• Title/Summary/Keyword: R9

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Identification of Secondary Metabolites from the Stems of Viburnum erosum (덜꿩나무(Viburnum erosum)줄기로부터 이차대사산물의 분리 및 동정)

  • In, Seo-Ji;Seo, Kyeong-Hwa;Song, Na-Young;Song, Myoung-Chong;Baek, Nam-In
    • Journal of Applied Biological Chemistry
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    • v.57 no.2
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    • pp.165-170
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    • 2014
  • The stems of Viburnum erosum were extracted with 80% MeOH. The concentrated extract was partitioned with EtOAc, n-BuOH, and $H_2O$. From the EtOAc and n-BuOH fractions, four compounds were isolated through the repeated $SiO_2$, octadecyl silica gel, and Sephadex LH-20 column chromatographies. Based on NMR, MS, and IR spectroscopic data, the chemical structures were determined as betulinic aldehyde (1), koaburside (2), (6R,7E,9R)-9-hydroxymegastigma-4,7-dien-3-one-9-O-${\beta}$-D-glucopyranoside (3), and byzantionoside B (4). All the compounds were isolated for the first time from the stems of Viburnum erosum.

Effect on Fertilizing Capacity According to Sperm Concentration of Liquid Boar Semen (돼지 액상정액의 정자농도가 번식성적에 미치는 영향)

  • Kim, I.C.;Lee, J.H.;Kim, H.J.;Choi, D.Y.;Son, D.S.;Park, C.S.
    • Korean Journal of Animal Reproduction
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    • v.23 no.4
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    • pp.333-335
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    • 1999
  • This study was carried out to find out the effect on fertilizing capacity according to sperm concentration of liquid boar semen. Four different doses with various motile sperm cells of 3.0$\times$10$^{9}$ , 2.5$\times$10$^{9}$ , 2.0$\times$10$^{9}$ , and $1.5\times$10$^{9}$ per 80$m\ell$ plastic bottle were inseminated twice 12 h interval after standing estrus in 6,818 sows. Farrowing rate and total piglets per litter were 82.2% and 10.9, respectively, with no significant differences among the other treatments. The presumption of optimal concentration of motile sperm cells in the liquid boar semen was best at 2.0~2.3$\times$10$^{9}$ per dose.

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Genetic and molecular analysis of the R-mb gene from maize (옥수수 R-mb 유전자의 유전분석과 그의 구조)

  • 윤필용;유삼규;송원용;윤충효;임용표
    • Korean Journal of Plant Tissue Culture
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    • v.24 no.3
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    • pp.161-165
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    • 1997
  • The R-mb locus of maize is one of several genes that encode tissue-specific transcriptional regulator for the anthocyanin biosynthesis in plant parts and the aleurone layer in seeds. We found that the seed pigment frequencies gradually decreased at selfed progenies of the R-mb genetic stocks. In order to analyze the genomic structure of R-mb locus components, genomic Southern blot was performed by using R specific probe, pR-nj:1. Two bands were detected at the size of about 3.9 and 7.75kb. Five R-mb positive clones (mb-II, III, V,Ⅵ, and Ⅶ) were obtained by screening of maize genomic λFIXII library using R specific probe pR-nj:1. We constructed the restriction map of clone mb-II (7.75Kb positive) and mb-Ⅵ (3.9Kb positive), and have compared these with other R locus genes. From genetic and molecular analysis, it is suggested that R-mb complex consists two copy of R elements, and each element shows the paramutagenic and gene silencing effects by the fashion of cis-inactivation.

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Evaluation of Vi-Passive Hemagglutination, SD$^{(R)}$ Kit, and Widal Test for Serological Diagnosis of Typhoid Fever (장티푸스의 혈청학적 진단을 위한 Vi-수동혈구응집법, SD$^{(R)}$ Kit 및 Widal 시험에 대한 효용성 평가)

  • Kim, Sung-Hun;Kim, Shuk-Ho;Lee, Deog-Yong;Lee, Esther;Park, Mi-Sun;Lee, Bok-Kwon
    • Korean Journal of Microbiology
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    • v.46 no.2
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    • pp.219-222
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    • 2010
  • In this study, we evaluated Vi-passive hemagglutination (Vi-PHA), SD Salmonella Typhi IgG/IgM ($SD^{(R)}$ kit) and Widal test for the rapid laboratory diagnosis of typhoid fever patients. A total of 36 serum samples from febrile patients in Korea from 2005 to 2006 were used. Among 36 patients, 27 were fever patients without typhoid, 9 were typhoid fever. Vi-PHA showed 8 positive results out of 9 typhoid fever patients (sensitivity 88.9%) and 1 positive and 26 negative results out of 27 febrile patients without typhoid (specificity 96.3%). The sensitivity and the specificity of $SD^{(R)}$ kit were 100% and 92.6%, respectively. However, the sensitivity and the specificity of Widal O & H tests were 88.9%, 100%, and 77.8%, 70.4%, respectively. Consequently, Widal H and $SD^{(R)}$ kit showed higher sensitivity and Vi-PHA showed higher specificity. To efficient diagnosis, Vi-PHA may be sufficient diagnosis method in acute cases and $SD^{(R)}$ kit and Widal test may be sufficient in sporadic area and high risk group.

Ultrastructure of the flagellar apparatus in Rhodomonas salina (Cryptophyceae, Cryptophyta)

  • Nam, Seung Won;Jo, Bok Yeon;Shin, Woongghi
    • Korean Journal of Environmental Biology
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    • v.38 no.2
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    • pp.278-288
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    • 2020
  • Rhodomonas salina is a phototrophic marine flagellate. We examined the ultrastructure of R. salina with particular attention to the flagellar apparatus by transmission electron microscopy and compared it with that of other cryptomonads reported previously. The major components of the flagellar apparatus in R. salina were a keeled rhizostyle (Rhs), a striated fibrous root(SR), a SR-associated microtubular root (SRm), a mitochondrion-associated lamella (ML), and three types of microtubular roots (9r, 4r, and 2r). The keeled Rhs originated near the proximal end of the dorsal basal body, passed near the nucleus and dissociated at the posterior end of the cell. The SR and SRm originated between two basal bodies and laterally extended to the right side of the cell. The ML originated between two basal bodies and extended to the left side of the cell. The 9r originated between the ventral basal body and the Rhs and extended toward the anterior dorsal lobe of the cell. The 4r originated near the 9r and extended toward the dorsal lobe with the 2r, which originated between two basal bodies. Here, the flagellar apparatus in R. salina is described, and the ultrastructure of the flagellar apparatus is compared among cryptomonad species.

Phytochemical Constituents of the Leaves of Hosta longipes

  • Kim, Chung Sub;Kim, Ki Hyun;Lee, Kang Ro
    • Natural Product Sciences
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    • v.20 no.2
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    • pp.86-90
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    • 2014
  • Phytochemical investigation of the 80% MeOH extract from the leaves of Hosta longipes resulted in the isolation of sixteen compounds (1 - 16). The structures of the compounds were elucidated by spectroscopic methods to be methyl 10,10-dimethoxydecanoate (1), methyl 10-hydroxy-8E,12Z-octadecadienoate (2), methyl coriolate (3), trans-phytol (4), phytene-1,2-diol (5), phyton (6), (3S,5R,6S,7E,9R)-7-megastigmene-3,6,9-triol (7), (3S,5R,6S,9R)-3,6,9-trihydroxymegastigman-7-ene (8), shikimic acid (9), p-coumaramide (10), trans-N-p-coumaroyltyramine (11), cis-N-coumaroyltyramine (12), tryptophan (13), thymidine (14), adenosine (15), and deoxyadenosine (16). Compound 1 was synthesized, but not yet isolated from natural source, and compounds 2-16 were isolated for the first time from this plant source.

Evaporation kent transfer characteristics of R-290 and R-600a in the horizontal tubes (수평관내 R-290과 R-600a의 증발 열전달 특성)

  • Son, Chang-Hyo
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.9 no.2
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    • pp.264-269
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    • 2008
  • This paper presents the experimental results of evaporation heat transfer coefficients of HC(e.g. R290 and R600a), R-22 as a HCFCs refrigerant in horizontal double pipe heat exchangers, having four different inner diameters of 10.07 mm and 6.54 mm respectively. The experiments of the evaporation process were conducted at mass flux of $35.5{\sim}210.4\;kg/m^2s$ and cooling capacity of $0.95{\sim}10.1\;kW$. The main results were summarized as follows : The average evaporation heat transfer coefficient of R-290 and R-600a was higher value than that of R-22. In comparison with R-22, the evaporation heat transfer coefficient of R-290 and 600a is approximately $56.7{\sim}70.1$ and $46.6{\sim}59.7%$ higher, respectively. In comparison with experimental data and some correlations, the evaporation heat transfer coefficients are well matched with the Kandlikar's correlation regardless of a type of refrigerants and tube diameters.

Analysis of Process Parameters to Improve On-Chip Linewidth Variation

  • Jang, Yun-Kyeong;Lee, Doo-Youl;Lee, Sung-Woo;Lee, Eun-Mi;Choi, Soo-Han;Kang, Yool;Yeo, Gi-Sung;Woo, Sang-Gyun;Cho, Han-Ku;Park, Jong-Rak
    • JSTS:Journal of Semiconductor Technology and Science
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    • v.4 no.2
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    • pp.100-105
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    • 2004
  • The influencing factors on the OPC (optical proximity correction) results are quantitatively analyzed using OPCed L/S patterns. ${\sigma}$ values of proximity variations are measured to be 9.3 nm and 15.2 nm for PR-A and PR-B, respectively. The effect of post exposure bake condition is assessed. 16.2 nm and 13.8 nm of variations are observed. Proximity variations of 11.6 nm and 15.2 nm are measured by changing the illumination condition. In order not to seriously deteriorate the OPC, these factors should be fixed after the OPC rules are extracted. Proximity variations of 11.4, 13.9, and 15.2 nm are observed for the mask mean-to-targets of 0, 2 and 4 nm, respectively. The decrease the OPC grid size from 1 nm to 0.5 nm enhances the correction resolution and the OCV is reduced from 14.6 nm to 11.4 nm. The enhancement amount of proximity variations are 9.2 nm corresponding to 39% improvement. The critical dimension (CD) uniformity improvement for adopting the small grid size is confirmed by measuring the CD uniformity on real SRAM pattern. CD uniformities are measured 9.9 nm and 8.7 nm for grid size of 1 nm and 0.5 nm, respectively. 22% improvement of the CD uniformity is achieved. The decrease of OPC grid size is shown to improve not only the proximity correction, but also the uniformity.