• Korean Journal of Microbiology
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• v.38 no.1
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• pp.38-44
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• 2002

Pneumococcal surfacce protein A (PspA) is an important virulence factor and an antigenically variable surface protein of the pneumococci. To purify the PspA from S. pneumoniae KNIH1156 , a clinical isolate (type 19F), we have taken advantage of the fact that PspA is released from the surface of pneumococci into the medium by growing in a CDM-ET medium and PspA is capable of binding human lactoferrin, the iron carrier protein. PspA of S. pneumoniae KNIH1156 was purified from culture supernatant by human lactoferrin (hLf) affinity chromatography. The purified PspA was confirmed with anti-PspA antiserum and also had the binding capacity to hLf specifically. To determine whether the purified PspA could elicit protection in mice against pneumococcal inflection, we immunized the mice with purified PspA and subsequently challenged with S. pneumoniae KNIH1156. Immunization with purified PspA protected mice from 500 times the $LD^{50}$ of S. pneumoniae KNIH1156. Therefore, it has been shown that purified PspA fromS. pneumoniae KNIH1156 (type 19F) is a protective immunogen.

• Clinical and Experimental Pediatrics
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• v.48 no.11
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• pp.1206-1211
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• 2005

Purpose : Pneumococcal protein vaccine based on pneumococcal surface protein A (PspA) is in development with the potential to offer a broad range of protection against different strains. PspA elicits protection in mice against fatal sepsis as well as carriage and lung infection. This study was performed to investigate the frequency of PspA families among Streptococcus pneumoniae recovered from Korean children and adults. Methods : A total of 89 pneumococcal isolates was included in the study. They were capsule serotyped by the slide agglutination assay with commercial antisera. PspA families were determined with polymerase chain reaction using the pair of primers for family 1 and family 2. Results : Seventeen pneumococcal serotypes were found in a total of 89 isolates. PspA typing was able to ascertain 79 of the 89 isolates (88.8 percent). Among these, 20 (22.5 percent) isolates were family 1 PspA, 59 (66.3 percent) were family 2. Moreover, because 9 (10.1 percent) isolates were of positive reactions for both, families 1 and 2 primers, the potential coverage of PspA vaccine was 98.9 percent. PspA families were not associated with age group, source of isolates, or penicillin susceptibility. However, the relative distribution of family 1 isolates to family 2 isolates was significantly different over capsular serotypes. Conclusion : The finding that 98.9 percent of Korean isolates belonging to PspA families 1 and 2 support the hypothesis that a human PspA vaccine covering a few PspA families could be broadly effective. The monitoring of the PspA families derived from large population-based isolates will be necessary in the context of vaccine development.

• Journal of the Korean Society of Propulsion Engineers
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• v.12 no.1
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• pp.7-15
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• 2008

Pressure Sensitive Paint(PSP) means a reacting paint in pressure. The calibration of PSP and the wind tunnel test of PSP painted model are required to measure pressure by using PSP. Therefore, the post processing from these results shows the information and image of the pressure distribution. PSP can show the information of total pressure from the wind tunnel test and the calibration. In this study, equipments of PSP are composed, and experiment is accomplished by using PSP. The surface pressure distribution around the wall of nozzle is measured by PSP. The measured pressure has similar results to those of the CFD and pressure tap measurement.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2007.11a
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• pp.99-102
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• 2007

Pressure Sensitive Paint(PSP) means a reacting paint in pressure. The calibration of PSP and the wind tunnel test of PSP painted model are required to measure pressure using by PSP. Therefore, the post processing from these results shows the information and image of the pressure distribution. PSP can show the information of total pressure from the wind tunnel test and the calibration. In this study, equipments of PSP are composed, and calibration is accomplished using by PSP. Results of design and manufacturing calibration chamber till ability of advance calibration system study.

• Journal of the Korean Society of Food Science and Nutrition
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• v.33 no.2
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• pp.278-286
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• 2004

The objective of the current study was to determine the effects of arabinoxylane and PSP on mouse splenocytes, T cells, B cells and macrophages in vitro. Arabinoxylane and PSP directly induced the proliferation of spleen cells in a dose-dependent manner and increased IFN-${\gamma}$ synthesis. Especially, PSP induced IL-2, IL-4 and IL-10 production, Both arabinoxylane and PSP increased PFC (plaque forming cell) and RFC (rosette forming cell) formation. Arabinoxylane was not induced the proliferation of T cells, but PSP directly induced the proliferation of T cells in a high dose. Arabinoxylane and PSP increased the proliferation of B cells and the phagocytic effects of macrophage. When arabinoxylane and PSP were used in macrophage cell line stimulation, there was a marked induction of NO synthesis in a dose-dependent and an increased TNF-$\alpha$ and IL-6 synthesis. Especially, PSP also induced IL-1$\beta$ production. When arabinoxylane and PSP treated in macrophage cell line, there was induction of MHC class II expression. These results suggest that the capacity of arabinoxylane andPSP seem to act as a potent immunomodulator causing augmentation of immune cell activity, and with the absence of notable side-effects, arabinoxylane and PSP could be used as a biological response modifier having possible therapeutic effects against immunological disorders.

• Journal of the Korean Society of Food Science and Nutrition
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• v.33 no.3
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• pp.469-474
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• 2004

The objective of the current study was to determine the effects of arabinoxylane and polysaccharide peptide (PSP) on the immune cell functions. Both arabinoxylane and PSP increased plaque forming cell (10-15%) and rosette forming cell (10-30%) formation. Stimulation of macrophage with PSP (18%) and arabinoxylane (22%) resulted in increased phagocytic effects. Both arabinoxylane and PSP induced the tumor suppressive effect in mice injected sarcoma-180 cell intracutaneously. When the mice injected intraperitoneal cavity with sarcoma 180 cells, survival ratio was increased in the mice fed on arabinoxylane and PSP. The ratio of PCA was slightly decreased in the mice fed on PSP, especially fed on arabinoxylane than in the control mice. The concentrations of blood histamine were slightly decreased in the mice fed on arabinoxylane and PSP. These results suggest that the capacity of arabinoxylane and PSP seems to act as a potent immunomodulator causing augmentation of immune cell activity, and with the absence of notable side-effects, arabinoxylane and PSP could be used as a biological response modifier having possible therapeutic effects against immunological disorders.

• Journal of the Korean Solar Energy Society
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• v.30 no.5
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• pp.25-31
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• 2010

A Precision Spectral Pyranometer (PSP) is mainly used as a reference to calibrate other pyranometers due to its high accuracy and sensitivity in response to the spectrum wavelength range of 0.285 ${\mu}$ to 2.8 ${\mu}$, while the sensitivity of photovoltaic-type Li-Cor pyranometer is limited within a certain spectral range from 0.4 ${\mu}$ to 1.1 ${\mu}$. In this study, two Eppley PSPs($PSP_1$ and $PSP_2$) were first compared to the calibrated Eppley PSPs from National Renewable Energy Laboratory (NREL), resulting in two linear correction factors based on the comparison between the logger output (V) from the test PSP and the solar radiation (W/m2) from the NREL PSP. The Li-Cor pyranometer used in this study was then corrected based on the comparison of measured solar radiation ($W/m^2$) from the corrected $PSP_1$ and the Li-Cor pyranometer. In addition, instrument scale corrections were also performed for the PSPs and the Li-Cor from the transmitter to the data logger. From the comparisons, a linear correction factor (1.0214) with R=0.9998 was developed for the scale correction between$PSP_1$ and $PSP_2$, while the Li-Cor pyranometer has a scale(1.0597) and offset (32.046) with R=0.9998 against$PSP_1$. As a result, it was identified that there were good agreements within ${\pm}$ 10 W/ $m^2$ between Eppley $PSP_1$ vs. $PSP_2$ solar radiation and within ${\pm}$ 20 W/$m^2$ between$PSP_1$ vs Li-Cor solar radiation after the empirical scale corrections developed in this study.

• The Journal of Korean Association of Computer Education
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• v.9 no.4
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• pp.55-61
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• 2006

The Personal Software Process(PSP) is a rigorous, measurements based process designed to help software developers produce high-quality codes. Learning PSP requires substantial and tedious record keeping including clerical computations with plenty of data occurred at every project. The required task reduces the educational effect and motive by time consuming characteristic and easy occurrence of errors. To alleviate these problems, a web-based PSP log system having streamlined mechanism of data manipulation is designed and implemented at this study. Three years of experiment shows that the system not only improves productivity and accuracy of the process but also allows students and instructors to focus on learning and teaching PSP, rather than PSP data collection.

• The Korean Journal of Nuclear Medicine
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• v.21 no.2
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• pp.175-182
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• 1987

For measurement of ventricular performance, ejection fraction (EF) has gained wide acceptance. But EF is influenced not only by changes in muscle function but also by changes in cardiac loading conditions. In case of valvular heart disease which is variable in loading conditions, EF cannot be reliable as an index of myocardial contractility. The end systolic pressure (ESP)-end systolic volume (ESV) relation, howver, is known to represent myocardial contractility, independent of changes in loading conditions. Similar results can be obtained by using peak-systolic pressure (PSP) instead of ESP. To evaluate the utility of the peak systolic pressure-end systolic volume index (PSP-ESVI) relation as an index of myocardial function, we measured $PSP&ESVI$ in 19 partents with coronary artery disease before $(PSP_1\;&\;ESVI_1)$ and after $(PSP_2\;&\;ESVI_2)$ sublingual administration of nitroglycerin. PSP was measured with standard mercury sphygmomanometer during gated blood pool scintigraphic study. ESVI was measured by count derived method after attenuation correction. $PSP_2\;&\;ESVI_2$ measurement was started when the fall of PSP was greater than 5 mmHg after 7-14 minutes post-administration of nitroglycerin. Mean values $({\pm}S.D.)$ of $PSP_1\;&\;ESVI_1$ was $124.9({\pm}20.7)mmHg\;&\;59.4({\pm}39.9)ml/M^2$. Mean values $({\pm}S.D)$ of $PSP_2\;&\;ESVI_2$, was $113.2({\pm}19.9)mmHg\;&\;37.5({\pm}26.1)ml/M^2$. There was a significant difference between mean values of $PSP_1\;&\;PSP_2$, (p<0.01), and mean values of $ESVI_1\;&\;ESVI_2$, (p<0.01). $PSP_1-PSP_2/ESV_1-ESVI_2,\;PSP_1/ESVI_1$ and EF were in the range of 0.14-5.19 mmHg/ml/$M^2$, 0.67-7.68 mmHg/ml/$M^2$ and 10.8%-74.5% respectively. $PSP_1-PSP_2/ESVI_1-ESVI_2$, and EF showed exponential correlation (r=0.85, P<0.01). The correlation coefficient between $PSP_1/ESVI_1$ and EF was 0.73(p<0.01). With the above results, we suggest that $PSP_1-PSP_2/ESVI_1-ESVI_2$, and $PSP_1/ESVI_1$, can be used as an index of myocardial function.

• 한국가시화정보학회:학술대회논문집
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• 2004.11a
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• pp.10-13
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• 2004

The PSP(pressure sensitive paint) technique has recently received a large attention as a new revolutionary optical method to measure absolute pressure distribution on a model surface. The PSP technique can be applied to quantitatively investigate flow structure using a CCD camera and image processing technique. In the static calibration, the luminescent intensity of PSP coatings was measured from 0kPa to 11kPa with 0.5, 1, 2kPa increments. In this study, the low-pressure PSP technique was applied to an oblique impinging jet to measure pressure field variations on the impingement plate with varying angle of an oblique jet. The flow structure over the impingement plate was visualized using a surface tracing method. As a result, the detail pressure field distributions of the oblique low-speed impinging jet were visualized effectively using the PSP technique.