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http://dx.doi.org/10.3746/jkfn.2014.43.12.1858

Isolation and Identification of α-Glucosidase Inhibitory Compounds, Hyperoside, and Isoquercetin from Eleutherococcus senticosus Leaves  

Lee, Ki Yeon (Gangwondo Provincial Agricultural Research & Extension Services)
Hong, Soo Young (Gangwondo Provincial Agricultural Research & Extension Services)
Jeong, Hye Jeong (Gangwondo Provincial Agricultural Research & Extension Services)
Lee, Jae Hyoung (Gangwondo Provincial Agricultural Research & Extension Services)
Lim, Sang Hyun (Gangwondo Provincial Agricultural Research & Extension Services)
Heo, Nam-Kee (Gangwondo Provincial Agricultural Research & Extension Services)
Kim, Songmun (Department of Bioconvergence Science and Technology, Kangwon National University)
Kim, Hee-Yeon (Gangwondo Provincial Agricultural Research & Extension Services)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.43, no.12, 2014 , pp. 1858-1864 More about this Journal
Abstract
In the present investigation, the anti-diabetic potential of 80% ethanol extract of Eleutherococcus senticosus leaves (EEES) was examined based on ${\alpha}$-glucosidase inhibitory activities. EEES was sequentially fractionated with n-hexane, chloroform, ethyl acetate (EtAOc), n-butanol, and $H_2O$. Of the various fractions, EtAOc fraction effectively inhibited ${\alpha}$-glucosidase activity by 68.05%. Therefore, EtAOc fraction was selected for further isolation and identification studies. EtAOc fraction was separated by medium pressure liquid chromatography with silica and ODS gel to yield eight fractions (EAA~EAH). Based on the results of ${\alpha}$-glucosidase inhibitory activity, EAH fraction was re-chromatographed to yielded four more fractions (EAHA~EAHD). Of these, EAHC fraction showed higher ${\alpha}$-glucosidase inhibitory activity of 93.60%. EAHC fraction was re-chromatographed and yielded EAHCA and EAHCB fractions. Further, identification and chemical structures of these two fractions were analyzed using $^1H$-NMR, $^{13}C$-NMR, and mass spectra data. Based on the results of the spectral data, the isolated compounds were identified as hyperoside and isoquercetin. Results of the present study indicate that the isolated compounds, hyperoside, and isoquercetin from leaves of E. senticosus could be used for the development of new anti-diabetic drugs.
Keywords
Eleutherococcus senticosus; ${\alpha}$-glucosidase inhibitor; hyperoside; isoquercetin;
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Times Cited By KSCI : 13  (Citation Analysis)
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1 Lim SJ, Kim SH. 2001. The effect of each fraction of methanol extract of Alisma canaliculatum on blood glucose levels and lipid metabolism in streptozotocin induced diabetic rats. Korean J Nutr 34: 619-625.   과학기술학회마을
2 Laakso M, Cederberg H. 2012. Glucose control in diabetes: which target level to aim for? J Intern Med 272: 1-12.   DOI   ScienceOn
3 Lee BB, Park SR, Han CS, Han DY, Park EJ, Lee SC. 2008. Antioxidant activity and inhibition activity against ${\alpha}$-amylase and ${\alpha}$-glucosidase of Viola mandshurica extracts. J Korean Soc Food Sci Nutr 37: 405-409.   과학기술학회마을   DOI
4 Yoshio K. 2005. Obesity and related diseases. Koonja Publishing Company, Seoul, Korea. p 691-692.
5 Oh HC, Kim DH, Cho JH, Kim YC. 2004. Hepato-protective and free radical scavenging activities of phenolics petrosins and flavonoids isolated from Equisetum arvense. J Ethnopharmacol 95: 421-424.   DOI
6 Choi HJ, Jeong YK, Kang DO, Joo WH. 2008. Inhibitory effects of four solvent fractions of Alnus firma on ${\alpha}$-amylase and ${\alpha}$-glucosidase. J Life Sci 18: 1005-1010.   과학기술학회마을   DOI   ScienceOn
7 Kim SK, Kim Y, Park MS, Chio SY, Yun SJ. 1998. Intraspecific relation of E. senticodsus Max. by RAPD markers. Korean J Med Crop Sci 6: 165-169.
8 Jin LH, Han SS, Choi YS. 2002. Antioxidant effect of the extracts of Acanthopanax senticosus. Korean J Pharmacogn 33: 359-363.
9 Brekhman II, Dardymov IV. 1969. New substance of plant origin which increase nonspecific resistance. Annu Rev Pharmacol 9: 419-430.   DOI   ScienceOn
10 Lee BB, Park SR, Han CS, Han DY, Park EJ, Lee SC. 2008. Antioxidant activity and inhibition activity against ${\alpha}$-amylase and ${\alpha}$-glucosidase of Viola mandshurica extracts. J Korean Soc Food Sci Nutr 37: 405-409.   과학기술학회마을   DOI
11 Lim SH, Park YH, Kwon CJ, Ham HJ, Jeong HN, Kim KH, Ahn YS. 2010. Anti-diabetic and hypoglycemic effect of Eleutherococcus spp. J Korean Soc Food Sci Nutr 39: 1761-1768.   과학기술학회마을   DOI
12 Lee JM, Kim HM, Lee SL, Han S, Cho SH, Lee SH. 2010. Determination of hyperin in the fruits of Ancanthopanax species by performance lipid chromatography. Nat Prod Sci 16: 39-42.
13 Kim SG, Choi JW, Park HJ, Lee SM, Jung HJ. 2009. Antihyperlipidemic effect of the flavonoid-rich fraction from the methanol extract of Orostachy japonicus in rats. Korean J Pharmacogn 40: 51-58.
14 Choe KH, Choe SN, Choo JJ, Lee JY, Kim JY, Kim JW, Choi JS, Park KS, Park KH. 2007. Tissue concentration of quercetin, isoquercetin and hyperoside, and lipid profile changes following 8-week feeding of Angelica keiskei power in rats. Korean J Food Sci Technol 39: 721-724.
15 Lee SH, Chung HS, Shin KH, Kim BK. 2004. Determination of hyperin in Ancanthopanax sesticosus and A. sessiliflorus by HPLC. Yakhak Hoeji 48: 231-235.
16 Chen M, Song F, Guo M, Liu Z, Liu S. 2002. Analysis of flavonoid constituents from leaves of Acanthopanax sesticosus Harms by electrospray tandem mass spectrometry. Rapid Commun Mass Spectrum 16: 264-271.   DOI   ScienceOn
17 Tang W, Eisenbrand G. 1992. Chinese dugs of plant origin. Springer Publishing Company, Berlin, Germany. p 1-12.
18 Hwang JY, Han JS. 2007. Inhibitory effects of Sasa borealis leaves extracts on carbohydrate digestive enzymes and postprandial hyperglycemia. J Korean Soc Food Sci Nutr 36: 989-994.   과학기술학회마을   DOI
19 Lee YS, Jung SH, Lim SS, Ji J, Lee SH, Shin KH. 2001. Effects of the water extract from the stem bark of Acanthopanax senticosus on hyperlipidemia in rats. Korean J Pharmacogn 32: 103-107.   과학기술학회마을
20 Brekhman II. 1969. A new medicinal plant of the family Araliaceae the spiny Eleutherococcus. Izv Sibir Otdel Akad Nauk USSR 9: 113-120.
21 Liu HS, Liu IM, Cheng JT, Lin CL, Hsu FL. 2008. Hypoglycemic effect of syringin from Eleutherococcucs senticosus in streptozotocin-induced diabetic rats. Planta Med 74: 109-113.   DOI   ScienceOn
22 Nishibe S, Kinoshita H, Takeda H, Okano G. 1990. Phenolic compounds from stem bark of Acanthopanax sentocosus and their pharmacological effect in chronic swimming stressed rats. Chem Pharm Bull 38: 1763-1765.   DOI   ScienceOn
23 Yamazaki T, Shimosaka S, Sasaki H, Matsumura T, Tukiyama T, Tokiwa T. 2007. (+)-Syringaresinol-di-O-${\beta}$-d-glucoside, a phenolic compound from Acantopanax senticosus Harms, suppresses proinflammatory mediator in SW982 human synovial sarcoma cells by inhibiting activating protein-1 and/or nuclear factor-${\kappa}B$ activities. Toxicol In Vitro 21: 1530-1537.   DOI   ScienceOn
24 Bu Y, Jin ZH, Park SY. 2005. Siberian ginseng reduces infarct volume in transient focal cerebral ischaemia in Sprague-Dawley rats. Phyto Res 19: 167-169.   DOI   ScienceOn
25 Ahn JY, Um MY, Lee HJ, Jung CH, Heo SH, Ha TY. 2013. Eleutheroside E, an active component of Eleutherococcus senticosus, ameliorates insulin resistance in type 2 diabetic db/db mice. Evid Based Complement Alternat Med Article ID 934183.
26 Yean MH, Kim JS, Hyun YJ, Hyun JW, Bae KH, Kang SS. 2012. Terpenoids and phenolics from Geum japonicum. Korean J Pharmacogn 43: 107-121.   과학기술학회마을
27 Appleton J. 2010. Evaluating the bioavailability of isoquercetin. Nat Med J 2: 1-6.
28 Park JC, Kim SH. 1995. Flavonoid analysis from the leaves of Eucommia ulmoides. J Korean Soc Food Nutr 24: 901-905.   과학기술학회마을
29 Lee YJ, Kim EO, Choi SW. 2011. Isolation and identification of antioxidant polyphenolic compounds in mulberry (Morus alba L.) seeds. J Korean Soc Food Sci Nutr 40: 517-524.   과학기술학회마을   DOI   ScienceOn
30 Kim HY, Moom BH, Lee HK, Choi DH. 2004. Flavonoid glycosides from the leaves of Eucommia ulmoides O. with glycation inhibitory activity. J Ethnopharmacol 93: 227-230.   DOI   ScienceOn
31 Kim YS. 2005. Antimicrobial activity of yacon K-23 and manufacture of functional yacon jam. Korean J Food Sci Technol 37: 1035-1038.   과학기술학회마을
32 Zhang R, Yao Y, Wang Y, Ren G. 2011. Antidiabetic activity of isoquercetin in diabetic KK-$A^y$ mice. Nutr Metab 8: 85.   DOI