• Molecules and Cells
• /
• 제40권4호
• /
• pp.271-279
• /
• 2017

Ran-binding protein family member, RanBP9 has been reported in various basic cellular mechanisms and neuropathological conditions including schizophrenia. Previous studies have reported that RanBP9 is highly expressed in the mammalian brain and retina; however, the role of RanBP9 in retinal development is largely unknown. Here, we present the novel and regulatory roles of RanBP9 in retinal development of a vertebrate animal model, zebrafish. Zebrafish embryos exhibited abundant expression of ranbp9 in developing brain tissues as well as in the developing retina. Yeast two-hybrid screening demonstrated the interaction of RanBP9 with Mind bomb, a component of Notch signaling involved in both neurogenesis and neural disease autism. The interaction is further substantiated by co-localization studies in cultured cells. Knockdown of ranbp9 resulted in retinal dysplasia with defective proliferation of retinal cells, downregulation of neuronal differentiation marker huC, elevation of neural proliferation marker her4, and alteration of cell cycle marker p57kip2. Expression of the $M{\ddot{u}}ller$ glial cell marker glutamine synthase was also affected in knockdown morphants. Our results suggest that Mind bomb-binding partner RanBP9 plays a role during retinal cell development of zebrafish embryogenesis.

• Toxicological Research
• /
• 제25권1호
• /
• pp.35-40
• /
• 2009

TCDD (2,3,7,8-tetrachlorodibenzo-p-dioxin) and related halogenated aromatic hydrocarbons elicit a diverse spectrum of biochemical and toxic responses in laboratory animals and mammalian cells in culture. Toxicity and carcinogenicity of TCDD is well established but the molecular mechanism is still poorly understood. Here, we found the noble responsive genes to TCDD using the differential display analysis. Treatment of HepG2 cells with TCDD showed a significantly different mRNA expression pattern from the untreated cells in differential display analysis. The differentially displayed bands were isolated and used as probes in dot blot and Northern blot analyses. Of thirty-five isolated differentially displayed bands, only two bands were confirmed as positive in dot blot and Northern blot analyses. The nucleotides sequences of these clones were analyzed and the search of Genebank database revealed that one clone is highly homologous with RanBP2 (Ras-related nuclear protein binding protein2; 92%) and the other is an unknown gene. RanBP2 is a nucleoporin with SUMO E3 ligase activity that functions in both nucleocytoplasmic transport and mitosis and its role as a novel tumor suppressor has been recently proposed. Thus, these results may suggest the clue elucidating the toxic mechanism of TCDD through RanBP2.

• Toxicological Research
• /
• 제21권2호
• /
• pp.175-178
• /
• 2005

Butyl $\rho$-hydroxybenzoic acid (butyl paraben, BP) is a homologous series of parabens and is widely used as a preservative in cosmetic and pharmaceutical products. The purpose of this study was to investigate the estrogenic/antiandrogenic activities of BP in animals. For that, we performed an uterotrophic assay and a Hershberger assay in rats. In uterotrophic assay, BP was administered subcutaneously to immature female SD rats (18 days old) for 3 consecutive days. The wet and dry uterus weights were significantly increased in the groups treated with BP in dose­dependent manner. In case of Hershberger assay, BP significantly reduced the weight of seminal vesicle of castrated rats. And other accessory organ/glands - prostate, Cowper's glands, bulbocavernosus muscle and glans penis were also slightly decreased. The results of this study suggested that BP showed estrogenic and anti-androgenic activities in vivo.

• 한국식품과학회지
• /
• 제44권1호
• /
• pp.48-54
• /
• 2012

본 연구에서는 강정의 포장방법 및 포장재질이 강정의 저장 중 품질특성에 미치는 영향을 조사하기 위해서 포장방법별로 개별포장, 대형포장 및 봉지포장의 3가지 방법과 8개의 포장재질을 사용하였다. 강정 포장은 (1) IP: OPP/PE+종이, (2) LP1: OPP/PE+종이, (3) LP2: OPP/PE+관, (4) LP3: OPP/PE+플라스틱, (5) BP1: OPP/PE, (6) BP2: OPP/DL/CPP, (7) BP3: PET/PE/CPP, (8) BP4: OPP/PVDC/PE/CPP로 구분하였다. 강정의 저장초기 지방산 조성은 linoleic acid(54.2%), oleic acid(24.1%), palmitic acid (11.2%) linoleic acid(6.0%) and stearic aicd(3.8%)이었으며, 저장중 수분함량, 수분활성도, 경도, 산가, 과산화물가를 측정하였다. 저장 중 수분함량의 변화가 늦게 나타난 시료는 LP2, BP4 > IP > LP3, BP1, BP2 > LP1 > BP3 순이었다. 수분활성도는 큰 차이를 보이지 않았으며, 경도는 IP, BP3 및 BP4는 저장 60일에 증가의 폭이 가장 크게 나타났다. 산가는 IP, LP1, LP2, LP3, BP1 및 BP2 경우 저장 40일에 기준치인 2.0 mg KOH/g 이하이었으며, 과산화물가는 IP, LP1, LP2 및 BP1의 경우 기준치인 40.0 meq/kg 미만이 20일째인 것으로 나타났다. 결론적으로 포장재질별로는 LP2와 BP1이 저장성이 우수하였으며 포장방법별로는 대형포장 >봉지포장 >개별포장 순으로 나타났다.

• 미생물학회지
• /
• 제54권2호
• /
• pp.164-166
• /
• 2018

본 연구에서는 2014년 국내에서 식중독 원인식품인 김밥으로부터 분리된 Salmonella Enteritidis의 유전체 분석을 수행하였다. Salmonella Enteritidis MFDS1004839는 한 개의 chromosome (4,679,649 bp)과 plasmid (96,994 bp)로 구성되어있고, 각각의 G + C contents는 52.2%와 49.3%로 확인되었다. chromosome와 plasmid DNA에 예측된 유전자의 총 수는 4,482개의 단백질 코딩유전자와 84개 tRNA, 그리고 22개의 rRNA였다.

• 대한간호학회지
• /
• 제37권6호
• /
• pp.902-913
• /
• 2007

Purpose: The purpose of this study was to identify the effects of a physical activity-behavior modification combined intervention(PABM-intervention) on metabolic risk factors in overweight and obese elementary school children. Methods: Thirty-two participants ($BMI\geq85$ percentile or relative $obesity\geq10$) were allocated to the PABM-intervention group and behavior modification only intervention group. The PABM -intervention was composed of exercise intervention consisting of 50 minutes of physical activity(Hip-hop dance & gym-based exercises) twice a week and the behavior modification intervention consisted of 50 minutes of instruction for modifying lifestyle habits(diet & exercise) once a week. Effectiveness of intervention was based on waist circumference, BP, HDL-cholesterol, TG, and fasting glucose before and after the intervention. Results: The proportion of subjects with 1, 2, 3 or more metabolic risk factors were 28.1, 43.8, and 15.6%, respectively. After the 8-week intervention, waist circumference, systolic BP, diastolic BP, and HDL-cholesterol changed significantly(p<.01) in the PABM group. Conclusion: This provides evidence that a PABM-intervention is effective in changing metabolic risk factors such as waist circumference, systolic BP, diastolic BP, and HDL-cholesterol in overweight and obese elementary school children.

• 한국산학기술학회논문지
• /
• 제17권1호
• /
• pp.526-537
• /
• 2016

연구목적: 본 연구는 대사증후군 구성요인의 군집별 변화양상과 군집별 조합 중에서 가장 많이 분포된 조합들을 파악하여 대사증후군을 예방하고자 시행하였다. 연구방법: 2009년부터 2013년까지 총 2회 검진을 받은 1900명을 대상으로 하였고, 대사증후군의 변화를 살펴보기 위해 정상군과 대사증후군으로 진단된 군 두군으로 나누어 코호트연구를 시행하였다. 연구결과: 대사증후군 구성요인의 조합 상태에 따라 대사증후군 진단율에 영향을 미치는 순서는 2개의 조합에서는 TG+HDL, TG+FBS순이고, 3개의 조합은 WC+TG+HDL, TG+BP+FBS순이었고, 4개의 조합은 WC+TG+HDL+BP, WC+TG+HDL+FBS의 순이었다. 결론: 대사증후군을 예방하기 위해서는 대사증후군 진단율에 영향을 주는 조합을 고려하여 대사증후군 고 위험군을 찾아내어 관리하는 보건프로그램이 필요할 것이다.

• Parasites, Hosts and Diseases
• /
• 제51권3호
• /
• pp.353-355
• /
• 2013

There are approximately 20 known species of the genus Cryptosporidium, and among these, 8 infect immunocompetent or immunocompromised humans. C. hominis and C. parvum most commonly infect humans. Differentiating between them is important for evaluating potential sources of infection. We report here the development of a simple and accurate real-time PCR-based restriction fragment length polymorphism (RFLP) method to distinguish between C. parvum and C. hominis. Using the CP2 gene as the target, we found that both Cryptosporidium species yielded 224 bp products. In the subsequent RFLP method using TaqI, 2 bands (99 and 125 bp) specific to C. hominis were detected. Using this method, we detected C. hominis infection in 1 of 21 patients with diarrhea, suggesting that this method could facilitate the detection of C. hominis infections.

• 한국육종학회지
• /
• 제42권2호
• /
• pp.139-143
• /
• 2010

Late blight caused by Phytophthora infestans is historically a serious epidemic disease in potato and tomato cultivations. Accession L3708 (Solanum pimpinellifolium), a new source for late blight resistance was identified in AVRDC, and carries the resistance gene, Ph-3, incompatible to P. infestans race 3. The AFLP markers linked to Ph-3 were previously developed from the L3708 accession (Chunwongse et al. 2002). To facilitate tomato breeding with the Ph-3 gene, an attempt was made to convert AFLP markers to sequence-characterized amplified region (SCAR) markers. Among 6 AFLP markers, only one AFLP marker, L87, was successfully converted to SCAR marker. The resistance-specific 230 bp AFLP fragment was cloned and sequenced, and the PCR primer amplifying a 123 bp fragment was designed. This SCAR marker could discriminate resistant and susceptible individuals with high stringency. The developed SCAR marker could be used for the marker assisted-selection in tomato breeding programs.

• 한국작물학회지
• /
• 제52권1호
• /
• pp.81-88
• /
• 2007

본 연구는 농진청 종자은행에 보존된 한국, 중국 및 일본 재래종 콩과 한국 야생콩의 SSR profile 작성과 그들의 유전적 구조 해석을 위해 9개의 SSR 마커에 의해 분석되었다. 1. DNA profiling은 유전자좌별로 2,855(Satt458)점$\sim$4,368(Satt197)점이 분석되어 35,655건이 데이터베이스화되었다. 2. 총 대립인자수는 267개였고 유전자좌당 평균 29.6개의 높은 다형성을 나타냈다. 유전자좌별 대립인자 수는 21개(Satt532 및 Satt141)부터 58개(Sat_074)까지 나타났다. 자원내력별 대립인자수는 한국 야생콩에서 196개로 가장 많은 것으로 나타난 반면, 일본 재래종 콩에서는 가장 적은 115개로 나타났다. 3. 집단에 따른 유전자좌별 대립인자의 범위로 한국 재래종 콩이 가장 많은 5개의 유전자좌(Sat_074, Satt141, Satt286, Satt545, Satt458)에서 다음으로는 한국 야생콩이 4개의 유전자좌(Satt187, Satt532, Satt245, Satt197)에서 가장 넓은 것으로 나타났다. 그러나 대립 인자수면에서는 한국 재래종 콩이 5개의 유전자좌(Sat_074, Satt141, Satt197, Satt545, Satt458)에서 가장 많은 대립인자수를 나타냈고, 한국 야생콩은 나머지 4개의 유전자좌(Satt187, Satt532, Satt245, Satt286)에서 가장 많은 대립인자수를 나타냈다. 4. 대립인자 분포에 있어 전체적으로 한국 야생콩 집단은 재래종 집단들에 비해 고른 분포를 나타냈고 대립인자의 크기가 큰쪽(high ladder)에서보다 작은쪽(low ladder)에서 높은 분포를 나타냈다. 5. 재래종 집단들 간에 대립인자 분포를 살펴보면, 한국 집단은 Satt286(202 bp, 232 bp)에서, 중국집단은 Satt197(171 bp)와 Satt458(173 bp)에서 그리고 일본집단은 Sat_074(244 bp)와 Satt458(170 bp)에서 매우 높은 것으로 나타났다.