• Development and Reproduction
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• v.14 no.4
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• pp.261-268
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• 2010

As in the O. mykiss electrophoretic profiles of RNA, the signals of each RNA sample from 9 individual tissues such as liver, muscle, brain, heart, pituitary gland, kidney, intestine, spleen and gill similar to positive control were obtained. The tissue distributions of the complimentary DNA (cDNA) of O. mykiss four genes were analyzed using quantitative real-time PCR with primer sets for tissue expression analysis. In this rainbow trout species, author obtained bands of various sizes, ranged from 700 bp to 1,400 bp. A dissociation curve was made at the end of each run to make sure that there was no non-specific amplification. Supplementarily, the Ct of each DNA was compared. The Ct values of vinculin with rainbow trout tissues were determined in a manner similar to those for agouti-related protein (AgRP) and melanocortin receptors (MC4R I and MC4R II). Further, obtained Cts for standard curve of each DNA were affected by specific product (vinculin, AgRP and MC4R II genes). After several experiments with four individual genes of rainbow trout, author estimated a variation ratio of the mean Ct value of the DNA extracted using the comparative CTt method was 37.27, and the standard deviation was 5.33. The correlation coefficient between the Ct values and the concentration of cDNA was -0.98, -0.99, -0.91 and -0.86, respectively (vinculin, AgRP, MC4R I and MC4R II genes). Since this correlation showed high linearity, the straight line obtained was used as a standard for the O. mykiss tissues reared in aquarium. A PCR efficiency of 100% is ideally achieved when the slopes are close to the theoretical value of -3.31. According to quantification method, the results of quantification are strongly affected by the DNA fragmentation. The size of most DNA fragments obtained from various tissues of rainbow trout used in the experiment was approximately 100 bp. According to the four slopes, an efficiency of nearly 100% was estimated for four genes detection methods. Additionally, further analysis with more individuals and primers will be required to fully establish optimization in rainbow trout.

• Proceedings of the Korea Society for Industrial Systems Conference
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• 2009.05a
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• pp.114-119
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• 2009

본 논문에서는 수량화 방법과 AHP(Analytic Hierarchy Process) 기법을 사용하여 산사태 발생에 대한 통계적 예측모형을 구축하는데 목적이 있다. 수량화(Quantification) 방법은 질적변수에 수량을 부여하는 통계적 방법으로, 기 조사된 자료에 기반하여 분석을 수행하는 방법이다. 본 논문에서는 서구의 다변량분석 기법인 정준상관분석의 결과를 토대로 수량화 과정을 구체적으로 제안한다. 데이터에 기반한 수량화 방법과는 달리 AHP(Analytic Hierarchy Process) 기법은 일종의 다기준 의사결정을 위해 사용되는 기법으로, 설문자료에 기반한 분석법이다. 실제자료에 대한 분석으로 산사태 발생여부를 측정한 자료(한국지질자원연구원 제공)와 전문가 설문을 통해 수집된 자료를 이용하였다. 이들 자료에 대해 수량화 분석과 AHP분석을 통해 산사태 발생여부를 예측할 수 있는 두 종류의 평가표와 함께 로지스틱 회귀를 통한 통계적 예측모형을 개발하였으며, 두 모형간의 성능비교와 안정성 평가를 수행하였다.

• The KSFM Journal of Fluid Machinery
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• v.10 no.4
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• pp.47-54
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• 2007

Simultaneous 3-D LDV measurements of the in-cylinder flows of three different engine setups were summarized for the quantification of the flow characteristics in each vertical or horizontal plane, and in entire cylinder volume. The ensemble averaged-velocity, tumble and swirl motions, and turbulent kinetic energy during the intake and compression strokes were examined from the measured velocity data (approximately 2,000 points for each engine setup). The better spatial resolution of the 3-D LDV allows measurements of the instantaneous flow structures, yielding more valuable information about the smaller flow structures and the cycle-to-cycle variation of these flow patterns. Tumble and swirl ratios, and turbulent kinetic energy were quantified as planar and volumetric quantities. The measurements and calculation results were animated for the visualization of the flow, and hence ease to analysis.

• Wind and Structures
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• v.13 no.2
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• pp.207-220
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• 2010

The 'chain reaction' effect of the interaction between wind pressure and windborne debris is likely to be a major cause of damage to residential buildings during severe wind events. The current paper (Part II) concerns the quantification of such pressure-debris interaction in an advanced vulnerability model that integrates the debris risk model developed in Part I and a component-based wind-pressure damage model. This vulnerability model may be applied to predict the cumulative wind damage during the passage of particular hurricanes, to estimate annual hurricane losses, or to conduct system reliability analysis for residential developments, with the effect of windborne debris fully considered.

• Reproductive and Developmental Biology
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• v.36 no.1
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• pp.55-64
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• 2012

Peroxiredoxin II (Prdx II; a typical 2-Cys Prdx) has been originally isolated from erythrocytes, and its structure and peroxidase activity have been adequately studied. Prdx II has been reported to protect a wide range of cellular environments as antioxidant enzyme, and its dysfunctions may be implicated in a variety of disease states associated with oxidative stress, including cancer and aging-associated pathologies. But, the precise mechanism is still obscure in various aspects of aging containing ovarian aging. Identification and relative quantification of the increased proteins affected by Prdx II deficiency may help identify novel signaling mechanisms that are important for oxidative stress-related diseases. To identify the increased proteins in Prdx $II^{-/-}$ mice, we performed RBC comparative proteome analysis in membrane fraction and cytosolic fractions by nano-UPLC-$MS^E$ shotgun proteomics. We found the increased 86 proteins in membrane (32 proteins) and cytosolic (54 proteins) fractions, and analyzed comparative expression pattern in healthy RBCs of Prdx $II^{+/+}$ mice, healthy RBCs of Prdx $II^{-/-}$ mice, and abnormal RBCs of Prdx $II^{-/-}$ mice. These proteins belonged to cellular functions related with RBC lifespan maintain, such as cellular morphology and assembly, cell-cell interaction, metabolism, and stress-induced signaling. Moreover, protein networks among the increased proteins were analyzed to associate with various diseases. Taken together, RBC proteome may provide clues to understand the clue about redox-imbalanced diseases.

• Spatial Information Research
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• v.20 no.3
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• pp.57-66
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• 2012

Gangwon-do has been suffering extensive landslide dam age, because its geography consists mainly of mountains. Analyzing the related factors is crucial for landslide prediction. We digitized the landslide and non-landslide spots on an aerial photo obtained right after a disaster in Inje, Gangwon-do. Three landslide factors-topographic, forest type, and soil factors-w ere statistically analyzed through GIS overlap analysis between topographic map, forest type map, and soil map. The analysis showed that landslides occurred mainly between the inclination of $20^{\circ}$ and $35^{\circ}$, and needleleaf tree area is more vulnerable to a landslide. About soil properties, an area with shallow effective soil depth and parent material of acidic rock has a greater chance of landslide.

• Journal of Forest and Environmental Science
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• v.14 no.1
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• pp.145-156
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• 1998

On the basis of data obtained from 5 forest roads(Backyang, Byongatae, Saorang, Bukyu and Dangrim forest road) collapsed under a heavy rainfall in Chunchon, Kangwondo, this study was carried out to predict the cutting slope failure of forest road by using Quantification theory(II). The results were summarized as follows; The cutting slope failure was chiefly occurred by correlated action of road structure, vegetation and topographical factors. The cutting slope failure predicted by partial correlation coefficients and range values was characterized by longer than 8m of cutting slope length, depper than 2.5m of soil depth, between $30^{\circ}$ and $50^{\circ}$ of original ground slope gradient, absence of vegetation coverage on cutting slope, and greater than $60^{\circ}$ of cutting slope gradient. And the rate of correct discrimination by analysis of cutting slope failure was 90.1%.

• Journal of Korean Society of Transportation
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• v.27 no.2
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• pp.117-131
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• 2009

When most drivers take to the freeway, they don't necessarily pay attention to the geometric design. They expect proper design by depending on their own senses and recognition. When they evaluate the features of traveling on the freeway, they can think differently than engineers. The design needs to predict the exact speed of the driver to satisfy the driver's expectation, safety, pleasure and so on. This study categorized the factors influencing the speed of six freeways considering geometric and operational features to make a prediction model of speed. The model used multiple regression with these factors and produced statically appropriate results. This study utilized the principle component analysis and the quantification II analysis based on the image data of the satisfaction of the traveling environment collected through individual interviews. As a result, this study found the factors of satisfaction in a traveling environment. It made a satisfaction model of the traveling environment on freeways considering the change of driver's actual recognition and societal recognition using structural equations and the quantification II theory. Through the model made in this study, This model can present not only qualitative factors like satisfaction of traveling environment on freeways, but also the quantitative elements like speed. What is important is the evaluation of features of traveling on freeways reflected in the recognition and traffic environment felt by drivers.

• Journal of Korean Society of Forest Science
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• v.88 no.2
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• pp.240-248
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• 1999

On the basis of data obtained from five forest roads collapsed due to a heavy rainfall of 1995 in Chunchon, Kangwon-do, this study was carried out to evaluate and predict the fill slope failure of forest roads with four factors of forest road structure and those of location condition by using Quantification theory(II). The results were summarized as follows ; In the structure factors of forest road, the fill slope failure was mainly occurred in longitudinal gradients less than $2^{\circ}$ or more than $4^{\circ}$, distance of surface-flow longer than 80m, fill slope length greater than 6m, and fill slope gradients steeper than $35^{\circ}$. In the factors of location condition, the failure was mainly occurred in ridge portion of road position, weathered rock and soft rock of constituent material, slope gradients in the range from $35^{\circ}$ to $45^{\circ}$, and concave and convex of longitudinal slope forms. The priority order for factors influencing on fill slope failure was ranked by fill slope length, constituent material, road position, and so on. And the rate of correct discrimination by analysis of fill slope failure was estimated at the high prediction of 86.5%.

• Proceedings of the PSK Conference
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• 2002.10a
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• pp.396.1-396.1
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• 2002

HPTLC(High Performance Thin Layer Choromatography) method was developed for rapid and precise analysis of Sildenafil and modified Sildenafils(Vardenafil. Homosildenafil, Tadanafil). Chromatographic conditions were Optimized for simultaneous analysis of them and each specific UV spectra were obtained. The calibration curve of Sildenafil and modified Sildenafils had a linearity in the range of 1.0 ~ 56.5 $\mu$/ml at 254nm. The Limit of Detection(LOD) and the limit of Quantification(LOQ) of Sildenafil and modified Sildenafils were 0.8$\mu$/ml and 1.0$\mu$g/ml. (omitted)