• Mass Spectrometry Letters
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• v.4 no.2
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• pp.25-29
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• 2013

Proteomics for biomarker validation needs high throughput instrumentation to analyze huge set of clinical samples for quantitative and reproducible analysis at a minimum time without manual experimental errors. Sample preparation, a vital step in proteomics plays a major role in identification and quantification of proteins from biological samples. Tryptic digestion a major check point in sample preparation for mass spectrometry based proteomics needs to be more accurate with rapid processing time. The present study focuses on establishing a high throughput automated online system for proteolytic digestion and desalting of proteins from biological samples quantitatively and qualitatively in a reproducible manner. The present study compares online protein digestion and desalting of BSA with conventional off-line (in-solution) method and validated for real time sample for reproducibility. Proteins were identified using SEQUEST data base search engine and the data were quantified using IDEALQ software. The present study shows that the online system capable of handling high throughput samples in 96 well formats carries out protein digestion and peptide desalting efficiently in a reproducible and quantitative manner. Label free quantification showed clear increase of peptide quantities with increase in concentration with much linearity compared to off line method. Hence we would like to suggest that inclusion of this online system in proteomic pipeline will be effective in quantification of proteins in comparative proteomics were the quantification is really very crucial.

• Journal of Korea Society of Industrial Information Systems
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• v.14 no.5
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• pp.281-288
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• 2009

The purpose of this paper is to provide the statistical models for landslide prediction through quantification and AHP methods. Quantification method is a statistical method of providing quantity to qualitative variables by analyzing the observed data. In this paper, we suggest the quantification process based on the results of cannonical correlation analysis. In contrast with the quantification method which is based on given data the AHP(Analytic Hierarchy Process) technique is a kind of method based on questionaire data which is usually taken from professionals. We analyze both the real data(provided from KIGAM) and questionaire data collected from professionals of various related area. We developed two kinds of evaluation table which provide the scores of land slide possibility and the logistic model providing the probability of occurring landslide. Finally we compare the performance and evaluate the stability of the suggested two models.

• Nuclear Engineering and Technology
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• v.54 no.3
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• pp.790-802
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• 2022

Large-scale reactor simulation often requires the use of Monte Carlo calculation techniques to estimate important reactor parameters. One drawback of these Monte Carlo calculation techniques is they inevitably result in some uncertainty in calculated quantities. The present study includes parametric uncertainty quantification (UQ) and sensitivity analysis (SA) on the Advanced Test Reactor Critical (ATRC) facility housed at Idaho National Laboratory (INL) and addresses some complications due to Monte Carlo uncertainty when performing these analyses. This approach for UQ/SA includes consideration of Monte Carlo code uncertainty in computed sensitivities, consideration of uncertainty from directly measured parameters and a comparison of results obtained from brute-force Monte Carlo UQ versus UQ obtained from a surrogate model. These methodologies are applied to the uncertainty and sensitivity of k_eff for two sets of uncertain parameters involving fuel plate geometry and fuel plate composition. Results indicate that the less computationally-expensive method for uncertainty quantification involving a linear surrogate model provides accurate estimations for k_eff uncertainty and the Monte Carlo uncertainty in calculated k_eff values can have a large effect on computed linear model parameters for parameters with low influence on k_eff.

• The Korean Journal of Applied Statistics
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• v.14 no.2
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• pp.305-320
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• 2001

다차원선호분석(mutidimensional preference analysis)은 여러 상품들에 대한 개인(또는 그룹)의 선호도를 알아보기 위한 분석방법으로 결과는 보통 2차원 그림으로 제공된다. 본 연구에서는 의미있는 두 가지 최적척도 기준을 제안하고 이와 연관된 행 및 열표시자를 유도하고 있으며, 아울러 사전지식을 반영하기 위해 부분수량화를 다차원선호분석에 도입하는 방법을 제시한다. 또한 본 연구에서 제시한 다차원분석기법들을 실제 인터넷 검색엔진에 대한 선호도 자료에 적용한다.

• Nuclear Medicine and Molecular Imaging
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• v.43 no.5
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• pp.451-458
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• 2009

Purpose: Optical molecular luminescence imaging is widely used for detection and imaging of bio-photons emitted by luminescent luciferase activation. The measured photons in this method provide the degree of molecular alteration or cell numbers with the advantage of high signal-to-noise ratio. To extract useful information from the measured results, the analysis based on a proper quantification method is necessary. In this research, we propose a quantification method presenting linear response of measured light signal to measurement time. Materials and Methods: We detected the luminescence signal by using lab-made optical imaging equipment of animal light imaging system (ALIS) and different two kinds of light sources. One is three bacterial light-emitting sources containing different number of bacteria. The other is three different non-bacterial light sources emitting very weak light. By using the concept of the candela and the flux, we could derive simplified linear quantification formula. After experimentally measuring light intensity, the data was processed with the proposed quantification function. Results: We could obtain linear response of photon counts to measurement time by applying the pre-determined quantification function. The ratio of the re-calculated photon counts and measurement time present a constant value although different light source was applied. Conclusion: The quantification function for linear response could be applicable to the standard quantification process. The proposed method could be used for the exact quantitative analysis in various light imaging equipments with presenting linear response behavior of constant light emitting sources to measurement time.

• Journal of Microbiology and Biotechnology
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• v.19 no.7
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• pp.658-665
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• 2009

Quality control QC for spot-uniformity is a critical point in fabricating an oligonucleotide array, and quantification of targets is very important in array analysis. We developed two new types of QC probes as a means of confirming the quality of the uniformity of attached probes and the quantification of targets. We compared the signal intensities and fluorescent images of the QC and target-specific probes of arrays containing only target-specific probes and those containing both QC and target-specific probes. In a comparison of quality control methods, it was found that the arrays containing QC probes could check spot-uniformity or spot defects during all processes of array fabrication, including after spotting, after washing, and after hybridization. In a comparison of quantification results, the array fabricated by the method using QC probes showed linear and regular results because it was possible to normalize variations in spot size and morphology and amount of attached probe. This method could avoid errors originating in probe concentration and spot morphology because it could be normalized by QC probes. There were significant differences in the signal intensities of all mixtures (P<0.05). This result indicates that the method using QC probes is more useful than the ordinary method for quantification of mixed target. In the quantification of mixed targets, this method could determine a range for mixed targets of various amounts. Our results suggest that methods using QC probes for array fabrication are very useful to the quality control of spots in the fabrication processes of quantitative oligonucleotide arrays.

• Proceedings of the Korean Institute of Building Construction Conference
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• 2017.11a
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• pp.195-196
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• 2017

The number of human accidents in the construction industry is increasing every year, and it constitute the highest percentage among industry. This means that activities performed to prevent safety accidents in the country are not efficient to reduce the rate of accidents in the construction industry. In order to solve this issue, research has been conducted from various perspectives. But, research regarding to quantification model of human accidents is insufficient. the objective of this study is to conduct a basic study on quantification model development of human accidents. To achieve the objective, first, Cause of accident is defined the through literature review. Second, a basic statistic analysis is conducted to determine the characteristics of the accident causes. Third, the analysis is conducted after dividing into four categories : accumulate rate, season, total construction cost, and location. In the future, this study can be used as a reference for developing the safety management checklist for safety management in construction site and development of prediction models of human accident.

• Mass Spectrometry Letters
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• v.5 no.2
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• pp.52-56
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• 2014

Glycated hemoglobin (HbA1c) is used as an index of mean glycemia over prolonged periods. This study describes an optimization of enzyme digestion conditions for quantification of non-glycated hemoglobin (HbA0) and HbA1c as diagnostic markers of diabetes mellitus. Both HbA0 and HbA1c were quantitatively determined followed by enzyme digestion using isotope dilution liquid chromatography-tandem mass spectrometry (ID-LC-MS/MS) with synthesized N-terminal hexapeptides as standards and synthesized isotope labeled hexapeptides as internal standards. Prior to quantification, each peptide was additionally quantified by amino acid composition analysis using ID-LC-MS/MS via acid hydrolysis. Each parameter was considered strictly as a means to improve digestion efficiency and repeatability. Digestion of hemoglobin was optimized when using 100 mM ammonium acetate (pH 4.2) and a Glu-C-to-HbA1c ratio of 1:50 at $37^{\circ}C$ for 20 h. Quantification was satisfactorily reproducible with a 2.6% relative standard deviation. These conditions were recommended for a primary reference method of HbA1c quantification and for the certification of HbA1c reference material.

• The Korean Journal of Applied Statistics
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• v.19 no.2
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• pp.331-338
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• 2006

Quantification method III is designed by C. Hayashi as visualizing technique for two-way cross-classified tables. Specially in Japan, its usefulness is timely proven in social and marketing surveys. In several instances, relatively large quantification scores are assigned to low-frequency categories. Thus, they lead to unreliable data interpretation. The aim of this study is to develop stable solution to overcome such traits of quantification method III. The solution is of shrinkage type induced by small perturbations and is applied to a multiple response data obtained in a Korean social survey.

• Science of Emotion and Sensibility
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• v.16 no.4
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• pp.503-508
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• 2013

This study aims to analyze using RQA(Recurrence Quantification Analysis) about difference of electroencephalogram between inattention and attention among nonlinear methods for school age children who need attention. The experiments were conducted by 21 healthy subjects(12 males and 9 females). Inattention state is 500msec before the beginning of the auditory stimuli, attention state is 500msec after the beginning of auditory stimuli. The results of RQA parameters are greater in attention state than inattention state. It showed a statistically difference(p < 0.05). According to two states, auditory evoked potentials are displayed RP and CRP in diagram form to confirm nonlinear characteristics and The brain dynamics in the attention is more complex than the inattention. It is feasible that the RQA can be useful for the analysis of complex brain dynamics associated during auditory attentional task.