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http://dx.doi.org/10.15188/kjopp.2022.02.36.1.11

Comparative Study of Ginseng Radix, Rehmanniae Radix and Fermented Red-ginseng Extracts Effects on Memory Improvement in Scopolamine-induced Memory Impaired Mice  

Kim, Ji Hyun (Department of Food & Biotechnology, College of Food Science, Woosuk University)
Kim, Jong Uk (Department of Acupuncture & Moxibustion, College of Korean Medicine, Woosuk University)
Lee, Chang Hyun (Department of Anatomy, College of Korean Medicine, Woosuk University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.36, no.1, 2022 , pp. 11-17 More about this Journal
Abstract
In this study, we investigated the effects of Ginseng Radix (G), Rehmanniae Radix (R), and Fermented red-ginseng extracts (FRG) on cognitive function in scopolamine-induced memory-impaired mice. We measured the effects of G, R, and FRG on the improvement of memory and cognition via behavior analysis. In addition, we measured the acetylcholinesterase (AChE) activity in the hippocampus of each group of mice. The expression of β-amyloid, Tau, and BDNF in the brain tissues were observed through immunohistochemical staining. Ginseng Radix (G) and Fermented red-ginseng (FRG) have effectively improved cognitive function in the water maze test. Ginseng Radix (G), Rehmanniae Radix (R), and Fermented red-ginseng (FRG) have improved the willingness of mice to explore the new environment, as confirmed by Y maze test. In addition, immunohistochemical staining revealed that Ginseng Radix (G) decreased the expression of β-amyloid and Tau in the hippocampus. In addition, fermented red-ginseng (FRG) increased the expression of BDNF. Ginseng Radix (G), Rehmanniae Radix (R), and Fermented red-ginseng (FRG) have decreased the concentration of acetylcholinesterase in the hippocampus as compared with the control group of mice. In conclusion, Ginseng Radix (G), Rehmanniae Radix (R), and Fermented red-ginseng (FRG) are considered to have the potential for development as candidate drugs to control the progression of Alzheimer's disease (AD).
Keywords
Cognitive impairment; Ginseng Radix; Rehmanniae Radix; Fermented red-ginseng; Behavioral analysis; AChE activity;
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Times Cited By KSCI : 6  (Citation Analysis)
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1 Kim SH. Comparative Study of Ginseng Radix and Rehmanniae Radix extracts on Alzheimer's mouse model(APPswePSEN1dE9) by Behavior and Molecular Factors. Doctoral Thesis. Woosuk University, 2017.
2 Kee JY, Hong SH, Park JH. Effect of Albizziae cortex water extract on cognition and memory impairments. J Physiol & Pathol Korean Med. 2017;31(5):270-6.   DOI
3 Hardy JA, Higgins GA. Alzheimer's disease: the amyloid cascade hypothesis. Science, 1992;256(5054):184-6.   DOI
4 Wang LC, Wang B, Ng SY, Lee TF. Effects of ginseng saponins on β-amyloid-induced amnesia in rats. J Ethnopharmacol. 2006;103(1):103-8.   DOI
5 Sarter M, Bodewitz G, Stephens DN. Attenuation of scopolamine-induced impairment of spontaneous alternation behaviour by antagonist but not inverse agonist and agonist β-carbolines. Psychopharmacology. 1988;94(4):491-5.   DOI
6 Oide T, Kinoshita T, Arima K. Regression stage senile plaques in the natural course of Alzheimer's disease. Neuropathol Appl Neurobiol. 2006;32(5):539-56.   DOI
7 Kim SY. Past and future of drug treatments for Alzheimer's disease. J Korean Neuropsychiatr Assoc. 2018;57(1):30-42.   DOI
8 Kang SJ, Woo JH, Kim AJ. The effects of Korean ginseng on memory loss in a rat models. J Korean Soc Food Sci Nutr. 2013;42(8):1190-96.   DOI
9 Sohn KH, Kim JS. Anti-dementia effects of Cornus officinalis S. et Z. extract on the scopolamine induced dementia in mouse. Kor J Pharmacogn. 2017;48(4):304-13.
10 Kuhl DE, Koeppe RA, Minoshima S, Snyder SE, Ficaro EP, Foster NL, Kilbourn MR. In vivo mapping of cerebral acetylcholinesterase activity in aging and Alzheimer's disease. Neurology. 1999;52(4):691-9.   DOI
11 Lee JH, Kim HJ, Lee CH, Park SH, Jung CJ, Beik GY, Jung JW. Comparative study on the effects of Korean and Chinese crataegus pinnatifida on scopolamine-induced memory impairment in mice. J Physiol & Pathol Korean Med. 2018;32(6):375-83.   DOI
12 Lee MR, Sun BS, Gu LJ, Wang CY, Mo EK, Yang SA, Sung CK. Effects of white ginseng and red ginseng extract on learning performance and acetylcholinesterase activity inhibition. J Ginseng Res. 2008;32(4):341-6.   DOI
13 Yuan QL, Yang CX, Xu P, Gao XQ, Deng L, Chen P, Chen QY. Neuroprotective effects of ginsenoside Rb1 on transient cerebral ischemia in rats. Brain Res. 2007;1167:1-12.   DOI
14 Yoo SK, Kim JM, Park SK, Kang JY, Han HJ, Park HW, Heo HJ. Protective effect of Gabjubaekmok (Diospyros kaki) extract against amyloid beta (Aβ)-induced cognitive impairment in a mouse model. Korean J Food Sci Technol. 2019;51(4):379-92.   DOI
15 Lee SG, Chai YG, Jung KH, Kim JH. Gene expression profiling of SH -SY5Y cells in neuroprotective effect of total ginsenosides on H202 induced neurotoxicity. J Orient Neuropsychiatry. 2007;18(1):95-110.
16 Bae HK, Seo BI. Inhibitory activities of Rehmanniae radix 30% ethanol extract on acute gastritis and peptic ulcers. Kor J Herbol 2019;34(2):1-14.   DOI
17 Ye L, Zhou CQ, Zhou W, Zhou P, Chen DF, Liu XH, Shi XL, Feng MQ. Biotransformation of ginsenoside Rb1 to ginsenoside Rd by highly substrate-tolerant Paecilomyces bainier 229-7, Bioresource Technology 2010;101:7871-6.
18 Cerejeira J, Lagarto L, Mukaetova-Ladinska E. Behavioral and psychological symptoms of dementia. Front Neurol. 2012;3:73.   DOI
19 Ha DC, Ryu GH. Chemical components of red, white and extruded root ginseng. J Korean Soc Food Sci Nutr. 2005;34(2):247-54.   DOI
20 Park HJ, Son CG. Systematic analysis of Ginseng-focused research worldwide. J Korean Oriental Med. 2008;29(1):60-6.
21 Cheon GY. Screening Antioxidants of Rehmannia glutinosa. Master,s Thesis, Mokpo university, 2018.
22 Hsu SM, Raine L, Fanger HX. Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem. 1981;29(4):577-80.   DOI
23 Cummings J, Lee G, Ritter A, Sabbagh M, Zhong K. Alzheimer,s disease drug development pipeline: 2019. Alzheimer,s & Dementia: Translational Research & Clinical Interventions. 2019;5:272-93.   DOI
24 Yoon JC, Lee SR, Jung IC. The Effects of HyungBangSaBaek-San (JingFangXieBaiSan) on the Alzheimer's disease model induced by βA. J Orient Neuropsychiatry. 2010;21(2):171-89.
25 Jeong HC, Hong HD, Kim YC, Rho J, Kim KT, Cho CW. The research trend of ginseng processing technology and the status of ginseng industry. Food Science and Industry. 2012;45(4): 59-67.   DOI
26 Ha DC, Ryu GH. Chemical components of red, white and extruded root ginseng. J Korean Soc Food Sci Nutr. 2005;34(2):247-54.   DOI
27 Kwak YS, Park JD, Yang JW. Present and its prospect of red ginseng efficacy research. J Korean Soc Food Sci Nutr. 2003;8(2):30-7.
28 Wang SB, Ahn EM, Jung JW. The fruits of Crataegus pinnatifida Bunge ameliorates learning and memory impairments induced by scopolamine. Kor J Herbology. 2009;24(4):165-71.
29 Kim HR, Lee CH, Jung MY, Kim JS, Kim HJ, Jeon HS, Lee SH, Kim JH, Shin MJ, Ma SY, Kwon J, Oh CH. Anti-obesity effects of cultivated ginseng, -wild simulated ginseng and-red ginseng extracts. Herbal Formula. Science. 2019;27(4)269-84.
30 Park KH, Kim YS, Jeong JH. Inhibitory effects of ginseng extracts on histamine-release from rat's mast cell. Korean J. Plant Res. 2011;24(1):98-104.   DOI
31 Kuhl DE, Koeppe RA, Minoshima S, Snyder SE, Ficaro EP, Foster NL, Kilbourn MR. In vivo mapping of cerebral acetylcholinesterase activity in aging and Alzheimer's disease. Neurology. 1999;52(4):691-9.   DOI
32 Kim YJ. Isolation and Characterization of AChE inhibitory compounds from natural products and their action mechanisms. Seoul National University, 2004.
33 Kim JH, He MT, Kim MJ, Park CH, Lee JY, Shin YS, Cho EJ. Protective effects of combination of carthamus tinctorius L. Seed and Taraxacum coreanum on scopolamine-induced memory impairment in mice. Korean J. Medicinal Crop Sci, 2020;28(2):85-94.   DOI