Analytical Science and Technology (분석과학)
- Volume 8 Issue 4
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- Pages.859-864
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- 1995
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- 1225-0163(pISSN)
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- 2288-8985(eISSN)
Determination of Mono- and Oligosaccharides Derivatized with p-Aminobenzoic Ethyl Ester by Reverse Phase HPLC
- Kwon, Hyokjoon (Analytical Sciences Group, Specialty Chemicals Research, LG Chem Research Park) ;
- Kim, Joon (Analytical Sciences Group, Specialty Chemicals Research, LG Chem Research Park)
- Published : 1995.12.25
Abstract
Mono- and oligosaccharides are derivatized with p-aminobenzoic ethyl ester (ABEE), strongly absorbs UV light at 254 nm, in the presence of sodium cyanoborohydride. C18-bonded silica column is used for the separation of sugar-ABEE derivatives in an isocratic mode. RP-HPLC conditions are optimized by using ternary mixture as mobile phase and