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Physiological and Ecological Characteristics of Hemolytic Vibrios and Development of Sanitary Countermeasure of Raw Fisheries Foods 3. Growth Factor and Antibiotic Susceptibility of Vibrio cholerae non-O1 FM-3 Isolated from Sea Water (용혈독소를 생산하는 기수성 비브리오균의 생리${\cdot}$생태적 특성과 수산식품의 위생대책 3. 해수에서 분리된 Vibrio cholerae non-O1 FM-3의 생육인자와 항생제 감수성)

  • KIM Shin-Hee;PARK Mi-Yeon;PARK Uk-Yeon;KIM Young-Man;CHANG Dong-Suck
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.30 no.4
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    • pp.550-555
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    • 1997
  • Vibrio cholerae non-O1 (V. cholerae non-O1) was previously called nonagglutinable or noncholera vibrios, since it fails to react with polyvalent O1 antisera. This organism is biochemically and genetically indistinguishable from V. cholerae O1 except serological difference. V. cholerae non-O1 strains are often detected in the environment including bays, estuaries, and fresh water, and also found in food. Therefore it is designated food borne bacterium in Japan. However, research papers on V. cholerae non-O1 are very rare in Korea. In order to investigate bacteriological characteristics of V. cholerae non-O1, we isolated V. cholerae non-O1 from the environmental sea water. Among the isolated V. cholerae non-O1 strains, we selected the strain which had the most strong hemolytic activity, named as V. cholerae non-O1 FM-3. The optimum growth conditions of V. cholerae non-O1 FM-3 were $37^{\circ}C$ and pH 8.5 in BHI broth (containing $0.5\%$ sodium chloride), and it grew better than V. cholerae non-O1 ATCC 25872. But both were not able to grow in BHI broth added $5.0\%$ of sodium chloride or adjusted to pH 5.0. According to the experimental results on the susceptibility test against various antibiotics, there were no significant differences between the isolated strain and reference strain (V. cholerae non-O1 ATCC 25872). Most of the antibiotics examined had bacteriostatic action against V. cholerae non-O1 FM-3 while vancomycin, oxacillin, colistin, polymyxin B, and sulfadiazine had no bacteriostatic activity.

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A Study of the Analysis of Citizen's Awareness of the Transforming of a Former Military Site into Urban Park - With Special Reference to the City of Euijungbu - (도시 군부대 이전 적지의 공원화 방향에 대한 시민인식 분석 - 의정부시 사례연구 -)

  • Maeng, Chi-Young;Cho, Se-Hwan
    • Journal of the Korean Institute of Landscape Architecture
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    • v.37 no.2
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    • pp.62-69
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    • 2009
  • This study aimed to analyze citizen awareness of the transforming of a former military site in the city into use as an urban park, and to determine the direction of park creation in the context of urban regeneration. The study focused on the city of Uijungbu, so-called "representative military city" of Korea, which has over eight US military army corps in CBD and which islocated on the northside of Seoul. The questionnaire survey was carried out in the year of 2006, during two months from 1 June till 30 July. The research was carried out by book review as a theoretical base and a questionnaire survey to analyze citizen awareness. The conclusions of this study were as follows. 1. Areas that have been transformed from a former military site to large urban parks for the promotion of economic, social, environmental, and aesthetical value in the context of urban regeneration include Downsview Park(Toronto, Canada), and Great Park(Irvine, CA U.S.A). 2. The citizens of Euijungbu emphasized having casual rest and recreational functions in an urban park, but were also concerned with the promotion of cultural image, activation of the city's economy, etc. 3. The citizens preferred to use the former military site for a park instead of for residential and commercial purposes to introduce cultural facilities and encourage economic activation. 4. All results of this study proposed to use the former military site for encouraging and activating the urban economy, cultural promotion, social reconciliation and aesthetic value by the transforming the site into a large, multi-use park in the context of urban regeneration.

Identification of the Environmentally Problematic Input/Environmental Emissions and Selection of the Optimum End-of-pipe Treatment Technologies of the Cement Manufacturing Process (시멘트 제조공정의 환경적 취약 투입물/환경오염물 파악 및 최적종말처리 공정 선정)

  • Lee, Joo-Young;Kim, Yoon-Ha;Lee, Kun-Mo
    • Journal of Korean Society of Environmental Engineers
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    • v.39 no.8
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    • pp.449-455
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    • 2017
  • Process input data including material and energy, process output data including product, co-product and its environmental emissions of the reference and target processes were collected and analyzed to evaluate the process performance. Environmentally problematic input/environmental emissions of the manufacturing processes were identified using these data. Significant process inputs contributing to each of the environmental emissions were identified using multiple regression analysis between the process inputs and environmental emissions. Optimum combination of the end-of-pipe technologies for treating the environmental emissions considering economic aspects was made using the linear programming technique. The cement manufacturing processes in Korea and the EU producing same type of cement were chosen for the case study. Environmentally problematic input/environmental emissions of the domestic cement manufacturing processes include coal, dust, and $SO_x$. Multiple regression analysis among the process inputs and environmental emissions revealed that $CO_2$ emission was influenced most by coal, followed by the input raw materials and gypsum. $SO_x$ emission was influenced by coal, and dust emission by gypsum followed by raw material. Optimization of the end-of-pipe technologies treating dust showed that a combination of 100% of the electro precipitator and 2.4% of the fiber filter gives the lowest cost. The $SO_x$ case showed that a combination of 100% of the dry addition process and 25.88% of the wet scrubber gives the lowest cost. Salient feature of this research is that it proposed a method for identifying environmentally problematic input/environmental emissions of the manufacturing processes, in particular, cement manufacturing process. Another feature is that it showed a method for selecting the optimum combination of the end-of-pipe treatment technologies.

A Study on the Comparative Art theory in the Arts and Crafts Movement and Post-impressionism (미술 공예 운동과 후기 인상주의 비교 예술론 연구)

  • 박연실
    • Archives of design research
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    • v.20
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    • pp.279-291
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    • 1997
  • The history of modern design begins with the arts and crafts movement(1860). The importance of the movement which decorated the outset gave birth to all the trends of thought which would occur under the circumstances within it, and is deemed that the ideas of the figures who played an active part in the movement might be ceaselessly continued through the works and ideas of their outstanding juniors or purpils as a doctrine of the philosophy of design. Therefore, it might be a prerequisite that the significance and spirit of the movement, and its developing process should be addressed in detail in the first place, but for the limited space of this paper, it was intended to desvribe only the part which can be interpreted in duplicate, linked with post-impressionism. The subject of this thesis is about a theory of art in which both ideas of the movement(1860) and the post-impressionism(1910) are comparatively reviewed. The genre, strictly speaking, is classified into the history of painting, and there is a gap fo about an half century between the issues which are comparatively discussed here. Both the movements began in a same environmental place of England, specially the movement at Milieu in England, and since there is a common point that the representative runners of each movement, William Morries(1834-1896) and Roger Fry(1866-1934), belong to a same race of Engol-Saxon, their ideas coincide with an aesthetic scholar, H. Tanie's aesthetic interpretation method and the more important is, as being elucidated in the comment and aesthetic theory for which Roger Fry gave effort and activity in his later life, that when he read intensively Ruskin's books, $\ulcorner$Modern Painters$\lrcorner$and$\ulcorner$Stones of Venice$\lrcorner$he had solidified his idea of post-impressionism while giving approval and criticism on them. After all, as in a co-painter, Windyham Lewis's reference of 'Roger Fry's Too Late Morris Movement', he, inspired by the actual activities of Morris, played activities similar to that of Morris in which exhibiting and selling some of his decorative art works signed by him and the works of post-impressionism through (1913-1920). Herein, that is wished to add a remark by this author is a point that the author of$\ulcorner$Vision and Design$\lrcorner$, Roger Fry, has not be made a subject of discussion specially in the Korean world of design. So, with this case of a thesis, it's wished that many latent awakened, design persons in korea give efforts to researching into Roger Fry so that their findings could be officially announced in the would. By the way, what is tried to describe in this paper from now on is to analyse and review the origin of post-impressionism which idea was first coined in the art world through the 1st and 2nd exhibitions of 'Manet and Post-impressionism' which were opened each at the Grafton Gallery in 1910 and 1913 by him. And also, it is intended to review it through the art journals and some references by critics of the day in which favorable criticism or severe criticism were ready to comment through the opinions and influences of the coworkers of Roger Fry, say, Clive Bell, Desmond Maccarthy, etc. and of himself as a main axis, on the art ideas of Gauguin, Gogh, Matisse, and Cezanne whose works were the typical ones participated and exhibited in those 1st and 2nd exhibitions.

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A Study on 'Verfremdung' effects in visual advertisements with a special reference to the <017-I-Touch series, The Hand Transformed into a Web >, mobile network advertisement (동영상 광고에서 나타나는 '소격화' 효과에 관한 연구 - 광고 <017-I-Touch편 (손이 물갈퀴) >를 중심으로 -관어영시광고중소출현적‘맥생화’효과적연구(關於影視廣告中所出現的‘陌生化’效果的硏究))

  • Jin, Ri-Long;Ahn, Sang-Soo;Kim, Jong-Deok
    • Archives of design research
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    • v.18 no.2 s.60
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    • pp.37-46
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    • 2005
  • Today's advertisement is dominated by visual images, which deliver messages in the most efficient and engaging way. Vivid and live images, through multimedia, attract the viewer to consume the products being advertised. In this course, the effect of 'Verfremdung' or alienation is often detected. This terminology was at first used in a epic play of Bertolt Brecht to signify a specific effect to block the empathy of the audience by reminding them of their position as the audience separate from the play. 'Verfremdung' helps them to have a sense of objectivity and critical attitude toward the performance. Multimedia commercials nowadays exhibit such a quality together with 'viewer participation:' criticism on reality: and 'speed.' In an advertisement for Shinsegi Communications' mobile system titled <017-I-Touch series, the hand transformed into a web>, the copy, 'my blood type is 'i', ' is accompanied by six unrelated fictitious scenes in which the same ocean appears as a common denominator. Because there is no connection between the scenes, free imagination of the viewer has to be involved and thus plays a significant role in making them into a context. This fact dearly exhibit some characteristics of post- modern advertisement. Momentary 'Gap' and 'Difference' between scenes contribute to 'Verfremdung' or alienation' that makes it hard for the consumers to comprehend the content on the spot. Such an uneasy situation, however, keeps the viewers thinking about the advertisement itself. While repeatedly exposed to the alienated images, the viewers come to get involved in the advertisement, trying to make the fragmented images into a coherent context. In addition, the leaps between the scenes produce a sense of 'speed' in a context, which adds more impact to the way of delivering messages using multimedia. With the help of multimedia, 'Verfremdung: which was originally intended to bring about objective and critical altitude of the audience in a play, plays a crucial role in attracting the viewer's attention and conveying a specific message in a moment in contemporary advertisement.

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Analysis of Expression Types of Character Animation TV Advertisements (캐릭터애니메이션 TV광고의 표현유형 분석 연구)

  • Lee, Yong-Woo
    • Archives of design research
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    • v.19 no.5 s.67
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    • pp.85-94
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    • 2006
  • In the 2000s, character animation technique is used at domestic TV character animation ads of diverse items displaying new character patterns and expression techniques of a high level. This study intends to identify the characteristics of TV character animation ads based ell character aesthetic theory and advertising rhetoric theory, and based on these findings, analyze trend and stream of expression in TV character animation ads by examining and classifying advertisements released in the past four years by items, character patterns and expression techniques. The results showed that TV character animation ads have been concentrated on confectionery, beverages and foods in the past, but the items have been diversified through the years. With reference to character patterns, personal and animal characters had made the main pattern, but recently product, virtual and composite characters are on the increase. In expression techniques, cell animation technique was found to be the most frequently used technique, which was followed by full-3D, cell and live action, cell and 3D and the clay animation technique. A trend like this suggests that TV character animation advertising items will continue to increase in line with the participation level of targets. In the meantime, personal and animal characters will continue to make the leading character pattern, while virtual and composite character patterns emerge in new forms. In expression techniques, live action combined with 30 technique and techniques using new expression materials will appear with cell animation technique as a central figure. As a whole, Korean character animation advertisement is expected to keep on growing to a new dimension in the aspect of items, character patterns and expression technique.

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Volatilization of Sprayed Pesticides in Greenhouse using a Lysimeter (라이시미터를 이용한 시설하우스 내에 살포한 농약의 휘산 양상)

  • Kim, Danbi;Kim, Taek-Kyum;Kwon, HyeYong;Hong, Su-Myeong;Park, Byung-Jun;Lim, Sung-Jin;Lee, Hyo-Sub;Moon, Byeong-Cheol
    • The Korean Journal of Pesticide Science
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    • v.20 no.4
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    • pp.305-311
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    • 2016
  • In cultivation environment, various pesticides are used and some of them could be volatilized into the air. This could affect farmer's health and also cause environmental pollution. This study was carried out to investigate the volatilization of pesticides, and use the reference data for preventing farmer's pesticide intoxication and securing worker safety. The experiment was conducted in a greenhouse using a lysimeter which was of $1m^2$ area and 1.5 m depth filled with upland soil. The pesticides treated in lysimeter soil were ethoprophos (5.0% GR), diazinon (34.0% EC), alachlor (43.7% EC), metolachlor (40.0% EC), chlorpyrifos (2.0% GR), pendimethalin (31.7% EC), carbaryl (50.0% WP), napropamide (50% WP), tebuconazole (25.0% WP) and imidacloprid (2.0% GR). Each pesticide was treated at a concentration of 770.5 mg based on A.I (%). The recovery of pesticide ranged from 77.4 to 99.3%. The volatilized pesticides in air were collected by personal air sampler with PUF tube at 4 l/min flow rate. In addition, temperature and humidity were measured. The collected samples were extracted using acetone in a soxhlet apparatus for 8 hours. The extracted pesticides were resoluted with acetonitrile and diluted 5 times. It was analyzed with LC-MS/MS. For 720 hours experiment, the largest vaporization amount of each pesticide in air was ethoprophos $15.24{\mu}g/m^3$, diazinon $5.14{\mu}g/m^3$, pendimethalin $2.70{\mu}g/m^3$, chlorpyrifos $1.76{\mu}g/m^3$, alachlor $1.40{\mu}g/m^3$, metolachlor $1.12{\mu}g/m^3$, carbaryl $0.27{\mu}g/m^3$, napropamide $0.22{\mu}g/m^3$, tebuconazole $0.11{\mu}g/m^3$ and imidacloprid $0.05{\mu}g/m^3$. The R value (coefficient of correlation) between volatilization and vapor pressure of pesticides is higher than 0.99. Therefore, there is high correlation between volatilization and vapor pressure of pesticides.

The feasibility evaluation of Respiratory Gated radiation therapy simulation according to the Respiratory Training with lung cancer (폐암 환자의 호흡훈련에 의한 호흡동조 방사선치료계획의 유용성 평가)

  • Hong, mi ran;Kim, cheol jong;Park, soo yeon;Choi, jae won;Pyo, hong ryeol
    • The Journal of Korean Society for Radiation Therapy
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    • v.28 no.2
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    • pp.149-159
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    • 2016
  • Purpose : To evaluate the usefulness of the breathing exercise,we analyzed the change in the RPM signal and the diaphragm imagebefore 4D respiratory gated radiation therapy planning of lung cancer patients. Materials and Methods : The breathing training was enforced on 11 patients getting the 4D respiratory gated radiation therapy from April, 2016 until August. At the same time, RPM signal and diaphragm image was obtained respiration training total three steps in step 1 signal acquisition of free-breathing state, 2 steps respiratory signal acquisition through the guide of the respiratory signal, 3 steps, won the regular respiration signal to the description and repeat training. And then, acquired the minimum value, maximum value, average value, and a standard deviation of the inspiration and expiration in RPM signal and diaphragm image in each steps. Were normalized by the value of the step 1, to convert the 2,3 steps to the other distribution ratio (%), by evaluating the change in the interior of the respiratory motion of the patient, it was evaluated breathing exercise usefulness of each patient. Results : The mean value and the standard deviation of each step were obtained with the procedure 1 of the RPM signal and the diaphragm amplitude as a 100% reference. In the RPM signal, the amplitudes and standard deviations of four patients (36.4%, eleven) decreased by 18.1%, 27.6% on average in 3 steps, and 2 patients (18.2%, 11 people) had standard deviation, It decreased by an average of 36.5%. Meanwhile, the other four patients (36.4%, eleven) decreased by an average of only amplitude 13.1%. In Step 3, the amplitude of the diaphragm image decreased by 30% on average of 9 patients (81.8%, 11 people), and the average of 2 patients (18.2%, 11 people) increased by 7.3%. However, the amplitudes of RPM signals and diaphragm image in 3steps were reduced by 52.6% and 42.1% on average from all patients, respectively, compared to the 2 steps. Relationship between RPM signal and diaphragm image amplitude difference was consistent with patterns of movement 1, 2 and 3steps, respectively, except for No. 2 No. 10 patients. Conclusion : It is possible to induce an optimized respiratory cycle when respiratory training is done. By conducting respiratory training before treatment, it was possible to expect the effect of predicting the movement of the lung which could control the patient's respiration. Ultimately, it can be said that breathing exercises are useful because it is possible to minimize the systematic error of radiotherapy, expect more accurate treatment. In this study, it is limited to research analyzed based on data on respiratory training before treatment, and it will be necessary to verify with the actual CT plan and the data acquired during treatment in the future.

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A Study on the Relationship of Learning, Innovation Capability and Innovation Outcome (학습, 혁신역량과 혁신성과 간의 관계에 관한 연구)

  • Kim, Kui-Won
    • Journal of Korea Technology Innovation Society
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    • v.17 no.2
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    • pp.380-420
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    • 2014
  • We increasingly see the importance of employees acquiring enough expert capability or innovation capability to prepare for ever growing uncertainties in their operation domains. However, despite the above circumstances, there have not been an enough number of researches on how operational input components for employees' innovation outcome, innovation activities such as acquisition, exercise and promotion effort of employee's innovation capability, and their resulting innovation outcome interact with each other. This trend is believed to have been resulted because most of the current researches on innovation focus on the units of country, industry and corporate entity levels but not on an individual corporation's innovation input components, innovation outcome and innovation activities themselves. Therefore, this study intends to avoid the currently prevalent study frames and views on innovation and focus more on the strategic policies required for the enhancement of an organization's innovation capabilities by quantitatively analyzing employees' innovation outcomes and their most suggested relevant innovation activities. The research model that this study deploys offers both linear and structural model on the trio of learning, innovation capability and innovation outcome, and then suggests the 4 relevant hypotheses which are quantitatively tested and analyzed as follows: Hypothesis 1] The different levels of innovation capability produce different innovation outcomes (accepted, p-value = 0.000<0.05). Hypothesis 2] The different amounts of learning time produce different innovation capabilities (rejected, p-value = 0.199, 0.220>0.05). Hypothesis 3] The different amounts of learning time produce different innovation outcomes. (accepted, p-value = 0.000<0.05). Hypothesis 4] the innovation capability acts as a significant parameter in the relationship of the amount of learning time and innovation outcome (structural modeling test). This structural model after the t-tests on Hypotheses 1 through 4 proves that irregular on-the-job training and e-learning directly affects the learning time factor while job experience level, employment period and capability level measurement also directly impacts on the innovation capability factor. Also this hypothesis gets further supported by the fact that the patent time absolutely and directly affects the innovation capability factor rather than the learning time factor. Through the 4 hypotheses, this study proposes as measures to maximize an organization's innovation outcome. firstly, frequent irregular on-the-job training that is based on an e-learning system, secondly, efficient innovation management of employment period, job skill levels, etc through active sponsorship and energization community of practice (CoP) as a form of irregular learning, and thirdly a model of Yί=f(e, i, s, t, w)+${\varepsilon}$ as an innovation outcome function that is soundly based on a smart system of capability level measurement. The innovation outcome function is what this study considers the most appropriate and important reference model.

Exploration of Virulence Markers and Genes of Listeria monocytogenes Isolated from Animal Products (축산물유래 Listeria monocytogenes의 virulence marker 및 gene 조사)

  • Yi, Chul-Hyeon;Song, Hyeon-Ho;Kim, Mi-Ryung;Kang, Ho-Jo;Son, Won-Geun
    • Journal of Food Hygiene and Safety
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    • v.23 no.3
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    • pp.248-256
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    • 2008
  • To investigate the epidemiological characteristics of 68 Listeria monocytogenes isolates, including 11 reference strains and 57 isolates from imported US beef, domestic meats(beef, pork, chicken meat), raw milk, and milk plants. L. monocytogenes was to evaluate the production of virulence proteins, such as hemolysin(LLO) and lecithinase(LCP), the adsorption of Congo red(CRA), and to detect virulence genes using the polymerase chain reaction(PCR). In the study of virulence protein production, 68(100%), 62(91.2%), and 54(79.4%) of the 68 L. monocytogenes strains were positive for LLO production, the LCP test, and the CRA test, respectively, while strains of other species, such as L. innocua, L. gray, L. murrayi, and L. welshimeri, were not. There were no significant differences between L. monocytogenes serotypes and the ability to produce LLO or LCP. L. monocytogenesstrains had very high hemolytic titers(2 to 16 fold), while the other Listeria species, other than L. ivanovii and L. seeligeri, did not. The hemolysin activities of L. monocytogenes, L. ivanovii, and L. seeligeri usually exceeded 1.0 HU/mg, while those of other Listeria spp. were less than 0.04 HU/mg. In the PCR assay, all of the L. monocytogenes strains contained the hlyA, plcA, plcB, inlA, and inlB virulence genes and produced a product of the expected size. In the PCR of the actA gene, the expected 385-bp product was seen in 39(57.4%) L. monocytogenesstrains, while an unexpected 268-bp product was seen in 29(42.6%) strains. Most L. monocytogenes strains isolated from Hanwoo beef produced the 385-bp actA gene product, while strains of imported US beef usually produced the 268-bp actA gene product. By contrast, no virulence gene products were amplified in the other Listeria spp.