Development and Reproduction (한국발생생물학회지:발생과생식)

The Korean Society of Developmental Biology (한국발생생물학회)
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Yeast Small Ubiquitin-Like Modifier (SUMO) Protease Ulp2 is Involved in RNA Splicing

  • Jeong-Min Park (KNU G-LAMP Project Group, KNU Institute of Basic Sciences, School of Life Sciences, BK21 FOUR KNU Creative BioResearch Group, College of Natural Sciences, Kyungpook National University) ;
  • Seungji Choi (Department of Biomedical Sciences, Korea University College of Medicine) ;
  • Dong Kyu Choi (KNU G-LAMP Project Group, KNU Institute of Basic Sciences, School of Life Sciences, BK21 FOUR KNU Creative BioResearch Group, College of Natural Sciences, Kyungpook National University) ;
  • Hyun-Shik Lee (KNU G-LAMP Project Group, KNU Institute of Basic Sciences, School of Life Sciences, BK21 FOUR KNU Creative BioResearch Group, College of Natural Sciences, Kyungpook National University) ;
  • Dong-Hyung Cho (KNU G-LAMP Project Group, KNU Institute of Basic Sciences, School of Life Sciences, BK21 FOUR KNU Creative BioResearch Group, College of Natural Sciences, Kyungpook National University) ;
  • Jungmin Choi (Department of Biomedical Sciences, Korea University College of Medicine) ;
  • Hong-Yeoul Ryu (KNU G-LAMP Project Group, KNU Institute of Basic Sciences, School of Life Sciences, BK21 FOUR KNU Creative BioResearch Group, College of Natural Sciences, Kyungpook National University)
  • Received : 2024.03.15
  • Accepted : 2024.05.20
  • Published : 2024.06.30
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Abstract

In eukaryotes, RNA splicing, an essential biological process, is crucial for precise gene expression. Inaccurate RNA splicing can cause aberrant mRNA production, disrupting protein synthesis. To regulate splicing efficiency, some splicing factors are reported to undergo Ubiquitin-like Modifier (SUMO)ylation. Our data indicate that in Saccharomyces cerevisiae, the SUMO protease, Ulp2, is involved in splicing. In the ulp2Δ mutant, some ribosomal protein (RP) transcripts exhibited a significant increase in the levels of intron-containing pre-mRNA because of improper splicing. Moreover, we confirmed Ulp2 protein binding to the intronic regions of RP genes. These findings highlight a critical Ulp2 role in RP transcript splicing.

Keywords

Acknowledgement

This study was supported by the National Research Foundation of Korea (NRF) grant funded by the South Korea government (MSIT) [RS-2023-00212894], Learning & Academic research institution for Master's·Ph.D. students, and Postdocs (LAMP) Program of the National Research Foundation of Korea (NRF) grant funded by the Ministry of Education [RS-2023-00301914], Korea Institute for Advancement of Technology funded by the Ministry of Trade, Industry and Energy [P0025489], and Korean Environment Industry & Technology Institute (KEITI) through Core Technology Development Project for Environmental Diseases Prevention and Management funded by Korean Ministry of Environment (MOE) (no. 2022003310001).

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