• Herbal Formula Science
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• v.24 no.1
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• pp.17-30
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• 2016

Objectives Rehmanniae Radix Preparata was prepared in the traditional Rehmanniae Radix Preparata preparation method set forth in the 『Treasured Mirror of Eastern Medicine(Donguibogam)』 with a view to measuring the contents of 5-Hydroxymethyl-2-furaldehyde(5-HMF) at individual stages of steaming and sundrying and identifying new chemical components.Methods Based on the traditional Rehmanniae Radix Preparata preparation method set forth in the 『Treasured Mirror of Eastern Medicine』, Rehmanniae Radix Preparata steamed and sundried once through nine times was prepared. Thereafter, 5-HMF contents were analyzed and new chemical components were identified in the Rehmanniae Radix Preparata using Waters HPLC e2695, 2640 detectors, a Waters Acquity UPLC system, and a Micromass Q-TOF Premier mass spectrometer.Results The Rehmanniae Radix Preparata preparation method set forth in the 『Treasured Mirror of Eastern Medicine』 is a unique preparation method in Republic of Korea different from that in China. In the first stage of the method, fresh Rehmanniae Radix Crudus was divided into high quality, medium quality, and low quality ones named Rehmanniae Radix Crudus (Caelum)(天黃), Rehmanniae Radix Crudus (Homo)(人黃), and Rehmanniae Radix Crudus (Terra)(地黃) respectively to use Rehmanniae Radix Crudus (Caelum) and Rehmanniae Radix Crudus (Homo) for preparation of juice while using Rehmanniae Radix Crudus (Terra) to make Rehmanniae Radix Preparata. In the second stage, Rehmanniae Radix Crudus (Caelum) and Rehmanniae Radix Crudus (Terra) were made into juice and Rehmanniae Radix Crudus (Terra) was soaked in the juice. In the third stage, among auxiliary materials, rice wine named Purum Vinum Oryzae(淸酒) brewed from sticky rice was sprinkled on Rehmanniae Radix Crudus (Terra) to the extent that Rehmanniae Radix Crudus (Terra) became wet. In the fourth stage, Rehmanniae Radix Preparata steamed in earthenware steamer was dried under natural sunlight. The contents of 5-HMF in Rehmanniae Radix Preparata steamed and sundried once through nine times were shown to be below 0.1% in all cases. Pomolic acid was identified as a new chemical component.Conclusions In conclusion, the Rehmanniae Radix Preparata preparation method set forth in the 『Treasured Mirror of Eastern Medicine』 is thought to be a unique preparation method in South Korea in which Rehmanniae Radix Preparata is completed through the first stage in which fresh Rehmanniae Radix Crudus collected from fields is divided into high, medium, and low quality ones and fresh Rehmanniae Radix Crudus juice is made, the second stage in which the high quality fresh Rehmanniae Radix Crudus is soaked in the fresh Rehmanniae Radix Crudus juice, the third stage in which the fresh Rehmanniae Radix Crudus is steamed, and the fourth stage in which the steamed Rehmanniae Radix Crudus is dried.

• The Journal of Korean Medicine
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• v.20 no.1 s.37
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• pp.84-90
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• 1999

In order to analyze the changes in the contents of Rehmanniae Radix during processing, the constituents of Rehmanniae Radix Preparata, such as catalpol, 5-HMF and the carbohydrates, were analyzed, The results were: 1. The catalpoI contents of Rehmanniae Radix Preparata remarkably decreased during the 1-4th steps in the processing, and the concentrations of 5-9th Rehmanniae Radix Preparatas were very low. 2. The contents of 5-HMF increased gradually with each additional processing. and the increasing rate in oven-dried Rehmanniae Radix Preparata was greater by about 2-folds than that of sun-dried. 3. Rehmanniae Radix contained a lot of sugars. such as rhamnose, raffinose and stachyose. In the course of the process for sun-dried or oven-dried Rehmanniae Radix Preparata., rhamnose disappeared during the 1-2nd step, but raffinose and stachyose stayed the same. 4. The level of fructose, developed at the first processing step, stayed the same during each additional processing for sun-dried Rehmanniae Radix Preparata. But, the level gradually decreased after the 6th processing step for oven-dried Rehmanniae Radix Preparata.

• Journal of Haehwa Medicine
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• v.9 no.2
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• pp.173-182
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• 2001

1. $\ll$Graphical Materia Medica$\gg$ is first book that writes about Rehmanniae Radix Preparat. $\ll$Shen nong's herbal$\gg$ is only written about Rehmanniae Radix. 2. After the $\ll$Decoction Materia Medica$\gg$, orintal dortors use Rehmanniae Radix's effect is removing heat from the blood and Rehmanniae Radix's effect is enriching the blood. 3. Rehmanniae Radix Preparat is kinds of durgs for enriching the blood, because Rehmanniae Radix Preparat's effect do nourishing yin and supplementing blood. Therefore, Rehmanniae Radix Preparat is uesd simple recipe or Rehmanniae Radix Preparat is used with another herbs that shows so good effect of hematogenous functions at laboratory work. 4. Bone marrow is doing hematogenesis. That has relation to Rehmanniae Radix Preparat's effect is replenishing bone marrow, it's channel tropisms are liver kidney channels. 5. Rehmanniae Radix Preparat's guide component is 5-HMF, but effective component has relation with hematogenesis is mannitol of saccharides. And we need more reserch about Rehmanniae Radix Preparat.

• Journal of Physiology & Pathology in Korean Medicine
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• v.26 no.3
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• pp.306-313
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• 2012

Herbal medicines are medicinal products containing a single or a mixture of two or more different herbal substances or herbal preparations as active principles. Recently, much attention has been paid to developing various kinds of fermented herbal extracts, a new type of traditional herbal medicine in the field of Korean traditional medicine. The fermentation of medicinal herbs is intended to exert a favorable influence on bioestability, bioavaliablilty and pharmacological activity of herbal extract in the gastrointestinal tract as well as intensifying the nutritional and pharmacological aspects of the medicinal herbs. The purpose of this study was to investigate biological activities of fermented Rehmanniae Radix by lactic acid bacteria at $30^{\circ}C$ for 3 days in comparison with those for Rehmanniae Radix The fermented Rehmanniae Radix exhibited different chemical profile to Rehmanniae Radix generated with HPLC, indicating production of new ingredients during fermentation. Rehmanniae Radix served as good nutritional sources for the growth of lactic acid bacteria showing increased number of bacteria during fermentation. Toxic effect of the fermented Rehmanniae Radix to cells were not seen judged by the MTT assay. The fermented Rehmanniae Radix exhibited better antioxidant effect than non-fermented Rehmanniae Radix analyzed by a SOD-likely assay. Both hypoglycemic and hypotensive effects of the fermented Rehmanniae Radix were also detected and better than those for Rehmanniae Radix in showing dose-dependent inhibitory effects on alpha-glucosidase and ACE, respectively. In conclusion, fermented Rehmanniae Radix appears to have more biological activities than non-feremented Rehmanniae Radix showing not only antioxidant effect but also cardiovascular protection.

• Analytical Science and Technology
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• v.6 no.4
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• pp.401-404
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• 1993

Rehmanniae Radix Preparata is manufactured with Rehmanniae Radix accoding to KP V. For quality control of Rehmanniae Radix Preparata, its standard component is required. The methanol extracts of Rehmanniae Radix crudus, Rehmanniae Radix, Rehmanniae Radix preparata were divided into the three groups of ether, butanol and aqueous fraction by liquid-liquid separation. In the comparative TLC of ether fraction, the characteristic component of Rehmanniae Radix preparata was found. The ether fraction was evaporated and separated on the silica gel column with chloroform-methanol and further separated on the preparative silica gel TLC with chloroform-methanol-water. The component was illucidated as 5-(hydroxymethyl)-2-furancarboxaldehyde(5-HMF). 5-HMF was not found in Rehmanniae Radix crudus and found in Rehmanniae radix in much less Quantities than Rehmanniae Radix Preparata.

• Journal of Physiology & Pathology in Korean Medicine
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• v.25 no.5
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• pp.823-829
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• 2011

This study was performed to obtain the good processing in the Rehmanniae Radix Preparata. In this study, we investigated the comparative analysis for steamed and hot-air dried Rehmanniae Radix Preparata. The results were obtained as follows : Through loss on drying analysis with each additional processing of Rehmanniae Radix preparata, there was gradual increase. Through ash analysis with each additional processing of Rehmanniae Radix preparata, there was slight increase. Rehmanniae Radix preparata had several inorganic components such as K, P, Mg, Ca, Cl, Al. P and Mg were increased gradually with each additional processing of Rehmanniae Radix Preparata. But Ca was decreased with each additional processing. We identitied 24 kinds of volatile components from extracts of various processed Rehmanniae Radix Preparata through componential analysis using GC/MS. From GC/MS analysis, we found out the content of 5-HMF Rehmanniae Radix Preparata steamed over 7 times was suitable. 5-HMF's content of 9 times steamed Rehmanniae Radix Preparata gained from GC/MS analysis is 70-fold higher than gained from HPLC. The more it is repeating steamed and hot-air dried, the more toxic volatile compound of Rehmanniae Radix Preparata is removed. Taken together, these results suggested that Rehmanniae Radix Preparata had component (especially 5-HMF) changes according to different processed steaming and hot-air drying.

• Herbal Formula Science
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• v.18 no.1
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• pp.133-144
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• 2010

Object : The effects of Rehmanniae Radix(RR) on HPA Axis system and Catecholaminergic system was investigated. Methods : we performed the Forced Swimming Test(FST). Also the expression of Corticotropin -Releasing Factor(CRF), c-Fos and Tyrosine Hydorxylase(TH) was measured by immunohistochemical method at Paraventricular Nucleus(PVN), Locus Coeruleus(LC) and Ventral Tegmental Area(VTA). Results : 1. The duration of immobility in the Forced Swimming Test was significantly decreased in the Rehmanniae Radix 400 mg/kg groups in comparison with the control group(p<0.05). 2. Comparing to the control group, CRF expression was significantly decreased in the Rehmanniae Radix 400 mg/kg treated group in comparison with the control group(p<0.01). 3. c-Fos expression was significantly decreased at PVN in the Rehmanniae Radix 100 mg/kg treated group in comparison with the control group(p<0.01). 4. TH expression was significantly increased at LC in the Rehmanniae Radix 100 mg/kg and 400 mg/kg treated group in comparison with the control group(p<0.001). 5. TH expression was significantly decreased at VTA in the Rehmanniae Radix 100 mg/kg and 400 mg/kg treated group in comparison with the control group(p<0.001). Conclusion : According to the results, it can be considered that Rehmanniae Radix has antidepressant effect by showing the reduction of immobility in FST through the decreased expression of CRF, c-Fos in PVN and TH in VTA.

• Korean Journal of Pharmacognosy
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• v.36 no.2 s.141
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• pp.75-80
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• 2005

This study is concerning to identify the hygienic problems occurring in processing, storage, and distribution of Rehmanniae radix crude, and to investigate the possibility of application of safe and hygienic ${\gamma}-irradiation$ techniques. The results are as follows. To compare the contents of catapol, index compound of Rehmanniae Radix crude, between before and after ${\gamma}-irradiated$,0,2.5,5,7.5,10,12.5,15,20, and 30 kGy of ${\gamma}-irradiation$ was applied to standard catapol and Rehmanniae radix crude. The contents of catapol in standard material and Rehmanniae radix crude were decreased with the increase of ${\gamma}-irradiation$ level. However, active components of Rehmanniae Radix crude were not changed with ${\gamma}-irradiation$. These results indicate that active components of Rehmanniae Radix crude were preserved after ${\gamma}-irradiation$ and those of Rehmanniae Radix crude did not showed significant change after irradiation of ${\gamma}-ray$ up to 20 kGy.

• Journal of Pharmacopuncture
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• v.20 no.2
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• pp.81-88
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• 2017

Objectives: This study was a review of the literature published on the efficacy of Rehmanniae Radix to obtain information to provide both a foundation for its clinical use and directions for future studies. Methods: This study selected 30 publications registered to databases or published in Korea by March 13, 2017, by searching electronic journal databases, i.e., NDSL, OASIS, RISS, and KISS, in order to review the efficacy of Rehmanniae Radix. The search was conducted using the keywords '生地黃', '鮮地黃', 'Rehmannia Glutinosa', and 'Rehmanniae Radix', and the search results were compiled and analyzed. Results: The selected publications included antioxidant effects (12 publications), data involving blood glucose reduction (4 publications), effects on the autonomic nervous system (3 publications), and effects on the human body (11 publications). Conclusion:The results of this literature review indicate that Rehmanniae Radix is effective in treating patients with various inflammatory and metabolic diseases, such as high blood pressure and diabetes. Based on these results, we believe that Rehmanniae Radix can be used effectively to treat patients with such diseases.

• Journal of Physiology & Pathology in Korean Medicine
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• v.36 no.1
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• pp.11-17
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• 2022

In this study, we investigated the effects of Ginseng Radix (G), Rehmanniae Radix (R), and Fermented red-ginseng extracts (FRG) on cognitive function in scopolamine-induced memory-impaired mice. We measured the effects of G, R, and FRG on the improvement of memory and cognition via behavior analysis. In addition, we measured the acetylcholinesterase (AChE) activity in the hippocampus of each group of mice. The expression of β-amyloid, Tau, and BDNF in the brain tissues were observed through immunohistochemical staining. Ginseng Radix (G) and Fermented red-ginseng (FRG) have effectively improved cognitive function in the water maze test. Ginseng Radix (G), Rehmanniae Radix (R), and Fermented red-ginseng (FRG) have improved the willingness of mice to explore the new environment, as confirmed by Y maze test. In addition, immunohistochemical staining revealed that Ginseng Radix (G) decreased the expression of β-amyloid and Tau in the hippocampus. In addition, fermented red-ginseng (FRG) increased the expression of BDNF. Ginseng Radix (G), Rehmanniae Radix (R), and Fermented red-ginseng (FRG) have decreased the concentration of acetylcholinesterase in the hippocampus as compared with the control group of mice. In conclusion, Ginseng Radix (G), Rehmanniae Radix (R), and Fermented red-ginseng (FRG) are considered to have the potential for development as candidate drugs to control the progression of Alzheimer's disease (AD).