• KSCE Journal of Civil and Environmental Engineering Research
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• v.9 no.3
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• pp.107-113
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• 1989

The purpose of this study is to compare the results of shear-deformation of saturated sand under the 3 dimensional stress with the results of simple torque-shear test already reported, Japaness standard sand, Toyoura sand, was chosen as test sample and the equipments of the department of soil mechancis laboratory of Nihon University were used. The conclusions obtained are as follows. 1). The friction angle of sand (${\phi}$) is proportional to the density regardless of the condition of stress-strain. This is because of the reason that the lower the cell pressure becomes, the larger the volume changes in case of the same density. 2). The value of ${\varphi}$ are variable according to the condition of stress-strain in the same density, and ${\phi}_dTS$ is larger than ${\phi}_dPS$ and ${\phi}_dTC$ when cell pressure is low. 3). ${\phi}_dPS$ is larger then ${\phi}_dTS$, under the same denstiy and same cell pressure. Thus the shear strength of sand is decided according to the condition of stress-strain 4). the relationship between the stress ratio (q/p) and strain increment ration in the plane strain test is linear regardless of the density and the cell pressure of the test sample.

• Animal Bioscience
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• v.34 no.10
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• pp.1590-1599
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• 2021

Objective: This study investigates the expression patterns of toll-like receptors (TLRs) and intracellular mediators in horse muscle cells after exercise, and the relationship between TLRS expression in stressed horse muscle cells and immune cell migration toward them. Methods: The expression patterns of the TLRs (TLR2, TLR4, and TLR8) and downstream signaling pathway-related genes (myeloid differentiation primary response 88 [MYD88]; activating transcription factor 3 [ATF3]) are examined in horse tissues, and horse peripheral blood mononuclear cells (PBMCs), polymorphonuclear cells (PMNs) and muscles in response to exercise, using the quantitative reverse transcription-polymerase chain reaction (qPCR). Expressions of chemokine receptor genes, i.e., C-X-C motif chemokine receptor 2 (CXCR2) and C-C motif chemokine receptor 5 (CCR5), are studied in PBMCs and PMNs. A horse muscle cell line is developed by transfecting SV-T antigen into fetal muscle cells, followed by examination of muscle-specific genes. Horse muscle cells are treated with stressors, i.e., cortisol, hydrogen peroxide (H₂O₂), and heat, to mimic stress conditions in vitro, and the expression of TLR4 and TLR8 are examined in stressed muscle cells, in addition to migration activity of PBMCs toward stressed muscle cells. Results: The qPCR revealed that TLR4 message was expressed in cerebrum, cerebellum, thymus, lung, liver, kidney, and muscle, whereas TLR8 expressed in thymus, lung, and kidney, while TLR2 expressed in thymus, lung, and kidney. Expressions of TLRs, i.e., TLR4 and TLR8, and mediators, i.e., MYD88 and ATF3, were upregulated in muscle, PBMCs and PMNs in response to exercise. Expressions of CXCR2 and CCR5 were also upregulated in PBMCs and PMNs after exercise. In the muscle cell line, TLR4 and TLR8 expressions were upregulated when cells were treated with stressors such as cortisol, H₂O₂, and heat. Migration of PBMCs toward stressed muscle cells was increased by exercise and oxidative stresses, and combinations of these. Treatment with methylsulfonylmethane (MSM), an antioxidant on stressed muscle cells, reduced migration of PBMCs toward stressed muscle cells. Conclusion: In this study, we have successfully cultured horse skeletal muscle cells, isolated horse PBMCs, and established an in vitro system for studying stress-related gene expressions and function. Expression of TLR4, TLR8, CXCR2, and CCR5 in horse muscle cells was higher in response to stressors such as cortisol, H₂O₂, and heat, or combinations of these. In addition, migration of PBMCs toward muscle cells was increased when muscle cells were under stress, but inhibition of reactive oxygen species by MSM modulated migratory activity of PBMCs to stressed muscle cells. Further study is necessary to investigate the biological function(s) of the TLR gene family in horse muscle cells.

• The Journal of the Korea Contents Association
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• v.20 no.5
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• pp.327-337
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• 2020

This study analyzed subjective cognitive types of culinary majors by applying TBL of cooking practice subjects, and applied Q methodology to multifaceted analysis of subjective cognitive types of learners. For the analysis of the study, interviews were conducted for college students majoring in cooking, and the survey was conducted in the order of constructing the Q population, selecting P-samples, classifying Q, interpreting the results, conclusions, and discussion. A total of four types were derived from the type analysis, and each was named according to its specificity as follows. Type 1 (N = 8): Cooperative Learning Effect Types, Type 2 (N = 8): Problem Solving Ability Effect Types, Type 3 (N = 6): Self Directed Learning Effect Type, Type 4 (N = 6): Individual Practice Preference Type analyzed for each unique feature type. It is expected that through the results of the study, it is expected to provide important implications that can help in the study of similar teaching methods in the future by fostering talents who can increase the needs of the industry and social stress.

• Journal of the Korean Applied Science and Technology
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• v.33 no.1
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• pp.41-50
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• 2016

In this study, the extracts from the leaves of Rhaphiolepis indica(R. indica) and Quercus salicina(Q. salicina) confirmed antioxidative effects. The antioxidative and cytotoxic effects of the two extracts were analyzed according to varied concentrations and time. The extracts of R. indica and Q. salicina showed dose-dependent DPPH radical scavenging activities. The extracts of R. indica and Q. salicina at concentrations of 5 mg/mL showed DPPH radical scavenging activities at 89.93 and 92.41%. Therefore, Q. salicina were confirmed to have higher antioxidative effects than R. indica. Total phenolic contents were 65.20 mg GAE/g for R. indica and 85.20 mg GAE/g for Q. salicina. The result that Q. salicina have higher total phenolic contents than R. indica suggested a correlation between total phenolic contents and DPPH radical scavenging activity. The cell protective effects of HepG2 and A549 cells under oxidative stress, both the extracts showed relatively low cell protective effects at around 10%. The Cytotoxic effects of A549 Cells did not show cytotoxicity at concentrations of $100{\mu}g/mL$ or below. The results of this study are likely to be used as basic data to develop antioxidants using the extract of R. indica and Q. salicina.

• Journal of Microbiology and Biotechnology
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• v.30 no.12
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• pp.1876-1884
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• 2020

Ethanol often accumulates during the process of wine fermentation, and mitophagy has critical role in ethanol output. However, the relationship between mitophagy and ethanol stress is still unclear. In this study, the expression of ATG11 and ATG32 genes exposed to ethanol stress was accessed by real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR). The result indicated that ethanol stress induced expression of the ATG11 and ATG32 genes. The colony sizes and the alcohol yield of atg11 and atg32 were also smaller and lower than those of wild type strain under ethanol whereas the mortality of mutants is higher. Furthermore, compared with wild type, the membrane integrity and the mitochondrial membrane potential of atg11 and atg32 exhibited greater damage following ethanol stress. In addition, a greater proportion of mutant cells were arrested at the G1/G0 cell cycle. There was more aggregation of peroxide hydrogen (H2O2) and superoxide anion (O2•-) in mutants. These changes in H2O2 and O2•- in yeasts were altered by reductants or inhibitors of scavenging enzyme by means of regulating the expression of ATG11 and ATG32 genes. Inhibitors of the mitochondrial electron transport chain (mtETC) also increased production of H2O2 and O2•- by enhancing expression of the ATG11 and ATG32 genes. Further results showed that activator or inhibitor of autophagy also activated or inhibited mitophagy by altering production of H2O2 and O2•. Therefore, ethanol stress induces mitophagy which improves yeast the tolerance to ethanol and the level of mitophagy during ethanol stress is regulated by ROS derived from mtETC.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.197-197
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• 2022

Wheat is highly susceptible to heat stress, which significantly reduces grain yield. In this study, we used RNA-seq technology to analyze the transcript expression at three different time-points after heat treatment in three cultivars differing in their susceptibility to heat stress: Jopum, Keumkang, and Olgeuru. A total of 11,751, 8850, and 14,711; 10,959,7946, and 14,205; and 22,895,13,060, and 19,408 differentially-expressed genes (log2 fold-change > 1 and FDR (padj) < 0.05) were identified in Jopum, Keumkang, and Olgeuru in the control vs. 6-h, in the control vs. 12-h, and in the 6-h vs. 12-h heat treatment, respectively. Functional enrichment analysis showed that the biological processes for DEGs, such as the cellular response to heat and oxidative stress-and including the removal of superoxide radicals and the positive regulation of superoxide dismutase activity-were significantly enriched among the three comparisons in all three cultivars. Furthermore, we investigated the differential expression patterns of reactive oxygen species (ROS)-scavenging enzymes, heat shock proteins, and heat-stress transcription factors using qRT-PCR to confirm the differences in gene expression among the three varieties under heat stress. This study contributes to a better understanding of the wheat heat-stress response at the early growth stage and the varietal differences in heat tolerancea.

• Geomechanics and Engineering
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• v.37 no.3
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• pp.213-222
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• 2024

Determination of jointed rock mass properties plays a significant role in the design and construction of underground structures such as tunneling and mining. Rock mass classification systems such as Rock Mass Rating (RMR), Rock Mass Index (RMi), Rock Mass Quality (Q), and deformation modulus (Em) are determined from the jointed rock masses. However, parameters of jointed rock masses can be affected by the tunnel depth below the surface due to the effect of the in situ stresses. In addition, the geomechanical properties of rocks change due to the effect of metamorphism. Therefore, the main objective of this study is to apply correlation analysis to investigate the relationships between rock mass properties and some parameters related to the depth of the tunnel studied. For this purpose, the field work consisted of determining rock mass parameters in a tunnel alignment (~7.1 km) at varying depths from 21 m to 431 m below ground surface. At the same excavation depths, thirty-seven rock types were also sampled and tested in the laboratory. Correlations were made between vertical stress and depth, horizontal/vertical stress ratio (k) and depth, k and Em, k and RMi, k and point load index (PLI), k and Brazilian tensile strength (BTS), Em and uniaxial compressive strength (UCS), UCS and PLI, UCS and BTS. Relationships were significant (significance level=0.000) at the confidence interval of 95% (r = 0.77-0.88) between the data pairs for the rocks taken from depths greater than 166 m where the ratio of horizontal to vertical stress is between 0.6 and 1.2. The in-situ stress parameters affected rock mass properties as well as metamorphism which affected the geomechanical properties of rock materials by affecting the behavior of minerals and textures within rocks. This study revealed that in-situ stress parameters and metamorphism should be reviewed when tunnel studies are carried out.

• Structural Engineering and Mechanics
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• v.52 no.1
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• pp.89-95
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• 2014

We analyze the buckling of a thin elastic plate due to intrinsic stresses in thin films attached to the surfaces of the plate. In the case of cylindrical buckling, it is shown that for a plate with clamped edges compressive intrinsic film stresses can cause flexural buckling of the plate, while tensile intrinsic film stresses cannot. For a plate with free edges, film intrinsic stresses, compressive or tensile, cannot cause buckling.

• Proceedings of the Earthquake Engineering Society of Korea Conference
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• 2000.04a
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• pp.28-35
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• 2000

The quality factor and the seismic source parameters such as the $\chi$ corner frequency and the stress drop were estimated from the small-to-medium instrumental earthquake data in south Korea. The Q facter with 95% confidence level ranges from 1519 to 2158. The regression equation of $\chi$in terms of epicentral distance R, is obtained as $\chi$=0.006717+0.00015R. And the stress drop is estimated as 50 bar which is similar to the previous results carried out by independent researchers. Artificial ground motions were simulated using the estimated earthquake parameter values and compared with real earthquake, The simulated response spectrum is very similar to real one.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2009.10a
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• pp.119-123
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• 2009

In this paper, effects of major design parameters of cold forging dies on die mechanics are quantitatively investigated with emphasis on shrink fit using a thermoelastic finite element method. A ball-stud cold forging process found in a cold forging company is selected as a test process and the effects of die insert material, shrink fit, dimension of ring, partition of die inert and clamping force on effective stress and circumferential stress are analyzed.