, CTBP1 and GOLGAl, alongside three well-known housekeeping genes, B2M, GAPD, and TUBB, confirmed that the newly identified genes were more stably expressed in individual samples with similar ranges. These results collectively suggest that statistical analysis of microarray data can be used to identify new candidate housekeeping genes showing consistent expression across tissues and diseases. Our analysis identified three novel candidate housekeeping genes (CGI-119, GOLGA1, and CTBP1) that could prove useful for normalization across a variety of RNA-based techniques.

• 한국도로학회논문집
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• 제18권3호
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• pp.21-32
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• 2016

PURPOSES : In this paper, the analytical solutions suggested to simulate the behavior of rheological fluids were rigorously re-derived and investigated for fixed conditions to evaluate the applicability for the solutions on a mini-cone slump test of cement paste. The selected solutions with proper boundary conditions can be used as reference solutions to evaluate the performance of numerical simulation approaches, such as the discrete element method. METHODS : The slump, height, and spread radius for the given boundary and yield stress conditions that are determined by five different analytical solutions are compared. RESULTS : The analytical solution based on fluid mechanics for pure shear flow shows similar results to that for intermediate flow at low yield stresses. The fluid mechanics-based analytical solution resulted in a very similar trend to the geometry-based analytical solution. However, it showed a higher slump at high yield stress and lower slump at low yield stress ranges than the geometry-based analytical model. The analytical solution based on the mini-cone geometry was not significantly affected by the yield criteria, such as von Mises and Tresca. CONCLUSIONS : Even though differences among the analytical solutions in terms of slump and spread radius existed, the difference can be considered insignificant when the solutions were used as reference to evaluate the appropriateness of numerical approaches, such as the discrete element method.

• 한국소음진동공학회논문집
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• 제15권3호
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• pp.297-305
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• 2005

A high-speed spindle can be very sensitive to rotating mass unbalance which has harmful effect on many machine tools. Therefore, the balancing procedure to reducevibration in rotating system is certainly needed for all high-speed spindles. An active balancing program using influence coefficient method and an active balancing device of an electro-magnetic type have been applied to the developed high-speed spindle system in this study. A reliable gain-scheduling control using influence coefficients of the reference model although system characteristics are changed is applied. The stability of reference influence coefficients is verified by frequency response functions. The active balancing experiment for the developed high-speed spindle during operation is well performed with an active balancing program and device. As a result, controlled unbalance responses are below the vibration limit at all rotating speed ranges with critical speed.

• Geomechanics and Engineering
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• 제19권5호
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• pp.407-420
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• 2019

This paper illustrates the results of a series of seismic geotechnical centrifuge experiments to explore dynamic structure-soil-structure interaction (SSSI) of two structures (named S1 and S2) installed on ground surface. A dense homogeneous ground is prepared in an equivalent shear beam (ESB) container. Two structural models are designed to elicit soil-foundation-structure interaction (SFSI) with different masses, heights, and dynamic characteristics. Five experimental tests are carried out for: (1) two reference responses of the two structures and (2) the response of two structures closely located at three ranges of distance. It is found that differential settlements of both structures increase and the smaller structure (S2) inversely rotates out of the other (S1) when they interact with each other. S2 structure experiences less settlement and uplift when at a close distance to the S1 structure. Furthermore, the S1 structure, which is larger one, shows a larger rocking and a smaller sliding response due to the SSSI effects, while S2 structure tends to slide more than that in the reference test, which is illustrated by an increase in sliding response and rocking stiffness as well as a decrease in moment-to-shear ratio (M/H·L) of the S2 structure.

• 한국추진공학회:학술대회논문집
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• 한국추진공학회 2008년도 제31회 추계학술대회논문집
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• pp.117-121
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• 2008

슈라우드가 장착된 초음속 충동형 터빈의 팁간극에 따른 성능변화를 시험적으로 고찰하였다. 시험은 30톤급 터보펌프용 터빈을 이용하였으며 고압공기를 이용하여 다양한 압력비 및 회전수 범위에서 이루어졌다. 터빈의 효율민감도는 기준시험점에서 0.05의 매우 작은 값을 나타내었다. 터빈효율변화는 간극의 크기에 다라 비선형적으로 변화하였으며 고정된 압력비에서 효율민감도는 회전수의 세제곱에 비례하는 것으로 나타났다. 아울러 회전수에 무관하게 효율민감도는 기준시험 압력비에서 최소값을 보이는 것으로 나타났다.

• 한국임상수의학회지
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• 제28권4호
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• pp.365-368
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• 2011

Next to performing an expert physical examination, a blood sample submitted for a complete blood count is the most basic tool available to owners or veterinary practitioners. Reference values of complete blood count were determined at 6 different ages in 114 Thoroughbred foals during the second month of life. Hematologic results were as follows: RBC 8.2-10.5 ($10^6/{\mu}l$), Hb 10.9-13.3 (g/dl), HCT 28.2-35.2 (%), MCV 30.7-35.8 (fL), MCH 11.9-13.5 (pg), MCHC 37.9-40.5 (g/dl), RDW 24.5-25.7 (%), PLT 146.3-256.4 ($10^3/{\mu}l$), MPV 6.7-8.3(fL), total WBC 8.1-12.5 ($10^3/{\mu}l$), basophils 0.0 ($10^3/{\mu}l$), eosinophils 0.0-0.3 ($10^3/{\mu}l$), neutrophils 2.4-8.6 ($10^3/{\mu}l$), lymphocytes 1.8-2.9 ($10^3/{\mu}l$) and monocytes 0.0-1.4 ($10^3/{\mu}l$). The results of this study serve as reference ranges for Thoroughbred neonatal foals populations and can be useful for health control, regular examination and pre-sale soundness examination.

• Nutrition Research and Practice
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• 제5권3호
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• pp.266-274
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• 2011

Accurate assessment of nutrient adequacy of a population should be based on usual intake distribution of that population. This study was conducted to adjust usual nutrient intake distributions of a single 24-hour recall in 2001 Korean National Health and Nutrition Surveys (KNHNS) in order to determine the magnitude of limitations inherent to a single 24-hour recall in assessing nutrient intakes of a population. Of 9,960 individuals who provided one 24-hour recall in 2001 KNHNS, 3,976 subjects provided an additional one-day 24-hour recall in 2002 Korean National Nutrition Survey by Season (KNNSS). To adjust for usual intake distribution, we estimated within-individual variations derived from 2001 KNHNS and 2002 KNNSS using the Iowa State University method. Nutritionally at risk population was assessed in reference to the Dietary Reference Intakes for Koreans (KDRIs). The Korean Estimated Average Requirement (Korean EAR) cut-point was applied to estimate the prevalence of inadequate nutrient intakes except for iron intakes, which were assessed using the probability approach. The estimated proportions below Korean EAR for calcium, riboflavin, and iron were 73%, 41%, and 24% from usual intake distribution and 70%, 51%, and 39% from one-day intake distribution, respectively. The estimated proportion of sodium intakes over the Intake Goal of 2,000 mg/day was 100% of the population after adjustment. The energy proportion from protein was within Korean Acceptable Macronutrient Distribution Ranges (Korean AMDR), whereas that of carbohydrate was higher than the upper limit and that of fat was below the lower limit in the subjects aged 30 years or older. According to these results, the prevalence of nutritional inadequacy and excess intake is over-estimated in Korea unless usual intake distributions are adjusted for one-day intakes of most nutrients.