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http://dx.doi.org/10.22889/KJP.2020.51.4.270

Simultaneous Analysis of Mycotoxins and Risk Assessment in Seeds using LC-MS/MS  

Choi, Eun Jung (Seoul Metropolitan Government Research Institute of Public Health and Environment)
Park, Young Ae (Seoul Metropolitan Government Research Institute of Public Health and Environment)
Choi, Su Jeong (Seoul Metropolitan Government Research Institute of Public Health and Environment)
Jung, Sam Ju (Seoul Metropolitan Government Research Institute of Public Health and Environment)
Park, Youn Sun (Seoul Metropolitan Government Research Institute of Public Health and Environment)
Hwang, In Sook (Seoul Metropolitan Government Research Institute of Public Health and Environment)
Yu, In Sil (Seoul Metropolitan Government Research Institute of Public Health and Environment)
Shin, Gi Young (Seoul Metropolitan Government Research Institute of Public Health and Environment)
Publication Information
Korean Journal of Pharmacognosy / v.51, no.4, 2020 , pp. 270-277 More about this Journal
Abstract
This study analyzed mycotoxins, aflatoxin B1, B2, G1, G2, fumonisin B1, B2, ochratoxin A and zearalenone, using LC-MS/MS and conducted risk assessment on 54 samples of seeds distributed in SeoulYangnyeongsi and the management status of extramural herbal dispensary facility. The matched calibration showed a good linearity as observed in 6 concentration levels(r2>0.999) as a result of method validation applied with Arecae semen. Limits of detection(LOD) and quantification(LOQ) were in the range of 0.02-0.11 ㎍/kg and 0.08-0.34 ㎍/kg, respectively. Recoveries also estimated, ranging from 65.1-99.7% with relative standard deviation(RSD) 0.5-6.3%. As a result of the method on 54 samples, mycotoxins were detected in 16 samples. Among them, two Thujae semen showed a degree of concentration that exceeded the aflatoxin specification. In the risk assessment, the human exposure safety standard values were calculated as ADI(Acceptable Daily Intake) for aflatoxin B1, fumonisin and zearalenone. Ochratoxin A was calculated as PTWI(Provisional Tolerable Weekly Intake). The MOE(Margine of Exposure) of aflatoxin B1 was in the range of 40.36-3536.88. And no items exceeded 100% in %TDI(Tolerable Daily Intake) and %TWI(Tolerable Weekly Intake) of fumonisin, zearalenone and ochratoxin A.
Keywords
Mycotoxin; LC-MS/MS; Seeds;
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