• Title/Summary/Keyword: Re-Identification

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Availability of Identification by RAPD of Aspergillus species from Sputum (객담에서 분리한 Aspergillus 속의 RAPD를 이용한 분자생물학적 동정의 유용성)

  • Kim, Young-Kwon;Hong, Sung-Rho;Kim, Sang-Ha;Seo, Choong-Wonand
    • Korean Journal of Clinical Laboratory Science
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    • v.41 no.4
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    • pp.158-166
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    • 2009
  • On the basis of morphological characteristics, of total 128 strains of from sputum of tuberculos inpatient were identified as A. fumigatus (61 strains), A. niger (37), A. flavus (26), A. versicolor (1), A. nidulans (1), A. clavatus (1) and Neosartorya fennelliae (1). These strains were re-identified according to recent Aspergillus classification system which is mainly based on molecular characteristics. The strains were grouped by randomly amplified polymorphic DNA (RAPD) techniques. The representative strains from each group were sequenced with partial ${\beta}$-tubulin gene and compared with those of reference strains in the Aspergillus and were identified by the sequence. The identification was confirmed by morphology examination. As the results, they are reidentified as A. fumigatus (58), A. niger (11), A. tubingensis (26), A. flavus (27), A. sydowii (3), A. nidulans (1), A. clavatus (1) and Neosatorya fennelliae (1). This is the first report of A. tubuingensis in clinical field in Korea.

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Trajectory analysis of a CubeSat mission for the inspection of an orbiting vehicle

  • Corpino, Sabrina;Stesina, Fabrizio;Calvi, Daniele;Guerra, Luca
    • Advances in aircraft and spacecraft science
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    • v.7 no.3
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    • pp.271-290
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    • 2020
  • The paper describes the analysis of deployment strategies and trajectories design suitable for executing the inspection of an operative spacecraft in orbit through re-usable CubeSats. Similar missions have been though indeed, and one mission recently flew from the International Space Station. However, it is important to underline that the inspection of an operative spacecraft in orbit features some peculiar characteristics which have not been demonstrated by any mission flown to date. The most critical aspects of the CubeSat inspection mission stem from safety issues and technology availability in the following areas: trajectory design and motion control of the inspector relative to the target, communications architecture, deployment and retrieval of the inspector, and observation needs. The objectives of the present study are 1) the identification of requirements applicable to the deployment of a nanosatellite from the mother-craft, which is also the subject of the inspection, and 2) the identification of solutions for the trajectories to be flown along the mission phases. The mission for the in-situ observation of Space Rider is proposed as reference case, but the conclusions are applicable to other targets such as the ISS, and they might also be useful for missions targeted at debris inspection.

Neuroscience and the Social Powers of Narrative: How Stories Configure Our Brains

  • Armstrong, Paul B.
    • Journal of English Language & Literature
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    • v.64 no.1
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    • pp.3-24
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    • 2018
  • Stories are important instruments for configuring our cognitive and social worlds, but they do not necessarily make us more caring or less aggressive and self-involved. The ability to tell and follow a story requires cognitive capacities that are basic to the neurobiology of mental functioning, and so it would stand to reason that our experiences with stories would draw on and re-shape patterns of interaction that extend beyond the immediate experience of reading or listening to a narrative. Our intuitive, bodily-based ability to understand the actions of other people is fundamental to social relations, including the circuit between the representation of a configured action emplotted in a narrative and the reader's or listener's activity of following the story as we assimilate its patterns into the figures that shape our worlds. The activity of following a narrative can have a variety of beneficial or potentially noxious social consequences, either promoting the shared intentionality that neurobiologically oriented cultural anthropologists identify as a unique human capacity supporting culturally productive collaboration, or habitualizing and thereby naturalizing particular patterns of perception into rigid ideological constructs. The doubling of "me" and "not-me" in narrative acts of identification may promote the "we-intentionality" that makes socially beneficial cooperation possible, or it can set off mimetic conflict and various contagion effects. Neuroscience cannot predict what the social consequences of narrative will be, but it can identify the brain- and body-based processes through which (for better or worse) stories exercise social power.

Detection of Listeria Species by Conventional Culture-Dependent and Alternative Rapid Detection Methods in Retail Ready-to-Eat Foods in Turkey

  • Emine Dincer
    • Journal of Microbiology and Biotechnology
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    • v.34 no.2
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    • pp.349-357
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    • 2024
  • Foodborne pathogens, like Listeria monocytogenes, continue to inflict substantial financial losses on the food industry. Various methods for detecting Listeria in food have been developed and numerous studies have been conducted to compare the different methods. But, in recent years, new Listeria species have been identified, and currently the genus comprises 26 species. Therefore, it would be a more accurate approach to re-evaluate existing detection methods by considering new species. The present investigation involved the analysis of 42 ready-to-eat (RTE) foods, encompassing a variety of food categories, such as mezes, salads, dairy products, and meat products, with the aim of ascertaining the presence of Listeria. Among the traditional culture-dependent reference methods, the ISO 11290 method was preferred. The process of strain identification was conducted with the API Identification System. Furthermore, to ascertain the existence of L. monocytogenes and Listeria spp., the samples underwent additional analysis employing the VIDAS Immunoassay System, ELISA, and RT-PCR methodologies. Thus, four alternative approaches were employed in this study to compare not only the different methods used to determine Listeria while taking into account the newly identified Listeria species, but also to assess the compliance of retail RTE food items with microbiological criteria pertaining to the genus Listeria. Based on the conducted analyses, L. monocytogenes was conclusively determined to be present in one sample. The presence of Listeria spp. was detected in 30.9% of the samples, specifically in Turkish cig kofte, sliced salami, and salads.

Isolation and Identification of α-Glucosidase Inhibitory Compounds, Hyperoside, and Isoquercetin from Eleutherococcus senticosus Leaves (가시오갈피(Eleutherococcus senticosus) 잎으로부터 α-Glucosidase의 저해 활성 물질, Hyperoside와 Isoquercetin의 분리 및 구조·동정)

  • Lee, Ki Yeon;Hong, Soo Young;Jeong, Hye Jeong;Lee, Jae Hyoung;Lim, Sang Hyun;Heo, Nam-Kee;Kim, Songmun;Kim, Hee-Yeon
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.43 no.12
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    • pp.1858-1864
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    • 2014
  • In the present investigation, the anti-diabetic potential of 80% ethanol extract of Eleutherococcus senticosus leaves (EEES) was examined based on ${\alpha}$-glucosidase inhibitory activities. EEES was sequentially fractionated with n-hexane, chloroform, ethyl acetate (EtAOc), n-butanol, and $H_2O$. Of the various fractions, EtAOc fraction effectively inhibited ${\alpha}$-glucosidase activity by 68.05%. Therefore, EtAOc fraction was selected for further isolation and identification studies. EtAOc fraction was separated by medium pressure liquid chromatography with silica and ODS gel to yield eight fractions (EAA~EAH). Based on the results of ${\alpha}$-glucosidase inhibitory activity, EAH fraction was re-chromatographed to yielded four more fractions (EAHA~EAHD). Of these, EAHC fraction showed higher ${\alpha}$-glucosidase inhibitory activity of 93.60%. EAHC fraction was re-chromatographed and yielded EAHCA and EAHCB fractions. Further, identification and chemical structures of these two fractions were analyzed using $^1H$-NMR, $^{13}C$-NMR, and mass spectra data. Based on the results of the spectral data, the isolated compounds were identified as hyperoside and isoquercetin. Results of the present study indicate that the isolated compounds, hyperoside, and isoquercetin from leaves of E. senticosus could be used for the development of new anti-diabetic drugs.

Identification of Saponin and Sapogenin in Root, Leaf and Stem of Ginseng by Thin Layer Chromatography (얇은막 크로마토그래피에 의한 인삼(人蔘)의 근(根) 엽(葉) 및 경(莖)의 saponin 및 sapogenin화합물(化合物) 동정(同定))

  • Choi, Kang-Ju;Kim, Seok-Chang;Kim, Man-Wook;Nam, Ki-Yeul
    • Applied Biological Chemistry
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    • v.30 no.4
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    • pp.340-344
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    • 1987
  • Saponins of ginseng root, leaf and stem were identified by TLC. Eleven unknown spots were detected in ginseng leaf and ten unknown spots in ginseng stem on TLC besides seven ginsenosides such as $ginsenoside-Rg_1,\;-Rf,\;-Re,\;-Rd,\;-Rc,\;-Rb_2,\;and\;-Rb_1$ which are contained in ginseng root. $Ginsenoside-Rg_3\;and\;-Rg_2$ were identified on TLC from mild hydrolysates with 50% acetic acid of total saponins from ginseng root, leaf and stem. Meanwhile, panaxadiol, panaxatriol and oleanolic acid were identified from hydrolysates with 7% ethanolic sulfuric acid of total saponin of ginseng root, while panaxadiol and panaxatriol from those of total saponins of ginseng leaf and stem.

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Quantitative Trait Locus Mapping and Candidate Gene Analysis for Plant Architecture Traits Using Whole Genome Re-Sequencing in Rice

  • Lim, Jung-Hyun;Yang, Hyun-Jung;Jung, Ki-Hong;Yoo, Soo-Cheul;Paek, Nam-Chon
    • Molecules and Cells
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    • v.37 no.2
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    • pp.149-160
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    • 2014
  • Plant breeders have focused on improving plant architecture as an effective means to increase crop yield. Here, we identify the main-effect quantitative trait loci (QTLs) for plant shape-related traits in rice (Oryza sativa) and find candidate genes by applying whole genome re-sequencing of two parental cultivars using next-generation sequencing. To identify QTLs influencing plant shape, we analyzed six traits: plant height, tiller number, panicle diameter, panicle length, flag leaf length, and flag leaf width. We performed QTL analysis with 178 $F_7$ recombinant inbred lines (RILs) from a cross of japonica rice line 'SNU-SG1' and indica rice line 'Milyang23'. Using 131 molecular markers, including 28 insertion/deletion markers, we identified 11 main- and 16 minor-effect QTLs for the six traits with a threshold LOD value > 2.8. Our sequence analysis identified fifty-four candidate genes for the main-effect QTLs. By further comparison of coding sequences and meta-expression profiles between japonica and indica rice varieties, we finally chose 15 strong candidate genes for the 11 main-effect QTLs. Our study shows that the whole-genome sequence data substantially enhanced the efficiency of polymorphic marker development for QTL fine-mapping and the identification of possible candidate genes. This yields useful genetic resources for breeding high-yielding rice cultivars with improved plant architecture.

Use Case Identification Method based on Goal oriented Requirements Engineering(GoRE) (Goal 지향 요구공학 기반의 유스케이스 식별 방법)

  • Park, Bokyung;Kim, R. Youngchul
    • KIPS Transactions on Software and Data Engineering
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    • v.3 no.7
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    • pp.255-262
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    • 2014
  • Our previous research[1] suggested object extraction and modeling method based on Fillmore's case grammar. This approach had not considered of use case extraction and method. To solve this problem, we adopt Fillmore's semantic method as linguistic approach into requirement engineering, which refine fillmore's case grammar for extracting and modeling use cases from customer requirements. This Refined mechanism includes the definition of a structured procedure and the representation of visual notations for 'case' modeling. This paper also proposes the use case decision matrix to identify use case size from extracted use cases based on goal oriented requirement engineering(GoRE), which related with the complexity of use case, and also prioritizes the use cases with this matrix. It demonstrates our proposal with the bank ATM system.

Comparative Analysis of Metabolites in Roots of Panax ginseng Obtained from Different Sowing Methods (파종 방법에 따른 고려인삼의 대사체 비교)

  • Yang, Seung Ok;Lee, Sung Woo;Kim, Young Ock;Lee, Sang Won;Kim, Na Hyun;Choi, Hyung Kyoon;Jung, Joo Yeoun;Lee, Dong Ho;Shin, Yu Su
    • Korean Journal of Medicinal Crop Science
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    • v.22 no.1
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    • pp.17-22
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    • 2014
  • Ginsenosides of roots in Panax ginseng were analyzed by metabolic-targeting HPLC using the partial least squares discriminant analysis (PLS-DA) and compared depending on sowing methods between direct seeding and transplanting method. Score plots derived from PLS-DA could identify the sowing method between the direct seeding and transplanting method in P. ginseng roots. The ginsenoside compounds were assigned as Rg1, Re, Rf, Rg2, Rb1, Rc, Rb2, Rb3, and Rd. Contents of Re, Rf, Rg2, Rb1, Rc, Rb3, and Rd of main roots produced from the transplanting method were relatively higher than those of samples produced from direct seeding method. Also, contents of Rg1, Re, Rf, Rg2, Rb1, Rc, Rb2, Rb3, and Rd of lateral roots from the transplanted samples were relatively higher than those of samples produced from direct seeding method. Therefore, HPLC with PLS-DA analysis can be a straightforward tool for identification of ginsenosides in main or lateral roots of P. ginseng obtained from two different seeding methods between direct and transplanting methods.

Adaptive length SMA pendulum smart tuned mass damper performance in the presence of real time primary system stiffness change

  • Contreras, Michael T.;Pasala, Dharma Theja Reddy;Nagarajaiah, Satish
    • Smart Structures and Systems
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    • v.13 no.2
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    • pp.219-233
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    • 2014
  • In a companion paper, Pasala and Nagarajaiah analytically and experimentally validate the Adaptive Length Pendulum Smart Tuned Mass Damper (ALP-STMD) on a primary structure (2 story steel structure) whose frequencies are time invariant (Pasala and Nagarajaiah 2012). In this paper, the ALP-STMD effectiveness on a primary structure whose frequencies are time varying is studied experimentally. This study experimentally validates the ability of an ALP-STMD to adequately control a structural system in the presence of real time changes in primary stiffness that are detected by a real time observer based system identification. The experiments implement the newly developed Adaptive Length Pendulum Smart Tuned Mass Damper (ALP-STMD) which was first introduced and developed by Nagarajaiah (2009), Nagarajaiah and Pasala (2010) and Nagarajaiah et al. (2010). The ALP-STMD employs a mass pendulum of variable length which can be tuned in real time to the parameters of the system using sensor feedback. The tuning action is made possible by applying a current to a shape memory alloy wire changing the effective length that supports the damper mass assembly in real time. Once a stiffness change in the structural system is detected by an open loop observer, the ALP-STMD is re-tuned to the modified system parameters which successfully reduce the response of the primary system. Significant performance improvement is illustrated for the stiffness modified system, which undergoes the re-tuning adaptation, when compared to the stiffness modified system without adaptive re-tuning.