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A Simple Method for Determining Residual p-arsanilic Acid in Aquatic Products using EDTA-Assisted Solvent Extraction and LC-MRM

  • Min Kyeong Kwon;Ye Bin Shin;Young Min Kim;Jin Hwan Kim;Ji Seong Kim;Yong Seok Choi
    • Mass Spectrometry Letters
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    • v.14 no.2
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    • pp.36-41
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    • 2023
  • A simple method was developed to determine residual p-arsanilic acid (ASA), an organo-arsenic compound used as a feed additive, in aquatic products (eel, halibut, and shrimp) using EDTA-assisted solvent extraction and LC-MRM. The method was successfully validated in terms of specificity, linearity (coefficient of determination ≥ 0.995), accuracy (recovery or R, 72.72-78.73%), precision (the relative standard deviation of R, 2.08-6.98%), and sensitivity (the lower limit of quantitation, 5 ppb) according to CODEX guidelines (CAC-GL 71-2009). The use of EDTA in the extraction solvent and water with a suitable pH modifier as the reconstitution solvent may be the key factors for successful results. This is the first method that can be used for monitoring residual ASA in aquatic products using LC-MRM and could contribute to establishing a better aquatic product safety management system.

A Study on the Modified Simple Truss Model to Predict the Punching Shear Strength of PSC Deck Slabs (PSC 바닥판의 뚫림전단강도 예측을 위한 단순트러스모델 개선 연구)

  • Park, Woo Jin;Hwang, Hoon Hee
    • Journal of the Korean Society of Safety
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    • v.30 no.5
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    • pp.67-73
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    • 2015
  • In this paper, the simple truss model was modified to predict the punching shear strength of long-span prestressed concrete (PSC) deck slabs under wheel load including the effects of transverse prestressing and long span length between girders. The strength of the compressive zone arounding punching cone was evaluated by the stiffness of inclined strut which was modified by considering aging effective modulus. The stiffness of springs which control lateral displacement of the roller supports consists of the steel reinforcement and prestressing which passed through the punching cone. Initial angle of struts was determined by the experimental observation to compensate for uncertainties in the complexities of the punching shear. The validity of computed punching shear strength by modified simple truss model was shown by comparing with experimental results and the experimental results were also compared with existing punching shear equations to determine level of predictability. The modified simple truss model appeared to better predict the punching shear strength of PSC deck slabs than other available equations. The punching shear strength, which was determined by snap-through critical load of modified simple truss model, can be used effectively to examine punching shear strength of long span PSC deck slabs.

Implementation of LabVIEW®-based Joint-Linear Motion Blending on a Lab-manufactured 6-Axis Articulated Robot (RS2) (LabVIEW® 기반 6축 수직 다관절 로봇(RS2)의 이종 모션 블랜딩 연구)

  • Lee, D.S.;Chung, W.J.;Jang, J.H.;Kim, M.S.
    • Journal of the Korean Society of Manufacturing Technology Engineers
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    • v.22 no.2
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    • pp.318-323
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    • 2013
  • For fast and accurate motion of 6-axis articulated robot, more noble motion control strategy is needed. In general, the movement strategy of industrial robots can be divided into two kinds, PTP (Point to Point) and CP (Continuous Path). Recently, industrial robots which should be co-worked with machine tools are increasingly needed for performing various jobs, as well as simple handling or welding. Therefore, in order to cope with high-speed handling of the cooperation of industrial robots with machine tools or other devices, CP should be implemented so as to reduce vibration and noise, as well as decreasing operation time. This paper will realize CP motion (especially joint-linear) blending in 3-dimensional space for a 6-axis articulated (lab-manufactured) robot (called as "RS2") by using LabVIEW$^{(R)}$ (6) programming, based on a parametric interpolation. Another small contribution of this paper is the proposal of motion blending simulation technique based on Recurdyn$^{(R)}$ V7 and Solidworks$^{(R)}$, in order to figure out whether the joint-linear blending motion can generate the stable motion of robot in the sense of velocity magnitude at the end-effector of robot or not. In order to evaluate the performance of joint-linear motion blending, simple PTP (i.e., linear-linear) is also physically implemented on RS2. The implementation results of joint-linear motion blending and PTP are compared in terms of vibration magnitude and travel time by using the vibration testing equipment of Medallion of Zonic$^{(R)}$. It can be confirmed verified that the vibration peak of joint-linear motion blending has been reduced to 1/10, compared to that of PTP.

N-supplying Capability Evaluation of Corn Field Soils in Pennsylvania (Pennsylvania주 옥수수 재배 토양의 질소공급능력 평가)

  • Hong, Soon-Dal
    • Korean Journal of Soil Science and Fertilizer
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    • v.31 no.4
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    • pp.359-367
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    • 1998
  • In order to determine the nitrogen supplying capabilities (NSC) of corn fields, 47 field experiments were performed in Pennsylvania over 3 year from 1986 and NSCs were estimated by the regression analysis with chemical properties and soil attributes. Although the content of $NO_3-N$ in soil showed the best correlation with NSC ($R^2=0.518$), the standardized partial regression coefficient of $NO_3-N$ for NSC was 0.52, with some variations over the years. This value was slightly higher than those of the other properties which ranged from 0.001 to 0.351. Multiple linear regression with soil attributes for the evaluation of NSC was better than simple regression with $NO_3-N$. The coefficient of determination ($R^2$) for the evaluation of NSC was gradually increased; 0.599 with selected chemical properties, 0.698 with quantitative attributes(chemical properties and depth of Ap horizon), and 0.839 with quantitative and selected qualitative soil attributes. Consequently, in order to evaluate NSC, analysis by multiple linear regression with soil attributes was more reliable and better model than by the simple regression model.

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Simple Design of Equiripple Square Root Pulse Shaping Filter (Square-root 형 등리플 파형성형 필터의 간단한 설계)

  • 황정진;오우진
    • Proceedings of the IEEK Conference
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    • 2001.06d
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    • pp.261-264
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    • 2001
  • In this paper, we introduce a simple design method f9r mot-squared type raised cosine filter with equiripple characteristics. Through some design examples, we show that the proposed filter has much better performance in ripple than the conventional SRCF at the expense of small increasing of ISI. In addition, the proposed Inter is compatible with conventional SRCF. Finally, we designs the filter for W-CDMPI which uses RRC (Root Raised Cosine) with a=0.22, in 12bit finite precision.

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A SIMPLE CHARACTERIZATION OF POSITIVITY PRESERVING SEMI-LINEAR PARABOLIC SYSTEMS

  • Haraux, Alain
    • Journal of the Korean Mathematical Society
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    • v.54 no.6
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    • pp.1817-1828
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    • 2017
  • We give a simple and direct proof of the characterization of positivity preserving semi-flows for ordinary differential systems. The same method provides an abstract result on a class of evolution systems containing reaction-diffusion systems in a bounded domain of ${\mathbb{R}}^n$ with either Neumann or Dirichlet homogeneous boundary conditions. The conditions are exactly the same with or without diffusion. A similar approach gives the optimal result for invariant rectangles in the case of Neumann conditions.

SOME DECOMPOSITION OF MODULAR $sp_4$(F)-MODULES USING DIMENSION FORMULA

  • Kim, Y.K.;Seo, G.S.;Won, S.Y.
    • Bulletin of the Korean Mathematical Society
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    • v.32 no.2
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    • pp.191-200
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    • 1995
  • Simple Lie albegras over algebraically closed field with characteristic p > 7 were classified by H. Strade and R. L. Wilson in 1991 [7]. All modular representations of simple Lie algebras, however, are not classified although some restricted modular representations have been done earlier by Curtis and Steinberg. In connection with this, we would like to decompose basic $sp_4$(F)-modules $sl_4$-(F) and $gl_4$(F) over nonzero characteristic by way of characteristic zero case.

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Electromagnetic Model to Estimate the Vibrations of a Switched Reluctance Machine on the Basis of the Eelctric Power Supply

  • Badreddine, Benabdallah Mohammed
    • Journal of Electrical Engineering and Technology
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    • v.3 no.1
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    • pp.60-67
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    • 2008
  • The vibrations and noise origin in electric material is due to several coupled physical phenomena. The revolving electric machine complete modeling is complex; it does not allow simple parametric machine structure studies for various operation modes. This work presents a simple electromagnetic model which makes possible the machine principal parts flow estimation from flux density. Special interest is given in determining Switched Reluctance Machine (S.R.M) radial acceleration in accordance with the current supply. Our focus will be only on the magnetic origin efforts that are dominating in the S.R.M. The efforts calculation versus the current is presented in the case of a machine with a linearized rate. These efforts are considered as a tangential force producing the torque and a radial force that generates no torque. The application is realized on a 6/4 low power S.R.M type (6 stator teeth and 4 teeth rotor). The mechanical response is substituted in a transfer function. The model takes account of the power supply of the machine, the relation between the current supply and the efforts as well as the vibratory response of the machine to these efforts. Finally, the model is validated by comparison with similar experimental results within the framework of the definite assumptions.

Simple and Rapid Detection of Vancomycin-Resistance Gene from Enterococci by Loop-Mediated Isothermal Amplification

  • Baek, Yun Hee;Hong, Seung Bok;Shin, Kyeong Seob
    • Biomedical Science Letters
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    • v.26 no.3
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    • pp.149-156
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    • 2020
  • We developed a simple and rapid method for detecting vancomycin resistance genes, such as vanA and vanB, using loop-mediated isothermal amplification (LAMP). To identify not only vancomycin resistance genes, but also the genus Enterococcus, primers were designed for vanA, vanB, and 16S rRNA. Screening for vancomycin susceptibility in Enterococcus was performed using Etest (bioMérieux Inc). The results of the LAMP assay were compared to those of real-time RT-PCR. The optimal conditions for the LAMP assay were 65℃ for 60 min. The detection limits of the LAMP assay for vanA, and vanB were 2 × 102 copies/reaction. Compared to RT-PCR, the sensitivities and specificities of LAMP for 16S rRNA, vanA, and vanB were 100/100%, 100/100%, and 100/100%, respectively. The vanA genotype-vanB phenotype accounted for 57.5% (46/80) of the vancomycin-resistant Enterococci samples collected from 2016 to 2019. In conclusion, the LAMP assay developed in this study showed high sensitivity and specificity for vancomycin-resistant genes. Moreover, due to the simplicity and rapidity of the LAMP assay, its use can be very useful in clinical microbiology laboratories.

Investigation of Buffer Traps in AlGaN/GaN Heterostructure Field-Effect Transistors Using a Simple Test Structure

  • Jang, Seung Yup;Shin, Jong-Hoon;Hwang, Eu Jin;Choi, Hyo-Seung;Jeong, Hun;Song, Sang-Hun;Kwon, Hyuck-In
    • JSTS:Journal of Semiconductor Technology and Science
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    • v.14 no.4
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    • pp.478-483
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    • 2014
  • We propose a new method which can extract the information about the electronic traps in the semi-insulating GaN buffer of AlGaN/GaN heterostructure field-effect transistors (HFETs) using a simple test structure. The proposed method has a merit in the easiness of fabricating the test structure. Moreover, the electric fields inside the test structure are very similar to those inside the actual transistor, so that we can extract the information of bulk traps which directly affect the current collapse behaviors of AlGaN/GaN HEFTs. By applying the proposed method to the GaN buffer structures with various unintentionally doped GaN channel thicknesses, we conclude that the incorporated carbon into the GaN back barrier layer is the dominant origin of the bulk trap which affects the current collapse behaviors of AlGaN/GaN HEFTs.