Korean Journal of Breeding Science (한국육종학회지)

The Korean Society of Breeding Science (한국육종학회)
권호 표지

Identification of Self-incompatibility Genotypes of Apricot (Prunus armeniaca L.) by PCR and Test Crosses

  • Jun, Ji Hae (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Nam, Eun Young (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Kwon, Jung Hyun (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Chung, Kyeong Ho (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Yoon, Ik-Koo (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Yun, Seok-Kyu (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Shin, Yong-Uk (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Kwon, Soon Il (Apple Research Station, National Institute of Horticultural & Herbal Science, RDA)
  • Received : 2011.10.12
  • Accepted : 2011.11.30
  • Published : 2011.12.30
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Abstract

Apricot (Prunus armeniaca L.) cultivars show a gametophytic self-incompatibility (GSI) system, like other fruit species of Rosaceae family. Thus, it is necessary to determine their S-genotypes in order for stable fruit set in commercial cultivation. S-genotypes of apricots were determined by PCR and test crosses. Three sets of consensus primers designed from Prunus S-RNases were used to amplify fragments containing the first and second S-RNase intron, respectively. Through the results obtained from the 3 PCRs, we could identify SI genotypes of 33apricot cultivars. Several cultivars such as 'Heiwa', 'Yamagata No.3' and 'Shinsuoomi' had the self-compatible (Sc) allele. Self-pollination tests revealed that cultivars with Sc allele were self-compatible. Cross-pollination tests confirmed that there was cross-incompatibility between the cultivars with the same S-genotypes. These results might be very useful for growers for effective pollination and for breeders using these in cross breeding programs.

Keywords

References

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