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Analysis of Contributing Factor for Cation Ratio to Calcium in Nutrient Solution on the Incidence of Blossom-end Rot in Sweet Pepper 'RZ208' Grown in Hydroponics (파프리카 배꼽썩음과 발생에 미치는 배양액 내 칼슘에 대한 양이온 기여인자 분석)

  • Lee, Hye-Jin;Oh, Jeong-Sim;Choi, Ki-Young;Lee, Yong-Beom;Bae, Jong-Hyang;Rhee, Han-Cheol;Kim, Dong-Eok
    • Horticultural Science & Technology
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    • v.30 no.1
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    • pp.27-33
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    • 2012
  • This study aimed to investigate the responses of plant growth and blossom-end rot (BER) incidence to calcium (Ca) and its three kinds of antagonistic cations (K, Mg, and $NH_4$-N) with various ratios in nutrient solution for sweet paper (Capsicum annuum L. 'RZ208'). Both Ca to each cation and Ca to a series of cation combinations, such as potassium (K), ammonium nitrate ($NH_4$), or magnesium (Mg) were more influential to the fruit growth and quality than plant growth. Especially, the BER incidence was significantly influenced by the ratio treatments. For examples, when Ca:(K + Mg) or Ca:(K + Mg + $NH_4$) ratio was 1:2 the highest incident rate of BER about 70.3 or 86.3% was observed, lowering the marketable yield to 19 or 13.7% of the total yield, respectively. The correlation coefficiencies (= r) to relationships between the BER and K as well as BER and $NH_4$ were 0.82 (P < 0.05) and 0.65 (P < 0.05), respectively. Combination only with the Mg element was not correlated with the BER incidence. However, when both of the K and Mg concentrations were 0.65 (P < 0.05). The highest correlation coefficiency, 0.92 (P < 0.05), was found to a relationship between the BER and the tree elemental combination.

Relationships between Community Unit and Environment Factor in Forest Vegetation of Mt. Dutasan, Pyeongchang-gun (평창 두타산 산림식생의 군집유형과 입지환경요인의 상관관계)

  • Lee, Jeong Eun;Shin, Jae Kwon;Kim, Dong Gap;Yun, Chung Weon
    • Journal of Korean Society of Forest Science
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    • v.106 no.3
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    • pp.275-287
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    • 2017
  • The purpose of this study was to analyze forest vegetation type classification and relationships between the type and environment factor in Mt. Dutasan. Data were collected by total of forty six plots using Z-M phytosociological method from June to October, 2016, and analyzed by vegetation classification, canopy layer structure and relationships between vegetation unit and environment factor using coincidence methods. As a result of vegetation type classification, Quercus mongolica community group was classified at a top level of vegetation hierarchy that was classified into Rhododendron schlippenbachii community and Betula costata community. R. schlippenbachii community was divided into Lychnis cognata group and R. schlippenbachii typical group. L. cognata group was subdivided into Veratrum oxysepalum subgroup and L. cognata typical subgroup. B. costata community was divided into Fraxinus mandshurica group and Betula schmidtii group. F. mandshurica group was subdivided into Weigela subsessilis subgroup and Cimicifuga heracleifolia subgroup. Therefore the forest vegetation was composed of six vegetation units with two kinds of bisected species groups and fourteen species groups. As the result of an analysis of canopy layer structure, there were two kinds of structures with monotonous structures V. oxysepalum subgroup (vegetation units 1), L. cognata typical subgroup (vegetation units 2), W. subsessilis subgroup (vegetation units 4) and complicated structures R. schlippenbachii typical group (vegetation units 3), C. heracleifolia subgroup (vegetation units 5), Betula schmidtii group (vegetation units 6). The vertical layer structure of vegetation unit 5 was the most developed and vegetation unit 6 had the lowest coverage of herb layer. According to the correlation between vegetation unit and environmental factor, R. schlippenbachii community (vegetation units 1~3) and B. costata community (vegetation units 4~6) were classified based on 1,100 m of altitude, middle slope, twenty of slope degree, twenty percents of bare rock and thirty centimeters of DBH in tree layer. R. schlippenbachii community (vegetation units 1~3) showed positive correlation with altitude, topography and B. costata community (vegetation units 4~6) showed negative correlation tendency with them.

Ecological Studies on the Burned Forest -On the Productivity System of the Burned Forest- (산화적지(山火跡地)의 생태학적(生態學的) 연구(硏究) -산화후(山火後) 임지(林地)의 생산구조(生産構造)에 대(對)하여-)

  • Kim, Ok Kyung;Chong, Hyon Pae
    • Journal of Korean Society of Forest Science
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    • v.12 no.1
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    • pp.45-54
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    • 1971
  • Ecological studies on the effect of an accidental fire on the composition of the post-fire vegetation in relation to the productivity system were made at the burned site on Mt. Samak located at Duckduwon-Ri, Sumyun, Chun Sung-Kun, Kangwon-Do, the same plots used in the previous study carried out in 1967. The result are summarized as follows. 1. In the productivity system, the standing crop measured was as follows; Carex Lanceolata var. Nana, Miscanthus purpurscens etc. were contained in the herbs and their individual number was larger than that of the woody plants. (Table 1). In the woody plants, Quercus Acutissima was the most abundant, showing larger number of tree than Quercus dentata. The S.D.R. value of the family Poaceae was the highest among the herbs and in the test plots, it was 4 times larger in number than in the controlled plots. (Table 3, Fig. 4, 5). 2. In the unburned sities, 5 dominant species were selected and by calculating their S.D.R., it was shown that woody plants, S.D.R. is 4.43 while it is 11.52 with herbs. (Table 4, Fig. 6). 3. When making comparisons with the standing crop on the higher around, it was found that the test plots had 522.45 gm more than the controlled and 1470. 53gm more than those on lower ground. These results were considered to indicate that high temperature caused by fire resulted in the increase of germination rate of seeds as it was seen in the previous study and it further stimulate the growth of the perennial plants. (Table 6, 7) 4. In the number of species, the standing crop was increased in the order of Genus Miscanthus and Genus Carex. and in the woody plants Genus Lespedeza was increased in the standing crop. 5. It was found that in the rest plots, total summed height was greater by about 6000cm than that in the controlled plots. 6. In conclusion, the forest fire gave a great loss to tall trees and woody plants burning them together with unmatured seeds. In the succession of the 2nd year it was considered that the growth of the perennial plants had been stimulated on the barned sites.

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Isolation and Characterization of Starch-hydrolyzing Pseudoalteromonas sp. A-3 from the Coastal Sea Water of Daecheon, Republic of Korea (대한민국 대천 해안에서 분리한 전분 분해능을 갖는 Pseudoalteromonas sp. A-3 균주의 특징 및 동정)

  • Chi, Won-Jae;Park, Da-Yeon;Jeong, Sung-Cheol;Chang, Yong-Keun;Hong, Soon-Kwang
    • Microbiology and Biotechnology Letters
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    • v.39 no.4
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    • pp.317-323
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    • 2011
  • Strain A-3, an amylase-producing bacteria, was isolated from coastal seawater near Daecheon in the Republic of Korea. It was seen to possess a single polar flagella and grow well, on ASW-YP agar plates, at temperatures of between $20-37^{\circ}C$. However, it grew more slowly at the temperatures of $15^{\circ}C$ and $40^{\circ}C$. Similarly, it was observed to grow abundantly, in an Artificial Sea Water-Yeast extract-Peptone (ASW-YP) liquid medium, in a pH range of 6-9, but not grow at pHs of 4-5 and a pH of 10. Strain A-3 was noted as being close to Pseudoalteromonas phenolica O-$BC30^T$, Pseudoalteromonas luteoviolacea $NCIMB1893^T$, Pseudoalteromonas rubra $ATCC29570^T$, and Pseudoalteromonas byunsanensis $FR1199^T$, with 98.30%, 97.86%, 97.78%, and 97.25% similarities respectively, in its 16S rRNA sequence. A phylogenetic tree revealed that strain A-3 and P. phenolica O-$BC30^T$ belong to a clade. However, strain A-3 differed from P. phenolica O-$BC30^T$ in relation to a number of physiological characteristics. Strain A-3 exhibited no growth above 5% NaCl concentrations, no utilization of D-glucose, D-mannose, D-maltose, or D-melibose, and no lipase (C-14) activity. All of these properties strongly indicate that strain A-3 is distant from P. phenolica O-$BC30^T$ and thus led us to name it Pseudoalteromonas sp. A-3. Pseudoalteromonas sp. A-3 produces ${\alpha}$-amylase throughout growth. Maximal amylase activities of 144.48 U/mL and 149.20 U/mL were seen at pH 7.0 and $37^{\circ}C$, respectively. Pseudoalteromonas sp. A-3's high, stable production of ${\alpha}$-amylase in addition to its biochemical features, such as alkalitolerance, suggest that it is a good candidate for industrial applications.

Vegetation Distribution Near Abandoned Metalliferous Mines and Seed Germination Properties of Woody Plants by the Contaminated Soils (폐광산 주변의 목본 식생 현황 및 오염 토양에 대한 목본 종자의 발아 특성)

  • Seo, Kyung-Won;Kim, Rae-Hyun;Koo, Jin-Woo;Noh, Nam-Jin;Kyung, Ji-Hyun;Kim, Jeong-Gyu;Son, Yo-Whan
    • Korean Journal of Environmental Agriculture
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    • v.25 no.1
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    • pp.47-57
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    • 2006
  • This study was carried out to select the Eco-tree for successful phytoremediation of abandoned metalliferous mines. We examined vegetation and heavy metal concentrations of woody plants in abandoned mining areas, and also conducted seed germination and seedling growth experiment on contaminated soils from Gahak and Geumjeong mines. Pinus densiflora, Robinia pseudoacacia, Lespedeza bicolor and Alnus japonica showed high frequency in the survey areas and had high heavy metal concentrations compared to other species. Heavy metal concentrations were higher in roots than in leaves and stems. The seed germination rate was in the order of P. densiflora, L. bicolor, R. pseudoacacia, and Alnus japonica from the incubactor and greenhouse experiment. In the incubator experiment germination rate was highest in the control soil for P. densiflora and A. japonica. Germination rate of P. densiflora was highest on the 100% contaminated soil for Gahak mine while germination rate decreased with increased percentage of contaminated soil for Geumjeong mine. In the greenhouse experiment germination rate was lowest on the 40% contaminated soil for Gahak mine while germination rate was lowest on the 20% contaminated soil for Geumjeong mine and increased with increased percentage of contaminated soil. Shoot growth was highest for L. bicolor while root growth was highest for R. pseudoacacia except for 20% contaminated soil in Geumjeong mine.

Effect of Amount of Oil Cake Applications on Mineral Nutrient Partitioning of Black Chokeberry (유박시용량에 따른 유기 블랙초크베리의 수체 내 무기성분 분배에 미치는 영향)

  • Choi, Hyun-Sug;Jung, Seok-Kyu
    • Journal of the Korea Organic Resources Recycling Association
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    • v.28 no.1
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    • pp.5-14
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    • 2020
  • The study was initiated to reduce production cost and environmental pollution with the evaluation of nutrient requirement of 'Nero' black chokeberry (Aronia melanocarpa) and optimum amount of oil cake application. 100% of a recommended amount (RA) of oil cake was designated as a 100-RA, with 0-RA, 25-RA, 50-RA, and 75-RA for 0%, 25%, 50%, and 75% RA, respectively. The oil cake was scattered around the black chokeberry at every year for two years from 2018 to 2019, with investigation conducted for the second year. Soil mineral nutrient concentrations were not significantly different among the treatments. Dry weight (DW) of root and leaves was low for 0-RA-treated black chokeberry, with no significant difference observed for the all treatments for the DW of stems. 75-RA increased the fruit DW of 615 g and yield efficiency of 45.3%. Top:root ratio was the highest of 4.7 for 75-RA. Increased amount of oil cake application expanded the tree volume. Tree volume had a strong positive relationship with the root DW (r2=0.977). Mineral nutrient uptake in the root was the highest on the 25-RA-treated black chokeberry, except for Fe uptake. Mineral nutrient uptake in the leaves were similar to all the black chokeberries, except for T-N and Fe uptake. 75-RA increased mineral nutrient uptake in the fruit, except for Cu, in particular, 7.45 g in fruit N, which was the highest level compared to those of the other organs. T-N and P uptake were evenly distributed in the leaves, stems, and fruit, with high K uptake for leaves and fruit. 75-RA maximized to 17.2 g of the total nutrient uptake in a black chokeberry, with 4.9 g for the 0-RA. All mineral nutrient uptake were overall higher on the black chokeberry treated with 50-RA, 75-RA, and 100-RA compared to those of 0-RA and 25-RA.

Identification of DNA Methylation Markers for NSCLC Using Hpall-Mspl Methylation Microarray (Hpall-Mspl Methylation Microarray를 이용한 비소세포폐암의 DNA Methylation Marker 발굴)

  • Kwon, Mi Hye;Lee, Go Eun;Kwon, Sun Jung;Choi, Eugene;Na, Moon Jun;Cho, Hyun Min;Kim, Young Jin;Sul, Hye Jung;Cho, Young Jun;Son, Ji Woong
    • Tuberculosis and Respiratory Diseases
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    • v.65 no.6
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    • pp.495-503
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    • 2008
  • Background: Epigenetic alterations in certain genes are now known as at least important as genetic mutation in pathogenesis of cancer. Especially abnormal hypermethylation in or near promoter region of tumor suppressor genes (TSGs) are known to result in gene silencing and loss of gene function eventually. The authors tried to search for new lung cancer-specific TSGs which have CpG islands and HpaII sites, and are thought to be involved in carcinogenesis by epigenetic mechanism. Methods: Tumor tissue and corresponding adjacent normal tissue were obtained from 10 patients who diagnosed with non small cell lung cancer (NSCLC) and underwent surgery in Konyang university hospital in 2005. Methylation profiles of promoter region of 21 genes in tumor tissue & non-tumor tissue were examined with HpaII-MspI methylation microarray (Methyl-Scan DNA chip$^{(R)}$, Genomic tree, Inc, South Korea). The rates of hypermethylation were compared in tumor and non-tumor group, and as a normal control, we obtained lung tissue from two young patients with pneumothorax during bullectomies, methylation profiles were examined in the same way. Results: Among the 21 genes, 10 genes were commonly methylated in tumor, non-tumor, and control group. The 6 genes of APC, AR, RAR-b, HTR1B, EPHA3, and CFTR, among the rest of 11 genes were not methylated in control, and more frequently hypermethylated in tumor tissue than non-tumor tissue. Conclusion: In the present study, HTR1B, EPHA3, and CFTR are suggested as possible novel TSGs of NSCLC by epigenetic mechanism.

Ecophysiological Studies on the Water Relations of Economic Tree Species - Temporal Changes of Stomatal Responses to Soil Moisture Regimes and Exogenous Abscisic Acid in Oaks and Ash - (주요 경제 수종의 수분 특성에 관한 생리생태학적 연구 - 토양수분 조건 및 ABA 처리에 따른 참나무류와 물푸레나무 기공의 시계열적인 변화 반응 -)

  • Kwon, Ki Won;Lee, Jeoung Ho
    • Journal of Korean Society of Forest Science
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    • v.83 no.3
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    • pp.410-423
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    • 1994
  • Seasonal and diurnal changes of stomatal diffusive resistance(S.D.R.) and transpiration rate(T.R.) were investigated for determining the ecophysiological water relations of economic tree species subjected to chronic water stress or exogenous abscisic acid treatment. Four species of oaks including Quercus serrata, Q. mongolica. Q. acutissima, and Q. variabilis were used as the experimental materials and also Fraxinus rhynchophylla was studied together with oaks. Stomatal diffusive resistances were repeatedly measured on the containerized 1-0 year seedlings subjected to two kinds of soil moisture regime (wet and dry) in June, August, and September by LI-1600 Steady State Porometer of LI-Cor, Inc.. Exogenous abscisic acid (ABA) solutions of 0.5 mM and 0.05 mM in July and August, respectively, were absorbed into shoots cut from the containerized seedlings for determining their effects on stomatal behavior and transpiration. Most of measurements in stomatal diffusive resistance maintained about 5 s/cm in the morning after sunrise despite of different treatments. But the values fluctuated frequently to high level above 20 s/cm through the afternoon until sunset in the seedlings subjected to dry soil moisture regime. Despite of various treatments and environmental conditions, stomatal diffusive resistances of Q. variabilis were more stable than those of Q. serrata or Q. acutissima. Their values of F. rhynchophylla changed more irregularly in comparison with those of oak species. Exogenous abscisic acid absorbed into shoots cut from seedlings increased stomatal diffusive resistance obviously in most of the species studied. The stomatal responses to abscisic acid treatment were more sensitive in July especially in Q. serrata than in Q. variabilis and Q. acutissima. But the effects of ABA treatment were presented more remarkably in Q. acutissima in August. The responses to abscisic acid were not certain in F. rhynchophylla because of their various fluctuation patterns.

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Studies on the Host Plant, Bionomics, and Damage of Bamboo Leaf Rollers in Chonnam Province Area (대나무의 잎말이나방류(類)에 대한 기주(寄主), 생태(生態) 및 피해(被害)에 관한 연구(硏究))

  • Kim, Kyu-Chin;Lee, Tae-Sik
    • Korean journal of applied entomology
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    • v.25 no.2 s.67
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    • pp.85-92
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    • 1986
  • This study was conducted to investigate host plant, bionomics, and damage on Bamboo Leaf Rollers in Chonnam area. Species of the leaf rollers attacking the bamboo trees in Chonnam area were Microstega jessica (Butler) and Sinibotys evenoralis (Walker). Among these species, M. jessica (B.) and S. evenoralis (W.) were observed in Damyang but S. evenoralis (W.) alone was found in Naju and Hwasoon. Among the 4 host plant species observed, Phyllostachys nigra var. henonis S., and P. nigra M. were first recorded as host plants of M. jessica (B.), and P. nigra var. henonis (S.) as host plant of S. evenoralis (W.). The occurrence of the M. jessica (B). was once a year with the peak about late May, whereas the occurrence of S. evenoralis (W.) was 2 times a year and the peaks were early June and mid-July, respectively. The average periods of each stage of M. jessica (B.) were 8 days for egg, 293 days for larva, 16 days for pupa, and the longevity of the adult was 12 days for females and 9 days for males. The average periods of each stage of S. evenoralis (W.) were in 1st generation, 11 days for egg, 28 days for larva, 12 days for pupa, and the longevity of the adults was 18 days for females and 15 days for males; and 2nd generation was 7 days for egg, 261 days for larva, 20 days for pupa, and the longevity of the adults was 11 days for females and 10 days for males. The time of most adult emergence of M. jessica (B.) was from 6 p.m. to 4 a.m. and the peak was 8 p.m. to 10 p.m. M. jessica (B,) overwintered as 6th instals in Bamboo shoot sheath but S. evenoralis (W.) overwintered as $1st{\sim}2nd$ instals on the tree leaves of Bamboo. The percentage of damaged leaves with different heights of Bamboo trees were 67.1% at upper portion, 19.0% at middle portion, 14.0% at lower portion. The percentage of damaged loaves with different Bamboo species was 57.0% on P. nigra var. henonis S., 36.5% on P. bambusoides S. et Z., 14.5% on P. pubescens M.. It was showed highly significant negative correlation between percentage of damaged bamboo leaves an tree growth stages.($r=-0.739^{**}$)

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Floristic Composition, Structure, and Dynamics of Koelreuteria paniculata Communities in Daegu City (대구시 자생 모감주나무군락의 종조성, 구조 및 동태)

  • Kim, Jun-Soo;Cho, Joon-Hee;Kim, Hak-Yun;Cho, Hyun-Je
    • Journal of Korean Society of Forest Science
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    • v.108 no.2
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    • pp.168-176
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    • 2019
  • To understand the floristic composition, vegetation structure, and population dynamics of Koelreuteria paniculata communities, which are native to Daegu, South Korea, a field survey was conducted in July 2018 using phytosociological and complete enumeration methods. Based on information on vegetation and trees of >5 cm diameter at breast height collected during the field survey, we classified the community types by species composition and analyzed their vegetation strata, relative importance value (MIV), life forms, species diversity, and population structure. The community was divided into the following three types: Ulmus parvifolia-Vitex negundo var. incisa subcommunity, Securinega suffruticosa subcommunity, and Clematis apiifolia community. The vegetation unit system was organized into one community group, two communities, and two subcommunities. Vegetation coverage of the tree layer was >85%, while the herbaceous layer was <10%. MIV of K. paniculata appeared to be extremely high within all vegetation strata, with 94.3 of the tree layer, 81.6 of the subtree layer, 75.5 of the shrub layer, and 60.0 of the herbaceous layer. The species diversity (H') was significantly different among the community types, and the C. apiifolia community (2.062) was approximately four times higher than the S. suffruticosa subcommunity (0.547). The overall representative life form types were "$MM-R_5-D_4{\cdot}D_2-e$,", but there were some differences in the disseminule form among the community types. The population structure of K. paniculata showed the reverse J-shaped distribution with a high density of young individuals and low density of larger individuals among all three community types, and because no plant species within the lower vegetation could replace K. paniculata, it was considered to be a sustainable population.