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The Plant Cellular Systems for Plant Virus Movement

  • Hong, Jin-Sung;Ju, Ho-Jong
    • The Plant Pathology Journal
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    • v.33 no.3
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    • pp.213-228
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    • 2017
  • Plasmodesmata (PDs) are specialized intercellular channels that facilitate the exchange of various molecules, including sugars, ribonucleoprotein complexes, transcription factors, and mRNA. Their diameters, estimated to be 2.5 nm in the neck region, are too small to transfer viruses or viral genomes. Tobacco mosaic virus and Potexviruses are the most extensively studied viruses. In viruses, the movement protein (MP) is responsible for the PD gating that allows the intercellular movement of viral genomes. Various host factors interact with MP to regulate complicated mechanisms related to PD gating. Virus replication and assembly occur in viral replication complex (VRC) with membrane association, especially in the endoplasmic reticulum. VRC have a highly organized structure and are highly regulated by interactions among the various host factors, proteins encoded by the viral genome, and the viral genome. Virus trafficking requires host machineries, such as the cytoskeleton and the secretory systems. MP facilitates the virus replication and movement process. Despite the current level of understanding of virus movement, there are still many unknown and complex interactions between virus replication and virus movement. While numerous studies have been conducted to understand plant viruses with regards to cell-to-cell movement and replication, there are still many knowledge gaps. To study these interactions, adequate research tools must be used such as molecular, and biochemical techniques. Without such tools, virologists will not be able to gain an accurate or detailed understanding of the virus infection process.

Antioxidative and Physical Characteristics of Rice Germ added Sweet Pumpkin Rice Snack for Infants (영아용 쌀눈 첨가 단호박 쌀 스낵의 항산화적 물리적 특성)

  • Wu, Xiubao;Kim, Eunkyung;Choi, Kunyoung;Kim, Haeyoung
    • Journal of the Korean Society of Food Culture
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    • v.33 no.1
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    • pp.62-69
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    • 2018
  • The purpose of this study was to investigate the antioxidative and physical properties of finger food type rice snacks and pumpkin rice snacks for infants aged 6 to 12 months old. The addition of rice germ did not affect density or porosity, and there was no significant difference when compared with commercial products. The addition of rice germ to the rice snack group did not reduce the longitudinal expansion quality of the rice snack. The WAI of RW and SW values were 9.83 and 8.93, respectively, which were significantly higher than those of other samples (p<0.05). The results of this study show that the addition of rice germ to rice snack resulted in reduced density and porosity of rice snacks and maintenance of a high water absorption index, which are more desirable characteristics for products. The products with rice germ showed significantly higher antioxidative activities among all samples (p<0.05).

Antimicrobial Cyclic Peptides for Plant Disease Control

  • Lee, Dong Wan;Kim, Beom Seok
    • The Plant Pathology Journal
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    • v.31 no.1
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    • pp.1-11
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    • 2015
  • Antimicrobial cyclic peptides derived from microbes bind stably with target sites, have a tolerance to hydrolysis by proteases, and a favorable degradability under field conditions, which make them an attractive proposition for use as agricultural fungicides. Antimicrobial cyclic peptides are classified according to the types of bonds within the ring structure; homodetic, heterodetic, and complex cyclic peptides, which in turn reflect diverse physicochemical features. Most antimicrobial cyclic peptides affect the integrity of the cell envelope. This is achieved through direct interaction with the cell membrane or disturbance of the cell wall and membrane component biosynthesis such as chitin, glucan, and sphingolipid. These are specific and selective targets providing reliable activity and safety for non-target organisms. Synthetic cyclic peptides produced through combinatorial chemistry offer an alternative approach to develop antimicrobials for agricultural uses. Those synthesized so far have been studied for antibacterial activity, however, the recent advancements in powerful technologies now promise to provide novel antimicrobial cyclic peptides that are yet to be discovered from natural resources.

The sound insulation performance of eco-friendly loess brick wall (친환경 황토벽체의 차음성능 평가에 관한 연구)

  • Lee, Tai-Gang;Kim, Yul;Song, Kook-Gon;Kim, Sun-Woo
    • KIEAE Journal
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    • v.9 no.6
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    • pp.13-18
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    • 2009
  • Korean traditional houses have been developed in harmony with natural environment and comfortable indoor condition by using the natural resources including building layout, space composition and materials. Originally Korea traditional architectures have used wood lintel constructions and loess walls through the many years. Theses loess have many strength such as highly heat capacity, controling of humidity, a deodorant than any other materials. Nowaday it is recommended to use exterior and interior walls in loess wall to meet the eco-friendly materials to improve our residental environmental. Thus this study aims to research the sound insulation performance of traditional loess brick wall varied with thickness, thermal insulation materials and cavity wall. The sound insulation performance of these loess walls are compared with other masonry wall's and sound insulation performance of th walls were tested in anechoic laboratory to measure the sound transmission loss of these walls. The loess brick wall with 75mm thickness of cavity is shown the sound insulation performance with Rw 57 which is nearly same performances of 1B brick wall and cement 8' block wall, The improving effect of insulation materials is shown in the high frequency bandwidth. Especially, there is improving as much as 11 dB using the extruded poly stylene form(75mm) and poly ethylene film(0.7mm).

The Effect of Lycii fructus beer intake on serum lipid profiles and antioxidant activity in rats (구기자 맥주의 섭취가 흰쥐의 혈청 지질패턴 및 항산화효소 활성에 미치는 영향)

  • Chung, Hae-Kyung;Choi, Chang-Suk;Yang, Eun-Ju;Kang, Myung-Hwa
    • Journal of the Korean Society of Food Culture
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    • v.19 no.1
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    • pp.52-60
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    • 2004
  • This study was performed to investigate the effect of Lycii fructus beer on serum lipid profiles and antioxidant activity in rat Sprague-Dawley (SD) rats weighting about 190g were divided into the following 5 groups ; distillate water (Control), 5% ethanol in distillate water (Ethanol), commercial beer (CB), Lycii fructus beer (LFB) and 5% alcohol red wine diluted with distillate water (RW). Body weight, total food intake, FER and percent organ (liver, kidney) weight per body weight were not significantly changed by Lycii fructus beer drinking. After 6 weeks, serum total cholesterol, triglyceride and HDL cholesterol level were not significantly different. But, Lycii fructus beer intake tended to decrease serum triglyceride level and atherogenic index. Also, GOT and GPT levels were expressed lower than Ethanol group. There was not significantly different in hepatic glutatiione (GSH) content and glutathione-S-transferase (GST) activities among 5 groups. Lipid peroxidation in the hepatic was decreased by Lycii fructus beer intake. The results demonstrated that Lycii fructus beer was potential and effective antioxidant that can protect the decrease associated with alcohol.

A 3-Phase Spindle Motor Stable Control by Using Common Terminal (Common 단자를 이용한 3상 Spindle Motor 안정제어)

  • SeoMoon, Kite
    • Proceedings of the KIEE Conference
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    • 2007.07a
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    • pp.294-295
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    • 2007
  • 80년대 초에 개발된 CD(Compact Disc) Disc는 지난 27여 년간 급속한 발전을 하였다. 95년에 DVD가 발표되면서 영화 Title제작이 되어 우리는 안방에서 고화질의 DVD를 감상할 수 있었다. CD-R(Recordable) 로부터 시작된 기록 기술은 CD-RW를 거쳐 DVD-Recordable, DVD-ReWritable 및 DVD-RAM, Light Scribe등 거쳐 BD(Blu-Ray) 기술에 까지 이르게 되었다. 광 Disc 기술은 3가지 분야로 나눌 수 있다. 안정된 제어를 위한 Servo 기술, Mechanical 기술, PC와communication 할 수 있는 Interface 기술 등으로 나눌 수 있다. Servo 제어 기술은 다음과 같이 4가지 분야로 구성되어 있다. Focus Servo, Tracking Servo, Sled Servo, Spindle Servo. Disc 회전을 제어를 담당하고 있는 Spindle Servo Block에서는 Hall Sensor를 이용하여 Disc의 회전을 Count하여 Feedback 하였다. 그러나 갈수록 심화 되고 있는 가격 경쟁 상황에서 Hall Sensor를 대체하여 회전수를 Conut하는 방법이 필요 하였다. BEMF(역기전력)을 이용한 Zero Crossing 방법이 이를 대체 하였지만, Motor의 전기적인 특성 편차 등에 안정된 control이 안되어서, Motor를 관리하는 방법으로 진행되어 왔다. 이것은 생산 Loss로 이어져서 Hall Sensorless의 장점을 훼손하게 되었다..본 논문에서는 이러한 Sensorless Type Spindle Motor의 문제점에 대해 Common 단자를 이용하여 안정된 제어 방법을 제안 한다. less Type의 문제점인 초기 기동 문제를 Common 단자를 이용하여 안정되게 Spindle motor를 Control 하는 방법에 대해 연구 하고자 한다.

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Occurrence and Epidemics of Bacterial Canker of Kiwifruit in Korea

  • Kim, Gyoung Hee;Jung, Jae Sung;Koh, Young Jin
    • The Plant Pathology Journal
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    • v.33 no.4
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    • pp.351-361
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    • 2017
  • Bacterial canker is the largest limiting factor in the cultivation and production of kiwifruit worldwide. Typical symptoms comprise necrotic spots on leaves, canker and dieback on canes and trunks, twig wilting, and blossom necrosis. Pseudomonas syringae pv. actinidiae (Psa), which is the causal agent of kiwifruit bacterial canker, is divided into four biovars based on multilocus sequence analysis of different genes, additional PCR testing of pathogenic genes (argKtox cluster, cfl, and various effector genes), and biochemical and physiological characterization. Bacterial canker caused by Psa biovar 2 designated Psa2 was detected for the first time on the green-fleshed kiwifruit cultivar Hayward in 1988 and the yellow-fleshed kiwifruit cultivar Hort16A in 2006 in Korea. Psa biovar 3 designated Psa3, responsible for the current global pandemics of kiwifruit bacterial canker, began to appear in Korea in 2011 and caused tremendous economic losses by destroying many vines or orchards of yellow-fleshed kiwifruit cultivars in one or several growing seasons. Bacterial canker epidemics caused by both Psa2 and Psa3 are prevalent in Korea in recent years. In this review, we summarize the symptomatology, etiology, disease cycle, diagnosis, and epidemiology of kiwifruit bacterial canker in Korea.

Bacteriophage Usage for Bacterial Disease Management and Diagnosis in Plants

  • Vu, Nguyen Trung;Oh, Chang-Sik
    • The Plant Pathology Journal
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    • v.36 no.3
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    • pp.204-217
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    • 2020
  • In nature, plants are always under the threat of pests and diseases. Pathogenic bacteria are one of the major pathogen types to cause diseases in diverse plants, resulting in negative effects on plant growth and crop yield. Chemical bactericides and antibiotics have been used as major approaches for controlling bacterial plant diseases in the field or greenhouse. However, the appearance of resistant bacteria to common antibiotics and bactericides as well as their potential negative effects on environment and human health demands bacteriologists to develop alternative control agents. Bacteriophages, the viruses that can infect and kill only target bacteria very specifically, have been demonstrated as potential agents, which may have no negative effects on environment and human health. Many bacteriophages have been isolated against diverse plant-pathogenic bacteria, and many studies have shown to efficiently manage the disease development in both controlled and open conditions such as greenhouse and field. Moreover, the specificity of bacteriophages to certain bacterial species has been applied to develop detection tools for the diagnosis of plant-pathogenic bacteria. In this paper, we summarize the promising results from greenhouse or field experiments with bacteriophages to manage diseases caused by plant-pathogenic bacteria. In addition, we summarize the usage of bacteriophages for the specific detection of plant-pathogenic bacteria.

Natural Frequencies of a Spinning Disk Misaligned with the Axis of Rotation (회전축 정렬불량을 가지는 유연 회전디스크의 고유치 해석)

  • 허진욱;정진태;김원석
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 2001.11b
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    • pp.817-825
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    • 2001
  • The natural frequencies of a flexible spinning disk misaligned with the axis of rotation are studied in an analytic manner. The effects of misalignment on the natural frequency need to be investigated, because the misalignment between the axis of symmetry and the axis of rotation cannot be avoided in the removable disks such as CD-R, CD-RW or DVD disks. Assuming that the in-plane displacements are in steady state and the out-of-plane displacement is in dynamic state, the equations of motion are derived for the misaligned spinning disk. After the exact solutions are obtained for the steady-state in-plane displacements, they are plugged into the equation for the dynamic-state out-of-plane motion. The resultant equation is a linear equation for the out-of-plane displacement, which is discretized by the Galerkin method. Based on the discretized equations, the effects of the misalignment are analyzed on the vibration characteristics of the spinning disk, i.e., the natural frequencies and the critical speed

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The Crystal and Molecular Structure of 1-(3-Chloro-2-hydroxypropyl)-2-methyl-5-nitroimidazole (Ornidazole), $C_7H_{10}CIN_3O_3$

  • 신현소;송현;김의성;정광보
    • Bulletin of the Korean Chemical Society
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    • v.16 no.10
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    • pp.912-915
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    • 1995
  • Ornidazole, C7H10ClN3O3, crystallizes in the triclinic, space group P1^, with a=13.605(2), b=14.054(1), c=8.913(5) Å, α=71.59(2), β=78.73(2), γ=64.86(1)°, μ=3.26 cm-1, Dc=1.499 g/cm3, Dm=1.497g/cm3, F(000)=684, and z=6. Intensities for 2693 unique reflections were measured on a CAD4 diffractometer with graphite-monochromated Mo-Kα radiation. The structure was solved by direct method and refined by block-diagonal least squares to a final R of 0.081 (Rw=0.047) for 1952 reflections with Fo>3σ (Fo). The asymmetric unit contains three independent molecules of the title compound. The bond lengths and bond angles are comparable with the values found in the other nitro-substituted compounds. The nitro groups are rotated (6.9°, 6.6°, 2.6° for the three independent molecule, respectively) about the C-N axes from the imidazole planes. The crystal structures are linked by two intermolecular hydrogen bonds of O-H---N type and one intermolecular hydrogen bond of O-H---O type.