• The Plant Pathology Journal
• /
• 제15권3호
• /
• pp.137-143
• /
• 1999

A novel chromosomal gene tagging technique using a specific fragment of the fatty acid desaturase-like open reading frame (des-like ORF) from the tox-argK gene cluster of Pseudomonas syringae pv. phaseolicola was developed to identify Xanthomonas spp.released into the environment as biocontrol agents. X. campestris pv. convolvuli FB-635, a pathogen of Convolvulus arvensis L., (bindweed), was chosen as the organism in which to develop and test the system. A 0.52 kb DES fragment amplified from P. syringae pv. phaseolicola C-199 was inserted into pGX15, a cosmid clone containing a 10.3 kb Eco RI-HindIII fragment derived from the xanthomonadin biosynthetic gene cluster contained in plasmid pIG102, to create a pigG::DES insertion. The 10.8 kb EcoRI-BamHI fragment carrying the pigG:: DES insertion was cloned into pLAFR3 to generate pLXP22. pLXP22 was then conjugated into X. campestris pv. convolvuli FB-635 and the pigG::DES insertion integrated into the bacterial chromosome by marker exchange. Rifampicin resistant, tetracycline sensitive, starch hydrolyzing, white colonies were used to differentiate the marked strain from yellow pigmented wild-type ones. PCR primers specific for the unique DES fragment were used for direct detection of the marked strain. Result showed the marked strain could be detected at very low levels even in the presence of high levels of other closely related or competitive bacteria. This PCR-based DES-tagging system provides a rapid and specific tool for directly monitoring the dispersal and persistence of Xanthomonas spp.released into the environment.

• 정보처리학회논문지D
• /
• 제12D권3호
• /
• pp.335-344
• /
• 2005

근래들어 구성 요소가 빠른 속도로 지속적으로 발생되는 무한 집합으로 정의되는 데이터 스트림에 대한 개방 데이터 마이닝 방법들이 활발히 제안되고 있다. 데이터 스트림에 내재된 정보들은 시간 흐름에 따른 변화의 가능성이 매우 높다. 따라서, 이러한 변화를 빠른 시간에 분석할 수 있다면 해당 데이터 스트림에 대한 분석에서 보다 유용한 정보를 제공할 수 있다. 본 논문에서는 개방 데이터 마이닝 환경에서 효율적인 최근 빈발 항목 탐색을 위한 이동 윈도우 기법을 제시한다. 해당 기법에서는 데이터 스트림이 지속적으로 확장되더라도 지연 추가 및 전지 작업을 적용하여 마이닝 수행과정에서의 메모리 사용량이 매우 작게 유지되며, 분석 대상 범위의 데이터 객체들을 반복적으로 탐색하지 않기 때문에 각 시점에서 마이닝 결과를 짧은 시간에 구할 수 있다. 더불어, 해당 방법은 데이터 스트림의 최근 정보에 집중한 분석을 통해 해당 데이터 집합의 변화를 효율적으로 감지할 수 있다.

• 한국정보통신학회논문지
• /
• 제8권8호
• /
• pp.1828-1834
• /
• 2004

무선 통신 및 모바일 기술의 급속한 발전으로 인해 위치 기반 서비스 및 모바일 응용 서비스와 같은 이동 객체에 기반한 다양한 서비스에 대한 요구가 증가하게 되었다. 이를 위해, 본 논문에서는 모바일 응용에서 이동 객체에 대한 다양한 질의 중에서 특히 궤적-기반 질의에 대한 성능을 향상시킬 수 있는 색인 구조를 제안한다. 제안하는 색인 구조는 이동 객체의 궤적-기반 질의를 위해 제안되었던 기존의 TB-tree의 삽입 및 검색 성능의 효율성을 높이기 위해 연결테이블(LinkTable:L-Table)을 유지한다. L-Table은 선행 노드를 한번에 직접 접근하기 위해 이동 객체의 처음 세그먼트와 마지막 세그먼트가 저장된 단말 노드를 가리키는 포인터 정보와 디스크에서의 페이지를 가리키는 페이지 번호를 저장한다. 아울러 시스템의 가용메모리가 제약을 받는 경우에도 일정한 크기의 버퍼를 유지해 색인의 일부만을 메모리에 상주시킬 수 있도록 설계한다. 마지막으로 제안하는 색인 구조의 성능을 평가하기 위하여 다양한 실험 데이타를 이용해 성능 평가를 수행한 결과, 삽입 및 궤적-기반 질의 측면에서 기존의 색인 구조들에 비해 더 나은 성능을 보인다.

• 대한전자공학회논문지SP
• /
• 제45권4호
• /
• pp.1-9
• /
• 2008

본 논문은 DC 영상을 이용하여 기하학적 변환에 강인하며 고속처리가 가능한 워터마킹 알고리즘을 제안한다. 제안하는 방법은 JPEG 및 MPEG 영상압축 기술에 널리 사용되는 $8{\times}8$ 블록 DCT를 기반으로 하며, DC 성분만으로 구성된 DC 영상에 DWT 수행 후 웨이블릿 계수에 워터마크를 삽입한다. DC 성분을 이용한 워터마크 삽입으로 야기될 수 있는 화질 열화를 극복하기 위하여 DWT의 서브밴드 마다 워터마크 삽입 강도와 삽입량을 차별화한다. 제안하는 알고리즘은 $8{\times}8$ 블록 DCT 영역의 부분적인 복호화(즉, DC 영상)를 수행함으로써 실시간 워터마크 삽입 및 추출을 위한 고속처리가 가능하다. 실험결과로부터 제안한 워터마킹 방법이 기존의 DC 성분을 이용한 알고리즘과 비교하여 82%의 계산시간을 단축하였으며 다양한 기하학적 공격과 JPEG 압축 등의 기타 공격에 강인한 것을 확인하였다.

• 대한기계학회논문집
• /
• 제17권10호
• /
• pp.2563-2573
• /
• 1993

The measurements of monochromatic line-of-sight flame emission and light transmission in the same path having small spatial resolution were performed in an axisymmetric laminar propane $C_{3}H_{8}$ diffusion flame. The light wavelengthes of 632 nm, 800nm, 900nm were used. From these measurements, local point soot radiances (by Kirchhoff's law) and absorption coefficients were reconstructed by tomography. Thus local point soot temperatures and concentrations were obtained. The reconstructed soot temperatures and concentrations of local points have no differences between the case of visible range (632 nm) and the case of infrared range (800 nm and 900 nm). In these ranges, the scattering coefficient is much lower than the absorption coefficient. Soot mean temperature over the path also matches well with local soot temperature in outer region of the flame. Temperature measurement by thermocouple with different bead diameters $(222{\mu}m and 308{\mu}m)$ was carried in the same flame. Rapid insertion technique was used and radiation effect was considered. Radiation correction in the sooting region was carried out and the corrected result was in good agreement with the local soot temperature.

• 대한전자공학회논문지SP
• /
• 제41권5호
• /
• pp.203-216
• /
• 2004

최근의 영상 압축기술 발달과 이동통신의 급속한 성장으로 고품질의 멀티미디어 서비스를 이동통신과 같은 에러에 노출된 채널 환경 하에서 제공하는 것이 점차로 가능해짐에 따라 이를 뒷받침할 에러 강인 기술에 대한 요구가 커졌다. 본 논문에서는 H.324M 환경에서 MPEG-4 심플 프로파일과 IP 패킷 네트워크 환경에서 H.264/AVC 베이스라인 기술의 성능을 분석하고 평가한다. 특히, MPEG-4 심플 프로파일의 재동기화 기술과 데이터 구분화 기술, 그리고 H.264/AVC 베이스라인의 유연한 매크로블록 순서(FMO) 기술을 여러 산발(Random) 비트와 군집(Burst) 비트에러 조건하에서 복호화된 영상의 객관적인 화질과 압축률 측면에서 평가한다.

• 한국임상수의학회지
• /
• 제16권2호
• /
• pp.300-308
• /
• 1999

Arthroscopy is a valuable diagnostic and operative tool in equine and human orthopedics. The arthroscope is a difficult instrument to use and requires patience, practice, and persistence in order to obtain good results. This technique was found to be less traumatic than arthrotomy, invasiveness, rapid recovery and the feasibility of surgically correcting many arthropathies. The use of the arthroscope in the dog provides a new dimension in the study and diagnosis of joint derangements. The purpose of this report is to introduce the use of the arthroscope in the dog-more specifically, in the stifle of the dog. A diameter 2.7 mm fore-oblique viewing arthroscope, cold light source, video and video printer are used. With the fore-oblique viewing type it is possible to view directly as well as slightly to the side and the range of viewing can be increased by rotating the arthroscope around the object The scope is connected with a cold light source by means of a fiber-optic light guide. The stifle joint was flexed to 20~30$^{\circ}$. The joint were lavaged with lactated Ringer's solution during arthroscopic examination. Arthroscopy of the stifle was performed prior to arthrotomy in 1 dead dog and 4 healthy dogs, and other 3 dogs was performed only arthroscopic examination. In this study only the conventional approaches were used and in most cases it was possible to view all the intra-articular structures via the lateral infrapatellar approach. In the stifle joint, endoscopic observation was performed to find lateral femoral condyle, patella, medial femoral condyle, trochlear groove, tibia, fat, cranial cruciate ligament, caudal cruciate ligament lateral meniscus, tendon of long digital extensor muscle, medial meniscus, and medial collateral ligament Post-arthroscopic examination, the lameness had disappeared within 12~24 hours. Pain and swelling in the stifle joint had disappeared within 24~36 hours. Post-arthroscopic secondary infection was never encountered in the dogs. In conclusion, arthroscopic insertion technique in canine stifle joint using a diameter 2.7 mm 30$^{\circ}$ arthroscope was established and arthroscopical views of all anatomical structures in the normal stilfe joint were obtained through lateral infrapatellar portal.

• 대한치과보철학회지
• /
• 제43권6호
• /
• pp.736-744
• /
• 2005

Statement of problem. Initial stability of implant is an important factor for predicting osseointegration. It requires a rapid, non-invasive, user-friendly technique to frequently assess the implant stability and the degree of osseointegration. Purpose. The aim of this study was to evaluate the correlation between the resonance frequency analysis (RFA) and the radiographic method for peri-implant bone change under in vitro conditions. Material and Method. Twenty implants of 3.75 mm in diameter(Neoplant, Neobiotech, Korea) were used. To simulate peri-implant bone change, 2 mm-deep $45^{\circ}$ range horizontal defect and 2 mm-deep $90^{\circ}$ range horizontal defect area were serially prepared perpendicular to the X-ray beam after conventional implant insertion. Customized film holding device was fabricated to standardize the projection geometry for serial radiographs of implants and direct digital image was obtained. ISQ values and gray values inside threads were measured before and after peri-implant bone defect preparation. Results. Within a limitation of this study, ISQ value of resonance frequency analysis was changed according to peri-implant bone change (p<0.05) and gray value of radiographic method was changed according to peri-implant bone change (p<0.05). There was no correlation between the ISQ value and the gray value for peri-implant bone change (p>0.05). But, in horizontal defect condition, relatively positive correlation were between ISQ and gray values(r=0.663). Conclusion. This results provided a possibility that peri-implant bone change may be evaluated by both RFA and radiographic method.

• 생명과학회지
• /
• 제23권7호
• /
• pp.955-961
• /
• 2013

유전공학은 대장균의 유전자조작으로부터 시작되어 지금은 생명과학전반에서 급속하게 발전하고 있다. 최근의 유전자조작기술은 특정유전자를 염색체 내에 넣고 빼고 하는 기술이 확립되어서 의학과 농학을 포함한 응용생명과학분야에서 유용하게 이용되고 있다. 본 리뷰에서는 높은 친환경성과 경제성을 가진 '지배적 치사곤충방사법'(Release of insects Carrying a Dominant Lethal: RIDL)을 중심으로 포괄적인 분자해충관리에 관하여 설명한다. 비록 현재로서는 RIDL을 직접 포장에 적용하기에는 아직 어려운 점이 많지만, 계속적인 유전공학기술의 발전을 통하여 농약을 사용하지 않고 생태계를 교란하지 않는 범위에서 사용될 수 있는 가장 좋은 해충관리방법 중의 한 가지가 될 것이다. 이때에 가장 고려할 점은 주위 환경교란부분에 많은 주의가 요구된다.

• Journal of Korean Neurosurgical Society
• /
• 제30권sup2호
• /
• pp.294-299
• /
• 2001

Objectives : CT-guided stereotactic evacuation for spontaneous intracerebral hemorrhage can minimize the brain damage and can be performed safely and simply under local anesthesia. But that procedure is time consuming and has a risk of rebleeding because of the stress during head pin fixation. So authors describe easy and precise guidelines for FHA of putaminal hemorrhage without stereotactic instrument. Methods and Materials : We analyzed the data of 298 patients who underwent CT-guided stereotactic aspiration of putaminal hematoma in our hospital between January 1990 and December 2000. We divided the patients into three groups according to the location of hematoma : anterior portion, middle portion and posterior portion of putamen. Total number of catheters inserted into the hematoma were 345 and there were with regard to the direction and depth of catheters. Results : Proposed guidelines of catheter insertion to putaminal hemorrhage in our institution. 1) hematoma at the anterior portion of putamen ; Direction of catheter was the midpupillary line of the eye and the point intersecting a line drawn from the burr hole to a point between external auditory meatus(EOM) and 1cm posterior to EOM. Depth of catheter was 6-6.5cm. 2) hematoma at the middle portion of putamen ; Direction of catheter was the midpupillary line of the the eye and the point intersecting a line drawn from the burr hole to a point between 1cm and 2cm posterior to EOM. Depth of catheter was 6.5-7cm. 3) hematoma at the posterior portion of putamen ; Direction of catheter was 15 degree laterally from the midpupillary line of the eye and the point intersecting a line drawn from the burr hole to a point between 2cm and 3cm posterior to EOM. Depth of catheter was 7-7.5cm. We have performed FHA of putaminal hemorrhage in 48 cases according to this guideline. All catheter were inserted exactly at the center of hematoma and average operation time was about 30 minutes. Conclusion : Our proposed guidelines for putaminal hemorrhage are considered to be safe and simple method with similar accuracy and rapid decompression compared with traditional stereotactic method. Main advantages of this technique were unnecessity of stereotactic frame application and less time requirement for hematoma removal.