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Roles for α1-adrenoceptors during contractions by electrical field stimulation in mouse vas deferens

  • Alsufyani, Hadeel A.;Docherty, James R.
    • The Korean Journal of Physiology and Pharmacology
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    • v.25 no.6
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    • pp.525-532
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    • 2021
  • We have investigated the relative roles of α1-adrenoceptors and purinoceptors in contractions to low and high frequency stimulation of the mouse vas deferens, in terms of the time course of responses. In separate experiments, isometric contractile responses were obtained to 10 pulses at 1 Hz and 40 pulses at 10 Hz. Responses to 1 Hz stimulation consisted of a series of discrete peaks. The α1A-adrenoceptor antagonist RS100329 (10-9M-10-7M) significantly reduced the response to the first pulse, the α1D-adrenoceptor antagonist BMY7378 (10-7M-10-6M) significantly reduced the response to the first two pulses, and the non-selective α1-adrenoceptor antagonist prazosin (10-8M) reduced the response to the first 4 pulses at 1 Hz. Responses to 10 Hz stimulation consisted of an early peak response and a maintained plateau response. RS100329 significantly reduced the peak response but did not significantly affect the plateau response. Prazosin, significantly reduced both the peak and plateau responses. The α1A-adrenoceptor antagonist RS17053 in high concentrations reduced mainly the plateau response leaving a clear early peak response. The plateau response of contraction was almost abolished by the purinoceptor antagonist suramin. These results suggest that there is a relatively minor early α1D-adrenoceptor and a larger early α1A-adrenoceptor component to stimulationevoked contractions of mouse vas deferens, but the major α1-adrenoceptor component is revealed by prazosin to be α1B-adrenoceptor mediated. α1B-Adrenoceptor activation probably facilitates contractions mediated by other α1-adrenoceptors and by purinoceptors. These results suggest that combined non-selective α1-adrenoceptor blockade, particularly α1B-adrenoceptor blockade, in addition to P2X1-purinoceptor blockade is useful in reducing male fertility.

Low-dose radiation activates Nrf1/2 through reactive species and the Ca2+/ERK1/2 signaling pathway in human skin fibroblast cells

  • Lee, Eun Kyeong;Kim, Jin-Ah;Park, Seong Joon;Kim, Jeung Ki;Heo, Kyu;Yang, Kwang Mo;Son, Tae Gen
    • BMB Reports
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    • v.46 no.5
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    • pp.258-263
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    • 2013
  • In the current study, we explored the effect of LDR on the activation of Nrfs transcription factor involved in cellular redox events. Experiments were carried out utilizing 0.05 and 0.5 Gy X-ray irradiated normal human skin fibroblast HS27 cells. The results showed LDR induced Nrf1 and Nrf2 activation and expression of antioxidant genes HO-1, Mn-SOD, and NQO1. In particular, 0.05 Gy-irradiation increased only Nrf1 activation, but 0.5 Gy induced both Nrf1 and Nrf2 activation. LDR-mediated Nrf1/2 activation was accompanied by reactive species (RS) generation and $Ca^{2+}$ flux. This effect was abolished in the presence of N-acetyl-cysteine and BAPTA- AM. Furthermore, Nrf1/2 activation by LDR was suppressed by PD98059, an inhibitor of ERK1/2. In conclusion, LDR induces Nrf1 and Nrf2 activation and expression of Nrf-regulated antioxidant defense genes through RS and $Ca^{2+}$/ERK1/2 pathways, suggesting new insights into the molecular mechanism underlying the beneficial role of LDR in HS27 cells.

A Robotic Milking Manipulator for Teat-cup Attachment Modules (착유컵 자동 착탈을 위한 매니퓰레이터 개발)

  • Lee, D. W.;Kim, W.;Kim, H. T.;Kim, D. W.;Choi, D. Y.;Han, J. D.;Kwon, D. J.;Lee, S. K.
    • Journal of Biosystems Engineering
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    • v.26 no.2
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    • pp.163-168
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    • 2001
  • A manipulator for test-cup attachment modules, which was a part of a robot milking system, was developed to reduce cost and labor for cow milking processing. A Cartesian coordinate manipulator was designed for the milking process, because it was quite flexible and can be constructed more economically than any other configuration. The manipulator was made use of DC motors, screws for power transmission, a RS422 interface system for the transmission of coordinate values and a one-chip microprocessor, 89C52. Performance tests of the manipulator were conducted to measure experimentally the precision of all axes. Some of the results are as follows. 1. The Cartesian coordinate manipulator was designed and built. Dimension of the three perpendicular axes (X, Y, and Z) and one arm’s axis(W) to pick up and transfer the modules were 700㎜$\times$450㎜$\times$550㎜$\times$650㎜. The arm’s axis moved the teat-cup attachment module, which attached four teat-cup to four teats, detached four teat-cup from four teats, was designed and manufactured by using CAD, CAM and CNC. 3. After 10 replications of exercising the manipulator, mean precision values(positioning error) of X, Y, Z axes wee 0.48㎜, 0.20㎜, 0.19㎜, respectively. Therefore, we conclude the axes to have a precision better than 0.5㎜, had no problem to operate correctly the milking manipulator.

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Design and Implementation of Engineering Qualification Model of S-Band Transmitter for STSAT-3 (과학기술위성 3호 S-대역 송신기 인증모델 설계 및 제작)

  • Oh, Seung-Han;Seo, Gyu-Jae;Oh, Dae-Soo;Lee, Jung-Soo;Oh, Chi-Wook
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.38 no.1
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    • pp.80-86
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    • 2010
  • This paper describes the development result of S-band Transmitter of STSAT-3 by satellite research center(SaTReC), KAIST. STSAT-3 has two kinds of communication channels, S- band for Telemetry & Command and X-band for mission payload. S-band Transmiiter(STX) consist of modulator, frequency synthesizer, power amp and DC/DC converter. The modulation scheme of STX is FSK(Frequency Shift Keying). The interface between spacecraft OBC and STX is RS-422. The STX is based on modular design. The RF output power of STX is 1.5W(31.7dBm) and BER of STX is under 1E-5. The Test of STX is completed successfully such as functional Test and environmental(vibration, thermal vacuum) Test.

Cu/Si/Cu Ohmic contacts to n-type 4H-SiC (n형 4H-SiC의 Cu/Si/Cu 오옴성 접합)

  • 정경화;조남인;김민철
    • Proceedings of the International Microelectronics And Packaging Society Conference
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    • 2002.11a
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    • pp.73-77
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    • 2002
  • Characteristics of Cu/Si/Cu ohmic contacts to n-type 4H-SiC were investigated systematically. The ohmic contacts were formed by rf sputtering of multi layer Cu/Si/Cu sputtered sequentially. The annealings were peformed With 2-Step using RTP in vacuum ambient. The specific contact resistivity($\rho$c), sheet resistance(Rs), contact resistance(Rc), transfer length(L$_{T}$) were calculated from resistance(R$_{T}$) versus contact spacing(d) measurements obtained from TLM(transmission line method) structure. Best results were obtained for a sample annealed at vacuum as $\rho$c = 1.0x10$^{-6}$ $\Omega$$\textrm{cm}^2$, Rc = 2$\Omega$ and L$_{T}$ = 1${\mu}{\textrm}{m}$. The physical properties of contacts were examined using XRO and AES. The results showed that copper silicide was formed on SiC and Cu was migrated into SiC.o SiC.

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Effects of Amylose Content on Properties of Lintnerized Maize Starches and Yield of Resistant Starch (아밀로오스 함량이 산처리 옥수수전분의 특성 및 저항전분수율에 미치는 영향)

  • Lee, Shin-Kyung;Shin, Mal-Shick
    • Applied Biological Chemistry
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    • v.40 no.5
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    • pp.395-399
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    • 1997
  • Lintnerization properties and yield of enzyme-resistant starch in maize starches with different amylose content were investigated. Hydrolytic patterns of starches showed two distinct stages. With decreasing the amylose content, hydrolysis extents of starch increased. X-ray diffraction patterns of Amloca, PFP and commercial maize starch were the A crystalline type, and those of Amaizo 5 and Amylomaize VII were the B crystalline type. As acid hydrolysis time increased, the relative crystallinity of starches increased. After heating-cooling treatment was repeated up to 4 times, the yield of RS increased with increasing the amylose content. The yields of RS in lintnerzed maize starches decreased with acid treatment.

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Design of Mobile Telemedicine System using RFID based on Embedded Linux (임베디드 리눅스 환경에서 RFID 기반의 Mobile Telemedicine System 구현)

  • Yun, Chan-Young
    • Proceedings of the Korea Contents Association Conference
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    • 2006.05a
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    • pp.479-482
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    • 2006
  • Mobile Telemedicine uses common technologies that provide a conduit for information exchange between physicians nurses and patients. In addition to patient records, medical professionals will obtain vital signs and other reference data through Ubiquitous Sensor networks(USN). In this paper about a design and implementation of RFID reader platform that received RFID tag information from patients tags, transmit the data to SBC(Single board computer) based on Intel PXA255 ARM CPU and SBC transmit the data to MySQL server in hospital using by Wireless Internet. his system that based on Embedded Linux is composed of RFID module, ARM processor, RS-232 interface, and Wireless LAN. This paper also provides a brief overview and concept of RFID reader, and proposes the design and implementations of the RFID reader platform used QT and MySQL based on Embedded Linux.

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Design of Force Measuring System for Deburring Using Industrial Robot (산업용로봇을 이용한 디버링을 위한 힘측정시스템 설계)

  • Lee, Gyeong Jun;Kim, Han Sol;Kim, Chong Jin;Kim, Hyeon Min;Kim, Gab Soon
    • Journal of the Korean Society for Precision Engineering
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    • v.32 no.7
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    • pp.653-660
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    • 2015
  • This paper describes the design of the force measuring system for an industrial robot's deburring work. The force measuring system is composed of a three-axis force sensor, a measuring device, a housing and a cover. The three-axis force sensor can detect x-direction force, y-direction force and z-direction force at the same time. The measuring device is designed using DSP(Digital Signal Processor), and have a RS-232 and a RS-485 communication port for sending force data to PC or other controller. As a result of test, the repeatability error and the non-lineality error of the three-axis force sensor are less than 0.03%, and the interference error of the sensor is less than 0.95%. It is thought that the force measuring system can be used for an industrial robot's deburring work.

The Ground Interface Concept of the KOMPSAT-II DLS

  • Lee, Sang-Taek;Lee, Sang-Gyu;Lee, Jong-Tae;Youn, Heong-Sik
    • Proceedings of the KSRS Conference
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    • 2002.10a
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    • pp.228-228
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    • 2002
  • The DLS(Data Link System) is located in the PDTS(Payload Data Transmission Subsystem) of KOMPSAT-II, and its main function is to provide communication link with Ground Segment as a space segment. DLS receive the data of MSC, OBC from DCSU(Data Compression Storage Unit) and transmit to the Ground Station by X-Band RF link. DLS is consist of CCU(Channel Coding Unit), QTX(QPSK Transmitter, ASU(Antenna Switch Unit) CCU makes a packet for communication after several kind of data processing such like Ciphering, RS Coding. QTX transmit PDTS data by OQPSK. Modulation. ASU is the unit for reliability of antenna switching. So, DLS's function is consists of ciphering, RS coding, CCSDS packetizing, randomizing, modulation and switching to antenna. These DLS's functions are controlled by PMU(Payload Management Unit). All commands to DLS are sent by PMU and all telemetries of DLS are sent to the PMU. The PMU receives commands from OBC and sends telemetries to the OBC. The OBC communicates with Ground Station by S-Band RF link. This paper presents the on-orbit DLS operation concept through the ground segment.

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Effects of the Ge Prearmophization Ion Implantation on Titanium Salicide Junctions (게르마늄 Prearmophization 이온주입을 이용한 티타늄 salicide 접합부 특성 개선)

  • Kim, Sam-Dong;Lee, Seong-Dae;Lee, Jin-Gu;Hwang, In-Seok;Park, Dae-Gyu
    • Korean Journal of Materials Research
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    • v.10 no.12
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    • pp.812-818
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    • 2000
  • We studied the effects of Ge preamorphization (PAM) on 0.25$\mu\textrm{m}$ Ti-salicide junctions using comparative study with As PAM. For each PAM schemes, ion implantations are performed at a dose of 2E14 ion/$\textrm{cm}^2$ and at 20keV energy using $^{75}$ /As+and GeF4 ion sources. Ge PAM showed better sheet resistance and within- wafer uniformity than those of As PAM at 0.257m line width of n +/p-well junctions. This attributes to enhanced C54-silicidation reaction and strong (040) preferred orientation of the C54-silicide due to minimized As presence at n+ junctions. At p+ junctions, comparable performance was obtained in Rs reduction at fine lines from both As and Ge PAM schemes. Junction leakage current (JLC) revels are below ~1E-14 A/$\mu\textrm{m}^{2}$ at area patterns for all process conditions, whereas no degradation in JLC is shown under Ge PAM condition even at edge- intensive patterns. Smooth $TiSi_2$ interface is observed by cross- section TEM (X- TEM), which supports minimized silicide agglomeration due to Ge PAM and low level of JLC. Both junction break- down voltage (JBV) and contact resistances are satisfactory at all process conditions.

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