• 보존과학회지
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• 제1권1호
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• pp.12-26
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• 1992

Ilsan peat-bed site, newly constructed city area, is located at the northern part of Seoul. This site is situated near the mouth of Han river, which flows through central Korea. Many fossil woods were excavated from this site, and surveyed the wood anatomical features. Totally 90 specimens were identified into 10 species including two roots and five barks which were not identified in detail. Alnus japonica and A. maxmowiczii are the most common and occupied 26. 7%, 34.4% of the total specimen number respectively, and then other species which excavated from Ilsan area are follows : Fraxinus rhynchophylla(18.9%), Lepido-balanus spp.(3.3%), Maackia amurensis(2.2%), Diospyros spp.(2.2%), Juglans mandshrica(1.1%), Prunophora spp.(1.1%), Malus or Pyrus spp.(1.1%), Rhamnus spp.(1.1%). Among the identified species there are fairly some species growing in the central - or northern temperate zone, such as A. maxmowiczii, F. rhynchophylla, Lepidobalanus spp., M. amurnsis, J. mandshurica, Rhumnus spp. Therefore, it can be estimated that the former temperature of Ilsan area was same or slightly cold than today. The species bearing fruit for food as .J, mandshunca, Diospyros spp., Prunophora spp., Mulus or Pyrus spp. were regarded as artificially planting trees, because they do not grow together in the same site.

• Journal of Plant Biotechnology
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• 제42권4호
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• pp.290-297
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• 2015

배나무는 원산지와 분화방향에 따라 유럽, 미국, 호주 등에서 주로 재배되는 서양배와 중국, 일본, 한국 등 동남 아시아 지역을 중심으로 분포 및 재배되고 있는 동양배로 구분된다. 17개의 기본염색체를 가진 배나무는 대부분 이배성(2n=2x=34)이며, 단일 S 유전자좌에 의해 조절되는 자가불화합성과 과수 작물의 주요 특징인 유년성으로 인해 유전 연구 및 정밀한 품종 육성에 큰 제한을 받고 있다. 배나무속 식물의 유전연구는 분자생물학 관련 기술의 발달로 다양한 형태의 분자 표지의 개발이 이루어짐과 동시에 유연관계분석, 유전자지도작성, QTL 분석과 같은 다양한 유전연구에 활발히 이용되었다. 또한 배나무의 유전자지도는 병 저항성이나 다양한 유용형질과 연관된 QTL 확인을 위한 연구로 이어지고 있다. 대량 병렬 반응 및 다중처리를 토대로 획기적인 염기서열 분석 비용의 감소를 이뤄낸 NGS 기술은 대용량, 고효율, 저비용으로 식물 유전체 해독을 가능하게 하여, 중국배 'Danshansuli'와 유럽배 'Bartlett'에서 유전체 분석이 완료되었다. 최근 국내에서는 황금배, 청실리 및 미니배의 resequencing 및 GBS를 통한 SNP 탐색 등의 연구를 통해 화기, 숙기 당도 등 농업적으로 유용형질에 대한 게놈전체 연관분석을 수행하고 있다.

• 한국자원식물학회지
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• 제35권6호
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• pp.770-777
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• 2022

The main objective of this study was to identify the most appropriate condition for root formation of in vitro micropropagated pear (Pyrus spp.) plants. In vitro propagation was induced on Murashige and Skoog (MS) medium with 2.0 mg/L of N6-benzyladenine (BA) and 0.2 mg/L of Indole-3-butyric acid (IBA) medium. The short pre-treatment of explants with a high concentration (1 mg/L) of NAA and IBA (R0 medium) in dark for three days, followed by transfer to five different media (R1 to R5) resulted in good rooting responses in the pear 'Oharabani (P. pyrifolia × P. communis)' genotype. For the rooting experiments, the highest rooting percentage (83.3 ± 8.3%), average root length (3.6 ± 1.9 mm), total root number (31 ± 4.0), and average root number per plant (2.6 ± 2.1) were obtained on half strength (1/2) of MS medium supplemented with 30 g/L sucrose without hormones and activated charcoal (AC) (R1 medium). The highest rooting percentage was obtained at 83.3% from explants on R1 and R3 media. The rooting procedure described in this study resulted in good root formation and significantly shorting the root induction time to within 14 days of culture. Further studies are underway to test the suitability of the protocol developed in this study for other pear genotypes.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2022년도 추계학술대회
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• pp.35-35
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• 2022

Pears (Pyrus spp.) have been grown worldwide as a kind of important economical fruits. Over 1,500 accessions collected from countries have been preserved in National Institute of Horticultural and Herbal Science, Rural Development Administration in Korea. However, redundancies and misidentification are happening in the germplasm preservation due to same cultivars which have different names in various localities (synonyms) and different cultivars which have same names (homonyms). That can lower germplasm management efficiency. The object of this study is to identify synonyms and homonyms in pear germplasms by analyzing genetic variation with four microsatellite markers: CH03d12, CH03g07, CH02b10, and EMPc117. PCR amplification with above 4 microsatellite markers was done for the 31 pear accessions, and the products were analyzed by agarose gel electrophoresis. As a result, 7 synonyms and 9 homonyms were identified among 31 pear accessions. We'll compare these genotypes with phenotypes of each pear accessions, and reduces the redundancy and misidentification in pear germplasm collection for the reliable management.

• 한국자원식물학회지
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• 제36권6호
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• pp.571-579
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• 2023

In this study, cryopreservation by droplet-vitrification was applied to pear (Pyrus spp.) germplasm. We focused on the development and assessment of various strategies for the selection of suitable tissue, osmoprotection, and dehydration. We also evaluated post-thaw recovery of cryopreserved explants by droplet-vitrification. Preferentially, we tested the effects of preculture and loading treatments to determine which tissues were more suitable, either the apical shoot tips or the axillary buds. Apical shoot tips showed the better regrowth rate than in vitro axillary buds. The most effective techniques for cryopreservation were as follows. Shoots from in vitro seedlings which had been cultured for about 5-6 weeks were cold-hardened at 4℃ for one week, excised shoot tips were precultured on liquid MS medium including 0.3 M sucrose for 31 hours and 0.7 M sucrose for 17 hours, osmoprotected in loading solution (LS) for 40 min, and then cryoprotected in dehydration solution (PVS3) for 90 min. In addition, we found that regrowth rates of explants on regrowth medium after exposure to liquid nitrogen (LN) were higher than those on MS medium. Results indicated that the highest regrowth percentage was 95.6% for 'Bartlett' cultivar and 68.9% for 'BaeYun No.3' cultivar. Consequently, apical shoot tips of two pear cultivars, 'Bartlett' (P. communis) and 'BaeYun No.3' (P. pyrifolia), were successfully cryopreserved by droplet-vitrification. Results of this study show that the enhanced droplet-vitrification method described in the present study could be used as an effective means for long-term storage of pear genetic resources.

• Journal of Plant Biotechnology
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• 제4권2호
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• pp.45-52
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• 2002

A lot of SSRs (simple sequence repeats) in peach and pear from enriched genomic libraries and in peach from a cDNA library were developed. These SSRs were applied to other related species, giving phenograms of 52 Prunes and 60 pear accessions. Apple SSRs could also be successfully used in Pyrus spp. Thirteen morphological traits were characterized on the basis of the linkage map obtained from an $F_2$ population of peach. This map was compiled with those morphological markers and 83 DNA markers, including SSR markers used as anchor loci, to compare different peach maps. Molecular markers tightly linked to new root-knot nematode resistance genes were also found. A linkage map including disease related genes, pear scab resistance and black spot susceptibility, in the Japanese pear Kinchaku were constructed using 118 RAPD markers. Another linkage map, of the European pear Bartlett, was also constructed with 226 markers, including 49 SSRs from pear, apple, peach and cherry. Maps of other Japanese pear cultivars, i.e., Kousui and Housui, were also constructed. These maps were the first results of pear species.

• 한국육종학회지
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• 제41권4호
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• pp.444-450
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• 2009

본 연구는 배 유전자원의 유전적 변이를 DNA 수준에서 비교함으로써 육종의 기초 자료로 활용하기 위하여 유전자원 60점을 대상으로 AFLP 분석을 수행하였다. 총 20종의 AFLP 프라이머 조합을 이용하여 522개의 다형성 밴드를 얻었다. 획득된 다형성 밴드를 이용하여 UPGMA 방식으로 유사도 및 집괴분석을 수행한 결과 유전적 유사도 0.691를 기준으로 4개의 그룹으로 분류되었다. 첫 번째 그룹에는 Pyrus communis에 속하는 품종 및 P. nivalis, P. cordata 등이 포함되었다. P. betulaefolia와 P. fauriei에 속하는 콩배 계통들이 두 번째 그룹에 속하였고, P. calleryana와 P. koehnei를 포함한 콩배 계통들이 세 번째 그룹으로 분류되었다. 네 번째 그룹에는 P. pyrifolia와 P. ussuriensis에 속하는 재배품종, 교잡종 및 그 외의 종들이 대부분 속하여 동아시아에서 유래한 유전자원들은 P. pyrifolia나 P. ussuriensis와 서로 밀접히 연관되어 있음을 확인할 수 있었다. 유전자원 간 유전적 유사도는 0.584에서 0.879범위로 평균 유전적 유사도는 0.686이었다.

• Journal of the Korean Wood Science and Technology
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• 제36권1호
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• pp.1-11
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• 2008

안성 석남사 영산전에 쓰인 주요 목부재의 수종을 식별하기 위하여 기둥, 창방, 대들보, 서까래, 청판 등 총 95점에 대하여 조사하였다. 수종은 소나무류(경송류), 외래산 소나무류, 느티나무, 은행나무, 감나무속, 배나무속, 참나무속의 상수리나무류 등 7 수종이 식별되었다. 기둥에서는 소나무류(경송류) 8점, 느티나무 1점, 배나무속 1점이 확인되었다. 추녀에서는 은행나무 2점과 느티나무 2점, 대들보에서는 소나무 1점과 느티나무 1점이 나왔다. 주심도리는 2점 모두 상수리나무류이었고, 서까래에서는 은행나무 1점과 외래산 소나무류 1점을 제외하고는 나머지는 소나무류인 것으로 밝혀졌다. 적심목에서 느티나무 1점과 감나무속 1점이 나왔다. 다른 부재는 소나무류가 대부분이었다. 서까래에서 나온 외래산 소나무 1점은 해방 이후 영산전 수리 시 사용된 것으로 추정되었다. 소나무류 이외에 다양한 수종의 목재가 사용된 것은 사찰 인근에서 쉽게 구할 수 있는 수종들을 이용하였기 때문인 것으로 사료된다.

• 한국식물병리학회지
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• 제11권3호
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• pp.210-216
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• 1995

Alternaria spp.were predominantly isolated from the abnormal leaf spot lesions of pear cultivars Niitaka and Nijiiseiki. Alternaria isolates from the cultivar Niitaka were not pathogenic to both cultivars, but the isolates from the cultivar Nijiiseiki developed typical lesions of black leaf spots and were identified as A. kikuchiana. However, no typical abnormal leaf spot lesions were produced by the Alternaria isolates. Foliar spray of twelve different agrochemicals including lime sulfur, either alone or in combinations, with 7 times applications from April to July failed to reduced the disease development. Application of 17 different pesticides including fungicides, insecticides and herbicides currently used in pear orchards did not cause leaf injury similar to the abnormal leaf spot. Simulated acid rain of as low as pH 3.0 did not incite any leaf lesions alike the abnormal spot lesions. Mineral contents in the leaves of both cultivars did not differ significantly between the healthy leaves and those with abnormal leaf spots. When cuttings of pear tree were obtained in February from newly emerged twigs of the healthy or the diseased trees of Niitaka and planted in sand in the greenhouse, only those from the diseased trees developed typical leaf lesions of the abnormal spot. These results indicate that abnormal leaf spots are caused by unknown systemic agents in pear trees, rather than by Alternaria spp., chemical injury or acid rain.

• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2002년도 추계학술대회
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• pp.101-109
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• 2002

A lot of SSRs (simple sequence repeats) in peach and pear from enriched genomic libraries and in peach from a cDNA library were developed. These SSRs were applied to other related species, giving phenograms of 52 Prunus and 60 pear accessions. Apple SSRs could also be successfully used in Pyrus spp. Thirteen morphological traits were characterized on the basis of the linkage map obtained from an $F_2$ population of peach. This map was compiled with those morphological markers and 83 DNA markers, including SSR markers used as anchor loci, to compare different peach maps. Molecular markers tightly linked to new root-knot nematode resistance genes were also found. A linkage map including disease-related genes, pear scab resistance and black spot susceptibility, in the Japanese pear Kinchaku were constructed using 118 RAPD markers. Another linkage map, of the European pear Bartlett, was also constructed with 226 markers, including 49 SSRs from pear, apple, peach and chewy. Maps of other Japanese pear cultivars, i.e., Kousui and Housui, were also constructed. These maps were the first results of pear species.