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http://dx.doi.org/10.7783/KJMCS.2014.22.1.17

Comparative Analysis of Metabolites in Roots of Panax ginseng Obtained from Different Sowing Methods  

Yang, Seung Ok (Department of Medicinal Crop Research, NIHHS, Rural Development Administration)
Lee, Sung Woo (Department of Medicinal Crop Research, NIHHS, Rural Development Administration)
Kim, Young Ock (Department of Medicinal Crop Research, NIHHS, Rural Development Administration)
Lee, Sang Won (Department of Medicinal Crop Research, NIHHS, Rural Development Administration)
Kim, Na Hyun (Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University)
Choi, Hyung Kyoon (College of Pharmacy, Chung-Ang University)
Jung, Joo Yeoun (Department of Food Science and Technology, Oregon State University)
Lee, Dong Ho (Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University)
Shin, Yu Su (Department of Medicinal Crop Research, NIHHS, Rural Development Administration)
Publication Information
Korean Journal of Medicinal Crop Science / v.22, no.1, 2014 , pp. 17-22 More about this Journal
Abstract
Ginsenosides of roots in Panax ginseng were analyzed by metabolic-targeting HPLC using the partial least squares discriminant analysis (PLS-DA) and compared depending on sowing methods between direct seeding and transplanting method. Score plots derived from PLS-DA could identify the sowing method between the direct seeding and transplanting method in P. ginseng roots. The ginsenoside compounds were assigned as Rg1, Re, Rf, Rg2, Rb1, Rc, Rb2, Rb3, and Rd. Contents of Re, Rf, Rg2, Rb1, Rc, Rb3, and Rd of main roots produced from the transplanting method were relatively higher than those of samples produced from direct seeding method. Also, contents of Rg1, Re, Rf, Rg2, Rb1, Rc, Rb2, Rb3, and Rd of lateral roots from the transplanted samples were relatively higher than those of samples produced from direct seeding method. Therefore, HPLC with PLS-DA analysis can be a straightforward tool for identification of ginsenosides in main or lateral roots of P. ginseng obtained from two different seeding methods between direct and transplanting methods.
Keywords
Panax ginseng Root; Sowing Method; Metabolic Targeting; HPLC;
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Times Cited By KSCI : 8  (Citation Analysis)
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