• Journal of Chest Surgery
• /
• v.20 no.2
• /
• pp.241-250
• /
• 1987

We scrutinized the preoperative electrocardiographic and hemodynamic findings in adult atrial septal defects older than 15 years, and then followed up the postoperative electrocardiographic changes sequentially. In preoperative electrocardiographs, the mean PR interval [0.17 sec] was prolonged than normal adults [M;0.13,F;0.15], and the mean QRS axis [93.1*] was deviated to rightward than normal [M;63.7*,F;64.4*], and 122 cases of all 159 patients [77.8%] were in RAD quadrant. The QRS morphology was classified into three groups; a] crista supraventricularis hypertrophy, 25 cases, b] right ventricular outflow tract hypertrophy, 89 cases, c] right ventricular hypertrophy, 44 cases, and normal rs pattern, 1 case. Comparing the QP/QS, Pp/Ps, Rp/Rs in these three groups, Qp/Qs increased a] 2.65 to b] 2.97 and decreased b] 2.97 to c] 2.55, Pp/Ps increased a] 0.27 to b] 0.35 to c] 0.44, and Rp/Rs increased a] 0.1 to b] 0.14 to c] 0.2. In comparing the atrial fibrillation with sinus rhythm, the patient`s mean age was increased [26.4 to 45.7], the mean Qp/Qs was decreased [2.97 to 2.7], the mean Pp/Ps was increased [0.35 to 0.46], the mean Rp/Rs increased [0.14 to 0.2], and the QRS morphology was RVOT hypertrophy;7 cases, RVH;2 cases in all 11 cases. Therefore, the atrial fibrillation was appeared in progressed status. Increasing the mean pulmonary arterial pressure, size of the R` wave in Vl lead increased, and the QRS morphology tended to become severe patterns. Postoperatively, the PR interval shortened and QRS axis tended to normal axis quadrant, and size of R` wave decreased sequentially, atrial fibrillation disappeared in 4 cases. Conclusively, by use of the conventional surface electrocardiography, we could anticipate the hemodynamic changes and the prognosis at outpatient department.

• Development and Reproduction
• /
• v.28 no.2
• /
• pp.47-54
• /
• 2024

In eukaryotes, RNA splicing, an essential biological process, is crucial for precise gene expression. Inaccurate RNA splicing can cause aberrant mRNA production, disrupting protein synthesis. To regulate splicing efficiency, some splicing factors are reported to undergo Ubiquitin-like Modifier (SUMO)ylation. Our data indicate that in Saccharomyces cerevisiae, the SUMO protease, Ulp2, is involved in splicing. In the ulp2Δ mutant, some ribosomal protein (RP) transcripts exhibited a significant increase in the levels of intron-containing pre-mRNA because of improper splicing. Moreover, we confirmed Ulp2 protein binding to the intronic regions of RP genes. These findings highlight a critical Ulp2 role in RP transcript splicing.

• Proceedings of the Korean Society of Precision Engineering Conference
• /
• 2007.11a
• /
• pp.523-524
• /
• 2007

• 대한핵의학회:학술대회논문집
• /
• 1988.05a
• /
• pp.129.2-130
• /
• 1988

• Food Science of Animal Resources
• /
• v.40 no.1
• /
• pp.87-95
• /
• 2020

In this study, we separated and purified lipase inhibitory peptide from fermented milk by Lactobacillus plantarum Q180 with the aim of developing a new functional anti-lipase activity yogurt product. L. plantarum 180 was inoculated into 10% reconstituted skimmed milk and incubated at 37℃ until the pH of the culture reached pH 4.4. The lipase activity was measured using porcine pancreatic lipase. The lipase inhibitory peptides were gradually isolated by ultrafiltration, reversed phase column chromatography (RPC), reversed phase high-performance liquid chromatography (RP-HPLC), and gel permeation high-performance liquid chromatography (GP-HPLC) from the fermented milk by L. plantarum Q180. An ODS-AQ column was used for the RPC, a Vydac C18 column for the RP-HPLC, and a Superdex Peptide HR column for the GP-HPLC. The peptide was composed of Asp, Thr, Ile, Ser, Ala, and Gln, and the anti-lipase activity (IC₅₀) was 2,817 ㎍/mL.

• Proceedings of the Korean Society For Composite Materials Conference
• /
• 2002.10a
• /
• pp.179-182
• /
• 2002

Rapid Prototyping (RP) technologies provide the ability to fabricate initial prototypes from various model materials. Stratasys' Fused Deposition Modeling (FDM) is a typical RP process that can fabricate prototypes out of plastic materials, and the parts made from FDM were often used as load-carrying elements. Because FDM deposits materials in about $300\mutextrm{m}$ thin filament with designated orientation, parts made from FDM show anisotropic material properties. This paper proposes an analytic model to predict the tensile strength of FDM parts. Applying the Classical Lamination Theory, which was developed for laminated composite materials, a computer code was implemented. Tsai-Wu failure criterion was added to the code to predict the failure of the FDM parts. The tensile strengths predicted by the analytic model were compared with experimental data. The data and prediction agreed reasonably well to prove the validity of the model. In addition, a web-based advisory service was developed to provide to strength prediction and design rules for FDM parts.

• Archives of Pharmacal Research
• /
• v.17 no.5
• /
• pp.360-363
• /
• 1994

Column--swtiching technique with a reversed-phase high performance liquid chromatographic method has been developed for the routine analysis of radioiodinated insulin and its degadation products in biological fluids. The diluted biological samples were loaded onto a precolumn packed with LiChrosorb RP-8 $(25-40{\;}{\mu}m)$ using 0.1% trifuoroacetic acid (TFA) in water as a washing solvent. After valve switching, the concentrated insulins were eluted in the back-flush mode and separated by a W-Porex $C_{18}$ column with a gradient of 0.1% TFA in water and 0.1% TFA in acetonitrile as the mobile phase. The method showed good precision, accuracy and speed with the detection limit of 20 pg/ml. Total analysis time per sample was about 40 min and the coefficients of variation were less than 8, 2%.

• Analytical Science and Technology
• /
• v.8 no.4
• /
• pp.859-864
• /
• 1995

Mono- and oligosaccharides are derivatized with p-aminobenzoic ethyl ester (ABEE), strongly absorbs UV light at 254 nm, in the presence of sodium cyanoborohydride. C18-bonded silica column is used for the separation of sugar-ABEE derivatives in an isocratic mode. RP-HPLC conditions are optimized by using ternary mixture as mobile phase and $45^{\circ}C$ as a column temperature. Sugar-ABEE derivatives are separated well within a short run time (ca. 25 min) by reverse-phase partition chromatographic mode. The ($1{\rightarrow}6$) linkage type of dihexose-ABEE derivatives has shorter retention time than ($1{\rightarrow}4$)-linkage type. After acid hydrolysis of glycoproteins with 2M trifluoroacetic acid, monosaccharide composition and contents are determined. This procedure is very useful for the simultaneous analysis of neutral and amino sugars in a single chromatographic step using RP-HPLC without reacetylation of deacetylated amino sugars, which are produced by acid hydrolysis.

• Natural Product Sciences
• /
• v.24 no.4
• /
• pp.288-292
• /
• 2018

High-performance countercurrent chromatography (HPCCC) coupled with reversed-phase highperformance liquid chromatography (RP-HPLC) method was developed to isolate dihydrophaseic acid 3'-O-${\beta}$-D-glucopyranoside (DHPAG) from the extract of Nelumbo nucifera seeds. Enriched DHPAG sample (2.3 g) was separated by HPCCC using ethyl acetate/n-butanol/water system (6:4:10, v/v/v, normal-phase mode, flow rate: 4.0 mL/min) to give 23.1 mg of DHPAG with purity of 88.7%. Further preparative RP-HPLC experiment gave pure DHPAG (16.3 mg, purity > 98%). The current study demonstrates that utilization of CCC method maximizes the isolation efficiency compared with that of solid-based conventional column chromatography.

• Proceedings of the Korea Information Processing Society Conference
• /
• 2009.11a
• /
• pp.459-460
• /
• 2009

과거 네트워크는 물리적 회선을 이용한 유선 네트워크 기반의 연구를 통하여 각 노드간의 통신을 수행하였다. 하지만 시대가 흐름에 따라 통신환경이 유선 네트워크에서 무선 네트워크 기반의 통신으로 이동하였으며 그에 관한 연구가 활발히 진행되고 있다. 특히 clustering routing 기법은 무선 네트워크 환경에서의 한계였던 에너지 소모 문제를 보완하는 기법으로서 각광받고 있다. LS2RP는 이동성이 심한 Ad Hoc 네트워크 환경에서 CH가 backup을 통해 해당 cluster를 벗어날 경우를 대비한다. 하지만 CH가 cluster를 벗어난 이후 data chunk 결합을 통한 ID database 복구와 CH 재선정을 수행하기 때문에 그동안 network의 사용이 불가능해진다는 문제점을 가지고 있다. 따라서 본 논문에서는 CH의 이동을 예측하여 미리 sub CH를 선정하여 네트워크의 안정성을 강화하는 기법을 제시한다. 시뮬레이션 수행 결과 이동성에 따른 control traffic 측면에서 기존의 LS2RP에 비해 12% 더 높은 performance를 보이고 있음을 알 수 있었다.