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Identification of Self-incompatibility Genotypes of Apricot (Prunus armeniaca L.) by PCR and Test Crosses  

Jun, Ji Hae (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA)
Nam, Eun Young (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA)
Kwon, Jung Hyun (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA)
Chung, Kyeong Ho (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA)
Yoon, Ik-Koo (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA)
Yun, Seok-Kyu (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA)
Shin, Yong-Uk (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA)
Kwon, Soon Il (Apple Research Station, National Institute of Horticultural & Herbal Science, RDA)
Publication Information
Korean Journal of Breeding Science / v.43, no.5, 2011 , pp. 368-374 More about this Journal
Abstract
Apricot (Prunus armeniaca L.) cultivars show a gametophytic self-incompatibility (GSI) system, like other fruit species of Rosaceae family. Thus, it is necessary to determine their S-genotypes in order for stable fruit set in commercial cultivation. S-genotypes of apricots were determined by PCR and test crosses. Three sets of consensus primers designed from Prunus S-RNases were used to amplify fragments containing the first and second S-RNase intron, respectively. Through the results obtained from the 3 PCRs, we could identify SI genotypes of 33apricot cultivars. Several cultivars such as 'Heiwa', 'Yamagata No.3' and 'Shinsuoomi' had the self-compatible (Sc) allele. Self-pollination tests revealed that cultivars with Sc allele were self-compatible. Cross-pollination tests confirmed that there was cross-incompatibility between the cultivars with the same S-genotypes. These results might be very useful for growers for effective pollination and for breeders using these in cross breeding programs.
Keywords
Breeding; Cultivar; Pollinizer; S-allele; S-RNase;
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Times Cited By KSCI : 2  (Citation Analysis)
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