• 생명과학회지
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• 제29권2호
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• pp.191-197
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• 2019

파킨슨병은 운동완서, 근육경직, 진전 및 비정상적인 자세 등을 임상적 특징으로 하는 주로 운동 신경계에 영향을 주는 진행성 신경 퇴행성 질환이다. 파킨슨병은 산화 스트레스와 세포 내 신호 전달 경로의 조절 장애에 의한 뇌 흑색치밀부에서의 도파민성 신경세포의 사멸을 특징으로 한다. Quercetin의 주요 대사산물인 Quercetin-3-O-glucuronide (Q3GA)는 신경 보호 효과가 있는 것으로 보고 되어 왔다. 본 연구에서는 SH-SY5Y 세포에서 1-methyl-4-phenyl pyridinium ($MPP^+$)에 의해 유도된 신경 독성에 대한 Q3GA의 신경 보호 효과와 그 분자 조절 기전을 조사하였다. Q3GA는 $MPP^+$에 의해 유도된 세포 사멸을 유의적으로 감소시켰으며 PARP 절단을 감소시켰다. 또한, Bax/Bcl-2 비율의 감소와 함께 $MPP^+$에 의해 증가된 세포 내 ROS를 감소시켰다. Q3GA는 $MPP^+$에 의해 감소된 Akt와 CREB의 인산화를 유의적으로 회복시켰지만, ERK에는 영향을 미치지 않았다. 이 결과는 Q3GA가 ROS 생산 억제와 Akt/CREB 신호 전달 경로를 통해 $MPP^+$ 에 의해 유도된 신경 독성을 억제시킬 수 있음을 시사한다. 본 연구는 Q3GA가 파킨슨병에 대한 예방제 또는 치료제로 개발될 수 있는 가능성을 제시한다.

• Composites Research
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• 제31권4호
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• pp.145-150
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• 2018

항공우주산업에서 구조물의 수명연장과 경제적 측면에서의 효율적인 운용을 위해 다양한 구조건전성모니터링(Structural Health Monitoring, SHM) 기법들이 제시되어왔다. 금속재 구조물의 경우, 수분이나 염분 등에 의한 부식이나 쉽게 응력집중이 발생하는 타공, 노치, 볼트 등과 같은 위치에서의 균열이 주된 관심사였으나, 항공우주산업에서의 복합재 사용비율이 증가함에 따라 손상 메커니즘이 더욱 복잡한 복합재 구조물에 적용이 가능한 고도화된 SHM 시스템의 필요성이 강조되고 있다. 본 논문에서는 Q-switched 레이저와 다수의 압전센서를 이용한 복합재에서의 AE(Acoustic emission) 위치탐지 기법을 제시한다. 제시되는 기법은 10 mm 이내의 거리오차로 방출위치 탐지를 목표로 하며 복합재 구조에서 수행된 AE 모사실험 및 위치탐지 시도 결과를 제시하여 기법이 유효함을 증명한다.

• 한국기계가공학회지
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• 제14권2호
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• pp.1-6
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• 2015

Transmission of a tracked vehicle designed for multiple functions such as steering, gear-shifting, and braking is a core component of heavy vehicle to which the power is transferred based on combined technology of various gears, bearing, and fluid machineries. Robustness and durability of transmission, however, have been issued due to a large number of driving units and sub-components inside its body. The bevel gears are major components for the transmission of power in a transmission. Increasing the tooth surface roughness and chamfering of the bevel gears, especially, we aim to improve the quality of transmission. In this study, design structural evaluation is conducted on bevel gears of transmission for tracked vehicle using the ROMAX-DESIGNER program. By doing so, design safety of the bevel gears has been evaluated based on the gear strength theory of ANSI/AGMA 2003 B97 standard.

• 한국강구조학회 논문집
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• 제13권2호
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• pp.123-131
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• 2001

본 연구의 목적은 고강도 H형강 기둥재(beam-column)의 폭두께비에 관한 기준을 조사하고 좌굴내력을 평가하여, 강구조 한계상태설계기준과 허용응력설계기준(안)과 비교함으로써, 고강도강을 사용한 기둥부재 설계시 적용된 기준식의 타당성을 검토하기 위한 것이다. 실험에 사용된 고강도강은 SM520TMC, SM570Q 등을 사용하였고, 강재의 기계적 성질과 단주의 응력-변형도 관계를 파악하기 위하여 인장시험 및 단주압축시험을 실시하였다. 또한 고강도강 기둥재의 좌굴내력을 산정하기 위하여 수치해석을 수행하였다. 수치해석에 사용된 축력-모멘트-곡률 관계는 단주압축실험에서 구해진 응력-변형도 관계를 사용하였다

• Fisheries and Aquatic Sciences
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• 제21권8호
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• pp.28.1-28.12
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• 2018

Intertidal macroalgae are exposed to many abiotic stress factors, and they must regularly react to changes in their environment. We used RNA-seq to describe how Porphyra umbilicalis (Rhodophyta) changes gene expression patterns to interact with different habitats. Tissue samples were taken from a typical habitat along the open-coast of the Northwest Atlantic, as well as from a rare, atypical habitat in an estuarine tidal rapid environment. Differential gene expression analyses suggest that pathogic bacteria and viruses may be a significant factor influencing the transcriptome in the human-impacted estuarine environment, but the atypical habitat does not necessarily induce more stress in Porphyra umbilicalis growing there. We found genes related to nitrogen transport are over-expressed in tissue from the open-coastal site compared to those from the estuarine site, where environmental N levels approach hypertrophic levels. Low N levels impede growth, but high levels are toxic to cells, and we use qPCR to show this species regulates expression of a putative high-affinity $NH_4{^+}$ transporter under low and high N conditions. Differences in expression of this transporter in these habitats appear to be inherited from parent to offspring and have general implications for adaptation to habitat in other species that are capable of asexual reproduction, as well as more specific implications for this species' use in aquaculture.

• 수산해양교육연구
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• 제27권4호
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• pp.1063-1074
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• 2015

Nursing students are often embarrassed about offering an intimate care to patients in a clinical environment, and experience stress. In particular, nursing students is often a low level of interaction skill and nursing school has no education program for this intimate care. Therefore, these difficulties are increased while the clinical practice. The purpose of this study was to identify the attitude of female nursing students regarding intimate care to patient and the characteristics of the type by applying Q-methodology, and to create the basic data for the development of education programs for nursing student to prevent stress and embarrassed feeling. Data were collected by applying 35 Q statements to 32 female nursing students. Collected data were analyzed with the PC QUANL program. Results indicate that female nursing students' attitudes of intimate care were divided into four types: 'considering situation-professional obligation type' 'gender related risk avoidance type' 'transcendent professional care type' and 'pursuit of the ideal professional type.' The types of female nursing students' attitudes of intimate care to patient were discussed, focusing on the attitude type identified in this study.

• 국제강구조저널
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• 제18권5호
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• pp.1784-1800
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• 2018

Tension-shear loading is a common loading condition in steel structures during the earthquake shaking. To study ductile fracture in structural steel under multiple stress states, experimental investigations on the different fracture mechanisms in Chinese Q235 steel were conducted. Different tension-shear loading conditions achieved by using six groups of inclined notch butterfly configurations covering pure shear, tension-shear and pure tension cases. Numerical simulations were carried out for all the specimens to determine the stress and strain fields within the critical sections. Two tension-shear fracture models were calibrated based on the hybrid experimental-numerical procedure. The equivalent fracture strain obtained from the round bar under tensile loading was used for evaluating these two models. The results indicated that the tension-shear criterion as a function of the shear fracture parameter had better performance in predicting the fracture initiation of structural steel under different loading conditions.

• Plant Breeding and Biotechnology
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• 제5권4호
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• pp.363-370
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• 2017

In this study, Quercus acutissima seedlings were subjected to drought for 30 days then analyzed to determine their response to water deficit. The growth phenotype, chlorophyll fluorescence response, fresh weight, dry weight, photosynthetic pigment levels, soluble sugar content, and malondialdehyde (MDA) were measured to evaluate the effects of drought on plant growth and physiology. The growth phenotype was observed by infrared (IR) digital thermal imaging after 30 days of drought treatment. The maximum, average, and minimum temperatures of drought-treated plant leaves were $1-2^{\circ}C$ higher than those of the control. In contrast, the fresh and dry weights of the dehydrated leaves were generally lower than those of the control. There were no significant differences between treatments in terms of chlorophyll a, chlorophyll b, total chlorophyll, and carotenoid levels. Nevertheless, for the drought treatment, the $F_v/F_m$ and $F_v/F_o$ ratios (chlorophyll fluorescence response) were lower than those for the control. Therefore, photosynthetic activity was lower in the dehydrated plants than the control. The drought-stressed Q. acutissima S0536 had lower soluble sugar (glucose and fructose) and higher MDA levels than the controls. These findings may explain the early growth and physiological responses of Q. acutissima to dehydration and facilitate the selection of drought-resistant tree families.

• Journal of Preventive Medicine and Public Health
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• 제38권3호
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• pp.345-350
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• 2005

Objectives : Polycyclic aromatic hydrocarbons (PAHs), which are risk factors for lung cancer, have been reported to induce oxidative DNA damage. The paraoxonase (PON) plays a significant role in the detoxification of a variety of organophosphorous compounds, with paraoxonase-1 (PON1) being one of the endogenous free-radical scavenging systems in the human body. The aim of this case-control study was to investigate the effects of PAH exposure, oxidative stress and the Q192R polymorphism of PON1 genes, and their interactions in the carcinogenesis of lung cancer. Methods : One hundred and seventy seven lung cancer patients and 177 age- and sex-matched controls were enrolled in this study. Each subject was asked to complete a questionnaire concerning their smoking habits and environmental exposure to PAHs. The Q192R genotypes of the PON1 gene was examined, and the concentrations of urinary 1-hydroxypyrene (1-OHP), 2-naphthol and 8-hydroxydeoxyguanosine (8-OH-dG) measured. Results : Cigarette smoking was found to be a significant risk factor for lung cancer. The urinary 8-OH-dG level was higher in the patients, whereas the urinary 1-OHP and 2-naphthol levels were higher in the controls. There was a significant correlation between the urinary levels of 8-OHdG and 1-OHP in both the cases and controls. The PON1 polymorphism was associated with an increased risk of lung cancer. Individuals carrying the Q/Q genotype of the PON1 gene were found to be at higher risk of developing lung cancer. There was a significant correlation between the urinary levels of 8-OH-dG and 1-OHP in those with the PON1 Q/Q genotype. Conclusions : These results lead to the conclusion that PAHs would induce oxidative DNA damage, especially in individuals with the PON1 Q/Q genotype. Therefore, people with the PON1 Q/Q genotype would be more susceptible to lung cancer than those with the R/R or Q/R genotypes of the PON1 gene.

• 한국지반공학회:학술대회논문집
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• 한국지반공학회 2010년도 춘계 학술발표회
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• pp.730-739
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• 2010

Stress history increases in penetration resistance due to the increase in residual horizontal stress of granular soil. This study analyzes the effect of stress history on the results of CPT and DMT from calibration chamber specimen in OC as well as NC state. Test results show that the normalized cone resistance by mean effective stress correlates well with the relative density and the state parameter, whereas the normalized cone resistance with regard to vertical effective stress is a little affected by stress history. The horizontal stress index($K_D$) in DMT more reflects the influence of stress history on granular soil than the dilatometer modulus($E_D$) and cone resistance($q_c$). The $K_D/K_0$, in which the effect of stress history on $K_D$ is compensated by the at-rest coefficient of earth pressure, $K_0$, is related to relative density, state parameter and the normalized cone resistance by mean effective stress. It is also observed that the normalized dilatometer modulus by mean effective stress($E_D/{\sigma_m}'$) is unique correlated with the state parameter, regardless of stress history.