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Uterotrophic and Hershberger Assay for Butyl ρ-Hydroxybenzoic Acid  

Ahn, Ji-Yun (Food Function Research Civision, Korea Food Research Institute)
Kim, Sung-Ran (Food Function Research Civision, Korea Food Research Institute)
Ha, Tae-Youl (Food Function Research Civision, Korea Food Research Institute)
Kim, Sung-Su (Food Function Research Civision, Korea Food Research Institute)
Han, Chan-Kyu (Food Function Research Civision, Korea Food Research Institute)
Publication Information
Toxicological Research / v.21, no.2, 2005 , pp. 175-178 More about this Journal
Abstract
Butyl $\rho$-hydroxybenzoic acid (butyl paraben, BP) is a homologous series of parabens and is widely used as a preservative in cosmetic and pharmaceutical products. The purpose of this study was to investigate the estrogenic/antiandrogenic activities of BP in animals. For that, we performed an uterotrophic assay and a Hershberger assay in rats. In uterotrophic assay, BP was administered subcutaneously to immature female SD rats (18 days old) for 3 consecutive days. The wet and dry uterus weights were significantly increased in the groups treated with BP in dose­dependent manner. In case of Hershberger assay, BP significantly reduced the weight of seminal vesicle of castrated rats. And other accessory organ/glands - prostate, Cowper's glands, bulbocavernosus muscle and glans penis were also slightly decreased. The results of this study suggested that BP showed estrogenic and anti-androgenic activities in vivo.
Keywords
Uterotrophic assay; Hershberger assay;
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