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http://dx.doi.org/10.5851/kosfa.2019.e87

Separation and Purification of Lipase Inhibitory Peptide from Fermented Milk by Lactobacillus plantarum Q180  

Kim, Seulki (Fermented Food Research Team, Korea Food Research Institute)
Lim, Sang-Dong (Fermented Food Research Team, Korea Food Research Institute)
Publication Information
Food Science of Animal Resources / v.40, no.1, 2020 , pp. 87-95 More about this Journal
Abstract
In this study, we separated and purified lipase inhibitory peptide from fermented milk by Lactobacillus plantarum Q180 with the aim of developing a new functional anti-lipase activity yogurt product. L. plantarum 180 was inoculated into 10% reconstituted skimmed milk and incubated at 37℃ until the pH of the culture reached pH 4.4. The lipase activity was measured using porcine pancreatic lipase. The lipase inhibitory peptides were gradually isolated by ultrafiltration, reversed phase column chromatography (RPC), reversed phase high-performance liquid chromatography (RP-HPLC), and gel permeation high-performance liquid chromatography (GP-HPLC) from the fermented milk by L. plantarum Q180. An ODS-AQ column was used for the RPC, a Vydac C18 column for the RP-HPLC, and a Superdex Peptide HR column for the GP-HPLC. The peptide was composed of Asp, Thr, Ile, Ser, Ala, and Gln, and the anti-lipase activity (IC50) was 2,817 ㎍/mL.
Keywords
separation; purification; lipase inhibitory peptide; fermented milk; Lactobacillus plantarum;
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