• Korean Journal of Microbiology
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• v.53 no.4
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• pp.286-291
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• 2017

In eukaryotic cells, histone modification is an important mechanism to regulate the chromatin structure. The methylation of the fourth lysine on histone H3 (H3K4) by Set1 complex is one of the various well-known histone modifications. Set1 complex has seven subunits including Swd2, which is known to be important for H2B ubiquitination dependent on H3K4 methylation. Swd2 was reported to regulate Set1's methyltransferase activity by binding to near RNA recognition motif (RRM) domain of Set1 and to act as a component of CPF (Cleavage and Polyadenylation Factors) complex involved in RNA 3' end processing. According to the recent reports, two functions of Swd2 work independently of each other and the lethality of Swd2 knockout strain was known to be caused by its function as a component of CPF complex. In this study, we found that Swd2 could influence the Set1's stability as well as histone methyltransferase activity through the association with RRM domain of Set1. Also, we found that ${\Delta}swd2$ mutant bearing truncated-Set1, which cannot interact with Swd2, lost its lethality and grew normally. These results suggest that the dual functions of Swd2 in H3K4 methylation and RNA 3' end processing are not independent in Saccharomyces cerevisiae.

• Journal of Communications and Networks
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• v.15 no.5
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• pp.527-537
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• 2013

Improving resource utilization has been a hot issue in heterogeneous wireless networks (HWNs). This paper proposes a radio resource management (RRM) method based on access proportion optimization. By considering two or more wireless networks in overlapping regions, users in these regions must select one of the networks to access when they engage in calls. Hence, the proportion of service arrival rate that accesses each network in the overlapping region can be treated as an optimized factor for the performance analysis of HWNs. Moreover, this study considers user mobility as an important factor that affects the performance of HWNs, and it is reflected by the handoff rate. The objective of this study is to maximize the total throughput of HWNs by choosing the most appropriate factors. The total throughput of HWNs can be derived on the basis of a Markov model, which is determined by the handoff rate analysis and distribution of service arrival rate in each network. The objective problem can actually be expressed as an optimization problem. Considering the convexity of the objective function, the optimization problem can be solved using the subgradient approach. Finally, an RRM optimization scheme for HWNs is proposed. The simulation results show that the proposed scheme can effectively enhance the throughput of HWNs, i.e., improve the radio resource utilization.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.21 no.11
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• pp.1311-1317
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• 2010

In this paper, for a uplink space division multiple access system named cooperative spatial multiplexing(CSM), radio resource management(RRM) algorithms are proposed based on sharing uplink channel information among a serving base station(BS) and interfering BSs in a uplink coordinated wireless communication system. A constrained maximum transmit power algorithm is proposed for mobile station(MS) to limit uplink inter-cell interference(ICI). And joint scheduling algorithm among coordinated BSs is proposed to enhance uplink capacity through ICI mitigation by using channel information from interfering BSs. It is shown that the proposed RRM algorithm provides a considerable uplink capacity enhancement by effective ICI mitigation only with moderate complexity.

• Restorative Dentistry and Endodontics
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• v.47 no.2
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• pp.18.1-18.9
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• 2022

Objectives: This study evaluated alterations in neuronal conductivity related to calcium silicate cements (CSCs) by investigating compound action potentials (cAPs) in rat sciatic nerves. Materials and Methods: Sciatic nerves were placed in a Tyrode bath and cAPs were recorded before, during, and after the application of test materials for 60-minute control, application, and recovery measurements, respectively. Freshly prepared ProRoot MTA, MTA Angelus, Biodentine, Endosequence RRM-Putty, BioAggregate, and RetroMTA were directly applied onto the nerves. Biopac LabPro version 3.7 was used to record and analyze cAPs. The data were statistically analyzed. Results: None of the CSCs totally blocked cAPs. RetroMTA, Biodentine, and MTA Angelus caused no significant alteration in cAPs (p > 0.05). Significantly lower cAPs were observed in recovery measurements for BioAggregate than in the control condition (p < 0.05). ProRoot MTA significantly but transiently reduced cAPs in the application period compared to the control period (p < 0.05). Endosequence RRM-Putty significantly reduced cAPs. Conclusions: Various CSCs may alter cAPs to some extent, but none of the CSCs irreversibly blocked them. The usage of fast-setting CSCs during apexification or regeneration of immature teeth seems safer than slow-setting CSCs due to their more favorable neuronal effects.

• Tuberculosis and Respiratory Diseases
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• v.62 no.5
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• pp.406-416
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• 2007

Background: Single nucleotide polymorphisms (SNPs), which consist of a substitution of a single nucleotide pair, are the most abundant form of genetic variations occurring with a frequency of approximately 1 per 1000 base pairs. SNPs by themselves do not cause disease but can predispose humans to disease, modify the extent or severity of the disease or influence the drug response and treatment efficacy. Single nucleotide polymorphisms (SNPs), particularly those within the regulatory regions of the genes often influence the expression levels and can modify the disease. Studies examining the associations between SNP and the disease outcome have provided valuable insight into the disease etiology and potential therapeutic intervention. Traditionally, the genotyping of SNPs has been carried out using polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP), which is a low throughput technique not amenable for use in large-scale SNP studies. Recently, TaqMan real-time PCR chemistry was adapted for use in allelic discrimination assays. This study validated the accuracy and utility of real-time PCR technology for SNPs genotyping Methods: The SNPs in promoter sequence (-37 and -524) of lung cancer suppressor gene, RRM1 (ribonucleotide reductase M1 subunit) with the genomic DNA samples of 89 subjects were genotyped using both real-time PCR and PCR-RFLP. Results: The discordance rates were 2.2% (2 mismatches) in -37 and 16.3% (15 mismatches) in -524. Auto-direct sequencing of all the mismatched samples(17 cases) were in accord with the genotypes read by real-time PCR. In addition, 138 genomic DNAs were genotyped using real-time PCR in a duplicate manner (two separated assays). Ninety-eight percent of the samples showed concordance between the two assays. Conclusion: Real-time PCR allelic discrimination assays are amenable to high-throughput genotyping and overcome many of the problematic features associated with PCR-RFLP.

• Analytical Science and Technology
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• v.28 no.5
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• pp.331-340
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• 2015

Mutations in Fused in Sarcoma (FUS) have been identified in patients with amyotrophic lateral sclerosis (ALS) or Frontotemporal Dementia (FTD). Pathological FUS is mis-localized to cytosol and forms aggregates associated with stress granules (SG), while FUS is normally localized to nucleus. However, it is largely unknown how pathological FUS forms SG-aggregates and which domains are responsible for this process. In this study, we examined cellular localization and aggregation of ALS-linked FUS missense mutants (P525L, R521C, R521H, R521G), analyzed the domains responsible for cytosolic FUS aggregation in HEK293T cells, and confirmed this in cultured mouse neurons. To do this, we firstly generated missense mutants of FUS and then examined their cellular localization. We found that P525L was mostly mis-localized to cytosol and formed FUS-positive SG aggregates while R521C, R521H, or R521G was localized to both nucleus and cytosol. To further characterize the domains required for aggregate formation of cytosolic FUS, we generated different domain-deletion mutants using FUS-∆17 which has a deletion of nuclear localization signal. Interestingly, cytosolic FUS without SYGQ and RGG1 domain or cytosolic FUS without RGG2-ZnF-RGG3 domain did not form FUS-positive SG aggregates, while cytosolic FUS without RRM domain generated more aggregates compared to FUS-∆17. Taken together, these data suggest that SYGQ-RGG1 or RGG2-ZnF-RGG3 domain contributes to formation of cytosolic aggregate, while RRM domain might interfere with FUS aggregation. Therefore, our studies will provide important insight for understanding cellular pathogenesis of neurodegeneration associated with FUS aggregate as well as finding therapeutic targets for ALS or FTD.

• Journal of Animal Science and Technology
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• v.48 no.2
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• pp.143-150
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• 2006

The data were collected in the dairy herd improvement program from January 2000 to July 2005. Test data included 825,157 records of first parity and animals with both parents known were included. This study aimed to describe the effect of incomplete lactation records of latest generation to the change in sire's breeding value using Random Regression model (RRM) in genetic evaluation. Estimation of genetic parameter and breeding value for sire used REMLF90 and BLUPF90 program. The phenotypic value on the number of test day records between group TD11, TD8, TD5, TD2 showed no large differences. For all the group heritability of test day milk yield range from 0.30 to 0.36. However TD2 group showed low heritability the least test day recode on the latest generation. The correlation of above 50% between test day and TD11(0.610), TD8(0.616), TD5(0.661) and TD2(0.682) with different records in latest generation. Sire's rank of breeding value varied widely depending on the records on the number of lactation from start to the latest generation. Study showed that change in breeding value ranked if daughter's test recode more so it should have at least 5 test day records. The use of RRM in dairy cattle genetic evaluation would be desirable if complete lactation records for latest generation daughters of young bulls when selection for proven bulls. Random Regression model (RRM) require at least 5 test-day lactation recode.

• Proceedings of the Korean Information Science Society Conference
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• 2000.10c
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• pp.201-203
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• 2000

VOQ 방법은 입력 큐잉의 저 비용과 출력 큐잉의 고성능이라는 장점을 결합한 효과적인 큐잉방법이다. VOQ를 효과적으로 사용하기 위해서는 셀의 순서를 스케쥴 해주는 스케쥴링 알고리즘이 필요하다. 본 논문에서는 기존의 iSlip, RRM의 round-robin based priority 방식을 따르면서 큐의 현재 상태에 따라서 스케쥴링을 달리하는 알고리즘을 제시하였다. 이 알고리즘에서는 큐에 대한 threshold값을 설정하고 큐에 저장하고 있는 셀의 수가 threshold값 보다 클 경우 그 큐의 셀을 연속해서 포워딩 하도록 하였다. 시뮬레이션을 통해 본 논문에서 제시한 알고리즘과 기존의 iSlip 알고리즘과의 성능을 패킷의 지연시간 측면에서 비교해 보았다.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.23 no.2
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• pp.196-206
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• 2012

AESA radar is able to instantaneously and adaptively position and control the beam, and such adaptive beam pointing of AESA radar enables to remarkably improve the multi-mission capability, compared with mechanically scanned array radar. AESA radar brings a new challenges, radar resource management(RRM), which is a technique efficiently allocating finite resources, such as energy and time to each task in an optimal and intelligent way. Especially radar beam scheduling is the most critical component for the success of RRM. In this paper, we proposed stochastic radar beam scheduling algorithm using simulated annealing(SA), and evaluated the performance on the multi-function radar scenario. As a result, we showed that our proposed algorithm is superior to previous dispatching rule based scheduling algorithm from the viewpoint of beam processing latency and the number of scheduled beams, with real time capability.

• Asian-Australasian Journal of Animal Sciences
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• v.28 no.10
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• pp.1407-1418
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• 2015

A total of 32,817 test-day milk yield (TDMY) records of the first lactation of 4,056 Girolando cows daughters of 276 sires, collected from 118 herds between 2000 and 2011 were utilized to estimate the genetic parameters for TDMY via random regression models (RRM) using Legendre's polynomial functions whose orders varied from 3 to 5. In addition, nine measures of persistency in milk yield ($PS_i$) and the genetic trend of 305-day milk yield (305MY) were evaluated. The fit quality criteria used indicated RRM employing the Legendre's polynomial of orders 3 and 5 for fitting the genetic additive and permanent environment effects, respectively, as the best model. The heritability and genetic correlation for TDMY throughout the lactation, obtained with the best model, varied from 0.18 to 0.23 and from -0.03 to 1.00, respectively. The heritability and genetic correlation for persistency and 305MY varied from 0.10 to 0.33 and from -0.98 to 1.00, respectively. The use of $PS_7$ would be the most suitable option for the evaluation of Girolando cattle. The estimated breeding values for 305MY of sires and cows showed significant and positive genetic trends. Thus, the use of selection indices would be indicated in the genetic evaluation of Girolando cattle for both traits.