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http://dx.doi.org/10.3746/jkfn.2004.33.2.278

Effect of Arabinoxylane and PSP on Activation of Immune Cells  

채수연 (순천대학교 생물학과)
신성해 (순천대학교 생물학과)
배만종 (대구한의대학교 한방식품과학부)
박미현 (이롬라이프 생명과학연구원)
송미경 (이롬라이프 생명과학연구원)
황성주 (이롬라이프 생명과학연구원)
이성태 (순천대학교 생물학과)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.33, no.2, 2004 , pp. 278-286 More about this Journal
Abstract
The objective of the current study was to determine the effects of arabinoxylane and PSP on mouse splenocytes, T cells, B cells and macrophages in vitro. Arabinoxylane and PSP directly induced the proliferation of spleen cells in a dose-dependent manner and increased IFN-${\gamma}$ synthesis. Especially, PSP induced IL-2, IL-4 and IL-10 production, Both arabinoxylane and PSP increased PFC (plaque forming cell) and RFC (rosette forming cell) formation. Arabinoxylane was not induced the proliferation of T cells, but PSP directly induced the proliferation of T cells in a high dose. Arabinoxylane and PSP increased the proliferation of B cells and the phagocytic effects of macrophage. When arabinoxylane and PSP were used in macrophage cell line stimulation, there was a marked induction of NO synthesis in a dose-dependent and an increased TNF-$\alpha$ and IL-6 synthesis. Especially, PSP also induced IL-1$\beta$ production. When arabinoxylane and PSP treated in macrophage cell line, there was induction of MHC class II expression. These results suggest that the capacity of arabinoxylane andPSP seem to act as a potent immunomodulator causing augmentation of immune cell activity, and with the absence of notable side-effects, arabinoxylane and PSP could be used as a biological response modifier having possible therapeutic effects against immunological disorders.
Keywords
arabionxylane; polysaccharide-peptide (PSP); cytokines; nitric oxide; MHC class;
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