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http://dx.doi.org/10.20307/nps.2018.24.4.288

Efficient Isolation of Dihydrophaseic acid 3'-O-β-D-Glucopyranoside from Nelumbo nucifera Seeds Using High-performance Countercurrent Chromatography and Reverse-phased High-performance Liquid Chromatography  

Rho, Taewoong (College of Pharmacy and Integrated Research Institute of Pharmaceutical Sciences, The Catholic University of Korea)
Yoon, Kee Dong (College of Pharmacy and Integrated Research Institute of Pharmaceutical Sciences, The Catholic University of Korea)
Publication Information
Natural Product Sciences / v.24, no.4, 2018 , pp. 288-292 More about this Journal
Abstract
High-performance countercurrent chromatography (HPCCC) coupled with reversed-phase highperformance liquid chromatography (RP-HPLC) method was developed to isolate dihydrophaseic acid 3'-O-${\beta}$-D-glucopyranoside (DHPAG) from the extract of Nelumbo nucifera seeds. Enriched DHPAG sample (2.3 g) was separated by HPCCC using ethyl acetate/n-butanol/water system (6:4:10, v/v/v, normal-phase mode, flow rate: 4.0 mL/min) to give 23.1 mg of DHPAG with purity of 88.7%. Further preparative RP-HPLC experiment gave pure DHPAG (16.3 mg, purity > 98%). The current study demonstrates that utilization of CCC method maximizes the isolation efficiency compared with that of solid-based conventional column chromatography.
Keywords
Nelumbo nucifera; Dihydrophaseic acid 3'-O-${\beta}$-D-glucopyranoside; High-performance countercurrent chromatography; Isolation efficiency;
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