• Journal of Food Hygiene and Safety
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• v.18 no.3
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• pp.101-106
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• 2003

The aim of this study is to develop a label-free immunosensor for microbial detection and to evaluate its applicability to Pseudomonas aeruginosa detection in various food samples. The antibodies used were a polyclonal antiserum from rabbit (polyvalent type) and a monoclonal antibody raised against the flagella of P. aeruginosa. Antibody immobilization was done by a thiolated antibody chemisorption onto one gold electrode of a piezoelectric quartz crystal with a thiol-cleavable, heterobifunctional cross-linker, sulfosuccinimidyl 6-[3-(2-pyridyldithio)propionamido]hexanoate. To the Stomacher-treated samples from various raw and processed foods under cold storage, comprising sirloin, cod and pettitoes, spiking and enrichment culture were done to prepare the model samples, followed by the measurements of the frequency shifts after sample injections. The frequency shifts obtained by the sample matrices themselves were in the range of 52~89 Hz. The injections of the spiked samples caused the frequency shifts of 108~200 Hz, whereas the enriched samples decreased the steady-state resonant frequencies by 162~222 Hz. All sample measurements including baseline stabilization, sample injection and acquisition of the steady-state response were accomplished within 30 min.

• Journal of the Mineralogical Society of Korea
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• v.21 no.4
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• pp.383-395
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• 2008

To enhance the grade and recovery rate of the gold/silver ores which yield at Philippine Mankayan mine, we studied the characteristics which are the geologic and mineralogical features of gold and silver ore, the liberation by crushing and grinding, the separation by sieving and shaking table. Gold/silver ore is composed of the sulfide minerals like pyrite, sphalerite, galena; and the gangue minerals which is quartz, clay. Gold/silver element are mainly contained in a sulfide minerals like pyrite, sphalerite and galena. To increase the liberation rate of sulfide minerals containing gold/silver element, the gold/silver ore has to be grounded under $100{\mu}m$ very finely because the crystal size of sulfide minerals is distributed from $1{\mu}m$ to $100{\mu}m$. The liberation rate of gold/silver ore increases to 92% when the particle size ($d_{90}$) of ore is grounded below $100{\mu}m$ by jaw crusher $\to$ cone crusher $\to$ rod mill by steps. The grade and recovery of sulfide minerals could not be enhanced by sieving separation because those crystal size is distributed homogeneously below $100{\mu}m$. But, when we separated the sieved ore using shaking table, the gold and silver grade increased to 40 ppm and 140 ppm, respectively. Then the recovery rate of gold reach almost 100% but that of silver is no more that 50%.

• The Journal of Engineering Research
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• v.4 no.1
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• pp.69-85
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• 2002

The chemical composition of bottle glass is consisted of Na2O-CaO-SiO2. However the cullet is mornally used in order to decrease the melting tsmperature. This induce the productivity of bottle and decreases the cost. The addition of plate glass decreases the Al2O3 content and in fluence the stone phenomenon and devitification in botle glass. Tus the Feldspar is added in order to increase the Al2O3 content when plate cullet was added in melting. The Tridymite crystal was observed over 7.5% Al2O3 contents, which shown as white crystal in appearance. It is Supposed that the Wollastonite Would be occurred in more over 7.5% Al2O3. This fad id well consised With the Litertctures.

• Proceedings of the Korean Society of Laser Processing Conference
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• 1998.11a
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• pp.0-0
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• 1998

-Part I- As market needs become more various, the production of smaller quantities of a wider variety of products becomes increasingly important. In addition, in order to meet demands for more efficient production, long-term unmanned factory operation is prevailing at a remarkable pace. Within this context, laser machines are gaining increasing popularity for use in applications such as cutting and welding metallic and ceramic materials. FANUC supplies four models of $CO_2$ laser oscillators with laser power ranging from 1.5㎾ to 6㎾ on an OEM basis to machine tool builders. However, FANUC has been requested to produce laser oscillators that allow more compact and lower-cost laser machines to be built. To meet such demands, FANUC has developed six models of Slab type YAG laser oscillators with output power ranging from 150W to 2㎾. These oscillators are designed mainly fur cutting and welding sheet metals. The oscillator has an exceptionally superior laser beam quality compared to conventional YAG laser oscillators, thus providing significantly improved machining capability. In addition, the laser beam of the oscillator can be efficiently transmitted through quartz optical fibers, enabling laser machines to be simplified and made more compact. This paper introduces the features of FANUC’s developed Slab type YAG laser oscillators and their applications. - Part II - All-solid-state lasers employing laser diodes (LD) as a source of pumping solid-state laser feature high efficiency, compactness, and high reliability. Thus, they are expected to provide a new generation of processing tools in various fields, especially in automobile and aircraft industries where great hopes are being placed on laser welding technology for steel plates and aluminum materials for which a significant growth in demand is expected. Also, in power plants, it is hoped that reliability and safety will be improved by using the laser welding technology. As in the above, the advent of high-power all-solid-state lasers may not only bring a great technological innovation to existing industry, but also create new industry. This is the background for this project, which has set its sights on the development of high-power, all-solid-state lasers with an average output of over 10㎾, an oscillation efficiency of over 20%, and a laser head volume of below 0.05㎥. FANUC Ltd. is responsible for the research and development of slab type lasers, and TOSHIBA Corp. far rod type lasers. By pumping slab type Nd: YAG crystal and by using quasi-continuous wave (QCW) type LD stacks, FANUC has already obtained an average output power of 1.7㎾, an optical conversion efficiency of 42%, and an electro-optical conversion efficiency of 16%. These conversion efficiencies are the best results the world has ever seen in the field of high-power all-solid-state lasers. TOSHIBA Corp. has also obtained an output power of 1.2㎾, an optical conversion efficiency of 30％, and an electro-optical conversion efficiency of 12%, by pumping the rod type Nd: YAG crystal by continuous wave (CW) type LD stacks. The laser power achieved by TOSHIBA Corp. is also a new world record in the field of rod type all-solid-state lasers. This report provides details of the above results and some information on future development plans.

• Journal of the Korean Institute of Telematics and Electronics
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• v.11 no.6
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• pp.44-49
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• 1974

reactance modulator composed of multi-stage phase modulator utilizing VVC diodes as variable reactance elements and an oscillartor-modulator, utilizing a VVC diode as a tuning element, coupled to a crystal resonator through an artificial λ/4 network are introduced and their characteristics as FM modulator are compared mutually from the practical view points. especially, to get high modulation sensitivity of reactance modulators using VVC diodes, making a multi-stage modulation distortion characteristics of multi-stage modulator was necessary. The harmonic moj\dulationdistorion characteristics of multi-stage reactance modulator is analized in detall. Multi-stage reachance modulator is preferable to maintain sufficiently stable carrier frequency over the wide range of temperature and a mobile-transceiver was made through this method. On the other hand, FM-Quartz oscillator using a VVc diode is suitavle for handy-talkies of good quality were made through this method.

• Journal of the Korean Electrochemical Society
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• v.6 no.2
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• pp.130-133
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• 2003

3,4-dihydroxybenzoic acid(3,4-DHBA) was electropolymerized on glassy carbon electrode to give the GC/p-3,4-DHBA type electrode which was modified with dopamine by the help of 1-(3-dimethylaminopropyl)-3-ethyl carbodiimide hydrochloride(EDC) acting as a coupling agent. The carboxylic sites on the polymeric surface of p-3,4-DHBA and mine group at the dopamine gave a QCA(Au)/p-3,4-DHBA-dopamine type of modified electrodes. The o-quinone moieties at the electrode surface exhibited high selectivity to titanium ions in solution. The redox process of the electrode is hydroquinone : quinone +$2H^+2e^-$, which had two strong and two weak pairs of peaks at CV. The modified electrode can deposit Ti(IV) ions as much as $4.13\times10^{-5}gcm^{-2}$. The calibration curve of the electrodes, log of the surface coverage-normalized redox response vs log[Ti], exhibited an excellent correlation$(r{\geq}0.997)$ for titanium concentrations ranging from $5.25\times10^{-4}\;to\;5.25\tiems10^{-8}M.$.

• Journal of Sensor Science and Technology
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• v.3 no.2
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• pp.33-39
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• 1994

A quartz crystal microbalance (QCM) sensor system for the detection of odorants, especially environmental pollutant, has been constructed by depositing various phospholipids, activated carbon and lead compound pigment onto the surface of the QCM. The characteristics of a QCM operating at 9 MHz deposited with phosphatidyl- choline were analysed. An explanation is given for different odorant affinities based on the monolayer properties of phospholipids. The identification of odorants is discussed in terms of a comparison of their normalized resonant frequency shift patterns and relative response intensities calculated from the response areas. Applying the lead compound pigment coated QCM, it was possible to detect sulfur compound specifically. Using a number of different lipid-coated QCMs, odorants could be identified by comparing the response patterns.

• Journal of fish pathology
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• v.20 no.3
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• pp.307-313
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• 2007

In this study, a QCM biosensor was made to detect Edwardsiella tarda (E. tarda) using a specific antibody. A 9 MHz AT-cut piezoelectric wafer layered with two gold electrodes of 5mm diameter had a reproducibility of 0.1 Hz in frequency response and was used as the transducer of the QCM biosensor. Self assembled layer (SAM) was conformed on a quartz crystal by treating with 3-mer-captopropionic acid (MPA) and activated with N-ethyl-N'-(3-dimethyl-aminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS). The resulting NHS group was further converted to hydrazide by the reaction with hydrazine. Aldehyde group was introduced into the carbohydrate moiety of anti-E. tarda antibody by the reaction with periodic acid and was used to immobilise the antibody through the reaction with hydrazide group on the electrode surface. A baseline was established in the presence of phosphate-buffered saline (PBS) and a resonant frequency (F1) was measured. Sample was added to the sensor surface and second resonant frequency (F2) was measured after unbound substances were washed out with PBS several times. Finally, the frequency shift (ΔF) representing the mass change was calculated by subtracting F2 from F1. After adding the oxidized anti-E. tarda antibody to the electrode surface containing hydrazide group, frequency shift of 288.811.4 Hz (mean S.E) was observed, thus proving that considerable amount of antibody was immobilized. In the immunoassay test, the frequency shift of 1877.75 Hz, 580.67 Hz, 221.39 Hz, 7.671.83 Hz (mean S.E) were observed at doses of 1000, 500, 100, 50 g of bacterial cells, respectively. It was also demonstrated that the prepared sensor chip was stable enough to withstand repeated surface regeneration with 0.2 M Tris-glycine and 1 % DMSO, pH 2.3 more than ten times.

• Journal of Surface Science and Engineering
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• v.43 no.6
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• pp.297-303
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• 2010

The effect of solution acidity and organic additives, polyethylene glycol (PEG), on the trivalent chromium electroplating was systematically investigated in the view point of electroreduction of trivalent chromium ions and solution stability. It was found that solution acidity controlled at pH 2.5 showed the widest current range for bright electrodeposits in the presence of PEG additives, which reduced the local current intensification at high current densities. Through complex interaction between PEG additives and hydrogen ion, that is, solution acidity, electrode potential was moved in the negative direction in the bulk solution, while it shifted in the positive when electric potential was scanned. In conjunction with electrochemical quartz crystal microbalance (EQCM), it was found that PEG additives had a role in promoting the electron transfer to trivalent chromium ion complexes in bulk solution and their adsorption at the electrode surface as well as interfering with hydrogen ion reduction process below pH 2.5. The PEG additives developed the nodular morphology during electroreduction of trivalent chromium ions with the increase of solution acidity and enhanced its current efficiency by maintaining the consumption of complexant, formic acid, at low speed.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2004.11a
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• pp.452-455
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• 2004

In this paper, investigations of the SAMs(self-assembled monolayers) of a thiol-fuctionalized viologen derivatives, $V_8SH$ and $SH_8V_8SH$, where, V is N,N'-dialkylbipyridinium (i.e. a viologen group), have been carried out by elucidate voltammetry date. The redox reactions are highly reversible and can be cycled many times without significant side reaction, which has been known as a nano-gram order mass detector through resonant frequency change self-assembly process of the viologen has been investigated with $QCM({\Delta}F)$. The assembling process of the $V_8SH$ and $SH_8V_8SH$ monolayers can be finished completely in about 1 hour. The measured frequency shift for $V_8SH$ and $SH_8V_8SH$ were about 351 and 172 Hz, respectively. From these values, we calculated that the mass adsorbed $V_8SH$ and $SH_8V_8SH$ were about 375 and 183 ng. We believe that this mass loss is caused by the simultaneous loss of the anions present within the monolayer for charge compensation of the viologen dications and some solvent.