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ICP-MS와 MA-2를 이용한 버섯류 유해 중금속 모니터링

  • Han, Chang-Ho;Hwang, Gwang-Ho;Ko, Suk-Kyung;Kim, Soo-Jin;Kim, Hee-Soon;Kim, Yoo-Kyung;Park, Gun-Young;Kim, Dong-Yoon;Lim, Sang-Chul;Choi, Byung-Hyun;Kim, Min-Young
    • 한국약용작물학회:학술대회논문집
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    • 2010.10a
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    • pp.381-381
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    • 2010
  • 2010년 5월 중 서울 약령시와 서울시내 대형마트에서 유통 중인 약용버섯 및 식용버섯류 16품목 92건 대상으로 중금속(비소, 카드뮴, 납, 수은) 모니터링을 실시하였다. 식품의약품안전청에서 고시한 생약의 잔류오염물질 허용기준 및 시험방법에 따라 실험하였으며, 유통 버섯류의 중금속 함유실태를 파악하고, 버섯의 안전성 기준 제정을 위한 기초 자료를 제공하고자 본 실험을 실시하였다. 새송이버섯 등 생버섯 7종 15건에 대한 중금속 검사 결과 비소 0.01~0.26 mg/kg, 카드뮴 0~0.10 mg/kg, 납 0~0.03 mg/kg, 수은 0~0.007 mg/kg 으로 중금속 함유량이 낮았다. 상황버섯 등 11종 77건의 건조되어 식용 및 약용으로 사용되는 버섯류 중 노루궁뎅이버섯은 4건 중 2건(50.0 %)에서 카드뮴이 0.32~0.82 mg/kg으로 한약재 기준 대비 높게 검출되었으며, 상황버섯은 15건 중 5건(33 %)에서 카드뮴 0.58~0.85 mg/kg, 표고버섯은 9건 중 3건에서 카드뮴 0.42~0.74 mg/kg, 운지버섯 5건 중 1건(20.0 %)에서 1.66 mg/kg, 영지버섯 16건 중 2건(12.5%)에서 수은 0.415 mg/kg과 카드뮴 0.66 mg/kg이 분석되었다. 특히 아가리쿠스버섯은 10건 중 10건(100.0 %)에서 카드뮴(10건) 1.49~7.35 mg/kg, 수은(9건) 0.202~0.505 mg/kg으로 높은 유해중금속 함유량을 보이는 것으로 조사되었다.

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Survey of the current status of beverages sold from vending machines in subway stations in the Seoul metropolitan area, and their sugar content (서울지역 지하철역사 내 자판기 판매 음료현황 및 당류 함량 조사)

  • Kim, Donggyu;Lee, Mokyoung;Kim, Yeosook;Choi, Sujeong;Shin, Jaemin;Hwang, Youngsuk;Yun, Eunsun;Jo, Namsook;Kim, Junghun;Oh, Younghee;Jung, Kweon;Kwak, Jiyoung;Kim, Dahyun;Kim, Eunji;Park, Somi;Yang, Silbee;Cho, Hyelim;Ahn, Ryoungme
    • Analytical Science and Technology
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    • v.29 no.6
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    • pp.249-254
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    • 2016
  • Looking at the current status of beverages sold from vending machines in subway stations in Seoul, it was found that carbonated beverages accounted for the highest percentage of beverages on sale in vending machines, representing 36 % of all beverages sold. Survey respondents purchased carbonated drinks or sports drinks containing high levels of sugar to quench their thirst. Investigation of the sugar content of beverages frequently sold in vending machines showed that a serving of carbonated beverage contained an average of 30.4 g of sugar, or as much as 50.1 g of sugar (equivalent 10 to 17 cubes; 1 cube = 3 g of sugar). This amount corresponded, on average, with 60 % of the WHO recommended daily sugar intake (50 g). Surprisingly, with certain carbonated beverages, drinking a single can is equivalent to consuming the total recommended daily sugar intake of 50 g.

Utilization and Needs Assessment of Health Promotion Programs for Middle-aged Women in Public Health Centers (중년 여성의 보건소 건강증진 프로그램 이용 실태 및 요구도)

  • Choi, Eun-Jin;Yoo, Ji-Soo;Kim, Hee-Soon;Oh, Eui-Geum;Kim, Sue;Bae, Sun-Hyung;Chu, Sang-Hui
    • Journal of Korean Public Health Nursing
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    • v.21 no.2
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    • pp.193-205
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    • 2007
  • Purpose: To evaluate the current status of utilization and implementation of health promotion programs for Korean middle-aged women in public health centers. Methods: Three-phase stratified sampling was done to select 1304 middle-aged women (aged 40-64 years) from all regions of Korea. The data were collected by face-to-face interviews using a structured questionnaire for individual responses and mailed surveys to 126 public health centers. Descriptive statistics and $x^2$-test were done for data analysis. Results: Only 12 of the 126 public health centers (9.9%) implemented health promotion programs for middle-aged women, with the lack of manpower being cited as the main reason for the absence of programs. From individual responses, 11.3% had participated in health promotion programs offered by public health centers. The main reasons for not participating were inconvenient times and lack of information. Significant differences were found in the frequency of participation in programs, exercise programs and diabetes management according to the size of region. The majority of the respondents cited the need for medical services, followed by programs focused on stroke prevention and leisure time management. The responses on the willingness to participate followed a similar pattern. Conclusion: There are gaps between the utilization of health promotion programs by middle-aged women and what is offered by public health centers. The results of this study support the need to develop more health promotion programs focusing specifically on the needs of middle-aged women.

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A Study on Contents of Sugars and Sugar Alcohols in Processed Foods Met to Children's Taste (어린이기호식품 중 당류 및 당알코올류 함량분석)

  • Kum, Jin-Young;Hong, Mi-Sun;Jang, Mi-Ra;Choi, Bu-Chuhl;Lee, Kyeong-Ah;Kim, Il-Young;Kim, Jung-Hun;Chae, Young-Zoo
    • Journal of Food Hygiene and Safety
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    • v.29 no.3
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    • pp.241-247
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    • 2014
  • In this study, HPLC-RI was used to determine sugars and sugar alcohols contents in 102 different kinds of processed foods met to children's taste. The average amount of sugars per 100 g of candies was 70.25 g, of processed cocoa products was 65.34 g, of processed chocolate products was 47.53 g, of breads was 25.66 g, of cookies was 22.28 g, of ices was 12.47 g, of snacks was 9.74 g. Processed cocoa product items had relatively higher sucrose contents (56.80 g/100 g) than any other items. The average amount of sucrose per 100 g of candies was 44.20 g, of processed chocolate products was 32.89 g, of breads was 23.11 g. When the contents of sugars in processed foods met to children's taste per on serving size were compared to WHO guidelines, the percentages were 5.84~28.52 about recommended daily intake of total sugar of 50 g. The result for the analysis of confectioneries showed that 13 samples of 102 were detected and the sugar alcohols content of samples investigated varied between 0.01~15.06%.

A Survey on Ochratoxin a Contamination of Commercial Medicinal Herbs in Incheon (인천지역 유통 생약 중 Ochratoxin A 오염실태 조사)

  • Lim, Soo-sun;Joo, Gwang-sig;Choi, Eun-Jeong;Lee, Soo-Yeon;Lim, Yun-sun;Eum, Sun-Ah;Jo, Nam-Kyu;Lee, Sung-Mo
    • Korean Journal of Pharmacognosy
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    • v.48 no.1
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    • pp.56-62
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    • 2017
  • The aim of this study was to screen the contamination by ochratoxin A of mycotoxins in various medicinal herbs. We conducted a survey of ochratoxin A in medicinal herb on the retail market in Incheon in 2016. 116 medicinal herb samples were evaluated for the ochratoxin A contamination. They were analyzed for ochratoxin A using immunoaffinity column and high-performance liquid chromatography(HPLC)-fluorescence detection and the positive samples were confirmed using HPLC-tandem mass spectrometry. Ochratoxin A was detected in 4 medicinal herb samples; the concentrations of ochratoxin A were containing between 20.11 and $372.90{\mu}g/kg$. This study shows that in general, this kind of commodity may be contaminated by mycotoxins. Also this contamination is not limited to only aflatoxin of mycotoxins.

A Survey of Aflatoxin Contamination in Medicinal Herbs for Food and Medicine (식약공용한약재의 아플라톡신 오염실태 조사)

  • Jo, Sung-Ae;Lee, Sung-Deuk;Kim, Dong-Gyu;Lee, Hyun-Kyung;Jung, Sun-Ok;Kim, Kyung-Sik;Yoo, ln-Sil;Jung, Kweon
    • Korean Journal of Pharmacognosy
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    • v.45 no.2
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    • pp.154-160
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    • 2014
  • The aim of this study was to determine the incidence and contamination levels of aflatoxin in Medicinal Herbs for Food and Medicine at Yakyeang market in seoul. 191 Samples 11 items medicinal herbs for food and medicine were evaluated for the aflatoxin contamination. in result 41 samples 10 items (21.5%) were detected in the alfatoxin, a high incidence of aflatoxins items are cassiae semen (50.0%), testudinis plastrum (43.8%) and Batryticatus Bombyx (40.0%), Polygalae Radix (31.2%), Zizyphi Semen (23.5%), Dolichoris Semen, Myristicae Semen (20.0%), Nelumbinis Semen (15.8%), Glycyrrhizae Radix et Rhizoma (7.4%), Hoveniae Semen Cum Fructus (4.3%). AFB1 were detected 27 cases (14.1%), AFB2, AFG1 and AFG2 were detected 18cases (9.4%), 16cases (8.4%) and 5cases (2.6%). The excess cancer risk estimated using the cancer potency of aflatoxin B1 (7(mg/kg/day)-1 for HBsAg-and 230(mg/kg/day)-1 HBsAg+) was N.D ~ $3.79{\times}10^{-6}$ for hepatits B surface antigen negative (HBsAg-) and N.D ~ $9.68{\times}10^{-5}$ hepatits B surface antigen positive (HBsAg+) respectively.

Characteristics of Yersinia enterocolitica isolates from beef and pork carcass (소와 돼지도체에서 Yersinia enterocolitica의 분리 및 특성)

  • Chae, Hee-Sun;Kim, Joo-Young;Kim, Jee-Eun;Yang, Yun-Mo;Jin, Kyung-Sun;Shin, Bang-Woo;Kim, Sun-Heung;Lee, Jung-Hark
    • Korean Journal of Veterinary Service
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    • v.31 no.2
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    • pp.195-205
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    • 2008
  • Yersinia enterocolitica is a zoonotic agent, and to cause food poisoning. This study was carried out to get some basic information for the control of Yersinia infection. A total of 1,680 samples were collected from beef and pork carcasses from January 2006 to December 2007 in Seoul. The isolation rate was higher in pork carcass than in beef carcass. Five (0.59%) Yersinia enterocolitica were isolated from the 840 of beef carcasses, and eighteen(2.14%) were isolated from the 840 of pork carcasses. Among 23 strains, 22 were classified into biotype 1A, and one was biotype 6. In serotyping of Y enterocolitica isolates, 21 strains were untypable (UT), and 2 were O5 and O8 respectively. In PCR, Ail gene was not detected in all of 23 strains that determined non-pathogenic. In antimicrobial susceptibility test, twelve strains (52.2%) of 23 isolates showed the multi -resistant patterns with over 3 drugs. PFGE was performed after the genomic DNA of twenty three isolates, which was digested with Xba I. the 23 isolates showed 12 ($A{\sim}L$) PFGE type.

Distribution of Antibiotic Resistant Escherichia coli and Water Quality in Waterscape Facilities (물놀이형 수경시설의 수질과 항생제 내성 대장균 분포에 관한 연구)

  • Yoon, Sang-Hoon;Kim, Seon-Gyeong;Kim, Nan-Hee;Wi, Whan;Lee, Se-Hang;Jo, Gwang-Woon;Kim, Dong-Su;Cho, Young-Gwan
    • Journal of Environmental Health Sciences
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    • v.40 no.3
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    • pp.225-233
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    • 2014
  • Objective: This study was performed in order to investigate the distribution of antibiotic resistant E. coli and water quality in waterscape facilities. Methods: Nine waterscape facilities were selected and classified into three types: ground, wall and general fountain. The items analyzed in this study were pH, turbidity, $KMnO_4$ consumption, total colony, total coliforms, fecal coliforms and E. coli. Additionally, an antibiotic resistance test on E. coli was performed. Results: There were no sampling sites that exceeded the permitted limits of water quality, but the concentration of pollutants was high at the beginning operation in June. Moreover, concentration of turbidity, $KMnO_4$ consumption and total colony were higher in ground fountains than in wall and general fountains. Five species of antibiotic resistant E. coli were detected from three sampling sites. Conclusions: The waterscape facilities were significantly contaminated with water microorganisms, especially total colony, total coliforms and fecal coliforms. Disinfection and filtration systems to remove pollutants should be installed for safe waterscape facilities.

Simultaneous Analysis of Mycotoxins and Risk Assessment in Seeds using LC-MS/MS (LC-MS/MS를 이용한 종자류 생약의 곰팡이독소 동시분석 및 위해도 평가)

  • Choi, Eun Jung;Park, Young Ae;Choi, Su Jeong;Jung, Sam Ju;Park, Youn Sun;Hwang, In Sook;Yu, In Sil;Shin, Gi Young
    • Korean Journal of Pharmacognosy
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    • v.51 no.4
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    • pp.270-277
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    • 2020
  • This study analyzed mycotoxins, aflatoxin B1, B2, G1, G2, fumonisin B1, B2, ochratoxin A and zearalenone, using LC-MS/MS and conducted risk assessment on 54 samples of seeds distributed in SeoulYangnyeongsi and the management status of extramural herbal dispensary facility. The matched calibration showed a good linearity as observed in 6 concentration levels(r2>0.999) as a result of method validation applied with Arecae semen. Limits of detection(LOD) and quantification(LOQ) were in the range of 0.02-0.11 ㎍/kg and 0.08-0.34 ㎍/kg, respectively. Recoveries also estimated, ranging from 65.1-99.7% with relative standard deviation(RSD) 0.5-6.3%. As a result of the method on 54 samples, mycotoxins were detected in 16 samples. Among them, two Thujae semen showed a degree of concentration that exceeded the aflatoxin specification. In the risk assessment, the human exposure safety standard values were calculated as ADI(Acceptable Daily Intake) for aflatoxin B1, fumonisin and zearalenone. Ochratoxin A was calculated as PTWI(Provisional Tolerable Weekly Intake). The MOE(Margine of Exposure) of aflatoxin B1 was in the range of 40.36-3536.88. And no items exceeded 100% in %TDI(Tolerable Daily Intake) and %TWI(Tolerable Weekly Intake) of fumonisin, zearalenone and ochratoxin A.

A Comparison of Performance Evaluations of Reusable Masks (다회용 마스크 유형에 따른 성능 비교연구)

  • Ryu, Hoe-Jin;Park, Won-Hee;Lee, Chun-Yeong;Jeong, Seon-Ok;Choi, Hee-Jin;Lee, Jeong-Mi;Hwang, In-Sook;Shin, Yong-Seung
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.32 no.1
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    • pp.53-63
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    • 2022
  • Objectives: This study was designed to evaluate the filtration efficiency, inhalation resistance, and liquid resistance of commercial reusable masks in comparison to the performance of KF-AD masks (reference masks). Methods: Thirty-six masks were purchased from an online market. Filtration efficiency and inhalation resistance were determined in the same manner as in MFDS certification testing, respectively using TIL/IL & Filter Test 1300S (SFP Services, UK) and Breathing Resistance Analyzer DA2001 (Lambda Ray, South Korea). Results: Most of the filtration efficiencies found for the 19 masks were far lower than the test standard for KF-certified masks. Four masks met KF94 and three masks even met KF80. Most inhalation resistances were also much lower than the standard, with many almost one-half of the standard. In addition, all the masks except KF-AD masks did not pass the liquid resistance test. Conclusions: Although most of the filtration efficiency performance results found in this study for the reusable masks, which emerged as an alternative, were lower than the standard for health masks, multi-use masks can be used with a replacement filter inserted.