• Asian-Australasian Journal of Animal Sciences
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• 제31권8호
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• pp.1098-1102
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• 2018

Objective: Temperament can be defined as a type of behavioral tendency that appears in a relatively stable manner in responses to various external stimuli over time. The aim of this study was to estimate genetic parameters for the records of temperament testing that are used to improve the temperament of Jeju crossbred (Jeju${\times}$Thoroughbred) horses. Methods: This study was conducted using 205 horses (101 females and 104 males) produced between 2010 and 2015. The experimental animals were imprinted and tamed according to the Manual for Horse Taming and Evaluation for Therapeutic Riding Horses and evaluated according to the categories for temperament testing (gentleness, patience, aggressiveness, sensitivity, and friendliness) between 15 months and 18 months of age. Each category was scored on a five-point linear scale. Genetic parameters for the test categories were analyzed using a multi-trait mixed model with repeated records. The ASReml program was used to analyze the data. Results: The heritability of gentleness, patience, aggressiveness, sensitivity and friendliness ranged from 0.08 to 0.53. The standard errors of estimated heritability ranged from 0.13 to 0.17. The test categories showed high genetic correlations with each other, ranging from 0.96 to 0.99 and high repeatability, ranging from 0.70 to 0.73. Conclusion: The results of this study showed that the test categories had moderate heritability and high genetic correlations, but additional studies may be necessary to use the results for the improvement programs of the temperament of Jeju crossbred horses.

• 한국육종학회지
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• 제43권5호
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• pp.368-374
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• 2011

Apricot (Prunus armeniaca L.) cultivars show a gametophytic self-incompatibility (GSI) system, like other fruit species of Rosaceae family. Thus, it is necessary to determine their S-genotypes in order for stable fruit set in commercial cultivation. S-genotypes of apricots were determined by PCR and test crosses. Three sets of consensus primers designed from Prunus S-RNases were used to amplify fragments containing the first and second S-RNase intron, respectively. Through the results obtained from the 3 PCRs, we could identify SI genotypes of 33apricot cultivars. Several cultivars such as 'Heiwa', 'Yamagata No.3' and 'Shinsuoomi' had the self-compatible (Sc) allele. Self-pollination tests revealed that cultivars with Sc allele were self-compatible. Cross-pollination tests confirmed that there was cross-incompatibility between the cultivars with the same S-genotypes. These results might be very useful for growers for effective pollination and for breeders using these in cross breeding programs.

• 한국수정란이식학회지
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• 제28권3호
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• pp.207-213
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• 2013

This study was carried out to examine a molecular marker system for parentage test in Jeju Black cattle (JBC). Based on the preliminarily studies, we finally selected for construction of a novel genetic marker system for molecular traceability, identity test, breed certification, and parentage test in JBC and its related industrial populations. The genetic marker system had eight MS markers, five indel markers, and two single nucleotide polymorphisms (SNPs; g.G299T and g.del310G) within MC1R gene which is critical to verify the breed specific genotypes for coat color of JBC differing from those of exotic black cattle breeds such as Holstein and Angus. The results showed lower level of a combined non-exclusion probability for second parent (NE-P2) of $4.1202{\times}10^{-4}$ than those previously recommended by International Society of Animal Genetics (ISAG) of $5.000{\times}10^{-4}$ for parentage, and a combined non-exclusion probability for sib identity (NE-SI) of $2.679{\times}10^{-5}$. Parentage analysis has been successfully identified the JBC offspring in the indigenous population and cattle farms used the certified AI semens for production using the JBC-derived offspring for commercial beef. This combined molecular marker system will be helpful to supply genetic information for parentage test and traceability and to develop the molecular breeding system for improvement of animal productivity in JBC population.

• 한국약용작물학회지
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• 제27권6호
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• pp.404-411
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• 2019

Background: In September 2017, wilting and rhizome rot symptoms were observed on Atractylodes macrocephala Koidz. in Jecheon-si and Eumseong-gun. This study was carried out to isolate hitherto unidentified pathogenic fungi from A. macrocephala and to test the pathogenicity of isolated fungi against Atractylodes spp. genus such as A. macrocephala, A. japonica, and their interspecific hybrids. Methods and Results: The diseased plants were washed with running tap water, and the boundary between the healthy area and the diseased area was cut while the pathogens were isolated by growing cultures from the diseased areas on Phytophthora semi-selective medium. The internal transcribed spacer (ITS) region of the isolates was used in this study for identification. Test plants were cultivated in the glasshouse at 20℃ - 30℃ for 4 months and then used for pathogenicity test. The pots with plants inoculated with mycelial plugs and zoospores were placed at 25℃ for 48 h in a dew chamber where relative humidity was above 95%, and then moved into the glasshouse at 20℃ - 30℃. The presence or absence of pathogenicity of the strains was determined by evaluating the symptom of plant wilting. The inoculation test was performed in three replicates with a non-treated control. Conclusions: On the basis of results of ITS sequence analysis, the strains isolated from the diseased plants was identified as Phytophthora sansomeana. Biological assay using test plants confirmed the pathogenicity of P. sansomeana against Atractylodes macrocephala. This is the first report of rhizome rot in A. macrocephala caused by P. sansomeana.

• Reproductive and Developmental Biology
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• 제32권2호
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• pp.127-133
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• 2008

The objective of this study was two folds: to investigate the relationship between paternal identification rate and sperm quality parameters such as motility and sperm chromatin structure assay after heterospermic insemination; to see if mutual complement between tests and development of useful technique to enhance the fertility in artificial insemination. In individual boar's fertilizing ability, 3 high fertility boars showed significantly high fertility (p<0.05) compared to 3 low fertility boars, but there was no difference in litter size between two groups. Sperm motility test in pooled and individual semen using computer assisted sperm analysis (CASA) revealed that no significant difference among boars. The high fertile boar showed tendency of low %Red (High red fluorescence/green+red fluorescence) in sperm chromatin structure assay (SCSA) but paternal identification rate from piglets did not differ after heterospermic insemination. The correlation coefficient between individual or pooled semen function test and farrowing rates were well correlated as follows: %Red with litter size (r= - 0.53, p=0.03); %Red with paternal identification rates (r=-0.51, p=0.03); paternal identification rates with litter size (r=0.57, p=0.02). These results indicate that sperm chromatin structure assay and sperm quality parameter test in pooled semen are useful method to predict and evaluate the fertilizing capacity after heterospermic insemination in boars.

• 농업과학연구
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• 제41권4호
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• pp.259-264
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• 2014

This research was conducted to find design factors of seed metering device for developing seeder. It can be sowed precisely 1~3 seeds of Foxtail millet & Sorghum. To obtain fundamental information for designing seed metering device, we conducted adaptability test of the existing seeder in Foxtail millet & Sorghum. Major findings were as followings. Except of Model-A which was adapted sorghum(by seeding metering cup of width 3.9mm and length 4.5mm), seeders which were used in experiment showed that high value of miss-planted rates and more than four-planted rates. So to enhance precision of seeding in Foxtail millet & Sorghum, existing seeders were considered necessary by some supplementation.

• 한국작물학회지
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• 제47권1호
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• pp.36-41
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• 2002

Two rice varieties, 'Oochikara' with large grain and 'Hwayeongbyeo' and their progenies (F$_1$, F$_2$, B$_1$ and B$_2$) were tested to understand gene action of morphological traits of rice grain and their relationships. The evaluated traits were 1,000-grain weight, grain length, width, thickness, length-width ratio and chalkiness of brown rice. Correlation between grain weight and chalkiness was highly significant in the all progenies, and grain length were not associated with width and thickness in an F$_2$ population. Scaling test and jonit scaling test revealed that inheritance of grain traits were fitted to additive-dominance model without epistasis. Additive effects for the traits were much greater than the dominance effects.

• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2010년도 심포지엄 및 추계학술발표회
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• pp.139-139
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• 2010

• 농약과학회지
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• 제12권3호
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• pp.229-235
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• 2008

시설재배 딸기에 사용하는 농약이 화분매개를 위해 투입된 꿀벌(Apis mellifera L.)에 미치는 영향을 조사하기 위해 꿀벌에 노출될 가능성이 있는 21종의 농약을 선정하여 꿀벌 급성독성시험과 엽상잔류독성시험을 실시하였다. 표준살포농도로 분무처리한 급성접촉독성시험에서는 24시간 후에 꿀벌치사율이 대조군과 유한 차이를 보인 농약은 dichlofluanid WP 등 6종이었고, 48시간 후에는 fenpropathrin EC 등 7종이었다. 급성 섭식독성시험에서는 fenpropathrin EC만이 꿀벌에 독성을 보여주었으며 milbemectin EC는 배량처리군에서 대조군과 비교하여 유의한 치사율 차이를 보여주었다. 또한 엽상잔류독성시험에서는 fenpropathrin EC만이 10일 이상 장기간 잔류독성을 보였다. 이상의 결과를 종합하여 21종 제품농약에 대한 살포 후 꿀벌안전방사기간을 제안하였다.

• Journal of Biosystems Engineering
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• 제35권1호
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• pp.15-20
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• 2010

Tractor PTO output and fuel consumption rate under the korean paddy and various paddy operations were measured and analyzed, in which all the measurements were accomplished by the OECD tractor test codes and the collected information will be utilized for defining tractor energy efficiency class and its test methods. Tractor PTO performance tests were conducted under full-load, part-load and various engine RPMs with part-load at the engine laboratory, while the paddy operations were dry land plowing, wet and dry land rotary tilling and wet land preparation under various soils. As a whole, the rated tractor outputs were ranged from 17% to 100% in the various tillage and land preparation operations, however, the loads for the paddy operations of 1,700 to 2,000 rpm were very close to the OECD tractor load distribution thus it would be appropriate to adopt OECD tractor test codes to measure energy consumption efficiency of tractor.