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Detection of Pseudomonas aeruginosa with a Label-free Immunosensor from Various Cold Storage Foods  

Kim, Nam-Soo (Korea Food Research Institute)
Park, In-Seon (Korea Food Research Institute)
Kim, Dong-Kyung (Korea Food Research Institute)
Publication Information
Journal of Food Hygiene and Safety / v.18, no.3, 2003 , pp. 101-106 More about this Journal
Abstract
The aim of this study is to develop a label-free immunosensor for microbial detection and to evaluate its applicability to Pseudomonas aeruginosa detection in various food samples. The antibodies used were a polyclonal antiserum from rabbit (polyvalent type) and a monoclonal antibody raised against the flagella of P. aeruginosa. Antibody immobilization was done by a thiolated antibody chemisorption onto one gold electrode of a piezoelectric quartz crystal with a thiol-cleavable, heterobifunctional cross-linker, sulfosuccinimidyl 6-[3-(2-pyridyldithio)propionamido]hexanoate. To the Stomacher-treated samples from various raw and processed foods under cold storage, comprising sirloin, cod and pettitoes, spiking and enrichment culture were done to prepare the model samples, followed by the measurements of the frequency shifts after sample injections. The frequency shifts obtained by the sample matrices themselves were in the range of 52~89 Hz. The injections of the spiked samples caused the frequency shifts of 108~200 Hz, whereas the enriched samples decreased the steady-state resonant frequencies by 162~222 Hz. All sample measurements including baseline stabilization, sample injection and acquisition of the steady-state response were accomplished within 30 min.
Keywords
label-free immunosensor; thiolated antibody chemisorption; Pseudomonas aeruginosa detection; cold storage foods;
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