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Evaluation of Vi-Passive Hemagglutination, SD$^{(R)}$ Kit, and Widal Test for Serological Diagnosis of Typhoid Fever  

Kim, Sung-Hun (Division of Enteric Bacterial Infections, Center for Infectious Disease, National Institute of Health)
Kim, Shuk-Ho (Department of Microbiology, School of Medicine, Kyungpook National University)
Lee, Deog-Yong (Division of Enteric Bacterial Infections, Center for Infectious Disease, National Institute of Health)
Lee, Esther (Division of Enteric Bacterial Infections, Center for Infectious Disease, National Institute of Health)
Park, Mi-Sun (Division of Enteric Bacterial Infections, Center for Infectious Disease, National Institute of Health)
Lee, Bok-Kwon (Division of Enteric Bacterial Infections, Center for Infectious Disease, National Institute of Health)
Publication Information
Korean Journal of Microbiology / v.46, no.2, 2010 , pp. 219-222 More about this Journal
Abstract
In this study, we evaluated Vi-passive hemagglutination (Vi-PHA), SD Salmonella Typhi IgG/IgM ($SD^{(R)}$ kit) and Widal test for the rapid laboratory diagnosis of typhoid fever patients. A total of 36 serum samples from febrile patients in Korea from 2005 to 2006 were used. Among 36 patients, 27 were fever patients without typhoid, 9 were typhoid fever. Vi-PHA showed 8 positive results out of 9 typhoid fever patients (sensitivity 88.9%) and 1 positive and 26 negative results out of 27 febrile patients without typhoid (specificity 96.3%). The sensitivity and the specificity of $SD^{(R)}$ kit were 100% and 92.6%, respectively. However, the sensitivity and the specificity of Widal O & H tests were 88.9%, 100%, and 77.8%, 70.4%, respectively. Consequently, Widal H and $SD^{(R)}$ kit showed higher sensitivity and Vi-PHA showed higher specificity. To efficient diagnosis, Vi-PHA may be sufficient diagnosis method in acute cases and $SD^{(R)}$ kit and Widal test may be sufficient in sporadic area and high risk group.
Keywords
serological diagnosis; SD$^{(R)}$ kit; typhoid fever; Vi-PHA; Widal test;
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