Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Comparative Analysis of Metabolites in Roots of Panax ginseng Obtained from Different Sowing Methods

파종 방법에 따른 고려인삼의 대사체 비교

  • Yang, Seung Ok (Department of Medicinal Crop Research, NIHHS, Rural Development Administration) ;
  • Lee, Sung Woo (Department of Medicinal Crop Research, NIHHS, Rural Development Administration) ;
  • Kim, Young Ock (Department of Medicinal Crop Research, NIHHS, Rural Development Administration) ;
  • Lee, Sang Won (Department of Medicinal Crop Research, NIHHS, Rural Development Administration) ;
  • Kim, Na Hyun (Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University) ;
  • Choi, Hyung Kyoon (College of Pharmacy, Chung-Ang University) ;
  • Jung, Joo Yeoun (Department of Food Science and Technology, Oregon State University) ;
  • Lee, Dong Ho (Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University) ;
  • Shin, Yu Su (Department of Medicinal Crop Research, NIHHS, Rural Development Administration)
  • 양승옥 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 이성우 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김영옥 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 이상원 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김나현 (고려대학교 생명과학대학 생명공학부) ;
  • 최형균 (중앙대학교 약학대학) ;
  • 정주연 (오레곤 주립대 식품공학과) ;
  • 이동호 (고려대학교 생명과학대학 생명공학부) ;
  • 신유수 (농촌진흥청 국립원예특작과학원 인삼특작부)
  • Received : 2013.10.23
  • Accepted : 2014.01.14
  • Published : 2014.02.28
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Abstract

Ginsenosides of roots in Panax ginseng were analyzed by metabolic-targeting HPLC using the partial least squares discriminant analysis (PLS-DA) and compared depending on sowing methods between direct seeding and transplanting method. Score plots derived from PLS-DA could identify the sowing method between the direct seeding and transplanting method in P. ginseng roots. The ginsenoside compounds were assigned as Rg1, Re, Rf, Rg2, Rb1, Rc, Rb2, Rb3, and Rd. Contents of Re, Rf, Rg2, Rb1, Rc, Rb3, and Rd of main roots produced from the transplanting method were relatively higher than those of samples produced from direct seeding method. Also, contents of Rg1, Re, Rf, Rg2, Rb1, Rc, Rb2, Rb3, and Rd of lateral roots from the transplanted samples were relatively higher than those of samples produced from direct seeding method. Therefore, HPLC with PLS-DA analysis can be a straightforward tool for identification of ginsenosides in main or lateral roots of P. ginseng obtained from two different seeding methods between direct and transplanting methods.

Keywords

References

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