• Child Studies in Asia-Pacific Contexts
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• v.6 no.1
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• pp.31-46
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• 2016

This study explored types and characteristics of South Korean crossover picturebooks that are enjoyed across generations. Participants included three publishers, six critics, six illustrators and three picturebook researchers, and focused on 92 South Korean recommended picturebooks published from 1995 to 2014 as the research material for this study. The majority of Korean crossover picturebook type was story, followed by information and art. There were few wordless books. Common themes of the story picturebooks were contemplation, traditional culture, social changes (such as immigration and redevelopment), reminiscence, social relations, loss and death, family problems, and social incidents. Classic essays and novels were revised for picturebooks as were famous poems originally written for grown-ups. Informational books were about traditions in music, architecture, furniture and special occasions like wedding and ceremonies. The style of the drawings were precise and realistic. Some drawings were done by brush and Chinese ink on hanji (traditional Korean paper) or silk. Some books featured Korean calligraphy as well, enabling adult readers to also appreciate the beauty and delicacy of the books. Art books and wordless books were quite rare and exhibited a playful tone. Adults alone were not presumed to be the primary reading audience of the picture books. Implications were made for picturebook marketing in a society such as South Korea, where the elderly population is rapidly increasing. Various forms of art books and parodies were also welcome. One conclusion of the study was that more experimental and innovative works would be encouraging for the development of South Korean crossover picturebooks.

• Journal of the Korean Home Economics Association
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• v.42 no.11
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• pp.17-30
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• 2004

The purpose of this study was to explore the normative expectations of positive emotions such as happiness and pride in Korean culture by analyzing the children's textbooks in the Chosen Dynasty period. The method of this study was document analysis and the materials for analysis were 'Dongmongsensp', 'Gyukmongyogyul', 'Myungimbogam', and 'Sohak', that were the textbooks for children's education and invoked the ethics of individual life based on Confucianism. The analysis was focused on the antecedent events, emotional consequences, and emotional regulation in the emotional process of happiness and pride. According to the analysis, happiness was caused by the accomplishment of cultural tasks such as supporting parents, observing laws, being moderate, improving oneself with reading good books, and modeling good deeds, as well as parents' love, which had desirable consequences. Hence, the emotional regulation was to enhance happiness, but far as immoderate experience of happiness, the regulatory rule was expected to suppress it. Also, the antecedents of pride were those demonstrating to have power or occupying a high social position, and the consequences of pride led to self-destruction, and pride was therefore not allowed to be expressed. Consequently, happiness was enhanced because it motivated individuals to accomplish cultural tasks of interdependence, whereas pride was inhibited because it tended to disengage self from social connectedness.

• Journal of the Korean BIBLIA Society for library and Information Science
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• v.24 no.2
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• pp.5-30
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• 2013

The purpose of this study is to present a management improvement suggestion for better user satisfaction. For this, this research looked into the current issues of public libraries with literature review. Then a survey of eight hundred users in four libraries in Y-gu, D Metropolitan City was conducted to figure out users' needs. As a result, the purpose of use, visit frequency and programs demanded are turned out to show statistically significant differences. Based on this result, finally, this study suggests an advisable management direction for public libraries and practical ways to improve user satisfaction.

• Proceedings of the Korean Institute of Interior Design Conference
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• 2003.05a
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• pp.170-173
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• 2003

Because effect about environmental effect that get to human visually is absolute, ability that mural of subway interior considers relation with space is required. Also, public environment element should be designed must intend agreeableness sex by same traitor effect of quality of the material and color and can be evaluated by cultural inheritance that is powerful enemy same time. Wish to accomplish access as meaning of city environment improvement of wall of subway station or underground space etc. through Japan course South Korea's illustration regarding environment landscaping features that is designed in wall of latest domestic building subway interior in this research. Various and new reading by that study and analyze manufactured wall environment landscaping features recently that could find and was approached in variouser technique, practical use of material than single material and is expressed according to history's special quality and planning know can. Underground space can give agreeableness feel and psychological sense of security by user though expression method, material, color plan etc. consist because is having image blockade enemy. Therefore, wall environment landscaping features of underground space to be cultural value and environment Dija because diversified research that is accompanied in element is gone subway interior space of our country and underground space etc. as new culture space develop be able to must.

• Journal of Photoscience
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• v.7 no.2
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• pp.53-58
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• 2000

We performed the cloning of a chalcone synthase (CHS) gene, the key enzyme in the anthocyanin biosynthesis, from the cDNA library constructed with grape suspension cells irradiated UV-B. The PCR fragment was used to cloning the CHS gene. One CHS cDNA clone containing an open reading frame and a partial stilbene synthase (STS)cDNA, the stilbene-type phytoalexin, were isolated. The CHS cDNA clone (VCHS) showed 87% sequence homology with VvCHS (V.vinifea) and 72.3% identity with VSTSY(V.vinifea). its amino acid sequences were longer than any other CHS genes as 454 residues. Two genes were weakly expressed in white light irradiated cells, but highly induced in UV-B irradiated condition during 32 hours. Interestingly, the STS was quickly and abundantly expressed from 2 hours when supplemented with jasmonic acid (JA) and the maximum expression was observed at 4 hours and then gradually decreased. But, the additional UV-B or white light quickly degraded the STS expression than only JA treated grape suspension cells. The CHS also was rapidly induced with JA and the synergistical effect was observed at the addigional light treatment of UV-B or white light. These results are indicated that CHS and STS have different response mechanisms against the environmental stresses.

• Journal of Microbiology and Biotechnology
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• v.24 no.6
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• pp.835-842
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• 2014

Haloperoxidase (HPO, E.C.1.11.1.7) is a metal-containing enzyme oxidizing halonium species, which can be used in the synthesis of halogenated organic compounds, for instance in the production of antimicrobial agents, cosmetics, etc., in the presence of halides and $H_2O_2$. To isolate and evaluate a novel non-metal HPO using a culture-independent method, a cassette PCR library was constructed from marine seawater in Japan. We first isolated a novel HPO gene from Pseudomonas putida ATCC11172 by PCR for constructing the chimeric HPO library (HPO11172). HPO11172 showed each single open-reading frame of 828 base pairs coding for 276 amino acids, respectively, and showed 87% similarity with P. putida IF-3 sequences. Approximately 600 transformants screened for chimeric genes between P. putida ATCC11173 and HPO central fragments were able to identify 113 active clones. Among them, we finally isolated 20 novel HPO genes. Sequence analyses of the obtained 20 clones showed higher homology genes with P. putida or Sinorhizobium or Streptomyces strains. Although the HPO A9 clone showed the lowest homology with HPO11172, clones in group B, including CS19, showed a relatively higher homology of 80%, with 70% identy. E. coli cells expressing these HPO chimeric genes were able to successfully bioconvert chlorodimedone with KBr or KCl as substrate.

• Journal of Microbiology and Biotechnology
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• v.8 no.1
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• pp.14-20
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• 1998

Syntheses of the B. stearothermophilus xylanolytic enzymes such as xylanases, ${\beta}$-xylosidases, ${\alpha}$-arabinofurano-sidases, and esterases, were observed to be regulated by the carbon source present in the culture media. Xylan induced synthesis of ${\beta}$-xylosidase at the highest level while xylose gave about 30% of the ${\beta}$-xylosidase activity induced by xylan. The lowest syntheses of the xylanolytic enzymes above mentioned were detected in the basal medium containing glucose as a sole carbon source. When a mixture of xylan and glucose was used as a carbon source, we could observe glucose repression of xylanase (about 70-fold) and ${\beta}$-xylosidase (about 40-fold) syntheses. Whereas, the level of the glucose repression of the expression of the xylA gene encoding the major ${\beta}$-xylosidase of B. stearothermophilus was assessed to be about l0-fold when the relative amounts of the xylA transcript were determined. From the sequence of the xylA gene, we could find two CRE-like sequences (CRE-l: nucleotides ＋124 to ＋136 and CRE-2:＋247 to ＋259) within the reading frame of the xylA gene, either or both of which were suspected to be involved in catabolite repression of the xylA gene.

• Journal of Microbiology and Biotechnology
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• v.12 no.1
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• pp.25-30
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• 2002

A lipase of Staphylococcus aureus B56 was purified from a culture supernatant and its molecular weight was estimated to be 45 kDa by SDS-PAGE. The optimum temperature and pH for the hydrolysis of olive oil was $42^{\circ}C$ and pH 8-8.5, respectively. The enzyme was stable up to $55^{\circ}C$ in the presence of $Ca^++$ at pHs 5-11. The lipase gene was cloned using the PCR amplification method. The sequence analysis showed an open reading frame of 2,076 bp, which encoded a preproenzyme of 691 amino acids. The preproenzyme was composed of a signal sequence (37 aa), propeptide (255 aa), and mature enzyme (399 aa). Based on a sequence comparison, lipase B56 constituted of a separate subgroup among the staphylococcal lipase groups, such as S. aureus PS54 and S. haemolyticus L62 lipases, and was distinct from other lipases in their optimum pH and substrate specificity.

• Journal of Microbiology and Biotechnology
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• v.10 no.2
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• pp.221-226
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• 2000

Cephalosporin-C deacetylase (CAH) catalyzes the deacetylation of cephalosporin derivatives. A novel gene encoding the CAH from Bacillus sp. KCCM10143 was cloned and sepuenced. The uncleotide sequence contained an open reading frame encoding a polypeptide consisting of 217 amino acids and a molecular weight of 24 kDa which was in good agreement with the value obtained by sodium dodecylsulfate-polyacrylamide gel electrophoresis. An expression plasmid was constructed by inserting the CAH gene into the region of the pTrc99A expression vector. An active from of the CAH protein was expressed in the soluble fraction obtained after cell disruption. in fermentation using a 5-1 jar fementer, the transformant E. coli JM109 (pDST654) produced 4.12 U of CAH per ml of culture during 16 h of incubation.

• Journal of The Korean Digital Architecture Interior Association
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• v.9 no.1
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• pp.49-59
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• 2009

This study examined the programs of public library and the spaces for them in Seoul and Gyunggi area. The examination was performed by making field surveys on 12 sample public libraries and posing user questionnaires on 4 libraries out of the sample. The data from the questionnaire survey were processed and analyzed with SPSS 14.0. The results are as follows: First, according to the arrangement of the furniture, the space usages for the programs in public libraries are divided into four classes, one-way exposure: free of furniture: two-way exposure. The one-way exposure has been the most common though the furniture arrangement varies with the characteristics of a program. Second, language or information technology related programs are the most frequently attended programs in public libraries. Lastly, the space usage for the reading education programs has had the highest level of the user satisfaction measurement while the user satisfaction is lower in those for general culture programs and health care programs.