• Asian-Australasian Journal of Animal Sciences
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• 제25권6호
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• pp.895-902
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• 2012

The Korean native chickens (Woorimotdak$^{TM}$, KNC) and commercial broilers (Ross, CB) show obvious differences in meat flavor after cooking. To understand the contribution of protein and peptide for meat flavor, 2-dimensional (2-D) gel electrophoresis and matrix-assisted laser desorption-ionization time-of-flight (MALDI-TOF) mass spectrometry was performed. A total of 16 protein spots were differentially expressed in the breast and thigh meat between the two breeds. A total of seven protein spots were represented by different levels between KNC and CB for breast meat. Among them three protein spots (TU39149, TU40162 and TU39598) showed increases in their expressions in KNC while other four protein spots (BU40125, BU40119, BU40029 and BU39904) showed increases in CB. All nine protein spots that were represented by different levels between KNC and CB for thigh meat showed increases in their expression in KNC. Phosphoglucomutase 1 (PGM 1), myosin heavy chain (MyHC), heat shock protein B1 (HSP27), cytochrome c reductase (Enzyme Q), Glyoxylase 1, DNA methyltransferase 3B (DNA MTase 3) were identified as the main protein spots by MALDI-TOF mass spectrometry. These results can provide valuable basic information for understanding the molecular mechanism responsible for breed specific differences in meat quality, especially the meat flavour.

• 한국동물위생학회지
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• 제27권1호
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• pp.17-29
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• 2004

A multiresidual analysis was performed to determine 12 sulfonamides(sulfacetamide, sulfadiazine, sulfisomidine, sulfathiazole, sulfapyridine, sulfamerazine, sulfamethazine, sulfamonomethoxine, sulfisoxazole, sulfamethoxazole, sulfaquinoxaline, and sulfadimethoxine) in beef and pork simultaneously. The multiresidual analysis for the sulfonamides currently used was able to analyze 5 kinds of sulfonamides at the same time. The method of this 12 sulfonamides multiresidual analysis in this study was matrix solid-phase dispersion(MSPD) by high performance liquid chromatography (HPLC) and liquid chromatography mass spectrometry (LC/MS). The recovery rate of the materials was measured by MSPD method with 3 different extraction solvents; Dichloromethane, DCM: Ethylacetate(3:1), DCM:EA(9:1). Also, samples (84 beef and 205 pork samples) which were positive by EEC-4 plate test from 2001 to 2003 were tested to investigate the kinds of sulfonamides using HPLC. The results from the study were as follows; 1. The recovery rate of the materials was measured by MSPD method with 3 different extraction solvents; Dichloromethane, DCM:Ethylacetate(3:1), DCM:EA(9:1). The method of extraction solvent with DCM:ethyl acetate(9:1) was the most excellent(87.7∼99.3%) in separation and reappearance. 2. In the LC/MS analysis. of sulfonamides, signal to noise ratio was showed relatively high in the positive mode and special ion in the quality analysis was determined via [M+H]$\^$+/ and m/z 156. A spectrum of sulfonamides was showed from all 12 sulfonamides. 3. The samples positive by the EEC-4 plate, a screening test method, were categorized by sulfonamides through Charm II and confirmed the kinds of sulfonamides through HPLC. 1) Among 84 beef samples positive by EEC-4 plate, 20 samples were positive by Charm II and identified as 7 sulfamethazine, 9 sulfadimethoxine, 1 sulfamonomethoxine and 3 unknown status. 2) Among 205 pork samples positive by EEC-4 plate, 42 samples were positive by Charm II and identified as 19 sulfamethazine, 1 sulfadimethoxine, 4 sulfamonomethoxine and 5 unknown status.

• 한국산학기술학회논문지
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• 제20권1호
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• pp.304-312
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• 2019

4차 산업혁명 시대에 서비스기업에서도 각기 다른 다양한 차별화 정책을 내세우고 있다. 기존까지 단순하게 고객을 만족시켜 매출을 높이기위한 방법이 아닌 좀 더 원시안적 시각을 갖고 서비스를 제공하는 직원들의 마인드와 그들이 느끼는 환경에 대한 중요성이 점차 커지고 있다. 서비스기업은 궁극적으로 고객만족, 품질향상, 가치 있는 경영 등 긍정적인 경영성과를 나타내기 위하여 끊임없는 노력을 기울이고 있으며 다른 산업에 비하여 직원에 대한 높은 의존도를 가지고 있다. 따라서 고객들을 직접적으로 응대해야하는 서비스제공자인 직원의 마음가짐과 부여된 환경, 역량 등이 직 간접적으로 고객만족 및 성과에 큰 영향을 미칠 수 있다. 궁극적인 조직의 성과를 높이고 경쟁우위를 갖추기 위해서는 결국 서비스 제공자인 직원들에 대하여 개인역량 개발, 근무환경 지원 등 다양한 형태의 사회적 지지가 중요하다고 할 수 있다. 따라서 본 연구에서는 사회적 지지가 서비스종사원의 긍정심리자본, 창의성, 직무성과에 미치는 영향에 관한 연구에 대한 후속연구로 서비스프로세스 메트릭스 가운데 대량서비스영역인 도매점, 소매점, 학교, 은행 등에 종사하는 직원들을 대상으로 동료지지가 자기 효능감을 통하여 성과에 어떠한 영향을 주는지 살펴보았으며, 그 결과 총 3개의 가설 중 3개 모두 채택되었다. 동료와 전반적인 관계가 원활해지면 자기 효능감 또한 향상되고, 자기효능감이 높을수록 직무성과도 높게 나타나며, 동료와 업무적으로나 업무이외에 전반적으로 의지할 수 있다고 느끼는 경우 직무성과도 높게 나타나는 것을 알 수 있다. 본 연구는 대량서비스영역에 종사하는 직원들을 실증분석 대상으로 한 관련연구는 전무한 실정으로 지금까지 연구와는 좀 더 다른 관점으로 접근하여 서비스기업의 경쟁력을 높이기 위한 인적자원관리 측면에서의 전략을 좀 더 효율적이고 효과적으로 제시하여 관련 시사점을 제시하고자 한다.

• Animal Bioscience
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• 제34권12호
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• pp.2003-2011
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• 2021

Objective: The aim of this study was to evaluate the effect of marinating turkey meat with buttermilk and acid whey on the technological traits and microbiological quality of the product. Methods: Slices of turkey meat muscles were marinated for 12 hours in buttermilk (n = 30), acid whey (n = 30) and comparatively, in lemon juice (n = 30). The control group (n = 30) consisted of unmarinated slices of turkey breast muscles. Physical parameters (pH, water holding capacity, colour L^*a^*b^*, shear force, weight loss) were assessed and quantitative and qualitative microbiological evaluation of raw and roasted products was performed. The microbiological parameters were determined as the total viable counts of mesophilic aerobic bacteria, of the Enterobacteriaceae family, and Pseudomonas spp. Bacterial identification was performed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Results: Marinating turkey meat in buttermilk and whey compared to marinating in lemon juice and the control sample resulted in a higher (p<0.05) degree of yellow color saturation (b^*) and a reduction (p<0.05) in the number of mesophilic aerobic bacteria, Pseudomonas spp. and Enterobacteriaceae family as well as the number of identified mesophilic aerobic bacteria in both raw and roasted samples. The lowest (p<0.05) shear force values were found in products marinated in whey. Conclusion: The use of buttermilk and acid whey as a marinade for meat increases the microbiological safety of the product compared to marinating in lemon juice, while maintaining good technological features of the product.

• 대한환경공학회지
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• 제35권2호
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• pp.75-83
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• 2013

본 연구는 기존의 고상추출(SPE) 과정을 분석장비인 LC/MSD에 on-line으로 연결함으로써 cartridge의 건조를 막아 target 물질의 손실을 최대한 억제하였으며 11종의 PFCs에 대해 실제 matrix가 있는 낙동강 원수를 이용한 실험에서 $80.4{\pm}5.2%{\sim}109.5{\pm}1.4%$ 범위의 높은 회수율을 구할 수 있었다. 낙동강에서의 PFCs 분포를 조사한 결과 하수처리장 방류수의 영향을 받는 지역(진천천과 금호강 하류)은 고농도의 PFCs가 검출되었으며, 낙동강 하류의 매리원수에서는 검출농도가 급격히 감소하여 PFOA만 8.0 ng/L의 농도로 검출되었다. 외국의 PFCs 검출현황을 보고한 연구결과들과 비교해 볼 때 낙동강 지류에서는 비교적 높은 농도로 PFCs가 검출되어 안심할 수 있는 수준은 아니었으며, 낙동강 본류의 경우도 여러 지역의 상수원으로 이용되기 때문에 주기적인 모니터링이 필요한 것으로 나타났다.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2009년도 제38회 동계학술대회 초록집
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• pp.180-180
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• 2010

Thin film transistors (TFTs) based on oxide semiconductors have emerged as a promising technology, particularly for active-matrix TFT-based backplanes. Currently, an amorphous oxide semiconductor, such as InGaZnO, has been adopted as the channel layer due to its higher electron mobility. However, accurate and repeatable control of this complex material in mass production is not easy. Therefore, simpler polycrystalline materials, such as ZnO and $SnO_2$, remain possible candidates as the channel layer. Inparticular, ZnO-based TFTs have attracted considerable attention, because of their superior properties that include wide bandgap (3.37eV), transparency, and high field effect mobility when compared with conventional amorphous silicon and polycrystalline silicon TFTs. There are some technical challenges to overcome to achieve manufacturability of ZnO-based TFTs. One of the problems, the stability of ZnO-based TFTs, is as yet unsolved since ZnO-based TFTs usually contain defects in the ZnO channel layer and deep level defects in the channel/dielectric interface that cause problems in device operation. The quality of the interface between the channel and dielectric plays a crucial role in transistor performance, and several insulators have been reported that reduce the number of defects in the channel and the interfacial charge trap defects. Additionally, ZnO TFTs using a high quality interface fabricated by a two step atomic layer deposition (ALD) process showed improvement in device performance In this study, we report the fabrication of high performance ZnO TFTs with a $Si_3N_4$ gate insulator treated using plasma. The interface treatment using electron cyclotron resonance (ECR) $O_2$ plasma improves the interface quality by lowering the interface trap density. This process can be easily adapted for industrial applications because the device structure and fabrication process in this paper are compatible with those of a-Si TFTs.

• 한국수자원학회논문집
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• 제37권12호
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• pp.1009-1018
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• 2004

하천을 따라 분포된 비점 오염원을 하천 수질모형의 매개변수들과 동시에 추정하는 체계적인 방법을 제안하였다. 수립된 방법을 QUAL2E 모형과 함께 충주댐 하류의 남한강 구간에 적용하여 모형의 반응계수와 비점 오염 부하량에 대한 최적 추정을 수행하였다. 민감도 분석 결과로부터 선정된 반응계수들에 대한 초기 추정 결과에 따르면 하천 시스템에 대한 질량수지가 만족되기 위해서는 질소와 인의 비점 오염 부하량의 입력이 필요한 것으로 나타났다. 이에 따라 총질소와 총인에 대한 비점 오염 부하를 포함하여 확장된 추정 문제의 해로써 비점 오염 부하량을 추정하였다. 비점 오염 부하량과 동시에 추정된 반응계수들과 비점 오염원을 고려하지 않고 추정된 반응계수의 비친 결과, 그 자체에 대한 추정을 위해서 뿐만 아니라 수질모형의 적절한 보정을 위하여 비점 오염 부하량이 최적 추정 과정에 포함될 필요가 있음을 알 수 있었다. 하천수질 의형에 대한 최적추정 방법의 적용성을 민감도계수 행렬 구조의 관점에서 논하였다.

• 한국정보디스플레이학회:학술대회논문집
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• 한국정보디스플레이학회 2008년도 International Meeting on Information Display
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• pp.1261-1262
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• 2008

Laser-based crystallization techniques are ideally-suited for forming high-quality crystalline Si films on active-matrix display backplanes, because the highly-localized energy deposition allows for transformation of the as-deposited a-Si without damaging high-temperature-intolerant glass and plastic substrates. However, certain significant and non-trivial attributes must be satisfied for a particular method and implementation to be considered manufacturing-worthy. The crystallization process step must yield a Si microstructure that permits fabrication of thin-film transistors with sufficient uniformity and performance for the intended application and, the realization and implementation of the method must meet specific requirements of viability, robustness and economy in order to be accepted in mass production environments. In recent years, Low Temperature Polycrystalline Silicon (LTPS) has demonstrated its advantages through successful implementation in the application spaces that include highly-integrated active-matrix liquid-crystal displays (AMLCDs), cost competitive AMLCDs, and most recently, active-matrix organic light-emitting diode displays (AMOLEDs). In the mobile display market segment, LTPS continues to gain market share, as consumers demand mobile devices with higher display performance, longer battery life and reduced form factor. LTPS-based mobile displays have clearly demonstrated significant advantages in this regard. While the benefits of LTPS for mobile phones are well recognized, other mobile electronic applications such as portable multimedia players, tablet computers, ultra-mobile personal computers and notebook computers also stand to benefit from the performance and potential cost advantages offered by LTPS. Recently, significant efforts have been made to enable robust and cost-effective LTPS backplane manufacturing for AMOLED displays. The majority of the technical focus has been placed on ensuring the formation of extremely uniform poly-Si films. Although current commercially available AMOLED displays are aimed primarily at mobile applications, it is expected that continued development of the technology will soon lead to larger display sizes. Since LTPS backplanes are essentially required for AMOLED displays, LTPS manufacturing technology must be ready to scale the high degree of uniformity beyond the small and medium displays sizes. It is imperative for the manufacturers of LTPS crystallization equipment to ensure that the widespread adoption of the technology is not hindered by limitations of performance, uniformity or display size. In our presentation, we plan to present the state of the art in light sources and beam delivery systems used in high-volume manufacturing laser crystallization equipment. We will show that excimer-laser-based crystallization technologies are currently meeting the stringent requirements of AMOLED display fabrication, and are well positioned to meet the future demands for manufacturing these displays as well.

• 대한방사성의약품학회지
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• 제3권2호
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• pp.54-58
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• 2017

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF MS) is one of the powerful methods that enable analysis of small molecules as well as large molecules up to about 500,000 Da without severe fragmentation. MALDI-TOF MS, thus, has been a very useful an analytical tool for the confirmation of synthetic molecules, probing PTMs, and identifying structures of a given protein. In recent nuclear medicine, MALDI-TOF MS liner ion mode helps researcher calculate the average number of chelator(or linkage) per an antibody conjugate, such as DOTA-(or DFO-) trastuzumab for labeling a medical radioisotope. This simple technique can be utilized to improve the labeling method and control the quality at the development of antibody-based radiopharmaceuticals, which is very effected to diagnosis and therapy for in vivo tumor cells, with radioisotopes like $^{89}Zr$, $^{64}Cu$, and 177Lu. To minimize the error, MALDI-TOF MS measurement is repeatedly performed for each sample in this study, and external calibration is carried out after data collection.

• Nuclear Engineering and Technology
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• 제54권7호
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• pp.2579-2585
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• 2022

Ceramics of quaternary garnets (Gd,Y)₃Al₂Ga₃O₁₂ doped with Ce, Tb have been fabricated and evaluated as prospective materials for indirect energy converters of α-and β-voltaic. Samples were characterized at excitation with an X-ray source and an intense 150 keV electron beam and showed good temperature stability of their emission and tolerance to irradiation. The role of X-rays accompanied the α-particle emitting in the increase of the conversion efficiency is clarified. The garnet-type structure of the matrix in the developed materials allows the production of quality crystalline mass with a light yield exceeding that of the commonly used YAG: Ce scintillator by a factor of two times.